Scientia Agricultura Sinica ›› 2021, Vol. 54 ›› Issue (2): 392-399.doi: 10.3864/j.issn.0578-1752.2021.02.014

• FOOD SCIENCE AND ENGINEERING • Previous Articles     Next Articles

Application of Stable Isotope Technology in the Origin Traceability of Sheep

WANG Qian1,2(),LI Zheng1,3(),ZHAO ShanShan1,QIE MengJie1,ZHANG JiuKai4,WANG MingLin3,GUO Jun2,ZHAO Yan1()   

  1. 1Institute of Agricultural Quality Standards & Testing Technology, Chinese Academy of Agricultural Sciences, Key Laboratory of Agricultural Product Quality and Safety, Ministry of Agriculture and Rural Areas, Beijing 100081
    2College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018
    3College of Food Science and Engineering, Shandong Agricultural University, Taian 271018
    4Chinese Academy of Inspection and Quarantine, Agro-Product Safety Research Center, Beijing 100176
  • Received:2020-05-21 Accepted:2020-09-03 Online:2021-01-16 Published:2021-02-03
  • Contact: Yan ZHAO E-mail:1639828031@qq.com;408209783@qq.com;zhaoyan01@caas.cn

Abstract:

【Objective】By comparing the differences of carbon and nitrogen stable isotopes in different parts of mutton and analyzing the stable isotopes of mutton, whole bone meal, de-fatted bone meal and bone collagen, the technical support was provided for the origin traceability identification of sheep. 【Method】Isotope Ratio Mass Spectrometer (IRMS) was used to determine δ 13C and δ 15N values in different parts of sheep, and δ 13C and δ 15N values in de-fatted mutton, whole bone meal, de-fatted bone meal and bone collagen of the sheep bones from different origins (New Zealand, and Ningxia, Gansu, Anhui, China). ANOVA test, linear discriminant analysis and correlation analysis were carried out on the results to determine the traceability of carbon and nitrogen stable isotope to the origin of sheep. 【Result】The δ 13C values of hind legs and ribs were significantly different from those of chest fork, abdomen and neck (P<0.05), and there was no significant difference in δ15N values among the five parts of hind legs, chest fork, ribs, abdomen and neck (P>0.05). The δ13C and δ 15N values of de-fatted mutton, whole bone meal, de-fatted bone meal and bone collagen from different origins (Ningxia, Gansu, Anhui and New Zealand) were significantly different (P<0.05). De-fatted mutton had the best effect on the origin traceability, with the original discrimination accuracy rate was 84.9% and the cross-validation discrimination accuracy rate was 82.4%. The accuracy rate of distinguishing the origin by whole bone meal and bone collagen was over 65%. The correlation analysis results show that de-fatted mutton, whole bone meal, de-fatted bone meal and bone collagen had extremely significant correlation (P<0.01), and the carbon isotope in bone collagen had the highest correlation with de-fatted bone meal and whole bone meal, with correlation coefficients of 0.903 and 0.866, respectively. 【Conclusion】Stable isotope could effectively identify sheep samples from different origins, and the change trend of stable isotope composition in mutton, whole bone meal, de-fatted bone meal and bone collagen was basically the same, so it was feasible to trace the origin of sheep by using stable isotope technology.

Key words: stable isotope, mutton, sheep bone, origin traceability

Table 1

The δ13C and δ15N values in de-fatted mutton from different parts"

样品名称
Sample name
胸叉
Chest fork (n=12)
腹腩
Abdomen (n=12)
脖子
Neck (n=11)
后腿
Hind legs (n=12)
排骨
Pork ribs (n=12)
δ13C (‰) -11.97±0.35a -12.14±0.38a -12.19±0.40a -12.60±0.45b -13.13±0.55c
δ15N (‰) 3.25±0.43 3.37±0.26 3.15±0.51 3.23±0.43 3.52±0.39

Table 2

δ13C values for samples from different origins"

样品名称
Sample name
脱脂羊肉
De-fatted mutton
全骨粉
Whole bone meal
脱脂骨粉
De-fatted bone meal
骨胶原
Bone collagen
中国宁夏 Ningxia, China (n=20) -13.16±1.17b -16.09±1.89b -12.88±1.44b -13.70±1.94a
中国甘肃 Gansu, China (n=18) -11.10±0.35a -16.05±2.39b -11.59±2.21a -13.07±2.38a
中国安徽 Anhui, China (n=20) -18.90±0.69c -20.59±2.81c -16.90±1.90c -17.62±2.03b
新西兰 New Zealand (n=20) -13.59±0.47b -14.29±0.69a -12.56±0.53ab -12.59±0.52a

Table 3

δ15N values for samples from different origins"

样品名称
Sample name
脱脂羊肉
De-fatted mutton
全骨粉
Whole bone meal
脱脂骨粉
De-fatted bone meal
骨胶原
Bone collagen
中国宁夏 Ningxia, China (n=20) 4.44±0.61b 5.31±1.47a 5.04±1.05a 4.42±1.20a
中国甘肃 Gansu, China (n=18) 3.17±0.42c 4.16±1.12b 4.05±0.62b 3.13±0.71b
中国安徽 Anhui, China (n=20) 5.08±0.44a 5.80±1.05a 5.36±0.74a 4.70±0.71a
新西兰 New Zealand (n=20) 5.39±0.41a 5.39±0.82a 5.36±0.74a 4.29±0.56a

Table 4

Linear discriminant analysis results of δ13C and δ15N values on origins in different samples"

样品
Sample
脱脂羊肉
De-fatted mutton
全骨粉
Whole bone meal
脱脂骨粉
De-fatted bone meal
骨胶原
Bone collagen
中国宁夏 Ningxia, China 60.0% 40.0% 40.0% 40.0%
中国甘肃 Gansu, China 90.0% 80.0% 80.0% 85.0%
中国安徽 Anhui, China 95.0% 90.0% 50.0% 75.0%
新西兰 New Zealand 94.4% 72.2% 88.9% 77.8%
原始判别正确率 Original Discriminant Accuracy 84.9% 70.6% 64.7% 69.5%
中国宁夏 Ningxia, China 50.0% 30.0% 35.0% 35.0%
中国甘肃 Gansu, China 90.0% 75.0% 80.0% 80.0%
中国安徽 Anhui, China 95.0% 90.0% 50.0% 75.0%
新西兰 New Zealand 94.4% 72.2% 88.9% 77.8%
交叉验证判别正确率 Cross-validation discrimination accuracy 82.4% 66.8% 63.5% 67.0%

Fig. 1

Correlation analysis of δ13C values in de-fatted mutton, whole bone meal, de-fatted bone meal and bone collagen"

Fig. 2

Correlation analysis of δ15N values in de-fatted mutton, whole bone meal, de-fatted bone meal and bone collagen"

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