龙眼SPL基因家族全基因组鉴定及表达分析

doi:10.3864/j.issn.0578-1752.2020.20.014

Abstract

Abstract:

【Objective】To provide a reference for the study of the function of SPL in Dimocarpus longan growth and development, all members of the longan SPL(DlSPL) gene family were identified and their expression patterns were analyzed in this study.【Method】The DlSPL family members, with their basic physicochemical properties, gene structures, phylogenetic relationships, and cis-acting promoter elements, were identified by bioinformatics analysis, and their expression patterns in non-embryonic callus (NEC), embryogenic culture and embryonic callus (EC) under different hormone treatments were analyzed by FPKM and qRT-PCR technology. 【Result】The results showed that there were 14 DlSPL gene family members, and their gene and protein structures were all highly conserved and contained only one SBP domain. The promoters of DlSPL genes had a large number of light response elements, stress response elements, hormone response elements, tissue specific regulatory elements and cis-regulatory elements related to plant growth and development, indicating that DlSPL genes might be regulated by light, hormone, stress and other factors. RNA-seq expression analysis showed that 11 of 14 DlSPL members were detected in EC under the different light qualities and different hormones treatments, and only DlSPL8 showed a down-regulated expression trend under blue and white light treatments, and the expression levels of DlSPL3 and DlSPL13 members were higher in EC under 2, 4-D+KT treatment than that of under 2, 4-D and KT treatment, respectively; 13 DlSPLs were detected in non-embryogenic and embryogenic cultures, seven members of them had the highest expression levels in the NEC stage. QRT-PCR results showed that DlSPL1 and DlSPL14 had the highest expression levels in the EC stage, DlSPL5, DlSPL7 and DlSPL13 had the highest expression levels in the incomplete embryonic compact structure (ICpEC) stage; DlSPL1, DlSPL5, DlSPL7, and DlSPL13 were down-regulated under abscisic acid treatments. DlSPL5 was up-regulated under methyl jasmonate acid treatments, while the other three members were down-regulated. 【Conclusion】 A total of 14 longan SPL gene family members contained a highly conserved SBP domain were identified, and the DlSPL gene family might play an important role in longan somatic embryos, and respond to abscisic acid and methyl jasmonate element treatments.

Key words: Dimocarpus longan, SPL, genome-wide identification, expression analysis

LU BaoShun,ZHU YongJing,ZHANG ShuTing,LÜ YuMeng,LI XiaoFei,SONG YuYang,LAI ZhongXiong,LIN YuLing. Whole-Genome Identification and Expression Analysis of SPL Gene Family in Dimocarpus Longan[J].Scientia Agricultura Sinica, 2020, 53(20): 4259-4270.

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References 40

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