Scientia Agricultura Sinica ›› 2005, Vol. 38 ›› Issue (03): 612-618 .

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Cloning and Sequence Analysis of cDNA Encoding mrjp3 of Apis cerana cerana

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  1. 浙江大学动物科学学院
  • Received:2004-09-29 Revised:1900-01-01 Online:2005-03-10 Published:2005-03-10

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Abstract: By screening the worker（Apis cerana cerana）heads cDNA library, using a fragment of the mrjp3 gene of Apis cerana as probe, 120 positive clones were obtained. The clone containing AccMRJP3 cDNA was selected. Based on the sequencing of the inserts of the positive clone, a sequence of AccMRJP3 cDNA which is 1 887 bp long including a poly(A) tail was obtained. The AccMRJP3 cDNA encompassed an open-reading frame (ORF) with 1 779 bp encoding 593 amino acids. The un-translated regions (UTR) of the 5′end and 3′end are 46 bp and 160 bp long, respectively. Similar to AmMRJP3 and AdMRJP3, the putative AccMRJP3 also has a repetitive region. The comparison of the repetitive region of AccMRJP3, AmMRJP3 and AdMRJP3 shows some differences between them.

Key words: Apis cerana cerana, mrjp3, Gene cloning, Sequence analysis

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