Scientia Agricultura Sinica ›› 2023, Vol. 56 ›› Issue (16): 3077-3087.doi: 10.3864/j.issn.0578-1752.2023.16.003

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

COXⅡ Functional Analysis in Tobacco sua-CMS Line

WANG Jin1,2(), LIU YanFang1, LIU WenWen3, WANG YaQi1,2, SONG ShiYang1,2, ZHANG XingZi1,2, LI FengXia1()   

  1. 1 Institute of Tobbcco Research of Chinese Academy of Agricultural Sciences, Qingdao 266100, Shandong
    2 Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081
    3 Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266100, Shandong
  • Received:2023-02-16 Accepted:2023-05-26 Online:2023-08-16 Published:2023-08-18

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Abstract:

【Objective】Cytoplasmic male sterility (CMS) is controlled by the cytoplasmic genome, mainly the mitochondrial genome, which plays an important role in breeding and production of hybrids. Sterile lines and hybrids are the main types of tobacco cultivation in China, accounting for more than 80% of the total tobacco cultivation area. The sterile type of tobacco in China is sua-CMS, which is also the only sterile type of tobacco production, but the control gene and sterility mechanism of sua-CMS are not clear. COXⅡ is the second subunit of cytochrome oxidase (COX), encoded by the mitochondrial genome. Nucleotide base variants occur at the terminal of the COXⅡ in sua-CMS lines, which resulted in the replacement of the C-terminal of COXⅡ Thr257 by Ser. The function analysis of COXⅡ in sua-CMS tobacco provides a foundation for the elucidation of the molecular mechanism of sterility in tobacco.【Method】In this study, four sets of sua-CMS lines and fertile controls were used as materials, and RNA blot hybridization and qRT-PCR methods were used to analyze the transcript and gene expression of COXⅡ between sua-CMS lines and fertile controls, and Western blot and COX enzyme activity experiments analyzed COX protein expression and COX enzyme activity, and ATP content were measured. The COXⅡ of fertile tobacco was overexpressed in sua-CMS line, and the effects of COXⅡ overexpression on COXⅡ expression, COX enzyme activity, ATP content and stamens development of tobacco were studied.【Result】COXⅡ multi-sequence alignment showed that Thr257 at C-terminal was highly conserved between species. Expression analysis showed that the COXⅡ transcript in sua-CMS line was significantly lower than that of the fertile control, and quantitative expression of four sets of sua-CMS lines and fertile controls showed that the expression amount of COXⅡ in sua-CMS lines was only 0.3%-0.4% of the fertile controls, the COX protein abundances and enzyme activity were significantly lower than that of the fertile control, and the ATP content of sua-CMS line was 52% of that of the fertile control. When COXⅡ was overexpressed in sua-CMS line, the expression levels of COXⅡ in transgenic plants increased by up to 171 times, the COX enzyme activity increased by 70%, the ATP content increased by 90%. However, all indicators including COXⅡ expression levels, COX enzyme activity and ATP content in transgenic plants were lower than those of fertile tobacco. We also observed significant changes in anther morphology in transgenic plants, and the differentiation of the anther wall appeared, but there was no pollen grains in the anthers.【Conclusion】This study shows that the mutant COXⅡ of sua-CMS line affects COX function, which is a main reason of anther energy deficiency, and it is also an important factor affecting the development of anthers.

Key words: tobacco, cytoplasmic male sterility, COXⅡ, functional identification

Table 1

sua-CMS tobacco sterile lines and fertile controls"

材料分组
Material set		 材料名称
Material name		 类型
Type		 育性
Fertility
Ⅰ MS中烟100 MS ZY100 sua-CMS不育系sua-CMS line 不育Sterile
中烟100 ZY100 保持系Maintainer line 可育Fertile
Ⅱ MS云烟87 MS YY87 sua-CMS不育系sua-CMS line 不育Sterile
云烟87 YY87 保持系Maintainer line 可育Fertile
Ⅲ MS中烟300 MS ZY300 sua-CMS不育系sua-CMS line 不育Sterile
中烟300 ZY300 保持系Maintainer line 可育Fertile
Ⅳ 中烟202 ZY202 sua-CMS杂交种sua-CMS hybrid 不育Sterile
中烟98 ZY98 可育亲本Fertile parent 可育Fertile

Table 2

The primer sequences in this study"

引物
Primer		 正向引物
F-Primer (5′-3′)		 反向引物
R-Primer (5′-3′)
N-COXⅡ ATGATTGTTCTAGAATGGCTATTC TTAAGCTTCCCCGGTTTGTG
N-Actin CTATTCTCCGCTTTGGACTTGGCA AGGACCTCAGGACAACGGAAACG
Actin CAAGGAAATCACCGCTTTGG AAGGGATGCGAGGATGGA
qCOXⅡ TGTGATGCTGTACCTGGTCG TAAGCTTCCCCGGTTTGTGG
AP3 TATGACCATGATTACGAATTCCAGTAACTGTGGCCAACTTAGTTTTG GACGTAGTGAAAGCATATTCTTCTCTCTTTG
MTS CAAAGAGAGAAGAATATGCTTTCACTACGT CCGAATACTCATAAGACCCTCTTTAGCACC
COXⅡ GGTGCTAAAGAGGGTCTTATGAGTATTCGG ATTCTGGTGTGTGCGCAATG
P ATTCTGGTGTGTGCGCAATG

Fig. 1

COXⅡ multiple sequence alignment"

Fig. 2

Analysis of COXⅡ gene expression A: RNA gel-blotting of COXII in floral buds of sua-CMS line and fertile control; B: Quantitative expression of COXII in floral buds of four sets of sua-CMS tobacco and fertile control; C: Quantitative expression of COXII in smal buds of four sets of sua-CMS tobacco and fertile control; D: Expression analysis of COXII in different organs and buds at different developmental stages"

Fig. 3

COX protein expression and enzyme activity of sterile line and maintainer line at different stages of bud A: Western blotting analysis of COX of MS ZY100 and ZY100; B: Analysis of enzymatic activity of complex Ⅳ in floral bud and small bud of different fertility; C: Analysis of ATP content in floral bud and small bud of different fertility. *significants P<0.05, ***significants P<0.001"

Fig. 4

Construction of COXⅡ overexpression vector A: A schematic diagram of the chimeric gene AP3::MTS-COXII; B: Fragment amplification in vector construction"

Fig. 5

Analysis of COXⅡ expression of transgenic materials"

Fig. 6

COX enzyme activity and ATP content of transgenic plants A: COX enzyme activity of transgenic plants; B: ATP content of transgenic plants"

Fig. 7

Anthers development of transgenic plants A: Anther morphology of MS ZY100; B: Anther structure of MS ZY100; C: Pollen fluorescence staining of ZY100; D: Anther morphology of OE-4; E: Anther structure of OE-4; F: Pollen fluorescence staining of OE-4"

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