Scientia Agricultura Sinica ›› 2023, Vol. 56 ›› Issue (24): 4801-4813.doi: 10.3864/j.issn.0578-1752.2023.24.001


Research Progress of PPR Protein in Plant Abiotic Stress Response

LI Cheng(), LU Kai, WANG CaiLin, ZHANG YaDong()   

  1. Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/East China Branch of National Center of Technology Innovation for Saline-Alkali Tolerant Rice/Jiangsu High Quality Rice R&D Center/Nanjing Branch of China National Center for Rice Improvement/Key laboratory of Jiangsu Province for Agrobiology, Nanjing 210014
  • Received:2023-05-25 Accepted:2023-07-24 Online:2023-12-16 Published:2023-12-21
  • Contact: ZHANG YaDong


Abiotic stress is one of the main factors causing global grain yield reduction. It is of great significance to study the function and response mechanisms of plant stress-related proteins to improve crop stress resistance. Pentatricopeptide repeat (PPR) proteins, belong to the largest family of nuclear coding proteins in higher plants and are named because they contain highly specific PPR motifs. Depending on motif type and arrangement, PPR proteins can be classified as P and PLS, and PLS proteins can be further classified as PLS, E, E+, DYW, and other subclasses based on their carboxyl-terminal domains. PPR proteins are widely distributed in terrestrial plants, mainly in chloroplasts and mitochondria, and a few in the nucleus. As sequence-specific RNA binding proteins, PPR proteins are involved in multiple aspects of plant RNA processing, including RNA editing, splicing, stabilization, and translation. PPR protein plays a variety of important roles in the whole life process of plants, but the mechanism of its action in plant stress resistance is not well understood. Based on the localization and function of PPR proteins related to abiotic stress reported, the mechanism of PPR proteins involved in regulation of abiotic stress, including post-transcriptional regulation and retrograde signaling, was reviewed and discussed in this paper. Post-transcriptional regulation is related to the role of PPR proteins in the modification of RNA after transcription. It is generally believed that PPR affects stress resistance in plants by regulating the expression of stress-related genes via binding RNA and by regulating the metabolism of organelle RNA. In terms of retrograde signaling, damage to PPR proteins can lead to impaired mitochondrial or chloroplast function, and then produce various retrograde signals (such as ROS), thereby regulating the expression of related genes and resisting adversity. However, since plastid signaling is affected by many environmental factors, some of which are still unclear, the mechanism of the PPR protein in retrograde signaling remains to be clarified. In addition, PPR proteins are pleiotropic and some have important effects on plant growth and reproduction while acting on stress resistance. Finally, this paper further analyzed the current research status of PPR protein as an RNA editing tool, discussed the remaining problems and research prospects of PPR protein in the direction of abiotic stress, and pointed out the key points and difficulties that need to be paid attention to in future research, to provide references for further research on PPR protein and crop abiotic stress resistance breeding.

Key words: PPR protein, plant, abiotic stress

Fig. 1

Main classification of PPR protein family"

Table 1

Some of PPR proteins involved regulating abiotic stress responses in plant"

RARE1 拟南芥 Arabidopsis 叶绿体 Chloroplast 耐热 Heat resistance [50]
SVR7 拟南芥 Arabidopsis 叶绿体 Chloroplast 光氧化 Photooxidation [51]
GUN1 拟南芥
Sucrose, ABA, photooxidation, heat resistance
Salt, ABA, oxidative stress
ABA [59]
PGN 拟南芥 Arabidopsis 线粒体 Mitochondrion ABA、葡萄糖、盐 ABA, glucose, salt [61]
ABA, drought
SLO2 拟南芥
ABA, salt, drought, osmotic stress
SOAR1 拟南芥
Nucleus and cytoplasm
ABA, drought, salt, cold
- 耐热
Heat resistance
Oryza sativa
Low temperature
WSL 水稻 Oryza sativa 叶绿体 Chloroplast ABA、盐、糖 ABA, salt, sugar [74]
PPS1 水稻 Oryza sativa 线粒体 Mitochondrion 盐、ABA Salt, ABA [75]
OsNBL3 水稻 Oryza sativa 线粒体 Mitochondrion 盐 Salt [76]
Oryza sativa
OsSOAR1 水稻 Oryza sativa - 盐 Salt [78]
GmPPR4 大豆 Glycine max - 干旱 Drought [79]
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