csi-miR399响应柑橘溃疡病菌侵染的表达模式及其抗病性分析

doi:10.3864/j.issn.0578-1752.2023.08.005

Abstract

Abstract:

【Objective】The objective of this study is to identify the expression pattern of csi-miR399 in response to the infection of citrus canker bacteria (Xanthomonas citri subsp. citri, Xcc), screen its target genes, analyze the correlation between csi-miR399 and Xcc resistance in host plants, and to lay a foundation for the creation of citrus canker resistant germplasms.【Method】In order to clarify the expression pattern of csi-miR399 in response to Xcc infection, Xcc-resistant variety Calamondin (Citrus microcarpa) Xcc-sensitive variety Newhall Navel Orange (Citrus sinensis) were used as materials, and changes in the relative expression of csi-miR399 were analyzed by stem-loop qPCR after their leaves were injected with Xcc at 1, 3 and 5 d in vitro. The online software psRNATarget was used to predict the target genes of csi-miR399, which were further confirmed by qPCR in citrus leaves infected with Xcc and transiently over-expressed with csi-miR399. The viral expression vector pCLBV202-MIR399 was constructed by in-fusion cloning through csi-miR399 precursor sequence being inserted into pCLBV202, and transferred into Eureka Lemon (Citrus limon) by Agrobacterium tumefaciens-mediated vacuum infiltration. The lemon over-expressed with csi-miR399 was evaluated for resistance against Xcc through being stab-inoculated with the pathogen and investigated disease index.【Result】After inoculation with Xcc, the expression of csi-miR399 in Calamondin showed a downward trend and then an upward trend, while that in Newhall Navel Orange continued to decrease. At 5 d, the expression of csi-miR399 in Calamondin and Newhall Navel Orange was 4.64 times and 7.61% as its expression in healthy leaves, respectively, preliminary indicating that csi-miR399 was related to citrus canker resistance. Thirteen predicted target genes were screened from citrus genome. Three of them were confirmed because of the opposite expression trends with csi-miR399, which were Cs2g06030 (PHO2), Cs7g03830 (unknown protein), and Cs8g18800 (laccase). Three lemon strains (Y37, Y41 and Y57) with over-expressed csi-miR399 were obtained. Comparing with L35 (empty vector pCLBV202), csi-miR399 was significantly up-regulated in the Y37, Y41 and Y57 strains. The area of canker lesions in Y37, Y41 and Y57 was also significantly reduced, and the disease index was significantly decreased after inoculation with Xcc (P<0.01). It indicated that overexpression of csi-miR399 significantly enhanced the resistance to citrus canker.【Conclusion】csi-miR399 is closely related to the resistance of citrus to canker disease. Overexpression of csi-miR399 significantly improves the resistance, which can be applied to the molecular breeding of citrus against canker disease.

Key words: csi-miR399, citrus canker, target gene, over-expression vector based on citrus virus, biotic stress

WANG ZhaoHao, GUO XingRu, ZHANG LeHuan, HE YongRui, CHEN ShanChun, YAO LiXiao. Expression Pattern of csi-miR399 in Response to Xanthomonas citri subsp. citri Infection and Its Disease Resistance Analysis[J].Scientia Agricultura Sinica, 2023, 56(8): 1484-1493.

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References 41

[1]	AL-SAADI A, REDDY J D, DUAN Y P, BRUNINGS A M, YUAN Q, GABRIEL D W. All five host-range variants of Xanthomonas citri carry one pthA homolog with 17.5 repeats that determines pathogenicity on citrus, but none determine host-range variation. Molecular Plant-Microbe Interactions, 2007, 20(8): 934-943. doi: 10.1094/mpmi-20-8-0934. doi: 10.1094/mpmi-20-8-0934
[2]	PITINO M, ARMSTRONG C M, DUAN Y P. Rapid screening for citrus canker resistance employing pathogen-associated molecular pattern-triggered immunity responses. Horticulture Research, 2015, 2: 15042. doi: 10.1038/hortres.2015.42. doi: 10.1038/hortres.2015.42 pmid: 26504581
[3]	王晓宇, 彭埃天, 宋晓兵, 黄峰, 崔一平. 柑橘溃疡病综合防控技术研究进展. 中国农学通报, 2021, 37(31): 106-111.
	WANG X Y, PENG A T, SONG X B, HUANG F, CUI Y P. Comprehensive control technology of citrus bacterial canker disease: A review. Chinese Agricultural Science Bulletin, 2021, 37(31): 106-111. (in Chinese)
[4]	ISLAM M N, ALI M S, CHOI S J, HYUN J W, BAEK K H. Biocontrol of citrus canker disease caused by Xanthomonas citri subsp. citri using an endophytic Bacillus thuringiensis. Plant Pathology Journal, 2019, 35(5): 486-497. doi: 10.5423/PPJ.OA.03.2019.0060. doi: 10.5423/PPJ.OA.03.2019.0060
[5]	VILLAMIZAR S, FERRO J A, CAICEDO J C, ALVES L M C. Bactericidal effect of entomopathogenic bacterium Pseudomonas entomophila against Xanthomonas citri reduces citrus canker disease severity. Frontiers in Microbiology, 2020, 11: 1431. doi: 10.3389/fmicb.2020.01431. doi: 10.3389/fmicb.2020.01431
[6]	CONTI G, XOCONOSTLE-CAZARES B, MARCELINO-PEREZ G, HOPP H E, REYES C A. Citrus genetic transformation: An overview of the current strategies and insights on the new emerging technologies. Frontiers in Plant Science, 2021, 12: 768197. doi: 10.3389/fpls.2021.768197. doi: 10.3389/fpls.2021.768197
[7]	姚利晓, 范海芳, 张庆雯, 何永睿, 许兰珍, 雷天刚, 彭爱红, 李强, 邹修平, 陈善春. 柑橘溃疡病抗性相关转录因子CitMYB20的功能. 中国农业科学, 2020, 53(10): 1997-2008. doi: 10.3864/j.issn.0578-1752.2020.10.007. doi: 10.3864/j.issn.0578-1752.2020.10.007
	YAO L X, FAN H F, ZHANG Q W, HE Y R, XU L Z, LEI T G, PENG A H, LI Q, ZOU X P, CHEN S C. Function of citrus bacterial canker resistance-related transcription factor CitMYB20. Scientia Agricultura Sinica, 2020, 53(10): 1997-2008. doi: 10.3864/j.issn.0578-1752.2020.10.007. (in Chinese) doi: 10.3864/j.issn.0578-1752.2020.10.007
[8]	PENG A H, CHEN S C, LEI T G, XU L Z, HE Y R, WU L, YAO L X, ZOU X P. Engineering canker-resistant plants through CRISPR/Cas9- targeted editing of the susceptibility gene CsLOB1 promoter in citrus. Plant Biotechnology Journal, 2017, 15(12): 1509-1519. doi: 10.1111/pbi.12733. doi: 10.1111/pbi.12733
[9]	CHEN X M. Small RNAs and their roles in plant development. Annual Review of Cell and Developmental Biology, 2009, 25: 21-44. doi: 10.1146/annurev.cellbio.042308.113417. doi: 10.1146/annurev.cellbio.042308.113417 pmid: 19575669
[10]	VAUCHERET H. Post-transcriptional small RNA pathways in plants: Mechanisms and regulations. Genes & Development, 2006, 20(7): 759-771. doi: 10.1101/gad.1410506. doi: 10.1101/gad.1410506
[11]	YANG X, ZHANG L, YANG Y, SCHMID M, WANG Y. miRNA mediated regulation and interaction between plants and pathogens. International Journal of Molecular Sciences, 2021, 22(6): 2913. doi: 10.3390/ijms22062913. doi: 10.3390/ijms22062913
[12]	NAVARRO L, DUNOYER P, JAY F, ARNOLD B, DHARMASIRI N, ESTELLE M, VOINNET O, JONES J D. A plant miRNA contributes to antibacterial resistance by repressing auxin signaling. Science, 2006, 312(5772): 436-439. doi: 10.1126/science.1126088. doi: 10.1126/science.1126088 pmid: 16627744
[13]	CARUANA J C, DHAR N, RAINA R. Overexpression of Arabidopsis microRNA167 induces salicylic acid-dependent defense against Pseudomonas syringae through the regulation of its targets ARF6 and ARF8. Plant Direct, 2020, 4(9): e00270. doi: 10.1002/pld3.270. doi: 10.1002/pld3.270
[14]	LI Y, LU Y G, SHI Y, WU L, XU Y J, HUANG F, GUO X Y, ZHANG Y, FAN J, ZHAO J Q, et al. Multiple rice microRNAs are involved in immunity against the blast fungus Magnaporthe oryzae. Plant Physiology, 2014, 164(2): 1077-1092. doi: 10.1104/pp.113.230052. doi: 10.1104/pp.113.230052
[15]	LI Y, CAO X L, ZHU Y, YANG X M, ZHANG K N, XIAO Z Y, WANG H, ZHAO J H, ZHANG L L, LI G B, et al. Osa-miR398b boosts H₂O₂ production and rice blast disease-resistance via multiple superoxide dismutases. New Phytologist, 2019, 222(3): 1507-1522. doi: 10.1111/nph.15678. doi: 10.1111/nph.15678
[16]	LUAN Y S, CUI J, LI J, JIANG N, LIU P, MENG J. Effective enhancement of resistance to Phytophthora infestans by overexpression of miR172a and b in Solanum lycopersicum. Planta, 2018, 247(1): 127-138. doi: 10.1007/s00425-017-2773-x. doi: 10.1007/s00425-017-2773-x
[17]	FAN D, RAN L Y, HU J, YE X, XU D, LI J Q, SU H L, WANG X Q, REN S, LUO K M. miR319a/TCP module and DELLA protein regulate trichome initiation synergistically and improve insect defenses in Populus tomentosa. New Phytologist, 2020, 227(3): 867-883. doi: 10.1111/nph.16585. doi: 10.1111/nph.16585
[18]	ZHENG Z, WANG N, JALAJAKUMARI M, BLACKMAN L, SHEN E, VERMA S, WANG M B, MILLAR A A. miR159 represses a constitutive pathogen defense response in tobacco. Plant Physiology, 2020, 182(4): 2182-2198. doi: 10.1104/pp.19.00786. doi: 10.1104/pp.19.00786 pmid: 32041907
[19]	MISHRA R, MOHANTY J N, CHAND S K, JOSHI R K. Can-miRn37a mediated suppression of ethylene response factors enhances the resistance of chilli against anthracnose pathogen Colletotrichum truncatum L. Plant Science, 2018, 267: 135-147. doi: 10.1016/j.plantsci.2017.12.001. doi: 10.1016/j.plantsci.2017.12.001
[20]	XU Y, WANG R, MA P, CAO J, CAO Y, ZHOU Z, LI T, WU J, ZHANG H. A novel maize microRNA negatively regulates resistance to Fusarium verticillioides. Molecular Plant Pathology, 2022, 23(10): 1446-1460. doi: 10.1111/mpp.13240. doi: 10.1111/mpp.13240
[21]	BARI R, PANT B D, STITT M, SCHEIBLE W R. PHO2, microRNA399, and PHR1 define a phosphate-signaling pathway in plants. Plant Physiology, 2006, 141(3): 988-999. doi: 10.1104/pp.106.079707. doi: 10.1104/pp.106.079707 pmid: 16679424
[22]	PANT B D, BUHTZ A, KEHR J, SCHEIBLE W R. MicroRNA 399 is a long-distance signal for the regulation of plant phosphate homeostasis. The Plant Journal, 2008, 53(5): 731-738. doi: 10.1111/j.1365-313X.2007.03363.x. doi: 10.1111/j.1365-313X.2007.03363.x
[23]	WANG R, FANG Y N, WU X M, QING M, LI C C, XIE K D, DENG X X, GUO W W. The miR399-CsUBC24 module regulates reproductive development and male fertility in citrus. Plant Physiology, 2020, 183(4): 1681-1695. doi: 10.1104/pp.20.00129. doi: 10.1104/pp.20.00129
[24]	WANG Y, ZHANG J, CUI W, GUAN C, MAO W, ZHANG Z. Improvement in fruit quality by overexpressing miR399a in woodland strawberry. Journal of Agricultural and Food Chemistry, 2017, 65(34): 7361-7370. doi: 10.1021/acs.jafc.7b01687. doi: 10.1021/acs.jafc.7b01687 pmid: 28783952
[25]	HU Z, WANG F S, YU H, ZHANG M M, JIANG D, HUANG T J, XIANG J S, ZHU S P, ZHAO X C. Effects of scion-rootstock interaction on citrus fruit quality related to differentially expressed small RNAs. Scientia Horticulturae, 2022, 298: 110974. doi: 10.1016/j.scienta.2022.110974. doi: 10.1016/j.scienta.2022.110974
[26]	VAL-TORREGROSA B, BUNDO M, MARTIN-CARDOSO H, BACH-PAGES M, CHIOU T J, FLORS V, SEGUNDO B S. Phosphate-induced resistance to pathogen infection in Arabidopsis. The Plant Journal, 2022, 110(2): 452-469. doi: 10.1111/tpj.15680. doi: 10.1111/tpj.15680
[27]	ZHANG Q, ZHANG Y, WANG S, HAO L, WANG S, XU C, JIANG F, LI T. Characterization of genome-wide microRNAs and their roles in development and biotic stress in pear. Planta, 2019, 249(3): 693-707. doi: 10.1007/s00425-018-3027-2. doi: 10.1007/s00425-018-3027-2 pmid: 30368557
[28]	ZHAO H W, SUN R B, ALBRECHT U, PADMANABHAN C, WANG A R, COFFEY M D, GIRKE T, WANG Z H, CLOSE T J, ROOSE M, et al. Small RNA profiling reveals phosphorus deficiency as a contributing factor in symptom expression for citrus Huanglongbing disease. Molecular Plant, 2013, 6(2): 301-310. doi: 10.1093/mp/sst002. doi: 10.1093/mp/sst002 pmid: 23292880
[29]	DAI X, ZHUANG Z, ZHAO P X. psRNATarget: A plant small RNA target analysis server (2017 release). Nucleic Acids Research, 2018, 46(W1): W49-W54. doi: 10.1093/nar/gky316. doi: 10.1093/nar/gky316
[30]	LI F, DAI S, DENG Z, LI D, LONG G, LI N, LI Y, GENTILE A. Evaluation of parameters affecting Agrobacterium-mediated transient expression in citrus. Journal of Integrative Agriculture, 2017, 16(3): 572-579. doi: 10.1016/S2095-3119(16)61460-0. doi: 10.1016/S2095-3119(16)61460-0
[31]	ACANDA Y, WELKER S, ORBOVIĆ V, LEVY A. A simple and efficient agroinfiltration method for transient gene expression in citrus. Plant Cell Reports, 2021, 40(7): 1171-1179. doi: 10.1007/s00299-021-02700-w. doi: 10.1007/s00299-021-02700-w pmid: 33948685
[32]	张琦, 段玉, 苏越, 蒋琪琪, 王春庆, 宾羽, 宋震. 基于柑橘叶斑驳病毒的表达载体构建及应用. 中国农业科学, 2022, 55(22): 4398-4407. doi: 10.3864/j.issn.0578-1752.2022.22.006. doi: 10.3864/j.issn.0578-1752.2022.22.006
	ZHANG Q, DUAN Y, SU Y, JIANG Q Q, WANG C Q, BIN Y, SONG Z. Construction and application of expression vector based on citrus leaf blotch virus. Scientia Agricultura Sinica, 2022, 55(22): 4398-4407. doi: 10.3864/j.issn.0578-1752.2022.22.006. (in Chinese) doi: 10.3864/j.issn.0578-1752.2022.22.006
[33]	SIMMONS C W, VANDERGHEYNST J S, UPADHYAYA S K. A model of Agrobacterium tumefaciens vacuum infiltration into harvested leaf tissue and subsequent in planta transgene transient expression. Biotechnology and Bioengineering, 2009, 102(3): 965-970. doi: 10.1002/bit.22118. doi: 10.1002/bit.22118
[34]	CAMPOS-SORIANO L, BUNDO M, BACH-PAGES M, CHIANG S F, CHIOU T J, SEGUNDO B S. Phosphate excess increases susceptibility to pathogen infection in rice. Molecular Plant Pathology, 2020, 21(4): 555-570. doi: 10.1111/mpp.12916. doi: 10.1111/mpp.12916
[35]	PEGLER J L, NGUYEN D Q, OULTRAM J M J, GROF C P L, EAMENS A L. Molecular manipulation of the miR396 and miR399 expression modules alters the response of Arabidopsis thaliana to phosphate stress. Plants, 2021, 10(12): 2570. doi: 10.3390/plants10122570. doi: 10.3390/plants10122570
[36]	LIN S I, CHIANG S F, LIN W Y, CHEN J W, TSENG C Y, WU P C, CHIOU T J. Regulatory network of microRNA399 and PHO2 by systemic signaling. Plant Physiology, 2008, 147(2): 732-746. doi: 10.1104/pp.108.116269. doi: 10.1104/pp.108.116269
[37]	PENG K K, TIAN Y, SUN X Z, SONG C H, REN Z P, BAO Y Z, XING J P, LI Y S, XU Q H, YU J, ZHANG D, CANG J. tae-miR399-UBC24 module enhances freezing tolerance in winter wheat via a CBF signaling pathway. Journal of Agricultural and Food Chemistry, 2021, 69(45): 13398-13415. doi: 10.1021/acs.jafc.1c04316. doi: 10.1021/acs.jafc.1c04316
[38]	ZHOU H, LI C, QIU X, LU S. Systematic analysis of alkaline/neutral invertase genes reveals the involvement of Smi-miR399 in regulation of SmNINV3 and SmNINV4 in Salvia miltiorrhiza. Plants, 2019, 8(11): 490. doi: 10.3390/plants8110490. doi: 10.3390/plants8110490
[39]	BAEK D, CHUN H J, KANG S, SHIN G, PARK S J, HONG H, KIM C, KIM D H, LEE S Y, KIM M C, YUN D J. A role for Arabidopsis miR399f in salt, drought, and ABA signaling. Molecules and Cells, 2016, 39(2): 111-118. doi: 10.14348/molcells.2016.2188. doi: 10.14348/molcells.2016.2188
[40]	BAI Y, ALI S, LIU S, ZHOU J, TANG Y. Characterization of plant laccase genes and their functions. Gene, 2023, 852: 147060. doi: 10.1016/j.gene.2022.147060. doi: 10.1016/j.gene.2022.147060
[41]	VELAZQUEZ K, AGUERO J, VIVES M C, ALEZA P, PINA J A, MORENO P, NAVARRO L, GUERRI J. Precocious flowering of juvenile citrus induced by a viral vector based on citrus leaf blotch virus: A new tool for genetics and breeding. Plant Biotechnology Journal, 2016, 14(10): 1976-1985. doi: 10.1111/pbi.12555. doi: 10.1111/pbi.12555 pmid: 26920394

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引物名称 Primer name	核苷酸序列 Nucleotide sequence (5′-3′)
csi-Actin-F	CATCCCTCAGCACCTTCC
csi-Actin-R	CCAACCTTAGCACTTCTCC
miR399-RT	CTCAACTGGTGTCGTGGAGTCCGGCAATTCAGTTGAGCAGGGCAA
miR399-F	ACACTCCAGCTGGGTGCCAAAGGA
Universal-miR	AACTGGTGTCGTGGAG
Cs2g02870F	AATGCCGCTATTTCTGTCCCA
Cs2g02870R	GCTCCTCAGCCAGGTTTGTT
Cs2g06030F	GAGCTTGTGGAGGAGCACTT
Cs2g06030R	CCCACTGAACATCCGTTGGA
Cs2g14510F	AGGAATTCAGGCAGCCCATC
Cs2g14510R	CGACTTGGCTTCTTCCCCTT
Cs3g09820F	CGGCAACTTGAGCAAAGCAT
Cs3g09820R	GAACGCTTGGAAGGCTCAAC
Cs4g04510F	TCAAATCCCCAAGCCTTTGC
Cs4g04510R	AACAGAACAAGTCAGCGAGT
Cs7g03830F	ATAGCCGGTGTGCTCATTGT
Cs7g03830R	TCCCAGTACACTCGCAAGGA
Cs7g08000F	TATTCCGGTGCGTGTGATCG
Cs7g08000R	ATTTGACACAACCGCAAGGC
Cs7g22930F	AGAGGTGGGCCTCAATTTGG
Cs7g22930R	AGCTTGCCTTAGGTCACCAAT
Cs8g05410F	ACCACCTGATGCTCCCCTTA
Cs8g05410R	TGATCCGACGGCAGATAGTG
Cs8g18800F	ACACAGCCGATTTCCCACAA
Cs8g18800R	ATGAAGAGGGTGGCTTTCGG
1.1g025013mF	CGTCAAACTCCAACGTGCTT
1.1g025013mR	ATCATACCCCTGGCGAAGTC
1.1t01536F	GGTCTTGCAACCAGGGATCA
1.1t01536R	ACTGCCTGTGATGATCTGCC
1.1t02010F	CCACCTGGATCTAAGGGCAA
1.1t02010R	CATTTGCTGGAAAAGCGCCA

引物名称 Primer name	核苷酸序列 Nucleotide sequence (5′-3′)
pLGN-MIR399-F	GGGTACCCGGGGATCAAAGCAGTTTTAGGGCACCTCTT
pLGN-MIR399-R	TTAAAGCAGGGAATTGAAGCAGTCACAGGGCAACTCT
pCLBV202-MIR399-F	AGATTGAGAAAACCCAAAGCAGTTTTAGGGCACC
pCLBV202-MIR399-R	CAGAATTCGGGACCCGAAGCAGTCACAGGGCAAC
pClbv-s	CTGATGGGAGCGTTAGACTGA
pClbv-r	TGTAAAGTCCTGGCCCACC

Expression Pattern of csi-miR399 in Response to Xanthomonas citri subsp. citri Infection and Its Disease Resistance Analysis

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