Scientia Agricultura Sinica ›› 2004, Vol. 37 ›› Issue (10): 1580-1584 .doi: 10.3864/j.issn.0578-1752.041031

• RESEARCH NOTES • Previous Articles    

Isolation of NBS-LRR Class Resistance Gene Analogs from Stigma cDNA of Pomelo (Citrus grandis cv. guanxi)

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  1. 中国农业大学植物营养系
  • Received:2002-11-27 Revised:2004-03-25 Online:2004-10-20 Published:2004-10-20

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Abstract: Degenerate oligonucleotide primers based on conserved motifs in the nucleotide binding site (NBS) region of known resistance proteins were used to amplify sequences from stigma cDNA of pomelo (Citrus grandis cv. guanxi) at anthesis by polymerase chain reaction (PCR). A bright band of about 500 bp was obtained. After cloning, 12 classes of clones were selected for sequencing. Searching in the GenBank database using BLAST revealed that 10 classes of sequences showed high level of amino acid similarity to several cloned NBS-LRR class plant resistance genes and many RGAs from other plant species. These pomelo resistance gene analogs (RGAs) are all　expressed sequences because of coming from cDNA. Therefore, they are valuable probes for screening candidate disease genes in pomelo.

Key words: Pomelo, R gene, NBS-LRR class R gene analogs

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