Scientia Agricultura Sinica ›› 2007, Vol. 40 ›› Issue (8): 1774-1789 .doi: 10.3864/j.issn.0578-1752.at-2006-7528

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Determination of DNA methylation content both in the blood and muscle tissue of pigs and significant difference analysis

  

  1. 华中农业大学农业部猪遗传育种重点开放实验室
  • Received:2006-04-30 Revised:2006-09-07 Online:2007-08-10 Published:2007-08-10

PDF

953

Abstract: Abstract:【Objective】In this experiment, parental lines Large White、Landrace、Meishan and their hybrids Large White × Landrace、Landrace × Large White、Large White×Meishan、Meishan×Large White were used as the source of DNA samples for the determination of DNA methylation content both in the blood and muscle tissue of pigs, at the same time, the significant difference about DNA methylation content between parental lines and their hybrids, base on different hybrid systems and different tissues, were analyzed.【Method】High performance liquild chromatography, HPLC.【Result】The average DNA methylation content in the 163 samples of muscle tissue is 16.92%, while the average DNA methylation content in the 182 samples of blood is 6.49%, the difference between which is especially prominent (p<0.01). In the same tissue but different hybrid system, hybrids appear different. Similarly, in the same hybrid system but different tissue, hybrids are different too. 【Conclusion】The increase of DNA methylation percentage in hybrids may result in more silence expression of genes which are disbenificial to development. The hybrids methylation percentages differ in hybrid combination and tissue specificity.

Key words: HPLC, DNA methylation, pig

[1] ZHOU Qi, ZHANG ShiHao, ZHANG Liang, PAN Yu, ZHANG LiJuan, TU Zhi, PAN HongMei, LONG Xi. Prediction of the Potential Habitat Suitability of Luopanshan Pigs in Chongqing Based on the Optimized MaxEnt Model [J]. Scientia Agricultura Sinica, 2026, 59(1): 205-219.
[2] WANG JiYing, LI JingXuan, WANG YanPing, GUO JianFeng, LIN HaiChao, ZHAO XueYan. Weighted Gene Co-Expression Network Analysis Reveals Potential Candidate Genes Affecting Fat Deposition in Pigs [J]. Scientia Agricultura Sinica, 2025, 58(9): 1845-1855.
[3] JIN YiDan, HE NiQing, CHENG ZhaoPing, LIN ShaoJun, HUANG FengHuang, BAI KangCheng, ZHANG Tao, WANG WenXiao, YU MinXiang, YANG DeWei. Screening and Identification of Pigm-1 Interaction Proteins for Disease Resistance of Rice Blast [J]. Scientia Agricultura Sinica, 2025, 58(6): 1043-1051.
[4] LIU Jing, ZHAO ShiLin, YANG XiaoTing, WEI YiXuan, LI JiaPeng, ZHAO Yan. Study on the Origin Traceability of Beijing Black Pig Based on Stable Isotope Technology [J]. Scientia Agricultura Sinica, 2025, 58(23): 5071-5080.
[5] LUO Qin, CHEN XieYong, XU YuYing, WEI Hang, HUANG Biao, YAO QingHua, YE NaiXing, ZHENG DeYong, YAN MingJuan. Characterization of Non-Volatile Metabolites of White Peony Tea Make of Camellia sinensis Fu’an-dabaicha from Different Origins [J]. Scientia Agricultura Sinica, 2025, 58(22): 4757-4770.
[6] GAO Rong, LI HengYu, CHEN LiJuan, MA HuiLing. Physiological Effects of 5-AzaC on Alleviating Salt‑Alkali Stress in Alfalfa and Its Impact on the Expression of DNA Methylation Enzyme Genes [J]. Scientia Agricultura Sinica, 2025, 58(21): 4482-4496.
[7] GAO XiaoPing, PAN HongMei, GUO ZongYi, ZHANG JunJie, LIN Yan, ZHANG Liang. Post-Freezing Quality and Targeted Lipidomics Analysis of Rongchang Pig Spermatozoa with Different Freezing Tolerance [J]. Scientia Agricultura Sinica, 2025, 58(2): 387-400.
[8] ZHANG HuaPeng, ZHANG QingZe, HE Fan, QI MengFan, FU BinBin, LI QingChun, LI MengXun, MA LiPeng, LIU Yi, HUANG Tao. Cloning and Identification of Differentially Expressed lncRNAs in Follicles of Meishan Pigs and Duroc Pigs with Their Correlation Analysis with miRNAs [J]. Scientia Agricultura Sinica, 2024, 57(9): 1807-1819.
[9] ZHAO ZhenJian, WANG Kai, CHEN Dong, SHEN Qi, YU Yang, CUI ShengDi, WANG JunGe, CHEN ZiYang, YU ShiXin, CHEN JiaMiao, WANG XiangFeng, TANG GuoQing. Integrated Aanalysis of Genome and DNA Methylation for Screening Key Genes Related to Pork Quality Traits [J]. Scientia Agricultura Sinica, 2024, 57(7): 1394-1406.
[10] GAO ChenXi, HAO LuYang, HU Yue, LI YongXiang, ZHANG DengFeng, LI ChunHui, SONG YanChun, SHI YunSu, WANG TianYu, LI Yu, LIU XuYang. Analysis of Transposable Element Associated Epigenetic Regulation under Drought in Maize [J]. Scientia Agricultura Sinica, 2024, 57(6): 1034-1048.
[11] LIU ZhuoLin, LIU HongYun. The Potential and Mechanisms of Apigenin to Relieve Heat Stress and Hypoxia in Dairy Cows Based on Network Pharmacology and Molecular Docking [J]. Scientia Agricultura Sinica, 2024, 57(5): 1010-1022.
[12] SU XiaoYu, TAN ZhengWei, LI ChunMing, LI Lei, LU DanDan, YU YongLiang, DONG Wei, AN SuFang, YANG Qing, SUN Yao, XU LanJie, YANG HongQi, LIANG HuiZhen. Analysis of Genome-Wide Methylation Differences and Associated Gene Expression of Sesame Varieties Under High Temperature Stress [J]. Scientia Agricultura Sinica, 2024, 57(24): 4825-4838.
[13] MA YuHe, PU YuanYuan, WANG JinXiong, WU JunYan, YANG Gang, ZHAO CaiXia, MA Li, LIU LiJun, WANG WangTian, MIAO ChunQing, GUAN ZhouBo, FAN TingTing, WANG XingRong, MA Rui, LIAN YinTao, SUN WanCang. Analysis of Glucosinolate Content and Component in Brassica rapa L. [J]. Scientia Agricultura Sinica, 2024, 57(21): 4308-4327.
[14] ZHOU YuanQing, DONG HongMin, ZHU ZhiPing, WANG Yue, LI NanXi. Review on Carbon Footprint Assessment of Pig Farming System [J]. Scientia Agricultura Sinica, 2024, 57(2): 379-389.
[15] CAI RuiJie, CHU YiXin, SHI XinE, JIN JianJun, YANG GongShe. Dietary Addition of Cordyceps Militaris Can Alleviate Lipopolysaccharide- Induced Liver Damage and Skeletal Muscle Protein Degradation in Early Weaning Piglets [J]. Scientia Agricultura Sinica, 2024, 57(12): 2467-2482.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd