Scientia Agricultura Sinica ›› 2021, Vol. 54 ›› Issue (7): 1455-1468.doi: 10.3864/j.issn.0578-1752.2021.07.011

• EFFICIENT UTILIZATION OF FERTILIZER AND WATER • Previous Articles     Next Articles

Analysis of Ammonium Uptake and Growth Differences of Rice Varieties with Different Nitrogen Recovery Efficiency at Seedling Stage

HUANG Xiu,YE Chang,YAN JinXiang,LI FuMing,CHU Guang,XU ChunMei,CHEN Song,ZHANG XiuFu,WANG DanYing()   

  1. China National Rice Research Institute/State Key Laboratory of Rice Biology, Hangzhou 311400
  • Received:2020-03-23 Accepted:2020-05-29 Online:2021-04-01 Published:2021-04-22
  • Contact: DanYing WANG E-mail:wangdanying@caas.cn

RichHTML

43

PDF

280

Abstract:

【Objective】The aim of this study was to determine the response of different N recovery efficiency (NRE) varieties to external NH4 +concentration, and to explain the mechanism of N uptake difference among rice varieties.【Method】A high-NRE (QL) and a low-NRE (HK) rice variety were cultivated hydroponically at different NH4 +concentrations, and the varietal difference of NH4 + absorption at seedling stage were compared by analysis of the root NH4 + absorption rate at the low NH4 + concentration of 0-0.80 mmol·L -1 and the high NH4 + concentration of 1.00-12.96 mmol·L -1, and by calculating the NH4 + absorption kinetics parameter Vmax and Km; The difference of seedling growth between varieties were analyzed by comparing the plant height, tiller number, chlorophyll content, dry matter and N accumulation of aboveground and underground part of rice seedlings growing in different NH4 +concentrations, root morphology including total root length, root volume, root surface area, average root diameter, and root tip number were analyzed using the root scanning instrument; The root net fluxes of NH4 + were measured non-invasively using SIET (scanning ion-selective electrode technique), root respiration was measured using a Hansatech oxygen electrode and an oxygraph control system.【Result】(1) In the low NH4 + concentration range of 0-0.8 mmol·L -1, the uptake of NH4 + by QL and HK seedlings stage were in accordance with Michaelich-Menten equation, and the Vmax of high-NRE variety QL was 1.66 times higher than that of low-NRE variety HK. In the NH4 + concentration higher than 1 mmol·L -1, the uptake of NH4 + by rice seedlings kept increasing with the increase of NH4 +concentration, and the uptake rate of NH4 + by QL was greater than that of HK at the same NH4 +level. (2) The root meristematic zone of both varieties showed a net NH4 +influx, while it increased with the increase of NH4 + concentration, and the high-NRE variety QL had greater value than low-NRE variety HK by 42.0%, 71.8% and 63.6% at LN, MN and HN, respectively; The net NH4 +efflux in the root elongation zone was showed at LN and HN in the low-NRE variety HK, while it was only showed at HN in the high-NRE variety QL, and 34.30% lower than variety HK. (3) Although an appropriate increase of NH4 + could increase the plant height, tiller number, leaf chlorophyll content, dry matter and N accumulations, while HN inhibited plant growth, especially root growth. The growth of high-NRE variety QL did not show an advantage compared with low-NRE variety HK in the treatment of LN and MN, however, QL had greater tillering rate and dry matter accumulation rate by 65.7% and 31.4% compared with HK during 10-20 days after seeding in HN, respectively. Although the N concentration of root of variety QL was 15.1% lower than that of variety HK, it’s aboveground N accumulation was 23.5% greater, which indicated that QL was more efficient than HK in transporting the N absorbed by roots to the aboveground. 【Conclusion】 It was concluded that compared with the low-NRE variety, the high-NRE variety had more NH4 + transport carriers on the cell membrane of the root system, and the NH4 + absorbed by the root system had a fast assimilation and transport speed, as well as a high tiller rate and dry matter accumulation rate in the seedling stage.

Key words: rice, N absorption efficiency, ammonium absorption, NH4 + circulation, seedling morphology

Fig. 1

Uptake kinetics of NH4+ in rice roots at seedling stage (DAS16) (2016)DAS: Days after seeding. The same as below"

Table 1

The kinetic parameters of NH4+ uptake in high affinity transport system at seedling stage (DAS 16) (2016)"

品种 Variety 拟合曲线 Matched curve Vmax Km R2
HK y=0.0272x+0.0982 10.18 0.277 0.984**
QL y=0.0256x+0.0591 16.92 0.433 0.995**

Fig. 2

Net NH4+ flux in the root meristematic (A) and elongation (B) regions of two varieties supplied with different N levels (DAS20) (2016)Different lowercase letters represent significant differences between different nitrogen treatments and varieties (P<0.05). The same as below"

Fig. 3

Leaf color, tiller development and dry matter accumulation of two varieties supplied with different N levels (2016)DAT: Days after transplanting. The same as below"

Fig. 4

Plant height, leaf color, and tiller capacity of two varieties supplied with different N levelsThe data is the average of the seedling experiments in 2016 and 2018"

Fig. 5

Dry matter accumulation of two varieties supplied with different N levelsThe data is the average of the seedling experiments in 2016 and 2018"

Fig. 6

Root morphology of two varieties supplied with different N levels (2018)"

Fig. 7

Root O2 consumption of two varieties supplied with different N levels (DAS20) (2018)"

Fig. 8

Shoot and root N concentration of two varieties supplied with different N levelsThe data is the average of the seedling experiments in 2016 and 2018"

Fig. 9

Shoot, root and total plant N accumulation of two varieties supplied with different N levelsThe data is the average of the seedling experiments in 2016 and 2018"

[1] MALCOLM J H, SIMON G . Exploiting genetic variation in nitrogen use efficiency for cereal crop improvement. Current Opinion in Plant Biology, 2019,49:35-42.
[2] 栗方亮, 李忠佩, 刘明, 江春玉. 氮素浓度和水分对水稻土硝化作用和微生物特性的影响. 中国生态农业学报, 2012,20(9):1113-1118.
LI F L, LI Z P, LIU M, JIANG C Y. Effects of nitrogen concentration and moisture on nitrification and microbial characteristics of rice soil. Chinese Journal of Ecological Agriculture, 2012,20(9):1113-1118. (in Chinese)
[3] WANG M Y, SIDDIQI M Y, RUTH T J . Glass ADM ammonium uptake by rice roots. II. Kinetics of 13NH4 + influx across the plasmalemma . Plant Physiology, 1993,103(4):1259-1267.
[4] 尹西翔. 水稻氮高效品种的筛选及其机理的初步探讨[D]. 武汉: 华中农业大学, 2005.
YIN X X. Selection of nitrogen efficient rice varieties and preliminary discussion on their mechanism[D]. Wuhan: Huazhong Agricultural University, 2005. (in Chinese)
[5] 叶全宝, 张洪程, 魏海燕, 张瑛, 汪本福, 夏科, 霍中洋, 戴其根, 许轲. 不同土壤及氮肥条件下水稻氮利用效率和增产效应研究. 作物学报, 2005,31(11):38-44.
YE Q B, ZHANG H C, WEI H Y, ZHANG Y, WANG B F, XIA K, HUO Z Y, DAI Q G, XU K. Study on nitrogen utilization efficiency and yield increasing effect of rice under different soil and nitrogen fertilizer conditions. Acta Agronomica Sinica, 2005,31(11):38-44. (in Chinese)
[6] 于飞, 施卫明. 近10年中国大陆主要粮食作物氮肥利用率分析. 土壤学报, 2015,52(6):117-130.
YU F, SHI W M. Analysis on nitrogen utilization efficiency of major grain crops in mainland China in recent 10 years. Acta Pedologica Sinica, 2015,52(6):117-130. (in Chinese)
[7] CHEN Y T, HOU Y, CUI K H, WANG J, FAHAD S, ZHANG C D, NIE L X, PENG J, HUANG J L, FU P H, PENG S B . Crop management based on multi-split topdressing enhances grain yield and nitrogen use efficiency in irrigated rice in China. Field Crops Research, 2015,184:50-57.
[8] DING W C, XU X P, HE P, ULLAH S, ZHANG J J, CUI Z L, ZHOU W . Improving yield and nitrogen use efficiency through alternative fertilization options for rice in China: A meta-analysis. Field Crops Research, 2018,227:11-18.
[9] 江琳琳, 赵晗舒, 杨武, 赵明辉, 范淑秀, 曹英丽, 任彬彬, 马殿荣. 水稻氮利用效率评价及其与产量性状的关系. 沈阳农业大学学报, 2019,50(6):641-647.
JIANG L L, ZHAO H S, YANG W, ZHAO M H, FAN S X, CAO Y L, REN B B, MA D R. Evaluation of nitrogen use efficiency of rice and its relationship with yield traits. Journal of Shenyang Agricultural University, 2019,50(6):641-647. (in Chinese)
[10] RAKOTOSONA T, DUSSERRE J, LETOURMY P, RAMONTA I R, CAO T V, RAMANANTSONAIRINA A, POUMET P, AHMADI N, RABOIN L M . Genetic variability of nitrogen use efficiency in rainfed upland rice. Field Crops Research, 2017,213:194-203.
[11] LON S, TAKAYASU W, SHUNJI I . Behavior of N within rice plants, comparing between japonica and indica varieties. Ecology and Environment Sciences, 2004,l3(4):646-650.
[12] SAMONT S O P, WILSON L T, MEDLEY J C, PINSON S R M, MCCIUNG A M, LALES J S . Nitrogen utilization efficiency: Relationships with grain yield, grain protein, and yield‐related traits in rice. Agronomy Journal, 2006,98(1):168-176.
[13] 张传胜, 龙银成, 周娟, 董桂春, 王余龙. 不同产量类型籼稻品种氮素吸收利用特征的研究. 扬州大学学报, 2004(2):17-21.
ZHANG C S, LONG Y C, ZHOU J, DONG G C, WANG Y L. Study on nitrogen uptake and utilization characteristics of indica rice varieties with different yield types. Journal of Yangzhou University, 2004(2):17-21. (in Chinese)
[14] 李娜, 杨志远, 代邹, 孙永健, 徐徽, 何艳, 蒋明今, 严田蓉, 郭长春, 马均. 水氮管理对不同氮效率水稻根系性状、氮素吸收利用及产量的影响. 中国水稻科学, 2017,31(5):500-512.
LI N, YANG Z Y, DAI Z, SUN Y J, XU W, HE Y, JIANG M J, YAN T R, GUO C C, MA J. Effects of water and nitrogen management on root traits, nitrogen accumulation and utilization and yield of rice with different nitrogen efficiencies. Chinese Journal of Rice Science, 2017,31(5):500-512. (in Chinese).
[15] CHU G, CHEN S, XU C M, WANG D Y, ZHANG X F . Agronomic and physiological performance of indica/japonica hybrid rice cultivar under low nitrogen conditions. Field Crops Research, 2019,243:107625.
[16] WANG F, PENG S B . Yield potential and nitrogen use efficiency of China’s super rice. Journal of Integrative Agriculture, 2017,16(5):1000-1008.
[17] 张振华, 刘强, 宋海星, 荣湘民 , ISMAIL A M. 水稻生长、根系生理特性和ABA含量的基因型差异与耐盐性的关系. 植物营养与肥料学报, 2011,17(5):1035-1043.
ZHANG Z H, LIU Q, SONG H X, RONG X M, ISMAIL A M. Relationship between genotype differences in growth, physiological characteristics of roots and ABA content and salinity tolerance in rice. Journal of Plant Nutrition and Fertilizers, 2011,17(5):1035-1043. (in Chinese)
[18] ZHANG H, XUE Y G, WANG Z Q, YANG J C, ZHANG J H . Morphological and physiological traits of roots and their relationships with shoot growth in “super” rice. Field Crops Research, 2009,113(1):31-40.
[19] 戢林, 李廷轩, 张锡洲, 余海英. 水稻氮高效基因型根系分泌物中有机酸和氨基酸的变化特征. 植物营养与肥料学报, 2012,18(5):1046-1055.
JI L, LI T X, ZHANG X Z, YU H Y. Changes of organic acids and amino acids in nitrogen efficient rice root exudates. Journal of Plant Nutrition and Fertilizers, 2012,18(5):1046-1055. (in Chinese)
[20] 叶利庭, 吕华军, 宋文静, 图尔迪, 沈其荣, 张亚丽. 不同氮效率水稻生育后期氮代谢酶活性的变化特征. 土壤学报, 2011,48(1):134-142.
YE L T, LÜ H J, SONG W J, TU E D, SHEN Q R, ZHANG Y L. Change characteristics of nitrogen metabolic enzyme activity in late growth period of rice with different nitrogen efficiency. Acta Pedologica Sinica, 2011,48(1):134-142. (in Chinese)
[21] CHEN G, GUO S W, KRONZUCKER H J, SHI W M . Nitrogen use efficiency (NUE) in rice links to NH4 + toxicity and futile NH4 + cycling in roots. Plant and Soil , 2013,369(1/2):351-363.
[22] 张晓果. 高氮肥用量下水稻根系铵冗余吸收及其与氮肥利用效率的关系[D]. 北京: 中国农业科学院, 2016.
ZHANG X G. The relationship between ammonium redundancy absorption in rice root system and nitrogen utilization efficiency under high nitrogen fertilizer dosage[D]. Beijing: Chinese Academy of Agricultural Sciences, 2016. (in Chinese)
[23] 刘婷, 尚忠林. 植物对铵态氮的吸收转运调控机制研究进展. 植物生理学报, 2016,52(6):799-809.
LIU T, SHANG Z L. Advances in the research on the regulation mechanism of plant absorption and transport of ammonium nitrogen. Journal of Plant Physiology, 2016,52(6):799-809. (in Chinese)
[24] BRITTO D T, KRONZUCKER H J . NH4 + toxicity in higher plants: A critical review . Journal of Plant Physiology, 2002,159(6):567-584.
[25] LI Q, LI B H, KRONZUCKER H J, SHI W M . Root growth inhibition by NH4 + in Arabidopsis is mediated by the root tip and is linked to NH4 +efflux and GMPase activity. Plant, Cell & Environment, 2010,33(9):1529-1542.
[26] BRITTO D T, KRONZUCKER H J . Constancy of nitrogen turnover kinetics in the plant cell: Insights into the integration of subcellular N fluxes. Planta, 2001,213(2):175-181.
[27] 张晓果, 徐春梅, 陈松, 计成林, 章秀福, 王丹英. 氮利用效率差异水稻品种的根系铵离子吸收特性. 中国农业科学, 2015,48(22):4428-4436.
ZHANG X G, XU C M, CHEN S, JI C L, ZHANG X F, WANG D Y. Absorption characteristics of root ammonium ion in different rice varieties with different nitrogen utilization efficiency. Scientia Agricultura Sinica, 2015,48(22):4428-4436. (in Chinese)
[28] 封克, 孙小茗, 汪晓丽, 盛海君. 铵对不同作物根系钾高亲和转运系统的影响. 植物营养与肥料学报, 2007,13(5):877-881.
FENG K, SUN X M, WANG X L, SHENG H J. Effect of ammonium on potassium affinity transport system in root system of different crops. Journal of Plant Nutrition and Fertilizers, 2007,13(5):877-881. (in Chinese)
[29] 孙小茗, 封克, 汪晓丽. K + 高亲和转运系统吸收动力学特征及其受NH4 + 影响的研究 . 植物营养与肥料学报, 2007,13(2):208-212.
SUN X M, FENG K, WANG X L. Characteristics of absorption kinetics and its influence on NH4 + in K + highly affinity transport systems . Journal of Plant Nutrition and Fertilizers, 2007,13(2):208-212. (in Chinese)
[30] WANG D Y, XU C M, YAN J X, ZHANG X G, CHEN S, CHAUHAN B S, WANG L, ZHANG X F . 15N tracer-based analysis of genotypic differences in the uptake and partitioning of N applied at different growth stages in transplanted rice . Field Crops Research, 2017,211:27-36.
[31] WANG D Y, XU C M, YE C, CHEN S, CHU G, ZHANG X F . Low recovery efficiency of basal fertilizer-N in plants does not indicate high basal fertilizer-N loss from split-applied N in transplanted rice. Field Crops Research, 2018,229:8-16.
[32] 杨肖娥, 孙羲. 不同水稻品种对低氮反应的差异及其机制的研究. 土壤学报, 1992(1):73-79.
YANG X E, SUN X. Study on the difference and mechanism of response of different rice varieties to low nitrogen. Acta Pedologica Sinica, 1992(1):73-79. (in Chinese)
[33] LYNCH J . Root architecture and plant productivity. Plant Physiology, 1995,109(1):7-13.
[34] FAGERIA N K, BALIGAR C, CLARK R B . Micronutrients in crop production. Advances in Agronomy, 2002,77:185-268.
[35] KRONZUCKER H J, BRITTO D T, DAVENPORT R J, TESTER M . Ammonium toxicity and the real cost of transport. Trends in Plant Science, 2001,6(8):335-337.
[36] 武厥彬. 水稻叶片的奥秘与高产栽培. 农村科技, 1999(9):10.
WU J B. The mystery of rice leaves and high-yield cultivation. Rural Science and Technology, 1999(9):10. (in Chinese)
[37] TANAKA A, GARCIA C V, DIEM N T . Studies of the relationship between tillering and nitrogen uptake of the rice plant. Soil Science and Plant Nutrition, 1965,11(1):9-13.
[1] XIAO DeShun, XU ChunMei, WANG DanYing, ZHANG XiuFu, CHEN Song, CHU Guang, LIU YuanHui. Effects of Rhizosphere Oxygen Environment on Phosphorus Uptake of Rice Seedlings and Its Physiological Mechanisms in Hydroponic Condition [J]. Scientia Agricultura Sinica, 2023, 56(2): 236-248.
[2] ZHANG XiaoLi, TAO Wei, GAO GuoQing, CHEN Lei, GUO Hui, ZHANG Hua, TANG MaoYan, LIANG TianFeng. Effects of Direct Seeding Cultivation Method on Growth Stage, Lodging Resistance and Yield Benefit of Double-Cropping Early Rice [J]. Scientia Agricultura Sinica, 2023, 56(2): 249-263.
[3] ZHANG Wei,YAN LingLing,FU ZhiQiang,XU Ying,GUO HuiJuan,ZHOU MengYao,LONG Pan. Effects of Sowing Date on Yield of Double Cropping Rice and Utilization Efficiency of Light and Heat Energy in Hunan Province [J]. Scientia Agricultura Sinica, 2023, 56(1): 31-45.
[4] FENG XiangQian,YIN Min,WANG MengJia,MA HengYu,CHU Guang,LIU YuanHui,XU ChunMei,ZHANG XiuFu,ZHANG YunBo,WANG DanYing,CHEN Song. Effects of Meteorological Factors on Quality of Late Japonica Rice During Late Season Grain Filling Stage Under ‘Early Indica and Late Japonica’ Cultivation Pattern in Southern China [J]. Scientia Agricultura Sinica, 2023, 56(1): 46-63.
[5] SANG ShiFei,CAO MengYu,WANG YaNan,WANG JunYi,SUN XiaoHan,ZHANG WenLing,JI ShengDong. Research Progress of Nitrogen Efficiency Related Genes in Rice [J]. Scientia Agricultura Sinica, 2022, 55(8): 1479-1491.
[6] GUI RunFei,WANG ZaiMan,PAN ShengGang,ZHANG MingHua,TANG XiangRu,MO ZhaoWen. Effects of Nitrogen-Reducing Side Deep Application of Liquid Fertilizer at Tillering Stage on Yield and Nitrogen Utilization of Fragrant Rice [J]. Scientia Agricultura Sinica, 2022, 55(8): 1529-1545.
[7] LIAO Ping,MENG Yi,WENG WenAn,HUANG Shan,ZENG YongJun,ZHANG HongCheng. Effects of Hybrid Rice on Grain Yield and Nitrogen Use Efficiency: A Meta-Analysis [J]. Scientia Agricultura Sinica, 2022, 55(8): 1546-1556.
[8] HAN XiaoTong,YANG BaoJun,LI SuXuan,LIAO FuBing,LIU ShuHua,TANG Jian,YAO Qing. Intelligent Forecasting Method of Rice Sheath Blight Based on Images [J]. Scientia Agricultura Sinica, 2022, 55(8): 1557-1567.
[9] GAO JiaRui,FANG ShengZhi,ZHANG YuLing,AN Jing,YU Na,ZOU HongTao. Characteristics of Organic Nitrogen Mineralization in Paddy Soil with Different Reclamation Years in Black Soil of Northeast China [J]. Scientia Agricultura Sinica, 2022, 55(8): 1579-1588.
[10] ZHU DaWei,ZHANG LinPing,CHEN MingXue,FANG ChangYun,YU YongHong,ZHENG XiaoLong,SHAO YaFang. Characteristics of High-Quality Rice Varieties and Taste Sensory Evaluation Values in China [J]. Scientia Agricultura Sinica, 2022, 55(7): 1271-1283.
[11] ZHAO Ling, ZHANG Yong, WEI XiaoDong, LIANG WenHua, ZHAO ChunFang, ZHOU LiHui, YAO Shu, WANG CaiLin, ZHANG YaDong. Mapping of QTLs for Chlorophyll Content in Flag Leaves of Rice on High-Density Bin Map [J]. Scientia Agricultura Sinica, 2022, 55(5): 825-836.
[12] JIANG JingJing,ZHOU TianYang,WEI ChenHua,WU JiaNing,ZHANG Hao,LIU LiJun,WANG ZhiQin,GU JunFei,YANG JianChang. Effects of Crop Management Practices on Grain Quality of Superior and Inferior Spikelets of Super Rice [J]. Scientia Agricultura Sinica, 2022, 55(5): 874-889.
[13] ZHANG YaLing, GAO Qing, ZHAO Yuhan, LIU Rui, FU Zhongju, LI Xue, SUN Yujia, JIN XueHui. Evaluation of Rice Blast Resistance and Genetic Structure Analysis of Rice Germplasm in Heilongjiang Province [J]. Scientia Agricultura Sinica, 2022, 55(4): 625-640.
[14] WANG YaLiang,ZHU DeFeng,CHEN RuoXia,FANG WenYing,WANG JingQing,XIANG Jing,CHEN HuiZhe,ZHANG YuPing,CHEN JiangHua. Beneficial Effects of Precision Drill Sowing with Low Seeding Rates in Machine Transplanting for Hybrid Rice to Improve Population Uniformity and Yield [J]. Scientia Agricultura Sinica, 2022, 55(4): 666-679.
[15] CHEN TingTing, FU WeiMeng, YU Jing, FENG BaoHua, LI GuangYan, FU GuanFu, TAO LongXing. The Photosynthesis Characteristics of Colored Rice Leaves and Its Relation with Antioxidant Capacity and Anthocyanin Content [J]. Scientia Agricultura Sinica, 2022, 55(3): 467-478.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd