Scientia Agricultura Sinica ›› 2005, Vol. 38 ›› Issue (11): 2233-2239 .

• PLANT PROTECTION • Previous Articles     Next Articles

Cloning and Sequence Analysis of a cDNA Encoding Chemosensory Protein in Helicoverpa armigera

,,   

  1. 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室， 北京
  • Received:2005-01-24 Revised:1900-01-01 Online:2005-11-10 Published:2005-11-10

PDF

851

Cited

6

Abstract: A full-length cDNA, CSPHarm, was cloned from antennae of Helicoverpa armigera. The open reading-fragment of CSPHarm was 381bp, encoding 127 amino acid residues. This deduced amino acid sequence shared some common structural features with chemosensory proteins in insects. It also shared high identity with chemosensory proteins from other insects. Therefore, CSPHarm was a cDNA encoding chemosensory protein of H.armigera. CSPHarm displayed a strong hydrophilicity all along the molecular, except for the hydrophobic domain between residues 20-30, which was likely binding domain of hydrophobic compounds. Semi-quantitative RT-PCR analysis showed that CSPHarm was highly expressed in head, thorax, abdomen, leg, wing and antenna of H. armigera. No obrious difference at expressed level of CSPHarm among these tissues. CSPHarm was also expressed in egg, larva, pupae and adult and relatively low expressed in egg and highly expressed in adult.

Key words: Helicoverpa armigera, Chemosensory protein, Gene cloning

[1] GU LiDan,LIU Yang,LI FangXiang,CHENG WeiNing. Cloning of Small Heat Shock Protein Gene Hsp21.9 in Sitodiplosis mosellana and Its Expression Characteristics During Diapause and Under Temperature Stresses [J]. Scientia Agricultura Sinica, 2023, 56(1): 79-89.
[2] LI YuZe,ZHU JiaWei,LIN Wei,LAN MoYing,XIA LiMing,ZHANG YiLi,LUO Cong,HUANG Gui Xiang,HE XinHua. Cloning and Interaction Protein Screening of RHF2A Gene from Xiangshui Lemon [J]. Scientia Agricultura Sinica, 2022, 55(24): 4912-4926.
[3] QU Cheng,WANG Ran,LI FengQi,LUO Chen. Cloning and Expression Profiling of Gustatory Receptor Genes BtabGR1 and BtabGR2 in Bemisia tabaci [J]. Scientia Agricultura Sinica, 2022, 55(13): 2552-2561.
[4] ZHANG Li,ZHANG Nan,JIANG HuQiang,WU Fan,LI HongLiang. Molecular Cloning and Expression Pattern Analysis of NPC2 Gene Family of Apis cerana cerana [J]. Scientia Agricultura Sinica, 2022, 55(12): 2461-2471.
[5] XiaoHe LIU,GuiSheng QIU,ZhaoGuo TONG,HuaiJiang ZHANG,WenTao YAN,Qiang YUE,LiNa SUN. Ligands Binding Characteristics of Chemosensory Protein CsasCSP16 of Carposina sasakii [J]. Scientia Agricultura Sinica, 2021, 54(5): 945-958.
[6] ZHANG Lu,ZONG YaQi,XU WeiHua,HAN Lei,SUN ZhenYu,CHEN ZhaoHui,CHEN SongLi,ZHANG Kai,CHENG JieShan,TANG MeiLing,ZHANG HongXia,SONG ZhiZhong. Identification, Cloning, and Expression Characteristics Analysis of Fe-S Cluster Assembly Genes in Grape [J]. Scientia Agricultura Sinica, 2021, 54(23): 5068-5082.
[7] TAN YongAn,JIANG YiPing,ZHAO Jing,XIAO LiuBin. Expression Profile of G Protein-Coupled Receptor Kinase 2 Gene (AlGRK2) and Its Function in the Development of Apolygus lucorum [J]. Scientia Agricultura Sinica, 2021, 54(22): 4813-4825.
[8] WANG Na,ZHAO ZiBo,GAO Qiong,HE ShouPu,MA ChenHui,PENG Zhen,DU XiongMing. Cloning and Functional Analysis of Salt Stress Response Gene GhPEAMT1 in Upland Cotton [J]. Scientia Agricultura Sinica, 2021, 54(2): 248-260.
[9] TAN YongAn,ZHAO XuDong,JIANG YiPing,ZHAO Jing,XIAO LiuBin,HAO DeJun. Cloning, Preparation of Antibody and Response Induced by 20-Hydroxyecdysone of Target of Rapamycin in Apolygus lucorum [J]. Scientia Agricultura Sinica, 2021, 54(10): 2118-2131.
[10] KunNeng ZHOU,JiaFa XIA,Peng YUN,YuanLei WANG,TingChen MA,CaiJuan ZHANG,ZeFu LI. Transcriptome Research of Erect and Short Panicle Mutant esp in Rice [J]. Scientia Agricultura Sinica, 2020, 53(6): 1081-1094.
[11] SHEN JingYuan,TANG MeiLing,YANG QingShan,GAO YaChao,LIU WanHao,CHENG JieShan,ZHANG HongXia,SONG ZhiZhong. Cloning, Expression and Electrophysiological Function Analysis of Potassium Channel Gene VviSKOR in Grape [J]. Scientia Agricultura Sinica, 2020, 53(15): 3158-3168.
[12] CHEN Lin,WEI JiZhen,LIU Chen,NIU LinLin,ZHANG CaiHong,LIANG GeMei. Effect of Midgut Specific Binding Protein ABCC1 on Cry1Ac Toxicity Against Helicoverpa armigera [J]. Scientia Agricultura Sinica, 2019, 52(19): 3337-3345.
[13] JIANG MengTing,ZHU Ning,GONG HongYong,HOU YingJun,YU XinYi,QU ShenChun. Cloning and Function Analysis of Gibberellin Insensitive DkGAI2 Gene in Nantongxiaofangshi (Diospyros kaki Linn. cv. nantongxiaofangshi) [J]. Scientia Agricultura Sinica, 2019, 52(19): 3417-3429.
[14] ZHAO Jie, REN SuWei, LIU Ning, AI XinYu, MA Ji, LIU XiaoNing. Cloning and Expression of Phosphatidylethanolamine Binding Protein in Helicoverpa armigera [J]. Scientia Agricultura Sinica, 2018, 51(8): 1493-1503.
[15] LIU Chao, WANG LingLi, WU Di, DANG JiangBo, SHANG Wei, GUO QiGao, LIANG GuoLu. Molecular Cloning of Leaf Developmental Gene EjGRF5, Its Promoter and Expression Analysis in Different Ploidy Loquat (Eriobotrya japonica (Thunb.) Lindl.) [J]. Scientia Agricultura Sinica, 2018, 51(8): 1598-1606.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd