Scientia Agricultura Sinica ›› 2010, Vol. 43 ›› Issue (11): 2363-2371 .doi: 10.3864/j.issn.0578-1752.2010.11.021

• VETERINARY SCIENCE • Previous Articles     Next Articles

Polymorphisms of MBL1 Gene Intron 1 and Exon 2 and Its Association with Mastitis and Milk Quality in Chinese Holstein Cattle

LIU Mei, JU Zhi-hua, LI Qiu-ling, WANG Hong-mei, HUANG Jin-ming, LI Jian-bin, ZHONG Ji-feng, WANG Chang-fa
   

  1. （山东省农业科学院奶牛研究中心）

  • Received:2009-09-23 Revised:2010-01-22 Online:2010-06-01 Published:2010-06-01
  • Contact: WANG Chang-fa

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Abstract:

【Objective】 The polymorphisms of MBL1 gene intron1 and exon2 and their correlation to mastitis and milk quality in Chinese Holstein cattle were investigated for the purpose of providing molecular maker information to facilitate the breeding efficiency of high-quality resistant cow. 【Method】 Sequencing, PCR-RFLP and CRS-PCR were applied to analyze the polymorphisms of three loci in MBL1 gene intron1 and exon2 in 596 Chinese Holstein cattle. Linkage disequilibrium and haplotype were analyzed using SHEsis Software. 【Result】 One SNP in the intron1 G855A and two SNPs in the exon2 G2651A (Val24Ile), T2686C (Ala35Ala) were found.At locus G2651A, the cows with genotype AA showed higher somatic cell score than those with genotypes GG and GA (P＜0.05), but there were no correlation between the SNP G855A, T2686C and somatic cell score, fat rate, protein rate and 305 d milk yield. Eight haplotype and 19 haplotype combinations were found in 596 Chinese Holstein cattle. The cows with haplotype combination H2H2 had the highest protein rate and somatic cell score. Haplotype combination H3H7 had the lowest somatic cell score. The cows with haplotype combinations H4H4 had the highest 305 d milk yield. 【Conclusion】 Considering the distribution frequencies of haplotype combinations, MBL1 gene H3H7 is a favorable haplotype combinations to the mastitis resistance selection and H4H4 can be chosen as the molecular markers for choosing high milk yield.

Key words: China Holstein, MBL1 gene, polymorphism, mastitis, milk quality

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