Scientia Agricultura Sinica ›› 2010, Vol. 43 ›› Issue (11): 2363-2371 .doi: 10.3864/j.issn.0578-1752.2010.11.021

• VETERINARY SCIENCE • Previous Articles     Next Articles

Polymorphisms of MBL1 Gene Intron 1 and Exon 2 and Its Association with Mastitis and Milk Quality in Chinese Holstein Cattle

LIU Mei, JU Zhi-hua, LI Qiu-ling, WANG Hong-mei, HUANG Jin-ming, LI Jian-bin, ZHONG Ji-feng, WANG Chang-fa
   

  1. （山东省农业科学院奶牛研究中心）

  • Received:2009-09-23 Revised:2010-01-22 Online:2010-06-01 Published:2010-06-01
  • Contact: WANG Chang-fa

PDF

658

Abstract:

【Objective】 The polymorphisms of MBL1 gene intron1 and exon2 and their correlation to mastitis and milk quality in Chinese Holstein cattle were investigated for the purpose of providing molecular maker information to facilitate the breeding efficiency of high-quality resistant cow. 【Method】 Sequencing, PCR-RFLP and CRS-PCR were applied to analyze the polymorphisms of three loci in MBL1 gene intron1 and exon2 in 596 Chinese Holstein cattle. Linkage disequilibrium and haplotype were analyzed using SHEsis Software. 【Result】 One SNP in the intron1 G855A and two SNPs in the exon2 G2651A (Val24Ile), T2686C (Ala35Ala) were found.At locus G2651A, the cows with genotype AA showed higher somatic cell score than those with genotypes GG and GA (P＜0.05), but there were no correlation between the SNP G855A, T2686C and somatic cell score, fat rate, protein rate and 305 d milk yield. Eight haplotype and 19 haplotype combinations were found in 596 Chinese Holstein cattle. The cows with haplotype combination H2H2 had the highest protein rate and somatic cell score. Haplotype combination H3H7 had the lowest somatic cell score. The cows with haplotype combinations H4H4 had the highest 305 d milk yield. 【Conclusion】 Considering the distribution frequencies of haplotype combinations, MBL1 gene H3H7 is a favorable haplotype combinations to the mastitis resistance selection and H4H4 can be chosen as the molecular markers for choosing high milk yield.

Key words: China Holstein, MBL1 gene, polymorphism, mastitis, milk quality

[1] LIN Ping, WANG KaiLiang, YAO XiaoHua, REN HuaDong. Development of DNA Molecular ID in Camellia oleifera Germplasm Based on Transcriptome-Wide SNPs [J]. Scientia Agricultura Sinica, 2023, 56(2): 217-235.
[2] XIA YuXin,LIANG Yan,WANG HaiYang,GUO MengLing,ZHOU Bu,DAI Xu,YANG ZhangPing,MAO YongJiang. Effects of the Number of Subclinical Mastitis and Somatic Cell Score in Milk of Parity 1 on Somatic Cell Score of Holstein Cows for Parity 2 [J]. Scientia Agricultura Sinica, 2022, 55(20): 4052-4064.
[3] XiaoChuan LI,ChaoHai WANG,Ping ZHOU,Wei MA,Rui WU,ZhiHao SONG,Yan MEI. Deciphering of the Genetic Diversity After Field Late Blight Resistance Evaluation of Potato Breeds [J]. Scientia Agricultura Sinica, 2022, 55(18): 3484-3500.
[4] LIANG Peng,ZHANG TianWen,MENG Ke,SHAO ShunCheng,ZOU ShiFan,RONG Xuan,QIANG Hao,FENG DengZhen. Association Analysis of the ADIPOQ Variation with Sheep Growth Traits [J]. Scientia Agricultura Sinica, 2022, 55(11): 2239-2256.
[5] XU ZhiYing,WANG BaiCui,MA XiaoLan,JIA ZiMiao,YE XingGuo,LIN ZhiShan,HU HanQiao. Polymorphism Analysis Among Chromosomes of Dasypyrum villosum 6V#2 and 6V#4 and Wheat 6A and 6D Based on Wheat SNP Chip [J]. Scientia Agricultura Sinica, 2021, 54(8): 1579-1589.
[6] ZHANG PengFei,SHI LiangYu,LIU JiaXin,LI Yang,WU ChengBin,WANG LiXian,ZHAO FuPing. Advance in Genome-Wide Scan of Runs of Homozygosity in Domestic Animals [J]. Scientia Agricultura Sinica, 2021, 54(24): 5316-5326.
[7] CHEN Zhi,ZHANG Yi,LU QinYue,GUO JiaHe,LIANG Yan,ZHANG MingYiXing,YANG ZhangPing. Effect and Mechanism of Tea Tree Oil on LPS Induced Mastitis in Dairy Cows [J]. Scientia Agricultura Sinica, 2021, 54(14): 3124-3133.
[8] SONG MeiJie,OU AiQun,XUE XiaoFeng,WU LiMing,SHOU QiYang,WANG Kai. Protective Effects of Chinese Propolis Extract Against Lipopolysaccharide- Induced Acute Mastitis and Mammary Barrier Functions in Mice [J]. Scientia Agricultura Sinica, 2021, 54(12): 2675-2688.
[9] Yun PENG,TianGang LEI,XiuPing ZOU,JingYun ZHANG,QingWen ZHANG,JiaHuan YAO,YongRui HE,Qiang LI,ShanChun CHEN. Verification of SNPs Associated with Citrus Bacterial Canker Resistance and Induced Expression of SNP-Related Calcium-Dependent Protein Kinase Gene [J]. Scientia Agricultura Sinica, 2020, 53(9): 1820-1829.
[10] XU Yunbi,YANG QuanNü,ZHENG HongJian,XU YanFen,SANG ZhiQin,GUO ZiFeng,PENG Hai,ZHANG Cong,LAN HaoFa,WANG YunBo,WU KunSheng,TAO JiaJun,ZHANG JiaNan. Genotyping by Target Sequencing (GBTS) and Its Applications [J]. Scientia Agricultura Sinica, 2020, 53(15): 2983-3004.
[11] CHEN XiaoHong,HE JieLi,SHI TianTian,SHAO HuanHuan,WANG HaiGang,CHEN Ling,GAO ZhiJun,WANG RuiYun,QIAO ZhiJun. Developing SSR Markers of Proso Millet Based on Transcriptome Sequencing [J]. Scientia Agricultura Sinica, 2020, 53(10): 1940-1949.
[12] LI GuangDong,LÜ DongYing,TIAN XiuZhi,JI PengYun,GUO JiangPeng,LU YongQiang,LIU GuoShi. Research Progress of Omics Technologies in Cow Mastitis [J]. Scientia Agricultura Sinica, 2019, 52(2): 350-358.
[13] CAO XueTao, PEI ShengWei, ZHANG Jin, LI FaDi, LI Gang, LI WanHong, YUE XiangPeng. Screening of Y Chromosome Specific Primers and Y-SNPs in Sheep [J]. Scientia Agricultura Sinica, 2018, 51(15): 2990-2999.
[14] WANG Xiao, ZHANG Qin, YU Ying. Genome-Wide Association Study on Mastitis Resistance Based on Somatic Cell Scores in Chinese Holstein Cows [J]. Scientia Agricultura Sinica, 2017, 50(4): 755-763.
[15] GAO XiaoLi, HU Jiang, GUO ShuZhen, SHI BinGang, XIE JianPeng, LUO YuZhu, WANG JiQing, MU YongJuan. Polymorphisms of DGAT1 Gene and Their Association with Milk Quality Traits in Yak [J]. Scientia Agricultura Sinica, 2017, 50(16): 3215-3225.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd