Scientia Agricultura Sinica ›› 2007, Vol. 40 ›› Issue (8): 1774-1789 .

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Determination of DNA methylation content both in the blood and muscle tissue of pigs and significant difference analysis

  

  1. 华中农业大学农业部猪遗传育种重点开放实验室
  • Received:2006-04-30 Revised:2006-09-07 Online:2007-08-10 Published:2007-08-10

PDF

932

Abstract: Abstract:【Objective】In this experiment, parental lines Large White、Landrace、Meishan and their hybrids Large White × Landrace、Landrace × Large White、Large White×Meishan、Meishan×Large White were used as the source of DNA samples for the determination of DNA methylation content both in the blood and muscle tissue of pigs, at the same time, the significant difference about DNA methylation content between parental lines and their hybrids, base on different hybrid systems and different tissues, were analyzed.【Method】High performance liquild chromatography, HPLC.【Result】The average DNA methylation content in the 163 samples of muscle tissue is 16.92%, while the average DNA methylation content in the 182 samples of blood is 6.49%, the difference between which is especially prominent (p<0.01). In the same tissue but different hybrid system, hybrids appear different. Similarly, in the same hybrid system but different tissue, hybrids are different too. 【Conclusion】The increase of DNA methylation percentage in hybrids may result in more silence expression of genes which are disbenificial to development. The hybrids methylation percentages differ in hybrid combination and tissue specificity.

Key words: HPLC, DNA methylation, pig

[1] TAN XianMing,ZHANG JiaWei,WANG ZhongLin,CHEN JunXu,YANG Feng,YANG WenYu. Prediction of Maize Yield in Relay Strip Intercropping Under Different Water and Nitrogen Conditions Based on PLS [J]. Scientia Agricultura Sinica, 2022, 55(6): 1127-1138.
[2] CHEN XueSen, YIN HuaLin, WANG Nan, ZHANG Min, JIANG ShengHui, XU Juan, MAO ZhiQuan, ZHANG ZongYing, WANG ZhiGang, JIANG ZhaoTao, XU YueHua, LI JianMing. Interpretation of the Case of Bud Sports Selection to Promote the High-Quality and Efficient Development of the World’s Apple and Citrus Industry [J]. Scientia Agricultura Sinica, 2022, 55(4): 755-768.
[3] MingJie XING,XianHong GU,XiaoHong WANG,Yue HAO. Effects of IL-15 Overexpression on Myoblast Differentiation of Porcine Skeletal Muscle Cells [J]. Scientia Agricultura Sinica, 2022, 55(18): 3652-3663.
[4] YANG ChangPei,WANG NaiXiu,WANG Kai,HUANG ZiQing,LIN HaiLan,ZHANG Li,ZHANG Chen,FENG LuQiu,GAN Ling. Effects and Mechanisms of Exogenous GABA Against Oxidative Stress in Piglets [J]. Scientia Agricultura Sinica, 2022, 55(17): 3437-3449.
[5] DENG FuLi,SHEN Dan,ZHONG RuQing,ZHANG ShunFen,LI Tao,SUN ShuDong,CHEN Liang,ZHANG HongFu. Non-Starch Polysaccharide Enzymes Cocktail of Corn-Miscellaneous Meal-Based Diet Optimization by In Vitro Method and Its Effects on Intestinal Microbiome in Finishing Pigs [J]. Scientia Agricultura Sinica, 2022, 55(16): 3242-3255.
[6] JIN MengJiao,LIU Bo,WANG KangKang,ZHANG GuangZhong,QIAN WanQiang,WAN FangHao. Light Energy Utilization and Response of Chlorophyll Synthesis Under Different Light Intensities in Mikania micrantha [J]. Scientia Agricultura Sinica, 2022, 55(12): 2347-2359.
[7] HAN Xiao, YANG HangYu, CHEN WeiKai, WANG Jun, HE Fei. Effects of Different Rootstocks on Flavonoids of Vitis vinifera L. cv. Tannat Grape Fruits [J]. Scientia Agricultura Sinica, 2022, 55(10): 2013-2025.
[8] HU RongRong,DING ShiJie,GUO Yun,ZHU HaoZhe,CHEN YiChun,LIU Zheng,DING Xi,TANG ChangBo,ZHOU GuangHong. Effects of Trolox on Proliferation and Differentiation of Pig Muscle Stem Cells [J]. Scientia Agricultura Sinica, 2021, 54(24): 5290-5301.
[9] TANG ZhenShuang,YIN Dong,YIN LiLin,MA YunLong,XIANG Tao,ZHU MengJin,YU Mei,LIU XiaoLei,LI XinYun,QIU XiaoTian,ZHAO ShuHong. To Evaluate the “Two-Step” Genomic Selection Strategy in Pig by Simulation [J]. Scientia Agricultura Sinica, 2021, 54(21): 4677-4684.
[10] ZHANG DanDan,XU TengTeng,GAO Di,QI Xin,NING Wei,RU ZhenYuan,ZHANG XiangDong,GUO TengLong,SHENTU LuYan,YU Tong,MA YangYang,LI YunSheng,ZHANG YunHai,CAO ZuBing. Transcription Factor TEAD4 Regulates Early Embryonic Development in Pigs [J]. Scientia Agricultura Sinica, 2021, 54(20): 4456-4465.
[11] SHI Jiang,WANG JiaTong,PENG QunHua,LÜ Haipeng,BALDERMANN Susanne,LIN Zhi. Changes in Lipid-Soluble Pigments in Fresh Tea Leaves Treated by Methyl Jasmonate and During Postharvest Oolong Tea Manufacturing [J]. Scientia Agricultura Sinica, 2021, 54(18): 3984-3997.
[12] DU Xing,ZENG Qiang,LIU Lu,LI QiQi,YANG Liu,PAN ZengXiang,LI QiFa. Identification of the Core Promoter of Linc-NORFA and Its Transcriptional Regulation in Erhualian Pig [J]. Scientia Agricultura Sinica, 2021, 54(15): 3331-3342.
[13] YU ZhengWang,ZHOU ZhongXin. Functions of Antibacterial and Inducing Defense Peptide Expression of Medium-Chain Fatty Acid and Its Application in Piglet Feeds [J]. Scientia Agricultura Sinica, 2021, 54(13): 2895-2905.
[14] QIN BenYuan,YANG Yang,ZHANG YanWei,LIU Min,ZHANG WanFeng,WANG HaiZhen,WU YiQi,ZHANG XueLian,CAI ChunBo,GAO PengFei,GUO XiaoHong,LI BuGao,CAO GuoQing. Isolation, Culture, Identification and Biological Characteristics of Pig Skeletal Muscle Satellite Cells [J]. Scientia Agricultura Sinica, 2020, 53(8): 1664-1676.
[15] HuiMin HU,XueFeng PAN,Heng YANG,Chen CHEN,YinJi CHEN. Wheat Gluten, Gliadins and Glutenin Content Changes During Germination Based on the Methods of R5 ELISA and RP-HPLC [J]. Scientia Agricultura Sinica, 2020, 53(6): 1247-1255.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd