Scientia Agricultura Sinica ›› 2009, Vol. 42 ›› Issue (11): 3755-3764 .doi: 10.3864/j.issn.0578-1752.2009.11.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Progress in Plant Small RNA Research via High-Throughput Sequencing

WEI Bo, ZHANG Rong-zhi, LI Ai-li, MAO Long
  

  1. (中国农业科学院作物科学研究所/国家农作物基因资源与遗传改良重大科学工程)
  • Received:2009-01-16 Revised:2009-02-24 Online:2009-11-10 Published:2009-11-10
  • Contact: MAO Long

Abstract:

Small RNAs are 20-30 nucleotides long non-coding RNAs. Small RNA-mediated post-transcriptional regulation represents a new mechanism of gene regulation and plays important roles in plant growth and development as well as responses to different environmental stresses. In plants, there are tremendous amount of small RNAs of various types. The advance of high- throughput sequencing technology has significantly facilitated their discoveries. In this review, in addition to a brief introduction of small RNA features, the authors attempted to summarize recent progresses in small RNA research using next generation sequencing technology and aim to highlight the new insights in their genome organization, function and evolution in plants.

Key words: high-throughput sequencing, 454-FLX, Solexa, small RNA, microRNA, siRNA

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