Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (22): 4600-4609.doi: 10.3864/j.issn.0578-1752.2011.22.006

• PLANT PROTECTION • Previous Articles     Next Articles

Effects of Tobacco Plants Infested by Bemisia tabaci (Gennadius) B-biotype on the Development and Reproduction of Spodoptera litura (Fabricius) and Related Mechanisms

 WANG  Hong-Tao, XUE  Ming, LI  Qing-Liang, ZHANG  Xiao, ZHOU  Fang-Yuan   

  1. 1.山东农业大学植物保护学院，山东泰安 271018
    2.山东省烟台市农业科学研究院植物保护研究所，山东烟台 265500
  • Received:2011-03-08 Online:2011-11-15 Published:2011-05-25

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Abstract: 【Objective】 The objective of this study is to define defense response of tobacco plants to Spodoptera litura induced by Bemisia tabaci B biotype and their spatial effect, and to explore its role in interspecific competition between B. tabaci and S. litura and its possible mechanism. 【Method】 The effects of leaves in different positions of tobacco preinfested by B. tabaci B-biotype on growth, development and fecundity of S. litura were investigated by feeding method and the change of related defense enzymes was analyzed using biochemical method in laboratory. 【Result】 The results showed that tobacco plants preinfested by B. tabaci B-biotype feeding had a negative effect on growth, development and fecundity of S. litura, and different leaf positions of treated plants reacted differently, the sequence was the whitefly-infested leaves＞the middle leaves＞the white-vein leaves. There were significant differences between treatment and its control. Trypsin protease inhibitor (TPI) content, β-1,3-glucanase activity and chitinase activity in the whitefly-infested leaves, the middle leaves and the white-vein leaves of the treated tobacco plants all increased significantly. The unexpanded leaves showed no significant effect on both the growth, development and fecundity of S. litura and the change of related defense enzymes. 【Conclusion】 Tobacco preinfested by B. tabaci B-biotype had a negative effect on growth, development and fecundity of S. litura. Furthermore, this effect was systemic and could be transported through the plant. Infestation by B. tabaci B-biotype in tobacco plants led to the higher induction of TPI, β-1, 3-glucanase and chitinase, and they may have a correlation with the negative effect on S. litura. The defense responses of tobacco plants induced by B. tabaci B-biotype feeding were considered to play an important role in the host defense against S. litura.

Key words: Bemisia tabaci B-biotype, tobacco, Spodoptera litura, growth and development, reproduction, spatial effect, defense enzyme

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