Scientia Agricultura Sinica ›› 2024, Vol. 57 ›› Issue (2): 416-428.doi: 10.3864/j.issn.0578-1752.2024.02.015

• RESEARCH NOTES • Previous Articles    

Characteristics of Endophytic Microbial Community Structures in Stems Between Hylocereus undatus and H. polyrhizus

ZHOU XinYan1(), CHEN SiYu1, WEI YuFei1, ZHU Yu1, FENG JunQian1, DING DianCao1, LU GuiFeng2(), YANG ShangDong1()   

  1. 1 College of Agriculture, Guangxi University/Guangxi Key Laboratory of Agricultural Products Safety/National Experimental Teaching Demonstration Center of Plant Science, Nanning 530004
    2 Horticultural Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007
  • Received:2023-05-05 Accepted:2023-06-09 Online:2024-01-16 Published:2024-01-19
  • Contact: LU GuiFeng, YANG ShangDong

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Abstract:

【Objective】 The differences of the endophytic microbial community structures between Hylocereus undatus and H. polyrhizus were analyzed, so as so to explore the correlation between the pulp color of H. polyrhizus and the endophytic microbial community composition and their functional microorganisms. 【Method】 Based on high-throughput sequencing technology, the diversity and richness of endophytic microorganisms (bacteria and fungi) in stems between H. undatus and H. polyrhizus were analyzed. Meanwhile, based on LEfSe analysis, the differences of endophytic microorganisms in stems between H. undatus and H. polyrhizus were also investigated.【Result】 The significant differences of the endophytic microbial community structures were found in stems between H. undatus and H. polyrhizus. Meanwhile, the numbers of specific bacterial and fungal OTUs in stems of H. polyrhizus were all higher than those of H. undatus. At the phylum level, the relative abundance ratio of Ascomycota in stems of H. polyrhizus was 1.15 times higher than that of H. undatus. At the genus level, Streptomyces and Penicillium were the highest abundant dominant bacterial and fungal genera in stems of H. polyrhizus, which were 1.24 and 4.27 times higher than those of H. undatus, respectively. In addition, some bacterial genera, such as Lechevalieria, Glycomyces, unclassified_f__Enterobacteriaceae, Actinomadura, and some fungal gerera, such as Talaromyces, unclassified_f__Serendipitaceae, unclassified_c__GS13, unclassified_o__Atractiellales, unclassified_o__Auriculariales were enriched in stems of H. polyrhizus. LEfSe analysis also showed that Promicromonospora and Xylomyces were significant enriched in stems of H. polyrhizus. 【Conclusion】 All above results suggested that the formation of pigment was closely related to the compositions of endophytic microbial community in stems of H. polyrhizus. The bacterial genera, such as Streptomyces, unclassified_f__Enterobacteriaceae, Promicromonospora, and the fungal phylum and genera, such as Ascomycota, Penicillium, Talaromyces and Xylomyces, were all the potential microorganisms in relating to pigment synthesis and metabolic accumulation in stems of H. polyrhizus.

Key words: Pitaya (Hylocereus undulatus Britt), pulp color, endophytic microorganisms, high-throughput sequencing

Table 1

Sequencing types and primer sequences"

引物名称
Primer name		 引物类型
Primer type		 引物序列
Primer sequence (5′→3′)		 测序平台
Sequencing platform		 序列长度
Sequencing length (bp)
799F
1193R		 内生细菌
Endophytic bacteria		 AACMGGATTAGATACCCKG
ACGTCATCCCCACCTTCC		 MiSeq 394
ITS1F 内生真菌
Endophytic fungi		 CTTGGTCATTTAGAGGAAGTAA 242
ITS2R GCTGCGTTCTTCATCGATGC

Table 2

Numbers of endophytic microorganisms in stems between H. undatus and H. polyrhizus cultivars at different taxonomic levels"

处理
Treatment		 OTU数量
Numbers of operational taxonomic units		 不同分类类别数量 Numbers of different taxonomic categories
门
Phylum		 纲
Class		 目
Order		 科
Family		 属
Genus		 种
Species
内生细菌Endophytic bacteria 白肉RW 466 18 33 93 149 262 366
红肉RR 491 19 35 95 157 274 377
总计Total 555 21 40 106 174 303 424
内生真菌Endophytic fungi 白肉RW 268 6 20 44 88 125 160
红肉RR 340 7 24 58 103 153 203
总计Total 466 7 25 63 117 191 261

Table 3

Diversity indexes of endophytic microorganisms in stems between H. undatus and H. polyrhizus cultivars"

处理
Treatment		 香农指数
Shannon index		 辛普森指数
Simpson index		 Ace指数
Ace index		 Chao1指数
Chao1 index		 覆盖范围
Coverage (%)
内生细菌
Endophytic bacteria		 白肉RW 3.71±0.46a 0.07±0.032a 410.36±56.91a 403.63±63.72a 0.99
红肉RR 3.82±0.58a 0.06±0.032a 408.99±57.14a 404.74±63.53a 0.99
内生真菌
Endophytic fungi		 白肉RW 1.84±0.93a 0.38±0.301a 154.52±49.35a 156.17±48.77a 0.99
红肉RR 2.65±0.14a 0.13±0.008a 171.38±40.97a 175.96±38.94a 0.99

Fig. 1

Relative abundance of dominant endophytic bacteria in stems between H. undatus (A) and H. polyrhizus (B) at phylum level"

Fig. 2

Relative abundance of dominant endophytic bacteria in stems between H. undatus (A) and H. polyrhizus (B) at genus level"

Fig. 3

Relative abundance of dominant endophytic fungi in stems between H. undatus (A) and H. polyrhizus (B) at phylum level"

Fig. 4

Relative abundance of dominant endophytic fungi in stems between H. undatus (A) and H. polyrhizus (B) at genus level"

Fig. 5

Multi-level species hierarchical tree diagram (A) and LDA discriminant histogram (B) for LEfSe analysis of endophytic bacteria in stems between H. undatus and H. polyrhizus cultivars"

Fig. 6

Multi-level species hierarchical tree diagram (A) and LDA discriminant histogram (B) for LEfSe analysis of endophytic fungi in stems between H. undatus and H. polyrhizus cultivars"

Fig. 7

Venn diagram of endophytic bacteria (A) and fungi (B) in stems between H. undatus and H. polyrhizus cultivars at OTU level"

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