Scientia Agricultura Sinica ›› 2005, Vol. 38 ›› Issue (11): 2233-2239 .doi: 10.3864/j.issn.0578-1752.at-2005-5122

• PLANT PROTECTION • Previous Articles     Next Articles

Cloning and Sequence Analysis of a cDNA Encoding Chemosensory Protein in Helicoverpa armigera

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  1. 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室， 北京
  • Received:2005-01-24 Revised:1900-01-01 Online:2005-11-10 Published:2005-11-10

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Abstract: A full-length cDNA, CSPHarm, was cloned from antennae of Helicoverpa armigera. The open reading-fragment of CSPHarm was 381bp, encoding 127 amino acid residues. This deduced amino acid sequence shared some common structural features with chemosensory proteins in insects. It also shared high identity with chemosensory proteins from other insects. Therefore, CSPHarm was a cDNA encoding chemosensory protein of H.armigera. CSPHarm displayed a strong hydrophilicity all along the molecular, except for the hydrophobic domain between residues 20-30, which was likely binding domain of hydrophobic compounds. Semi-quantitative RT-PCR analysis showed that CSPHarm was highly expressed in head, thorax, abdomen, leg, wing and antenna of H. armigera. No obrious difference at expressed level of CSPHarm among these tissues. CSPHarm was also expressed in egg, larva, pupae and adult and relatively low expressed in egg and highly expressed in adult.

Key words: Helicoverpa armigera, Chemosensory protein, Gene cloning

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