Abstract: Abstract: 【Aim】 To test the feasibility of preparing acellular matrices of sheep carotids with enzyme-eradicator method, explore a new method, prepare good acellular matrices of sheep carotids, and provide scaffolds for constructing tissue-engineering blood vessels. 【Method】 Repeated freezing-thaw ─ 1%Triton X-100 + PMSF method and 0.25% trypsin ─ 1%Triton X-100 method were used for preparing acellular matrices of sheep carotids. The Specimens were observed with eyes, optical microscope and scanning electron microscope, measured for mechanic properties and compared.【Result】The repeated freezing-thaw ─ 1%Triton X-100 + PMSF method not only entirely removed the cells of samples and maintained the normal structure of matrix fibre in samples, but also the acellular matrices prepared with it maintain good shape and mechanical properties. Though the 0.25% trypsin ─ 1%Triton X-100 method entirely removed the cells of samples, but the acellular matrices prepared with it did not maintain shape and strain, with dent. 【Conclusion】 Compared with the 0.25% trypsin ─ 1%Triton X-100 method, the repeated freezing-thaw ─ 1%Triton X-100 + PMSF method is a better and new method for preparing acellular matrices of sheep carotids

Key words: Carotids, Acellular matrix, Sheep