黄瓜单性结实性状遗传与QTL定位

doi:10.3864/j.issn.0578-1752.2020.01.015

Abstract

Abstract:

【Objective】 Parthenocarpy is an important trait affecting both yield and quality in the protected cucumber production. Genetic analysis of parthenocarpy and its QTL mapping are of great significance for improving breeding efficiency in cucumber.【Method】 Based on three-year phenotypic data, the genetic linkage map of cucumber was constructed by using SSR molecular markers obtained from cucumber genome sequencing, and the QTL-Seq analysis was used to map the parthenocarpy of cucumber, using recombinant inbred line F_2:8 constructed from strong parthenocarpic inbred line ‘6457’ and weak parthenocarpic inbred line ‘6426’.【Result】 The inheritance of parthenocarpy in cucumber accorded with quantitative inheritance. A genetic map containing 11 linkage groups was constructed using SSR markers, covering 555.0 cM of the genome with an average distance of 6.8 cM. In the spring of 2016, 2017 and 2018, a single QTL locus related to parthenocarpy was commonly detected between SSR19430 and SSR15419 markers (3.33-5.57 Mb) on chromosome 3, and the genetic distance was 6.6 cM, with 11%, 12.5% and 6.3% contribution rate, respectively. By QTL-Seq analysis, four QTLs related to parthenocarpy were identified on chromosomes 1 (4.38-11.00 Mb), 3 (2.24-10.66 Mb) and 6 (15.67-17.93 Mb; 26.33-27.49 Mb), the QTL on chromosome 3 overlapped with that detected by Map QTL approach. Csa3G047740, Csa3G073810, Csa3G043910 and Csa6G362930 were proposed to be candidate genes associated with parthenocarpic trait in cucumber. 【Conclusion】Four QTLs were identified on chromosome 1, 3 and 6, including one QTL on chromosome 3 that was consistently detected over years with relatively high contribution. The results could facilitate marker-assisted selection and understanding the underlying mechanism of parthenocarpy in cucumber.

Key words: cucumber, parthenocarpy, genetic map, QTL mapping

ZhiHong NIU,XiaoFei SONG,XiaoLi LI,XiaoYu GUO,ShuQiang HE,LuanJingZhi HE,ZhiHong FENG,ChengZhen SUN,LiYing YAN. Inheritance and QTL Mapping for Parthenocarpy in Cucumber[J].Scientia Agricultura Sinica, 2020, 53(1): 160-171.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2020.01.015

https://www.chinaagrisci.com/EN/Y2020/V53/I1/160

Figures/Tables 8

Fig. 1

Fig. 2

Fig. 3

Table 1

Table 2

Fig. 4

Fig. 5

Table 3

Candidate genes prediction and function annotations for parthenocarpy in cucumber"

基因号 Gene ID	变异类型 Variant	位置 Position	参考基因组基因型/该位点其他基因型Ref/Alt	拟南芥库里功能注释 Functional annotation of Arabidopsis
Csa1G042220	Nonsynonymous	4159708	T/C	编码ASPG1,与抗旱相关Encodes ASPG1 (ASPARTIC PROTEASE IN GUARD CELL 1). Functions in drought avoidance through abscisic acid (ABA) signalling in guard cells
Csa1G049980	Nonsynonymous	5680530	A/G	肽酶C78,泛素折叠修饰特异性肽酶1/2 Peptidase C78, ubiquitin fold modifier-specific peptidase 1/2
Csa6G367130	Nonsynonymous	16841983	C/G	靶圈套卷曲螺旋结构域蛋白 Target SNARE coiled-coil domain protein
Csa1G042830	Upstream	4532431	A/G	钙依赖性脂质结合（CALB结构域）家族蛋白 Calcium-dependent lipid-binding (CaLB domain) family protein
Csa1G045620	Upstream	5048161	T/C	编码钙依赖性蛋白激酶 Target SNARE coiled-coil domain protein
Csa1G045620	Upstream	5048182	T/C	编码钙依赖性蛋白激酶 Target SNARE coiled-coil domain protein
Csa1G045620	Upstream	5048193	G/A	编码钙依赖性蛋白激酶 Target SNARE coiled-coil domain protein
Csa1G045620	Upstream	5048246	G/A	编码钙依赖性蛋白激酶 Target SNARE coiled-coil domain protein
Csa3G002320	Upstream	239366	G/C	RB1诱导的卷曲螺旋蛋白 RB1-inducible coiled-coil protein
Csa3G036410	Upstream	2537701	C/T	富含亮氨酸的重复蛋白激酶家族蛋白 Leucine-rich repeat protein kinase family protein
Csa3G043910	Upstream	3022472	A/G	GRAS家族转录因子 GRAS family transcription factor
Csa3G043910	Upstream	3022485	T/A	GRAS家族转录因子 GRAS family transcription factor
Csa3G045170	Upstream	3188752	T/C	跨膜蛋白 Transmembrane protein
Csa3G047740	Upstream	3294577	C/T	咖啡酸/5-羟阿魏酸O-甲基转移酶 A caffeic acid/5-hydroxyferulic acid O-methyltransferase
Csa3G047740	Upstream	3294578	G/A	咖啡酸/5-羟阿魏酸O-甲基转移酶 A caffeic acid/5-hydroxyferulic acid O-methyltransferase
Csa3G073810	Upstream	3855792	G/A	编码参与介导植物对发病机制的反应的蛋白质 Encodes a protein involved in mediating plant responses to pathogenesis
Csa3G144130	Upstream	9538626	G/A	编码与CBL相互作用蛋白激酶SNF1和SOS2具有相似性的丝氨酸/苏氨酸蛋白激酶 Encodes a serine/threonine protein kinase with similarities to CBL-interacting protein kinases, SNF1 and SOS2
Csa3G144130	Upstream	9538714	G/A	编码与CBL相互作用蛋白激酶SNF1和SOS2具有相似性的丝氨酸/苏氨酸蛋白激酶 Encodes a serine/threonine protein kinase with similarities to CBL-interacting protein kinases, SNF1 and SOS2
Csa6G324800,Csa6G324810	Upstream	15102611	C/G	编码与NAD相关的苹果酸脱氢酶活性的蛋白 Encodes a protein with NAD-dependent malate dehydrogenase activity, located in chloroplasts
Csa6G344310	Upstream	15681038	A/G	腺苷酸核苷酸水解酶类似结构域蛋白的激酶 Kinase with adenine nucleotide alpha hydrolases-like domain-containing protein
Csa6G344310	Upstream	15681043	T/C	腺苷酸核苷酸水解酶类似结构域蛋白的激酶 Kinase with adenine nucleotide alpha hydrolases-like domain-containing protein
Csa6G362930	Upstream	16350065	A/C	亚精胺合成酶 Encodes a spermine synthase
Csa6G375720	Upstream	16932936	T/C	抗病蛋白（CC-NBS-LRR类）家族 Disease resistance protein (CC-NBS-LRR class) family
Csa6G375720	Upstream	16932970	A/G	抗病蛋白（CC-NBS-LRR类）家族 Disease resistance protein (CC-NBS-LRR class) family
Csa6G396650	Upstream	17566966	C/T	编码一种调节细菌肽聚糖传感和对细菌感染免疫的赖氨酸基序蛋白 Encodes a lysin-motif protein mediating bacterial peptidoglycan sensing and immunity to bacterial infection
Csa6G513690	Upstream	26648579	A/C	编码参与苯丙氨酸生物合成的质体局部芳香族脱水酶 Encodes a plastid-localized arogenate dehydratase involved in phenylalanine biosynthesis

Table 3

References 40

[1]	陈学好, 曹碚生 . 黄瓜单性结实研究概况. 中国蔬菜, 1994(3):56-59.
	CHEN X H, CAO B S . Research on parthenocarpy in cucumber. China Vegetables, 1994(3):56-59. (in Chinese)
[2]	PIKE L M, PETERSON C E . Inheritance of parthenocarpy in the cucumber (Cucumis sativus L.). Euphytica, 1969,18(1):101-105.
[3]	DE PONTI O M B, GARRETSEN F . Inheritance of parthenocarpy in pickling cucumbers (Cucumis sativus L.) and linkage with other characters. Euphytica, 1976,25(1):633-642.
[4]	EL-SHAWAF I I S, BAKER L R . Inheritance of parthenocarpic yield in gynoecious pickling cucumber for once-over mechanical harvest by diallel analysis of six gynoecious lines. Journal of the American Society for Horticultural Science, 1981,106(3):359-364.
[5]	EL-SHAWAF I I S, BAKER L R . Combining ability and genetic variances of G×H F₁ hybrids for parthenocarpic yield in gynoecious pickling cucumber from once-over mechanical harvest. Journal of the American Society for Horticultural Science, 1981,106(3):365-370.
[6]	曹碚生, 陈学好, 徐强, 顾春山 . 黄瓜单性结实世代遗传效应的初步研究. 园艺学报, 1997,24(1):53-56.
	CAO B S, CHEN X H, XU Q, GU C S . The genetic effects of parthenocarpic generations of cucumber. Acta Horticulturae Sinica, 1997,24(1):53-56. (in Chinese)
[7]	SUN Z Y, LOWER R L, STAUB J E . Analysis of generation means and components of variance for parthenocarpy in cucumber (Cucumis sativus L.). Plant Breeding, 2006,125(3):277-280.
[8]	SUN Z Y, LOWER R L, STAUB J E . Variance component analysis of parthenocarpy in elite U.S. processing type cucumber (Cucumis sativus L.) lines. Euphytica, 2006,148(3):331-339.
[9]	闫立英, 娄丽娜, 娄群峰, 陈劲枫 . 全雌黄瓜单性结实性的遗传分析. 园艺学报, 2008,35(10):1441-1446.
	YAN L Y, LOU L N, LOU Q F, CHEN J F . Inheritance of parthenocarpy in gynoecious cucumber (Cucumis sativus L.). Acta Horticulturae Sinica, 2008,35(10):1441-1446. (in Chinese)
[10]	闫立英, 娄丽娜, 李晓丽, 娄群峰, 冯志红, 陈劲枫 . 雌雄同株黄瓜单性结实性遗传分析. 中国农业科学, 2010,43(6):1295-1301.
	YAN L Y, LOU L N, LI X L, LOU Q F, FENG Z H, CHEN J F . Inheritance of parthenocarpy in monoecious cucumber (Cucumis sativus L.). Scientia Agricultura Sinica, 2010,43(6):1295-1301. (in Chinese)
[11]	闫立英, 娄丽娜, 冯志红, 李晓丽, 娄群峰, 陈劲枫 . 雌雄同株黄瓜单性结实性主基因+多基因混合遗传分析. 西北植物学报, 2009,29(6):1122-1126.
	YAN L Y, LOU L N, FENG Z H, LI X L, LOU Q F, CHEN J F . Analysis on mixed major gene and polygene inheritance of parthenocarpy in monoecious cucumber (Cucumis sativus L.). Acta Botanica Boreali-Occidentalia Sinica, 2009,29(6):1122-1126. (in Chinese)
[12]	闫立英, 娄丽娜, 冯志红, 娄群峰, 李晓丽, 陈劲枫 . 不同生态环境下雌雄同株黄瓜单性结实性遗传的比较. 应用生态学报, 2010,21(1):61-66.
	YAN L Y, LOU L N, FENG Z H, LOU Q F, LI X L, CHEN J F . Inheritance of parthenocarpy in monoecious cucumber(Cucumis sativus L.) under different eco-environments. Chinese Journal of Applied Ecology, 2010,21(1):61-66. (in Chinese)
[13]	陈学好, 王佳, 徐强, 嵇怡, 梁国华 . 一个与黄瓜单性结实基因连锁的ISSR标记. 分子遗传育种, 2008,6(1):85-88.
	CHEN X H, WANG J, XU Q, JI Y, LIANG G H . An ISSR marker linked to the parthenocarpic gene of cucumber. Molecular Plant Breeding, 2008,6(1):85-88. (in Chinese)
[14]	闫立英 . 黄瓜单性结实性生理和遗传分析及分子标记研究[D]. 南京: 南京农业大学, 2009.
	YAN L Y . Studies on physiological and genetic analysis and molecular makers of parthenocarpy in cucumber (Cucumis sativus L.)[D]. Nanjing: Nanjing Agricultural College, 2009. (in Chinese)
[15]	SUN Z Y, STAUB J, CHUNG S M, E LOWER R L . Identification and comparative analysis of quantitative trait loci associated with parthenocarpy in processing cucumber. Plant Breeding, 2006,125(3):281-287.
[16]	LIETZOW C D, ZHU H Y, PANDEY S, HAVEY M J, WENG Y Q . QTL mapping of parthenocarpic fruit set in North American processing cucumber. Theoretical and Applied Genetics, 2016,129(12):2387-2401.
[17]	武喆, 李蕾, 张婷, 张停林, 李季, 娄群峰, 陈劲枫 . 黄瓜单性结实性状的QTL定位. 中国农业科学, 2015,48(1):112-119.
	WU Z, LI L, ZHANG T, ZHANG T L, LI J, LOU Q F, CHEN J F . QTL mapping of parthenocarpy traits in cucumber. Scientia Agricultura Sinica, 2015,48(1):112-119. (in Chinese)
[18]	LI J, WU Z, CUI L, ZHANG T L, GUO Q W, XU J, JIA L, LOU Q F, HUANG S W, LI Z G, CHEN J F . Transcriptome comparison of global distinctive features between pollination and parthenocarpic fruit set reveals transcriptional phytohormone cross-talk in cucumber (Cucumis sativus L.). Plant Cell Physiology, 2014,55(7):1325-1342.
[19]	张婷 . 黄瓜单性结实相关基因CsWDR36的克隆与表达研究[D]. 南京: 南京农业大学, 2016.
	ZHANG T . Cloning and expression analysis of parthenocarpy related genes CsWDR36 in cucumber[D]. Nanjing: Nanjing Agricultural College, 2016. (in Chinese)
[20]	张婷, 武喆, 张开京, 徐建, 娄群峰, 李季, 陈劲枫 . 黄瓜单性结实候选基因预测与表达分析. 核农学报, 2016,30(2):224-230.
	ZHANG T, WU Z, ZHANG K J, XU J, LOU Q F, LI J, CHEN J F . Prediction and expression analysis of parthenocarpy candidate genes in cucumber. Journal of Nuclear Agricultural Sciences, 2016,30(2):224-230. (in Chinese)
[21]	李锡香, 朱德蔚 . 黄瓜种质资源描述规范和数据标准. 北京: 中国农业出版社, 2005.
	LI X X, ZHU D W. Descriptors and Data Standard of Cucumber (Cucumis sativus L.). Beijing: China Agricultural Press, 2005. (in Chinese)
[22]	MURRAY H G, THOMPSON W F . Rapid isolation of higher weight DNA. Nucleic Acids Research, 1980,8(19):4321.
[23]	HUANG S W, LI R Q, ZHANG Z H, LI L, GU X F, FAN W, LUCAS W J, WANG X W, XIE B Y, NI P X, REN Y, ZHU H M, LI J, LIN K, JIN W W, FEI Z J, LI G C, STAUB J B, KILIAN A VAN DER VOSSEN E A G , et al. The genome of the cucumber, Cucumis sativus L. Nature Genetics, 2009,41(12):1275-1281.
[24]	TAKAGI H, ABE A, YOSHIDA K, KOSUGI S, NATSUME S, MITSUOKA C, UEMURA A, UTSUSHI H, TAMIRU M, TAKUNO S, INNAN H, CANO L M, KAMOUN S, TERAUCHI R . QTL-seq: rapid mapping of quantitative trait loci in rice by whole genome resequencing of DNA from two bulked populations. Plant Journal, 2013,74(1):174-183.
[25]	LI H, HEAME S, BANZIGER M, LI Z, WANG J . Statistical properties of QTL linkage mapping in biparental genetic populations. Heredity, 2010,105(3):257-267.
[26]	李慧慧, 张鲁燕, 王健康 . 数量性状基因定位研究中若干常见问题的分析与解答. 作物学报, 2010,36(6):918-931.
	LI H H, ZHANG L Y, WANG J K . Analysis and answers to frequently asked questions in quantitative trait locus mapping. Acta Agronomica Sinica, 2010,36(6):918-931. (in Chinese)
[27]	YU H H, XIE W B, WANG J, XING Y Z, XU C G, LI X H, XIAO J H, ZHANG Q F . Gains in QTL detection using an ultra-high density SNP map based on population sequencing relative to traditional RFLP/SSR markers. PLoS ONE, 2011,6(3):e17595.
[28]	LI H, HEAME S, BANZIGER M, LI Z, WANG J . Statistical properties of QTL linkage mapping in biparental genetic populations. Heredity, 2010,105(3):257-267.
[29]	ZHOU Z Q, ZHANG C S, ZHOU Y, HAO Z F, WANG Z H, ZENG X, DI H, LI M S, ZHANG D G, YONG H J, ZHANG S H, WENG J F, LI X H . Genetic dissection of maize plant architecture with an ultra-high density bin map based on recombinant inbred lines. BMC Genomics, 2016,17(1):178.
[30]	FLEET C M, SUN T P . A DELLAcate balance: the role of gibberellin in plant morphogenesis. Current Opinion in Plant Biology, 2005,8:77-85.
[31]	ZHAO B, LI H T, LI J J, WANG B, DAI C, WANG J, LIU K D . Brassica napus DS-3, encoding a DELLA protein, negatively regulates stem elongation through gibberellin signaling pathway. Theoretical and Applied Genetics, 2017,130(4):727-741.
[32]	LI W J, ZHANG J X, SUN H Y, WANG S M, CHEN K Q, LIU Y X, LI H, MA Y, ZHANG Z H . FveRGA1, encoding a DELLA protein, negatively regulates runner production in Fragaria vesca. Planta, 2018,247(4):941-951.
[33]	RAJEEV R, SAWHNEY V K . Polyamines and flower development in the male sterile stamenless-2 mutant of tomato (Lycopersicon esculentum Mill.). Plant Physiology, 1990,93:446-452.
[34]	COSTA G, BAGNI N . Effect of polyamines on fruit set of apple. HortScience, 1983,18(1):59-61.
[35]	徐继忠, 陈海江, 邵建柱, 袁小乱, 马宝焜, 章文才 . 外源多胺促进红富士苹果花芽形成的效应. 果树科学, 1998,15(1):10-12.
	XU J Z, CHEN H J, SHAO J Z, YUAN X L, MA B K, ZHANG W C . Effects of exogenous polyamines on flower bud formation of red Fuji apple variety. Journal of Fruit Sciences, 1998,15(1):10-12. (in Chinese)
[36]	徐继忠, 陈海江, 马宝焜, 章文才 . 外源多胺对富士苹果花和幼果内源多胺与激素的影响. 园艺学报, 2001,28(3):206-210.
	XU J Z, CHEN H J, MA B K, ZHANG W C . Effects of exogenous spermidine on the levels of endogenous hormones and polyamines in the flowers and fruitlets of red Fuji apple. Acta Horticulturae Sinica, 2001,28(3):206-210. (in Chinese)
[37]	张秋明, 郑玉生, 魏岳荣, 刘昆玉, 谢深喜 . 柑桔多胺代谢及其对生长结果调控的研究. 湖南农业大学学报, 2000,26(4):271-273.
	ZHANG Q M, ZHENG Y S, WEI Y R, LIU K Y, XIE S X . Studies on polyamine metabolism and its regulation of growth and fruit set in Citrus. Journal of Hunan Agricultural University, 2000,26(4):271-273. (in Chinese)
[38]	任小林, 马锋旺, 王飞 . 亚精胺对李果实乙烯的产生和呼吸速率的影响. 植物生理学通讯, 1995,31(3):186-188.
	REN X L, MA F W, WANG F . Effect of spermidine on ethylene and respiration of plum. Plant Physiology Communications, 1995,31(3):186-188. (in Chinese)
[39]	陈学好, 于杰, 徐强, 郭绍贵 . Spd和MGBG对黄瓜子房内源多胺和蛋白质组成的影响及与单性结实的关系. 园艺学报, 2005,32(4):632-637.
	CHEN X H, YU J, XU Q, GUO S G . Effects of Spd and MGBG on endogenous polyamine levels and protein profiles in ovary and its relationship with parthenocarpy of cucumber. Acta Horticulturae Sinica, 2005,32(4):632-637. (in Chinese)
[40]	于杰 . 黄瓜单性结实与多胺代谢的关系及多胺调控研究[D]. 扬州: 扬州大学, 2003.
	YU J . Study on the relationship between parthenocarpy and polyamines metabolism of cucumber ovary and exogenous regulation with polyamines[D]. Yangzhou: Yangzhou University, 2003. (in Chinese)

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

样品 Sample	原始数据 Raw base (bp)	有效数据 Clean base (bp)	有效率 Effective rate (%)	碱基错误率 Error rate (%)	Q_phred值 Q20 (%)	Q_phred值 Q30 (%)	GC含量 GC content (%)
P₁	5376170100	5069987400	94.30	0.03	96.68	92.68	38.92
np	8447689500	7672464900	88.11	0.04	93.99	87.01	39.84
P₂	4140457200	4004934600	96.73	0.03	96.88	93.12	39.20
par	6980472300	6819972600	97.70	0.03	95.03	89.38	38.80

样品类型 Sample	Reads比对数 Mapped reads	Reads总数 Total reads	比对率 Mapping rate (%)	样品平均覆盖深度 Average depth (X)	1X数据覆盖比率 Coverage at least 1X (%)	4X数据覆盖比率 Coverage at least 4X (%)
P₁	29754479	33799916	88.03	15.27	98.12	92.06
P₂	23533219	26699564	88.14	12.40	97.21	86.53
par	39918316	45466484	87.80	20.24	98.65	95.70
np	44530059	51149766	87.06	21.61	98.70	95.57

Inheritance and QTL Mapping for Parthenocarpy in Cucumber

RichHTML

PDF

Abstract

Cite this article

share this article

Figures/Tables 8

References 40

Related Articles 15

Metrics

Comments

Recommended 0

[1]	CHEN JiHao, ZHOU JieGuang, QU XiangRu, WANG SuRong, TANG HuaPing, JIANG Yun, TANG LiWei, $\boxed{\hbox{LAN XiuJin}}$, WEI YuMing, ZHOU JingZhong, MA Jian. Mapping and Analysis of QTL for Embryo Size-Related Traits in Tetraploid Wheat [J]. Scientia Agricultura Sinica, 2023, 56(2): 203-216.
[2]	LI QingLin,ZHANG WenTao,XU Hui,SUN JingJing. Metabolites Changes of Cucumber Xylem and Phloem Sap Under Low Phosphorus Stress [J]. Scientia Agricultura Sinica, 2022, 55(8): 1617-1629.
[3]	LIU Jin,HU JiaXiao,MA XiaoDing,CHEN Wu,LE Si,JO Sumin,CUI Di,ZHOU HuiYing,ZHANG LiNa,SHIN Dongjin,LI MaoMao,HAN LongZhi,YU LiQin. Construction of High Density Genetic Map for RIL Population and QTL Analysis of Heat Tolerance at Seedling Stage in Rice (Oryza sativa L.) [J]. Scientia Agricultura Sinica, 2022, 55(22): 4327-4341.
[4]	KANG Chen,ZHAO XueFang,LI YaDong,TIAN ZheJuan,WANG Peng,WU ZhiMing. Genome-Wide Identification and Analysis of CC-NBS-LRR Family in Response to Downy Mildew and Powdery Mildew in Cucumis sativus [J]. Scientia Agricultura Sinica, 2022, 55(19): 3751-3766.
[5]	LinHan ZOU,XinYing ZHOU,ZeYuan ZHANG,Rui YU,Meng YUAN,XiaoPeng SONG,JunTao JIAN,ChuanLiang ZHANG,DeJun HAN,QuanHao SONG. QTL Mapping of Thousand-Grain-Weight and Its Related Traits in Zhou 8425B × Xiaoyan 81 Population and Haplotype Analysis [J]. Scientia Agricultura Sinica, 2022, 55(18): 3473-3483.
[6]	CHANG LiGuo,HE KunHui,LIU JianChao. Mining of Genetic Locus of Maize Stay-Green Related Traits Under Multi-Environments [J]. Scientia Agricultura Sinica, 2022, 55(16): 3071-3081.
[7]	CHEN Xi,LIU YingJie,DONG YongHao,LIU JinYan,LI Wei,XU PengJun,ZANG Yun,REN GuangWei. Effects of CMV-Infected Tobacco on the Performance, Feeding and Host Selection Behavior of Myzus persicae [J]. Scientia Agricultura Sinica, 2021, 54(8): 1673-1683.
[8]	ZHANG YaDong,LIANG WenHua,HE Lei,ZHAO ChunFang,ZHU Zhen,CHEN Tao,ZHAO QingYong,ZHAO Ling,YAO Shu,ZHOU LiHui,LU Kai,WANG CaiLin. Construction of High-Density Genetic Map and QTL Analysis of Grain Shape in Rice RIL Population [J]. Scientia Agricultura Sinica, 2021, 54(24): 5163-5176.
[9]	WANG Yan,FAN BaoJie,CAO ZhiMin,ZHANG ZhiXiao,SU QiuZhu,WANG Shen,WANG XueQing,PENG XiuGuo,MEI Li,WU YuHua,LIU ShaoXing,TIAN ShengMin,XU JunJie,JIANG ChunZhi,WANG WeiJuan,LIU ChangYou,TIAN Jing. Quantitative Trait Locus Mapping of Bruchids Resistance Based on A Novel Genetic Linkage Map in Cowpea (Vigna unguiculata) [J]. Scientia Agricultura Sinica, 2021, 54(22): 4740-4749.
[10]	WANG Ling,CAI Yi,WANG GuiChao,WANG Di,SHENG YunYan. Specific Length Amplified Fragment (SFLA) Sequencing Mapping Construction and QTL Analysis of Fruit Related Traits in Muskmelon [J]. Scientia Agricultura Sinica, 2021, 54(19): 4196-4206.
[11]	QU KeXin,HAN Lu,XIE JianGuo,PAN WenJing,ZHANG ZeXin,XIN DaWei,LIU ChunYan,CHEN QingShan,QI ZhaoMing. Mapping QTL for Soybean Fatty Acid Composition Based on RIL and CSSL Population [J]. Scientia Agricultura Sinica, 2021, 54(15): 3168-3182.
[12]	WANG JunZheng,ZHANG Qi,GAO ZiXing,MA XueQiang,QU Feng,HU XiaoHui. Effects of Two Microbial Agents on Yield, Quality and Rhizosphere Environment of Autumn Cucumber Cultured in Organic Substrate [J]. Scientia Agricultura Sinica, 2021, 54(14): 3077-3087.
[13]	LI ZhengGang,NONG Yuan,TANG YaFei,SHE XiaoMan,YU Lin,LAN GuoBing,DENG MingGuang,HE ZiFu. Molecular Characteristic and Pathogenicity Analyses of Cucumber green mottle mosaic virus (CGMMV) Infecting Bottle Gourd in Lianzhou, Guangdong [J]. Scientia Agricultura Sinica, 2020, 53(5): 955-964.
[14]	YongCe CAO,ShuGuang LI,XinCao ZHANG,JieJie KONG,TuanJie ZHAO. Construction of Genetic Map and Mapping QTL for Flowering Time in A Summer Planting Soybean Recombinant Inbred Line Population [J]. Scientia Agricultura Sinica, 2020, 53(4): 683-694.
[15]	ZHANG Jian,YANG Jing,WANG Hao,LI DongXiu,YANG GuiLi,HUANG CuiHong,ZHOU DanHua,GUO Tao,CHEN ZhiQiang,WANG Hui. QTL Mapping for Grain Size Related Traits Based on a High-Density Map in Rice [J]. Scientia Agricultura Sinica, 2020, 53(2): 225-238.