122份栽培桃品种（系）黄白肉性状的分子标记辅助鉴定

doi:10.3864/j.issn.0578-1752.2020.14.016

Abstract

Abstract:

【Objective】It shows that peach flesh color (yellow/white) is controlled by the gene CCD 4 (carotenoid cleavage dioxygenase 4). Based on three types of CCD4 allele variations, molecular markers of indel, SSR-CE, and Sanger sequence for SNP were used to analyze the genotypes of 122 peach cultivars (lines), with the aim to determine the correlation between flesh color and genetic variation. This result could provide information for parental matching and the selection of corresponding molecular markers for phenotyping in their offspring. 【Method】Three types of variations were detected via PCR. LTR transposable element insertion was detected by 1% agarose gel electrophoresis, and SSR repeat numbers were detected using CE-SSR in ABI3730XL. Nucleotide substitution was detected using the Sanger sequence and analyzed with the ContigExpress software. In total, the genotypes of 122 cultivars (lines) were analyzed, and the correlation between phenotype and genetic variation was determined.【Result】After genotyping of the 122 cultivars, it was found that 31 accessions were LTR transposable element insertions with 729-bp amplified fragments, accounting for 25.4% of the total accessions, of which eight accessions (25.8%) were homozygous. Sixty-eight cultivars (lines) were SSR repeat number variations, accounting for 55.7% of the total accessions and including 25 (36.8%) homozygous types with 2-bp insertions. Of the 122 cultivars (lines), only one cultivar (Fertilia Morettini) was caused by nucleotide substitution and SSR repeat number variation, accounting for 0.82%, which was not widely used in the breeding program. LTR transposable element insertion and SSR repeat number variation were the key types affecting flesh color. Of the 122 cultivars (lines), seven yellow-flesh cultivars (lines) were caused by SSR repeat number variation and LTR transposable element insertion, accounting for 5.7%. One homozygous or two heterozygous sequence variations were both responsible for yellow flesh. The results showed that genotypes were identical with phenotypes of the 122 accessions, with 100% accuracy. 【Conclusion】The genotypes of 122 peach cultivars (lines) (white and yellow flesh color) were identified using molecular markers, which could be applied for parental selection, offspring identification in breeding programs, and flesh color selection (white or yellow) using molecular marker-assisted selection.

Key words: peach, CCD4, MAS, flesh color

LU ZhenHua,SHEN ZhiJun,NIU Liang,PAN Lei,CUI GuoChao,ZENG WenFang,WANG ZhiQiang. Molecular Marker-Assisted Identification of Yellow/White Flesh Trait for 122 Peach Cultivars (Lines)[J].Scientia Agricultura Sinica, 2020, 53(14): 2929-2940.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2020.14.016

https://www.chinaagrisci.com/EN/Y2020/V53/I14/2929

Figures/Tables 7

Table 1

Allelic variants of CCD4 as related to phenotype in yellow/white flesh peach"

编号 Code	品种（系） Cultivar (line)	来源 Origin	果肉类型 Flesh color	微卫星序列 SSR	SNP位点 SNP	CCD4/LTR CCD4/LTR
1	雨花2号 Yuhua 2	NFGRN	白 White	177/177	AA	1/1
2	霞脆 Xiacui	NFGRN	白 White	177/177	AA	1/1
3	雨花1号 Yuhua 1	NFGRN	白 White	177/177	AA	1/0
4	瑞光18号 Ruiguang 18	NFGRN	黄Yellow	179/179	AA	1/0
5	瑞光19号 Ruiguang 19	NFGRN	白 White	177/179	AA	1/0
6	早美Zaomei	NFGRN	白 White	177/177	AA	1/0
7	红粉佳人 Pinklady	NFGRN	白 White	177/177	AA	1/0
8	瑞光美玉 Ruiguangmeiyu	NFGRN	白 White	177/179	AA	1/0
9	早花露 Zaohualu	NFGRN	白 White	177/177	AA	1/0
10	大久保 Okubo	NFGRN	白 White	177/177	AA	1/0
11	金陵黄露 Jinlinghuanglu	NFGRN	黄 Yellow	179/179	AA	1/0
12	沪油002号 Huyou 002	NFGRN	白 White	177/179	AA	1/0
13	锦绣 Jinxiu	NFGRN	黄 Yellow	177/177	AA	0/1
14	金童7号 Babygold 7	NFGRN	黄 Yellow	177/179	AA	1/1
15	沪油003号 Huyou 003	NFGRN	黄 Yellow	177/179	AA	1/1
16	玉霞蟠桃 Yuxiapantao	NFGRN	白 White	177/179	AA	1/0
17	五月火 Mayfire	NFGRN	黄 Yellow	179/179	AA	1/0
18	早凤玉 Zaofengyu	NFGRN	白 White	177/179	AA	1/0
19	沪021号 Hu 021	NFGRN	白 White	177/177	AA	1/1
20	日川白凤 Richuanbaifeng	NFGRN	白 White	177/177	AA	1/1
21	瑞光7号 Ruiguang 7	NFGRN	白 White	177/179	AA	1/0
22	瑞光22号 Ruiguang 22	NFGRN	黄 Yellow	179/179	AA	1/0
23	阿姆肯 Armking	NFGRN	黄 Yellow	179/179	AA	1/0
24	霞晖2号 Xiahui 2	NFGRN	白 White	177/177	AA	1/0
25	瑞蟠3号 Ruipan 3	NFGRN	白 White	177/177	AA	1/0
26	早金露 Zaojinlu	NFGRN	黄 Yellow	177/179	AA	1/1
27	瑞蟠5号 Ruipan 5	NFGRN	白 White	177/177	AA	1/1
28	晚硕蜜 Wanshuomi	NFGRN	白 White	177/177	AA	1/0
29	早露蟠桃 Zaolupantao	NFGRN	白 White	177/177	AA	1/0
30	霞晖8号 Xiahui 8	NFGRN	白 White	177/179	AA	1/0
31	晚白花 Wanbaihua	NFGRN	白 White	177/177	AA	1/1
32	连黄 Lianhuang	NFGRN	黄 Yellow	177/179	AA	0/1
33	春花 Chunhua	NFGRN	白 White	177/177	AA	1/1
34	黄露蟠桃 Huanglupantao	NFGRN	白 White	177/177	AA	1/1
35	金童5号 Babygold 5	NFGRN	黄 Yellow	177/179	AA	1/1
36	砂子早生 Sunago Wase	NFGRN	白 White	177/177	AA	1/1
37	早上海水蜜 Zaoshanghaishuimi	NFGRN	白 White	177/177	AA	1/0
38	金童9号 Babygold 9	NFGRN	黄 Yellow	177/177	AA	0/1
39	紫金红2号 Zijinghong 2	NFGRN	黄 Yellow	179/179	AA	1/0
40	银花露 Yinhualu	NFGRN	白 White	177/177	AA	1/1
编号 Code	品种（系） Cultivar （line）	来源 Origin	果肉类型 Flesh color	微卫星序列 SSR	SNP位点 SNP	CCD4/LTR CCD4/LTR
41	弗雷德里克 Frederica	NFGRN	黄 Yellow	179/179	AA	1/0
42	新白凤 Early Hakuho	NFGRN	白 White	177/177	AA	1/0
43	奉化玉露早 Fenghuayuluzao	NFGRN	白 White	177/177	AA	1/0
44	霞晖4号 Xiahui 4	NFGRN	白 White	177/179	AA	1/0
45	早硕蜜 Zaoshuomi	NFGRN	白 White	177/177	AA	1/0
46	早魁蜜 Zaokuimi	NFGRN	白 White	177/177	AA	1/0
47	霞光 Xiaguang	NFGRN	黄 Yellow	177/179	AA	1/1
48	瑞蟠4号 Ruipan 4	NFGRN	白 White	177/177	AA	1/0
49	京玉 Jingyu	NFGRN	白 White	177/177	AA	1/1
50	金霞蟠桃 Jinxiapantao	NFGRN	黄 Yellow	179/179	AA	1/1
51	京红 Jinghong	NFGRN	白 White	177/177	AA	1/0
52	银河 Galaxy	NFGRN	白 White	177/179	AA	1/0
53	瑞光28号 Ruiguang 28	NFGRN	黄 Yellow	179/179	AA	1/0
54	瑞蟠2号 Ruipan 2	NFGRN	白 White	177/177	AA	1/0
55	金晖 Jinhui	NFGRN	黄 Yellow	177/177	AA	0/1
56	雨花露 Yuhualu	NFGRN	白 White	177/177	AA	1/0
57	白花水蜜 Baihuashuilu	NFGRN	白 White	177/177	AA	1/1
58	丰黄 Fenghuang	NFGRN	黄 Yellow	177/177	AA	0/1
59	晖雨露 Huiyulu	NFGRN	白 White	177/177	AA	1/0
60	奉化玉露 Fenghuayulu	NFGRN	白 White	177/177	AA	1/0
61	花玉露 Huayulu	NFGRN	白 White	177/177	AA	1/0
62	瑞光23 号 Ruiguang 23	NFGRN	白 White	177/179	AA	1/0
63	瑞蟠1号 Ruipan 1	NFGRN	白 White	177/177	AA	1/0
64	霞晖3号 Xiahui 3	NFGRN	白 White	177/177	AA	1/0
65	金童8号 Babygold 8	NFGRN	白 White	177/177	AA	0/1
66	霞晖1号 Xiahui 1	NFGRN	白 White	177/177	AA	1/0
67	雨花3号 Yuhua 3	NFGRN	白 White	177/177	AA	1/1
68	金陵锦桃 Jinlingjintao	NFGRN	白 White	177/177	AA	1/0
69	源东白桃 Yuandongbaitao	NFGRN	白 White	177/177	AA	1/1
70	新白花 Xinbaihua	NFGRN	白 White	177/177	AA	1/1
71	金童6号 Babygold 6	NFGRN	黄 Yellow	177/179	AA	1/1
72	紫金红3号 Zijinhong 3	NFGRN	黄 Yellow	179/179	AA	1/0
73	金山早红 Jinshanzaohong	NFGRN	黄 Yellow	179/179	AA	1/0
74	早红港 Early Redhaven	NFGRN	黄 Yellow	177/177	AA	0/1
75	霞晖5号 Xiahui 5	NFGRN	白 White	177/177	AA	0/0
76	南山甜桃 Nanshantiantao	NFGRN	白 White	177/177	AA	1/0
77	锦香 Jinxiang	NFGRN	黄 Yellow	177/177	AA	0/1
78	弗尔蒂尼莫蒂尼 Fertilia Morettini	NFGRN	黄 Yellow	177/179	AT	1/0
79	京春 Jingchun	NFGRN	白 White	177/177	AA	1/0
80	中蟠1号 Zhongpan 1	ZFRI	白 White	177/179	AA	1/0
81	99-54-36	ZFRI	白 White	177/179	AA	1/0
编号 Code	品种（系） Cultivar （line）	来源 Origin	果肉类型 Flesh color	微卫星序列 SSR	SNP位点 SNP	CCD4/LTR CCD4/LTR
82	36-3	ZFRI	白 White	177/179	AA	1/0
83	黄金蜜1号 Huangjinmi 1	ZFRI	黄 Yellow	179/179	AA	1/0
84	09南9-22 09nan9-22	ZFRI	白 White	177/177	AA	1/0
85	09南10-5 09nan10-5	ZFRI	白 White	177/179	AA	1/0
86	4-3-11	ZFRI	黄 Yellow	179/179	AA	1/0
87	5-3-7	ZFRI	黄 Yellow	179/179	AA	1/0
88	中油桃13号 Zhongyoutao 13	ZFRI	白 White	177/179	AA	1/0
89	春美 Chunmei	ZFRI	白 White	177/179	AA	1/0
90	中桃9号 Zhongtao 9	ZFRI	白 White	177/179	AA	1/0
91	中桃10号 Zhogntao 10	ZFRI	黄 Yellow	179/179	AA	1/0
92	08北-12-1 08bei-12-1	ZFRI	白 White	177/177	AA	1/0
93	双喜红 Shuangxihong	ZFRI	黄 Yellow	179/179	AA	1/0
94	中油20号 Zhognyou 20	ZFRI	白 White	177/179	AA	1/0
95	中桃红玉 Zhongtaohongyu	ZFRI	白 White	177/179	AA	1/0
96	06-3-113	ZFRI	黄 Yellow	179/179	AA	1/0
97	05-3-102	ZFRI	黄 Yellow	179/179	AA	1/0
98	晚油桃 Wanyoutao	ZFRI	黄 Yellow	179/179	AA	1/0
99	中油18号 Zhongyou 18	ZFRI	白 White	177/179	AA	1/0
100	1区井蟠 Yiqujingpan	ZFRI	白 White	177/179	AA	1/0
101	中桃22号 Zhongtao 22	ZFRI	白 White	177/179	AA	1/0
102	春瑞 Chunrui	ZFRI	白 White	177/179	AA	1/0
103	9-6-180	ZFRI	白 White	177/179	AA	1/0
104	04-7-13	ZFRI	白 White	177/179	AA	1/0
105	中桃白玉 Zhongtaobaiyu	ZFRI	白 White	177/177	AA	1/0
106	中油19号 Zhongyou 19	ZFRI	黄 Yellow	179/179	AA	1/0
107	中油27号 Zhongyou 27	ZFRI	白 White	177/179	AA	1/0
108	中油12号 Zhongyou 12	ZFRI	白 White	177/179	AA	1/0
109	4-1-6	ZFRI	黄 Yellow	177/179	AA	1/1
110	枣油桃 Zaoyoutao	ZFRI	白 White	177/179	AA	1/0
111	红不软 Hongburuan	ZFRI	白 White	177/177	AA	1/0
112	6-7-6	ZFRI	白 White	177/179	AA	1/0
113	13-33	ZFRI	白 White	177/179	AA	1/0
114	4-9-16	ZFRI	白 White	177/179	AA	1/0
115	小花红芒果 Xiaohuahongmangguo	ZFRI	黄 Yellow	179/179	AA	1/0
116	中桃8号 Zhongtao 8	ZFRI	白 White	177/179	AA	1/0
117	中油15号 Zhongyou 15	ZFRI	白 White	177/179	AA	1/0
118	红芒果 Hongmangguo	ZFRI	黄 Yellow	179/179	AA	1/0
119	中蟠2号 Zhongpan 2	ZFRI	黄 Yellow	179/179	AA	1/0
120	春蜜 Chunmi	ZFRI	白 White	177/179	AA	1/0
121	中农金辉 Zhongnongjinhui	ZFRI	黄 Yellow	179/179	AA	1/0
122	中桃5号 Zhongtao 5	ZFRI	白 White	177/179	AA	1/0

Table 1

Table 2

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Table 3

References 33

[1]	LAYNE D R, BASSI D. The Peach: Botany, Production and Uses . 2008:17.
[2]	ROBERTSON A J, HORVAT R J, LYON B G, MEREDITH F I, SENTER S D, OKIE W R. Comparison of quality characteristic of selected yellow and white-fleshed peach cultivar. Journal of Food Science, 1990,55:1308-1311.
[3]	ARANZANA M J, ILLA E, HOWAD W, ARÚS P. A first insight into peach [Prunus persica(L.) Batsch] SNP variability. Tree Genetics and Genomes, 2012,8:1359-1369. doi: 10.1007/s11295-012-0523-6
[4]	CONNORS C H. Some notes on the inheritance of unit characters in the peach. Proceedings of the American Society for Horticultural Sciences, 1920,16:24-36.
[5]	BRANDI F, BAR E, MOURGUES F, HORVATH G, TURCSI E, GIULIANO G, LIVERANI A, TARTARINI S, LEWINSOHN E, ROSATI C. Study of ‘Redhaven’ peach and its white-fleshed mutant suggests a key role of CCD4 carotenoid dioxygenase in carotenoid and norisoprenoid volatile metabolism. BMC Plant Biology, 2011,11:24. doi: 10.1186/1471-2229-11-24 pmid: 21269483
[6]	MA J J, LI J, ZHAO J B, ZHOU H, REN F, WANG L, GU C, LIAO L, HAN Y P. Inactivation of a gene encoding carotenoid cleavage dioxygenase (CCD4) leads to carotenoid-based yellow coloration of fruit flesh and leaf midvein in peach. Plant Molecular Biology Report, 2014,32:246-257.
[7]	WARBURTON M L, BECERRA-VELÀSQUEZ V L, GOFFREDA J C, BLISS F A. Utility of RAPD markers in identifying genetic linkages to genes of economic interest in peach. Theoretical and Applied Genetics, 1996,93:920-925. doi: 10.1007/BF00224094 pmid: 24162426
[8]	ARÚS P, VERDE I, SOSINSKI B, ZHEBENTYAYEVA T, ABBOTT A G. The peach genome. Tree Genetics and Genomes, 2012,8:531-547.
[9]	CANTÍN C M, GOGORCENA Y, MORENO M A. Phenotypic diversity and relationships of fruit quality traits in peach and nectarine [Prunus persica(L.) Batsch] breeding progenies. Euphytica, 2010,171:211.
[10]	俞明亮, 马瑞娟, 沈志军, 章镇. 桃果肉颜色、果皮茸毛和花粉育性性状的分子标记. 园艺学报, 2006,33(3):511-517.
	YU M L, MA R J, SHEN Z J, ZHANG Z. Molecular markers linked to specific characteristics of Prunus persica (L.) Batsch. Acta Horticulturae Sinica, 2006,33(3):511-517. (in Chinese)
[11]	ADAMI M, FRANCESCHI P D, BRANDI F, LIVERANI A, GIOVANNINI D, ROSATI C, DONDINI L, TARTARINI S. Identifying a carotenoid cleavage dioxygenase (CCD4) gene controlling yellow/white fruit flesh color of peach. Plant Molecular Biology Report, 2013,31:1166-1175.
[12]	FALCHI R, VENDRAMIN E, ZANON L, SCALABRIN S, CIPRIANI G, VERDE I, VIZZOTTO G, MORGANTE M. Three distinct mutational mechanisms acting on a single gene underpin the origin of yellow flesh in peach. The Plant Journal, 2013,76:175-187. doi: 10.1111/tpj.12283 pmid: 23855972
[13]	张南南, 牛良, 崔国朝, 潘磊, 曾文芳, 王志强, 鲁振华. 一种高通量提取桃DNA方法的建立与应用, 中国农业科学, 2018,51(13):2614-2621.
	ZHANG N N, NIU L, CUI G C, PAN L, ZENG W F, WANG Z Q, LU Z H. Establishment and application of a high-throughout protocol for peach (Prunus persica) DNA extraction. Scientia Agricultura Sinica, 2018,51(13):2614-2621. (in Chinese)
[14]	FUKAMATSU Y, TAMURA T, HIHARA S, ODA K. Mutations in the CCD4 carotenoid cleavage dioxygenase gene of yellow-flesh peaches. Bioscience, Biotechnology, and Biochemistry, 2013,77(12):2514-2516.
[15]	ARANZANA M J, ABBASSI E, HOWAD W, ARÚS P. Genetic variation, population structure and linkage disequilibrium in peach commercial varieties. BMC Genetics, 2010,11:69. doi: 10.1186/1471-2156-11-69 pmid: 20646280
[16]	VERDE I, ABBOTT A G, SCALABRIN S, JUNG S, SHU S Q, MARRONI F, ZHEBENTYAYEVA T, DETTORI M T, GRIMWOOD J, CATTONARO F, ZUCCOLO A, ROSSINI L, JENKINS J, VENDRAMIN E, MEISEL L A, DECROOCQ V, SOSINSKI B, PROCHNIK S, MITROS T, POLICRITI A, et al. The high-quality draft genome of peach (Prunus persica) identifies unique patterns of genetic diversity, domestication and genome evolution. Nature Genetics, 2013,45:487-494. doi: 10.1038/ng.2586 pmid: 23525075
[17]	FRESNEDO-RAMÍREZ J, FRETT T J, SANDEFUR P J, SALGADO- ROJAS A, CLARK J R, GASIC K, PEACE C P, ANDERSON N, HARTMANN T P, BYRNE D H, , BINK M C A M, , VAN DE WEG W E, CRISOSTO C H, GRADZIEL T M. QTL mapping and breeding value estimation through pedigree-based analysis of fruit size and weight in four diverse peach breeding programs. Tree Genetics and Genomes, 2016,12:25.
[18]	DARDICK C, CALLAHAN A, HORN R, RUIZ K B, ZHEBENTYAYEVA T, HOLLENDER C, WHITAKER M, ABBOTT A, SCORZA R. PpeTAC1 promotes the horizontal growth of branches in peach trees and is a member of a functionally conserved gene family found in diverse plants species. The Plant Journal, 2013,75:618-630. doi: 10.1111/tpj.12234 pmid: 23663106
[19]	HOLLENDER C A, PASCAL T, TABB A, HADIARTO T, SRINIVASAN C, WANG W P, LIU Z C, SCORZA R, DARDICK C. Loss of a highly conserved sterile alpha motif domain gene (WEEP) results in pendulous branch growth in peach trees. Proceedings of the National Academy of Sciences of the USA, 2018,115(20):E4690-E4699. doi: 10.1073/pnas.1704515115 pmid: 29712856
[20]	HOLLENDER C A, HADIARTO T, SRINIVASAN C, SCORZA R, DARDICK C. A brachytic dwarfism trait (dw) in peach trees is caused by a nonsense mutation within the gibberellic acid receptor PpeGID1c. New Phytologist, 2016,210:227-239. doi: 10.1111/nph.13772 pmid: 26639453
[21]	鲁振华, 牛良, 张南南, 姚家龙, 崔国朝, 曾文芳, 潘磊, 王志强. 基于SNP标记桃矮化基因的精细定位. 中国农业科学, 2017,50(18):3572-3580.
	LU Z H, NIU L, ZHANG N N, YAO J L, CUI G C, ZENG W F, PAN L, WANG Z Q. Fine mapping of dwarfing gene for peach based on SNP markers. Scientia Agricultura Sinica, 2017,50(18):3572-3580. (in Chinese)
[22]	GU C, WANG L, WANG W, ZHOU H, MA B Q, ZHENG H Y, FANG T, OGUTU C, VIMOLMANGKANG S, HAN Y P. Copy number variation of a gene cluster encoding endopolygalacturonase mediates flesh texture and stone adhesion in peach. Journal of Experimental Botany, 2016,67(6):1993-2005. pmid: 26850878
[23]	LÓPEZ-GIRONA E, ZHANG Y, EDUARDO I, MORA J R H, ALEXIOU K G, ARÚS P, ARANZANA M J. A deletion affecting an LRR-RLK gene co-segregates with the fruit flat shape trait in peach. Scientific Report, 2017,7:6714.
[24]	VENDRAMIN E, PEA G, DONDINI L, PACHECO I, DETTORI M T, GAZZA L, SCALABRIN S, STROZZI F, TARTARINI S, BASSI D, VERDE I, ROSSINI L. A unique mutation in a MYB gene cosegregates with the nectarine phenotype in peach. PLoS ONE, 2014,9(3):e90574. doi: 10.1371/journal.pone.0090574 pmid: 24595269
[25]	PAN L, ZENG W F, NIU L, LU Z H, WANG X B, LIU H, CUI G C, ZHU Y Q, CHU J F, LI W P, FANG W C, CAI Z G, LI G H, WANG Z Q. PpYUC11, a strong candidate gene for the stony hard phenotype in peach(Prunus persica L. Batsch), participates in IAA biosynthesis during fruit ripening. Journal of Experimental Botany, 2015,66(22):7031-7044. doi: 10.1093/jxb/erv400 pmid: 26307136
[26]	SHEN Z J, CONFOLENT C, LAMBERT P, POËSSEL J L, QUILOT-TURION B, YU M L, MA R J, PASCAL T. Characterization and genetic mapping of a new blood-flesh trait controlled by the single dominant locus DBF in peach. Tree Genetics and Genomes, 2013,9:1435-1446. doi: 10.1007/s11295-013-0649-1
[27]	LISCH D. How important are transposons for plant evolution? Nature Reviews Genetics, 2013,14:49-61. doi: 10.1038/nrg3374 pmid: 23247435
[28]	KOBAYASHI S, GOTO-YAMAMOTO N, HIROCHIKA H. Retrotransposon-induced mutations in grape skin color. Science, 2014,304(5673):982. doi: 10.1126/science.1095011 pmid: 15143274
[29]	ZHANG L Y, HU J, HAN X L, LI J J, GAO Y, RICHARDS C M, ZHANG C X, TIAN Y, LIU G M, GUL H, WANG D J, TIAN Y, YANG C X, MENG M H, YUAN G P, KANG G D, WU Y L, WANG K, ZHANG H T, WANG D P, CONG P H. A high-quality apple genome assembly reveals the association of a retrotransposon and red fruit colour. Nature Communication, 2019,10:1494.
[30]	BUTELLI E, LICCIARDELLO C, ZHANG Y, LIU J, MACKAY S, BAILEY P, REFORGIATO-RECUPERO G, MARTIN C. Retrotransposons control fruit-specific, cold-dependent accumulation of anthocyanins in blood oranges. The Plant Cell, 2012,24:1242-1255. doi: 10.1105/tpc.111.095232 pmid: 22427337
[31]	CHEN T, ZHANG Y D, ZHAO L, ZHU Z, LIN J, ZHANG S B, WANG C L. Fine mapping and candidate gene analysis of a green- revertible albino gene gra (t) in rice. Journal of Genetics and Genomics, 2009,36(2):117-123. doi: 10.1016/S1673-8527(08)60098-3 pmid: 19232310
[32]	LEE G, PIAO R H, LEE Y J, KIM B, SEO J H, LEE D Y, JANG S, JIN Z, LEE C S, CHIN J H, KOH H. Identification and characterization of large embryo, a new gene controlling embryo size in rice (Oryza sativa L.). Rice, 2019,12:22. doi: 10.1186/s12284-019-0277-y pmid: 30972509
[33]	FERREIRA D S, KEVEI Z, KUROWSKI T, FONSECA M E N, MOHAREB F, BOITEUX L S, THOMPSON A J. Bifurcate flower truss: a novel locus controlling inflorescence branching in tomato contains a defective MAP kinase gene. Journal of Experimental Botany, 2018,69(10):2581-2593. doi: 10.1093/jxb/ery076 pmid: 29509915

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

名称 Code	引物序列 Primer sequence	退火温度 Annealing temperature (℃)	片段长度 Size (bp)
CCD4-F	5'-ACCACCTGTTTGACGGAGAC-3'	55	594
CCD4-R	5'-TGCTCATGAAGAGCTTGCCA-3'
LTR-F（CCD4）	5'-TACCTGAGAGCTTCTCGTGC-3'	55	729
CCD4-SSR-F	5'- ROX -CCCATTTTGCAGTGAAGGGC-3'	55	177
CCD4-SSR-R	5'-GCTGTGGTGCTTTTGTGGAG-3'
CCD4-SNP-F	5′-GGGTGATCCAATGCCTAAGA-3′	55	510
CCD4-SNP-R	5′-GGCTCTCTAGCCACGAAAAA-3′

Molecular Marker-Assisted Identification of Yellow/White Flesh Trait for 122 Peach Cultivars (Lines)

RichHTML

PDF

Abstract

Cite this article

share this article

Figures/Tables 7

References 33

Related Articles 15

Metrics

Comments

Recommended 0

基因型 Genotype	品种（系） Cultivar (line)
CT插入 CT insertion	瑞光18号 Ruiguang 18
	金陵黄露 Jinlinghuanglu
	五月火 Mayfire
	瑞光22号 Ruiguang 22
	阿姆肯 Armking
	连黄 Lianhuang
	紫金红2号 Zijinghong 2
	弗雷德里克 Frederica
	金霞蟠桃Jinxiapantao
	瑞光28号 Ruiguang 28
	紫金红3号 Zijinhong 3
	金山早红 Jinshanzaohong
	黄金蜜1号 Huangjinmi 1
	4-3-11
	5-3-7
	中桃10号 Zhongtao 10
	双喜红 Shuangxihong
	06-3-113
	05-3-102
	晚油桃 Wanyoutao
	中油19号 Zhongyou 19
	小花红芒果 Xiaohuahongmangguo
	红芒果 Hongmangguo
	中蟠2号 Zhongpan 2
	中农金辉 Zhongnongjinhui
LTR插入 LTR insertion	锦绣 Jinxiu
	连黄 Lianhuang
	金童9号 Babygold 9
	金晖 Jinhui
	丰黄 Fenghuang
	早红港 Early Redhaven
	锦香 Jinxiang 金童8号 Babygold 8
CT重复+LTR插入 CT insertion + LTR insertion	金童7号 Babygold 7
	沪油003号 Huyou 003
	早金露 Zaojinlu
	金童5号 Babygold 5
	霞光 Xiaguang
	金童6号 Babygold 6
	04-7-13
CT插入+SNP替换 CT insertion +SNP subsitution	弗尔蒂尼莫蒂尼 Fertilia Morettini

[1]	LI FeiFei, LIAN XueFei, YIN Tao, CHANG YuanYuan, JIN Yan, MA XiaoChuan, CHEN YueWen, YE Li, LI YunSong, LU XiaoPeng. The Relationship Between Mastication and Development of Segment Membranes in Citrus Fruits [J]. Scientia Agricultura Sinica, 2023, 56(2): 333-344.
[2]	YAN LeLe,BU LuLu,NIU Liang,ZENG WenFang,LU ZhenHua,CUI GuoChao,MIAO YuLe,PAN Lei,WANG ZhiQiang. Widely Targeted Metabolomics Analysis of the Effects of Myzus persicae Feeding on Prunus persica Secondary Metabolites [J]. Scientia Agricultura Sinica, 2022, 55(6): 1149-1158.
[3]	GUO ShaoLei,XU JianLan,WANG XiaoJun,SU ZiWen,ZHANG BinBin,MA RuiJuan,YU MingLiang. Genome-Wide Identification and Expression Analysis of XTH Gene Family in Peach Fruit During Storage [J]. Scientia Agricultura Sinica, 2022, 55(23): 4702-4716.
[4]	HAO Yan,LI XiaoYing,YE Mao,LIU YaTing,WANG TianYu,WANG HaiJing,ZHANG LiBin,XIAO Xiao,WU JunKai. Characteristics of Volatile Components in Peach Fruits of 21shiji and Jiucui and Their Hybrid Progenies [J]. Scientia Agricultura Sinica, 2022, 55(22): 4487-4499.
[5]	ZHU ChangWei,MENG WeiWei,SHI Ke,NIU RunZhi,JIANG GuiYing,SHEN FengMin,LIU Fang,LIU ShiLiang. The Characteristics of Soil Nutrients and Soil Enzyme Activities During Wheat Growth Stage Under Different Tillage Patterns [J]. Scientia Agricultura Sinica, 2022, 55(21): 4237-4251.
[6]	WEI JingJie,JIANG NingBo,LIANG Yan,ZHANG Qian,SUN YingJian,HU Ge. Effect of Matrine on NLRP3 Inflammasome Signaling Pathway in H9N2 AIV Infected Mice [J]. Scientia Agricultura Sinica, 2022, 55(21): 4315-4326.
[7]	XIA YuXin,LIANG Yan,WANG HaiYang,GUO MengLing,ZHOU Bu,DAI Xu,YANG ZhangPing,MAO YongJiang. Effects of the Number of Subclinical Mastitis and Somatic Cell Score in Milk of Parity 1 on Somatic Cell Score of Holstein Cows for Parity 2 [J]. Scientia Agricultura Sinica, 2022, 55(20): 4052-4064.
[8]	MengQi WANG,Na MI,Jing WANG,YuShu ZHANG,RuiPeng JI,NiNa CHEN,XiaXia LIU,Ying HAN,WangYiPu LI,JiaYing ZHANG. Simulation of Canopy Silking Dynamic and Kernel Number of Spring Maize Under Drought Stress [J]. Scientia Agricultura Sinica, 2022, 55(18): 3530-3542.
[9]	WANG LuWei,SHEN ZhiJun,LI HeHuan,PAN Lei,NIU Liang,CUI GuoChao,ZENG WenFang,WANG ZhiQiang,LU ZhenHua. Analysis of Genetic Diversity of 79 Cultivars Based on SSR Fluorescence Markers for Peach [J]. Scientia Agricultura Sinica, 2022, 55(15): 3002-3017.
[10]	SHEN ZhiJun, TIAN Yu, CAI ZhiXiang, XU ZiYuan, YAN Juan, SUN Meng, MA RuiJuan, YU MingLiang. Evaluation of Brown Rot Resistance in Peach Based on Genetic Resources Conserved in National Germplasm Repository of Peach in Nanjing [J]. Scientia Agricultura Sinica, 2022, 55(15): 3018-3028.
[11]	TAN FengLing,ZHAN Ping,WANG Peng,TIAN HongLei. Effects of Thermal Sterilization on Aroma Quality of Flat Peach Juice Based on Sensory Evaluation and GC-MS Combined with OPLS-DA [J]. Scientia Agricultura Sinica, 2022, 55(12): 2425-2435.
[12]	FANG TaoHong,ZHANG Min,MA ChunHua,ZHENG XiaoChen,TAN WenJing,TIAN Ran,YAN Qiong,ZHOU XinLi,LI Xin,YANG SuiZhuang,HUANG KeBing,WANG JianFeng,HAN DeJun,WANG XiaoJie,KANG ZhenSheng. Application of Yr52 Gene in Wheat Improvement for Stripe Rust Resistance [J]. Scientia Agricultura Sinica, 2022, 55(11): 2077-2091.
[13]	LI Ang,MIAO YuLe,MENG JunRen,NIU Liang,PAN Lei,LU ZhenHua,CUI GuoChao,WANG ZhiQiang,ZENG WenFang. Peptidome Analysis of Mesocarp in Melting Flesh and Stony Hard Peach During Fruit Ripening [J]. Scientia Agricultura Sinica, 2022, 55(11): 2202-2213.
[14]	HAN Xiao, YANG HangYu, CHEN WeiKai, WANG Jun, HE Fei. Effects of Different Rootstocks on Flavonoids of Vitis vinifera L. cv. Tannat Grape Fruits [J]. Scientia Agricultura Sinica, 2022, 55(10): 2013-2025.
[15]	ZHANG YuanYuan,LIU WenJing,ZHANG BinBin,CAI ZhiXiang,SONG HongFeng,YU MingLiang,MA RuiJuan. Characterization of the Lactone Volatile Compounds in Different Types of Peach (Prunus persica L.) Fruit and Evaluations of Their Contributions to Fruit Overall Aroma [J]. Scientia Agricultura Sinica, 2022, 55(10): 2026-2037.