189份桃种质肉质性状形成相关位点基因型鉴定及组合分析

doi:10.3864/j.issn.0578-1752.2023.12.011

Abstract

Abstract:

【Objective】 The molecular markers and bioinformatics were used to identify the genotypes and combinations of the F-M locus, transposon insertion in YUC11 promoter region, and 9 bp insert in NAC72 coding region by 189 peach germplasms, in order to provide the theoretical foundation for the mechanism of peach flesh formation and the selection of breeding parents.【Method】PCR amplification, KASP, and HRM were used to identify genotypes. Results of loci associated with the formation of flesh trait were further validated using bioinformatics by resequencing data in 189 peach germplasms.【Result】Two genes encoding endopolygalacturonase in F-M locus, designated PGM and PGF, are associated with peach texture. Through primer amplification, 159 (84%) PGM and 99 (52%) PGF were detected in 189 peach germplasms. Four haplotypes (H0, H1, H2, and H3) were found in F-M locus, while H0 and H1 were the major genotypes. The haplotype combination of non-melting flesh, melting flesh, free-stone melting flesh were H3H3 and H2H3, H0H0 and H0H1, H1H1 and H0H1, respectively. In addition, it was found that 18 germplasms contain homozygous transposon insertion. HRM results demonstrated that 45 germplasms were homozygous insertion (early ripening), 71 germplasms were heterozygous insertion (middle ripening), and 73 germplasms were no insertion (late ripening). But six germplasm genotypes were inconsistent with the phenotypes. The most frequent genotypic combinations at the three loci of non-melting flesh, melting flesh, and free-stone melting flesh were mmffHdHdI, MMffHdHdI, MMFFHdHdL, respectively.【Conclusion】The existence of four haplotypes at the F-M locus further was confirmed, 18 germplasms containing stony hard genotype were identified, a molecular marker for the identification of maturity date was developed, and genotype combinations of different germplasms at flesh-related loci were identified.

Key words: peach, flesh texture, genotype identification, genotype combinations

WANG ZhaoHui, LI Yong, CAO Ke, ZHU GengRui, FANG WeiChao, CHEN ChangWen, WANG XinWei, WU JinLong, WANG LiRong. Genotype Identification and Combination Analysis of Loci Related to the Peach Flesh Texture Trait via 189 Peach Accessions[J].Scientia Agricultura Sinica, 2023, 56(12): 2367-2379.

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References 48

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名称 Code	引物序列 Primer sequence	退火温度 Annealing temperature (℃)	片段长度 Size (bp)
PGM-F	ACTAACCCAACCAATTACTCAAACC	56	3066
PGM-R	TGTCCTTAGTACTGGAGGGCAAAC
PGF-F	AGGAGAAATTGGAGACGCCG	58	3353
PGF-R	CGTCTTCCGGACATAATCTTACA
YUC-F	TAAAGCCGCCCAAAAATAAA	58	600
YUC-R1	TGGGAAGGAAGAAAATAGTCACA
YUC-R2	ATTTTCAACTTTCCCGAGCA	58	448/3015
PGH0-F	TACGACTACAAGCCAAGTCGG	56	591
PGH0-R	ACTCCAGTTGCGCTGAAAGA
PGH2-F	CATATTGAAGAACCAGCCA	56	1364
PGH2-R	TGGTATATTGCATCGACGTG
PGH3-F	AAAGATAGGAAGGGGGAAGG	56	524
PGH3-R	GTTTGCACTGGGGGTTTTAGA

表型 Phenotype				基因型出现个数 Number of genotype occurrences
溶质粘核		溶质离核	不溶质粘核	1	2	3	4
MMffHdHdI	MMFFHdHdL	MMFFHdHdL	mmffHdHdL	12	2	22	11
MMFFHdHdE	MmffHdhdI	MMFfHdhdI	mmffHdHdE	9	1	8	5
MMFfHdHdI	MmffhdhdE	MMFfHdHI	mmffHdHdI	9	1	7	5
MMffHdHdL	MMFFHdhdE	MMFfHdHdL	MmffHdHdE	8	1	5	3
MMFfHdHdE	MMFFHdhdL	MmFfHdHdL	MMffHdHdI	4	1	5	3
MmFfHdhdE	MMFfHdhdI	MMFFhdhdI	MMffHdHdL	4	1	4	2
MmffHdHdL	MmFfHdHdI	MMffHdHdL	mmffhdhdE	4	1	3	2
MmffHdHdL	MmFfHdHdL	MmFfHdhdE	MmffHdHdI	4	1	2	1
MMffhdhdI	MMffHdHdE	MmFfHdhdI	MmffHdHdL	3	1	2	1
MmffHdHdI	MMffHdhdI	MMFFHdHdI	MmffHdhdI	3	1	2	1
MmFfHdHdE	MMffHdhdI	MMFfhdhdE	MMffHdhdI	3	1	1	1
MMFFHdHdI	MMffhdhdL	MmFfHdHdE	MmFfHdHdI	2	1	1	1
MMffHdhdL		MMFFhdhdL	MMFfHdHdI	2		1	1
MMffhdhdE		MMFFhdhdE		2		1
MmffHdHdE		MmFfhdhdE		2		1
mmffHdHdE		MMFfHdhdE		2		1

Genotype Identification and Combination Analysis of Loci Related to the Peach Flesh Texture Trait via 189 Peach Accessions

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