Abstract: The contents of glucosinolates components of 499 rapeseed accessions collected widely in China were analyzed with high performance liquid chromatography (HPLC). The results showed that progoitrin and gluconapin were the main components in high and middle glucosinolate rapeseed, with the percentage of 81.16% and 73.92%. 4-hydroxyglucobrassicin and gluconasturtin were the main components in low glucosinolate rapeseed, with the contents of 7.36 ?mol·g-1 and 4.38 ?mol·g-1, and the percentage reached as high as 51.52%. Coefficient Variability and the effect of individual glucosinolate on total glucosinolate content were analyzed. Progoitrin and gluconapin were the dominant and stable components in double high rapeseed, while their content and percentage were highly reduced in double low rapeseed. 4-hydroxyglucobrassicin concentration was from 7.98 ?mol·g-1 to 6.04 ?mol·g-1, and it was the dominant component in low glucosinolate rapeseed. Gluconasturtin in low glucosinolate rapeseed was much higher than that in double high rapeseed. The possible ways for further reduction of total glucosinolates in rapeseed breeding was discussed.

Key words: Brassica napus, Glucosinolate, Component, Profile