Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (16): 3297-3311.doi: 10.3864/j.issn.0578-1752.2011.16.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Comparative Analysis of Genetic Structure and Differentiation of Guangdong and Guangxi Tea Germplasms Based on EST-SSR Markers

QIAO  Xiao-Yan, QIAO  Ting-Ting, ZHOU  Yan-Hua, JIN  Ji-Qiang, MA  Chun-Lei, YAO  Ming-Zhe, CHEN  Liang   

  1. 1. 广东省农业科学院茶叶研究所
    2. 中国农业科学院茶叶研究所国家茶树改良中心
  • Received:2011-01-04 Revised:2011-03-10 Online:2011-08-15 Published:2011-03-16

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Abstract: 【Objective】 The well understanding of genetic diversity, structure and differentiation of tea germplasms is important for their effective conservation and reasonable utilization. 【Method】 One hundred and nine core EST-SSR markers were selected and used to analyze the genetic diversity and differentiation of 105 tea accessions from Guangdong and Guangxi provinces. Molecular variance (AMOVA) analysis of inter-groups, inter-populations and individuals within populations was conducted, and then the model-based genetic structure was constructed. 【Result】 A total of 435 alleles were identified with an average of 3.99 alleles per marker (NA). The average of effective number of alleles (NE), Nei's gene diversity (H), observed heterozygosity (Ho), expected heterozygosity (He) and polymorphism information content (PIC) was 2.12, 0.32, 0.59, 0.46 and 0.56, respectively. Guangdong tea germplasms showed lower values of the six indexes mentioned above, compared to Guangxitea. Guangdong Baimaocha (Camellia sinensis var. pubilimba Chang) had lower NA and H values and similar NE, He, Ho and PIC values compared with Guangdong tea (C. sinensis (L.) O. Kuntze). While Guangxi Baimaocha had higher NA value, lower NE, He and H values than Guangxi tea. The values of Fis, Fit and Fst were low among Guangxi and Guangdong Baimaocha and tea populations with strong gene flow. The same results were found between Baimaocha and tea. There were weak genetic differentiation (0.04) and gene flow (6.26) within Baimaocha population. Molecular variance (AMOVA) analysis showed that there was a relatively low level (3.09％) of genetic variation among Guangdong and Guangxi tea germplasms, that within the populations was 94.69%. The population structure analysis could divide all the 105 accessions into 3 groups. Six developed cultivars were grouped into groupⅠ; group Ⅱ consisted of 43 accessions from Guangdong and 16 from Guangxi. The remaining 36 accessions from Guangxi and 4 from Guangdong were assigned into group III.【Conclusion】The genetic diversity of Guangdong tea germplasms was lower than that of Guangxi. Genetic differentiation was insignificant between populations with strong gene flow. Guangdong Baimaocha had much more abundant genetic diversity than Guangdong tea, and genetic differentiation was weak with frequent genetic exchange. Guangxi Baimaocha showed richer genetic diversity than Guangdong Baimaocha, with relatively high genetic divergence and weak gene flow. AMOVA showed that the variation level within Guangdong and Guangxi populations was absolutely higher than among populations.

Key words: Tea germplasm, Baimaocha, genetic diversity, genetic structure, genetic differentiation

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