不同氮吸收效率水稻品种的苗期铵吸收特性及生长差异分析

doi:10.3864/j.issn.0578-1752.2021.07.011

Abstract

Abstract:

【Objective】The aim of this study was to determine the response of different N recovery efficiency (NRE) varieties to external NH₄ ⁺concentration, and to explain the mechanism of N uptake difference among rice varieties.【Method】A high-NRE (QL) and a low-NRE (HK) rice variety were cultivated hydroponically at different NH₄ ⁺concentrations, and the varietal difference of NH₄ ⁺ absorption at seedling stage were compared by analysis of the root NH₄ ⁺ absorption rate at the low NH₄ ⁺ concentration of 0-0.80 mmol·L ^-1 and the high NH₄ ⁺ concentration of 1.00-12.96 mmol·L ^-1, and by calculating the NH₄ ⁺ absorption kinetics parameter V_max and K_m; The difference of seedling growth between varieties were analyzed by comparing the plant height, tiller number, chlorophyll content, dry matter and N accumulation of aboveground and underground part of rice seedlings growing in different NH₄ ⁺concentrations, root morphology including total root length, root volume, root surface area, average root diameter, and root tip number were analyzed using the root scanning instrument; The root net fluxes of NH₄ ⁺ were measured non-invasively using SIET (scanning ion-selective electrode technique), root respiration was measured using a Hansatech oxygen electrode and an oxygraph control system.【Result】(1) In the low NH₄ ⁺ concentration range of 0-0.8 mmol·L ^-1, the uptake of NH₄ ⁺ by QL and HK seedlings stage were in accordance with Michaelich-Menten equation, and the V_max of high-NRE variety QL was 1.66 times higher than that of low-NRE variety HK. In the NH₄ ⁺ concentration higher than 1 mmol·L ^-1, the uptake of NH₄ ⁺ by rice seedlings kept increasing with the increase of NH₄ ⁺concentration, and the uptake rate of NH₄ ⁺ by QL was greater than that of HK at the same NH₄ ⁺level. (2) The root meristematic zone of both varieties showed a net NH₄ ⁺influx, while it increased with the increase of NH₄ ⁺ concentration, and the high-NRE variety QL had greater value than low-NRE variety HK by 42.0%, 71.8% and 63.6% at LN, MN and HN, respectively; The net NH₄ ⁺efflux in the root elongation zone was showed at LN and HN in the low-NRE variety HK, while it was only showed at HN in the high-NRE variety QL, and 34.30% lower than variety HK. (3) Although an appropriate increase of NH₄ ⁺ could increase the plant height, tiller number, leaf chlorophyll content, dry matter and N accumulations, while HN inhibited plant growth, especially root growth. The growth of high-NRE variety QL did not show an advantage compared with low-NRE variety HK in the treatment of LN and MN, however, QL had greater tillering rate and dry matter accumulation rate by 65.7% and 31.4% compared with HK during 10-20 days after seeding in HN, respectively. Although the N concentration of root of variety QL was 15.1% lower than that of variety HK, it’s aboveground N accumulation was 23.5% greater, which indicated that QL was more efficient than HK in transporting the N absorbed by roots to the aboveground. 【Conclusion】 It was concluded that compared with the low-NRE variety, the high-NRE variety had more NH₄ ⁺ transport carriers on the cell membrane of the root system, and the NH₄ ⁺ absorbed by the root system had a fast assimilation and transport speed, as well as a high tiller rate and dry matter accumulation rate in the seedling stage.

Key words: rice, N absorption efficiency, ammonium absorption, NH₄ ⁺ circulation, seedling morphology

HUANG Xiu,YE Chang,YAN JinXiang,LI FuMing,CHU Guang,XU ChunMei,CHEN Song,ZHANG XiuFu,WANG DanYing. Analysis of Ammonium Uptake and Growth Differences of Rice Varieties with Different Nitrogen Recovery Efficiency at Seedling Stage[J].Scientia Agricultura Sinica, 2021, 54(7): 1455-1468.

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品种 Variety	拟合曲线 Matched curve	V_max	K_m	R²
HK	y=0.0272x+0.0982	10.18	0.277	0.984**
QL	y=0.0256x+0.0591	16.92	0.433	0.995**

Analysis of Ammonium Uptake and Growth Differences of Rice Varieties with Different Nitrogen Recovery Efficiency at Seedling Stage

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