长期施肥和灌溉对土壤细菌数量、多样性和群落结构的影响

doi:10.3864/j.issn.0578-1752.2018.02.009

Abstract

Abstract: 【Objective】This study was carried out to investigate the bacterial community in a wheat-maize rotation field during wheat growing season in northern China. The effects of long-term different fertilization and irrigation regimes on the abundance, diversity and composition of bacterial community were identified. It will provide evidences for further optimizing fertilization and irrigation managements, improving soil productivity, and maintaining soil microbial diversity.【Method】Based on a long-term fertilization and irrigation experiment carried out in Wuqiao Experimental Station of China Agricultural University, six different soil samples were collected after wheat was harvested, including chemical fertilization and no irrigation (CI0), chemical fertilization and irrigation at the jointing stage (CI1), chemical fertilization and irrigation at the jointing and filling stages (CI2), manure fertilization and no irrigation (MI0), manure fertilization and irrigation at the jointing stage (MI1), and manure fertilization and irrigation at the jointing and filling stages (MI2). The abundance, diversity and composition of the bacteria community in different soil samples were revealed by using real-time PCR and Illumina Miseq sequencing platform, targeting the 16S rRNA gene. Correlation analysis was carried out between soil properties and the bacteria community abundance, diversity and structure.【Result】Irrigation significantly increased soil moisture and soil pH, and manure fertilization significantly increased Total Organic Carbon (TOC) content compared with no irrigation and chemical fertilization treatments, respectively. The bacterial 16S rRNA gene copy numbers ranged from 4.34×10⁹ to 1.39×10¹⁰ g^-1 d.w.s in different treatments. Irrigation significantly increased the bacterial 16S rRNA gene copy number by 1.17-1.60 and 0.76-1.93 times in the chemical and manure fertilization treatments, respectively. The bacterial α diversity index results showed that irrigation but not fertilization significantly affected bacterial α diversity index. At the phylum level, thirty-nine phyla were obtained in all the treatments, among which Proteobacteria, Actinobacteria, Chloroflexi, Acidobacteria and Bacteroidetes were the predominant phyla, accounting for 77.22%-86.28% of the total reads. There were significant variations in relative abundance of Actinobacteria【Conclusion】Irrigation greatly altered the abundance, diversity, and structure of the bacterial community, while fertilization had a minor effect on the abundance and structure of the bacterial community, and soil moisture and soil pH were the potential environmental factors associated with the bacterial community variations. (11.09%-27.01%), Bacteroidetes (5.45%-12.13%) and Saccharibacteria (2.41%-3.77%) among different treatments. Irrigation significantly decreased the relative abundance of Actinobacteria and Saccharibacteria by 36.48%-48.03%, 22.17%-33.67% and 15.21%-45.54%, 13.40%-23.97% in the chemical and manure fertilization treatments, respectively. Hierarchical clustering and principal component analysis (PCA) results showed that both fertilization and irrigation managements affect the bacterial structure, and irrigation had a stronger effect than fertilization on the bacterial structure. In addition, there were significant correlations between soil moisture, soil pH, Total Nitrogen (TN) content, and TOC content and the abundance, α diversity index and structure of the bacterial community.

Key words: manure fertilizer, irrigation, bacterial community diversity, high-throughput sequencing, northern China

YANG YaDong, WANG ZhiMin, ZENG ZhaoHai. Effects of Long-Term Different Fertilization and Irrigation Managements on Soil Bacterial Abundance, Diversity and Composition[J].Scientia Agricultura Sinica, 2018, 51(2): 290-301.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2018.02.009

https://www.chinaagrisci.com/EN/Y2018/V51/I2/290

References

[1] NANNIPIERI P, ASCHER J, CECCHERINI M T, LANDI L, PIETRAMELLARA G, RENELLA G. Microbial diversity and soil functions. European Journal of Soil Science, 2003, 54(4): 655-670.

[2] KENNEDY A C, SMITH K L. Soil microbial diversity and the sustainability of agricultural soils. Plant and Soil, 1995, 170(1): 75-86.

[3] BRUSSAARD L, DE RUITER P C, BROWN G G. Soil biodiversity for agricultural sustainability. Agriculture, Ecosystems and Environment, 2007, 121(3): 233-244.

[4] 张玉铭, 张佳宝, 胡春胜, 李晓欣, 朱安宁. 华北太行山前平原农田土壤水分动态与氮素的淋溶损失. 土壤学报, 2006, 41(3): 17-25.

ZHANG Y M, ZHANG J B, HU C S, LI X X, ZHU A N. Nitrite leaching in wheat-maize rotation field in the North China Plain. Acta Pedologica Sinica, 2006, 41(3): 17-25. (in Chinese)

[5] ABBOTT L K, MURPHY D V. Soil Biological Fertility. Netherlands: Kluwer Academic Publishers, 2003.

[6] 袁红朝, 秦红灵, 刘守龙, 童成立, 魏文学, 吴金水. 长期施肥对红壤性水稻土细菌群落结构和数量的影响. 中国农业科学, 2011, 44(22): 4610-4617.

YUAN H Z, QIN H L, LIU S L, TONG C L, WEI W X, WU J S. Response of abundance and composition of the bacterial community to long-term fertilization in paddy soils. Scientia Agricultura Sinica, 2011, 44(22): 4610-4617. (in Chinese)

[7] 乔洁, 毕利东, 张卫建, 沈仁芳, 张斌, 胡锋, 刘艳丽. 长期施用化肥对红壤性水稻土中微生物生物量、活性及群落结构的影响. 土壤, 2007, 39(5): 772-776.

QIAO J, BI L D, ZHANG W J, SHEN R F, ZHANG B, HU F, LIU Y L. Effects of long-term chemical fertilization on soil microbial biomass, activity and community in paddy soil in red soil region of China. Soils, 2007, 39(5): 772-776. (in Chinese)

[8] 丁建莉, 姜昕, 关大伟, 马鸣超, 赵百锁, 周宝库, 曹凤明, 李力, 李俊. 东北黑土微生物群落对长期施肥及作物的响应. 中国农业科学, 2016, 49(22): 4408-4418.

DING J L, JIANG X, GUAN D W, MA M C, ZHAO B S, ZHOU B K, CAO F M, LI L, LI J. Responses of micropopulation in black soil of northeast China to long-term fertilization and crops. Scientia Agricultura Sinica, 2016, 49(22): 4408-4418. (in Chinese)

[9] BERTHRONG T, BUCKLEY D H, DRINKWATER L E. Agricultural management and labile carbon additions affect soil microbial community structure and interact with carbon and nitrogen cycling. Microbial Ecology, 2013, 66(1): 158-170.

[10] WEI D, YANG Q, ZHANG J Z, WANG S, CHEN X, ZHANG X, LI W Q. Bacterial communities structure and diversity in a black soil as affected by long-term fertilization. Pedosphere, 2008, 18(5): 582-592.

[11] MA H K, BAI G Y, SUN Y, KOSTENKO O, ZHU X, LIN S, RUAN W B, ZHAO N X, BEZEMER T M. Opposing effects of nitrogen and water addition on soil bacterial and fungal communities in the Inner Mongolia steppe: A field experiment. Applied Soil Ecology, 2016, 108: 128-135.

[12] PAUL K I, POLGLASE P J, O'CONNELL A M, CARLYLE J C, SMETHURST P J, KHANNA P K. Defining the relation between soil

water content and net nitrogen mineralization. European Journal of Soil Science, 2003, 54(1): 39-48.

[13] HAN X, WANG R, LIU J, WANG M, ZHOU J, GUO W. Effects of vegetation type on soil microbial community structure and catabolic diversity assessed by polyphasic methods in north China. Journal of Environmental Sciences, 2007, 19(10): 1228-1234.

[14] SHOKRALLA S, SPALL J L, GIBSON J F, HAJIBABAEI M. Next-generation sequencing technologies for environmental DNA research. Molecular Ecology, 2012, 21(8): 1794-1805.

[15] 孙瑞波, 郭熙盛, 王道中, 褚海燕. 长期施用化肥及秸秆还田对砂姜黑土细菌群落的影响. 微生物学通报, 2015, 42(10): 2049-2057.

SUN R B, GUO X S, WANG D Z, CHU H Y. The impact of long-term application of chemical fertilizers and straw returning on soil bacterial community. Microbiology China, 2015, 42(10): 2049-2057. (in Chinese)

[16] MCHUGH T A, SCHWARTZ E. Changes in plant community composition and reduced precipitation have limited effects on the structure of soil bacterial and fungal communities present in a semiarid grassland. Plant and Soil, 2015, 388(1/2): 175-186.

[17] SUN M, XIAO T F, NING Z P, XIAO E Z, SUN W M. Microbial community analysis in rice paddy soils irrigated by acid mine drainage contaminated water. Applied Microbiology and Biotechnology, 2015, 99(6): 2911-2922.

[18] 钱永, 张兆吉, 费宇红, 陈京生张凤娥, 王昭. 华北平原浅层地下水可持续利用潜力分析. 中国生态农业学报, 2014, 22(8): 890-897.

QIAN Y, ZHANG Z J, FEI Y H, CHEN J S, ZHANG F E, WANG Z. Sustainable exploitable potential of shallow groundwater in the North China Plain. Chinese Journal of Eco-Agriculture, 2014, 22(8): 890-897. (in Chinese)

[19] WU M N, QIN H L, CHEN Z, WU J S, WEI W X. Effect of long-term fertilization on bacterial composition in rice paddy soil. Biology and Fertility of Soils, 2011, 47(4): 397-405.

[20] 鲍士旦. 土壤农化分析. 北京: 中国农业出版社, 2000.

BAO S D. Soil and Agricultural Chemistry Analysis. Beijing: China Agriculture Press, 2000. (in Chinese)

[21] SUZUKI M T, TAYLOR LT, DELONG E F. Quantitative analysis of small-subunit rRNA genes in mixed microbial populations via 5′-nuclease assays. Applied and Environmental Microbiology, 2000, 66(11): 4605-4614.

[22] WALTER J, TANNOCK G W, TILSALATIMISJARVI A, RODTONG S, LOACH D M, MUNRO K, ALATOSSAVA T. Detection and identification of gastrointestinal Lactobacillus species by using denaturing gradient gel electrophoresis and species-specific PCR primers. Applied and Environmental Microbiology, 2000, 66(1): 297-303.

[23] MCBAIN A J, BARTOLO R G, CATRENICH C E, CHARBONNEAU D, LEDDER R G, RICKARD A H, SYMMONS S A, GILBERT P. Microbial characterization of biofilms in domestic drains and the establishment of stable biofilm microcosms. Applied and Environmental Microbiology, 2003, 69(1): 177-185.

[24] FADROSH DW, MA B, GAJER P, SENGAMALAY N, OTT S, BROTMAN R M, RAVEL J. An improved dual-indexing approach for multiplexed 16S rRNA gene sequencing on the Illumina MiSeq platform. Microbiome, 2014, 2(1): 2-7.

[25] EDGAR R C. UPARSE: highly accurate OTU sequences from microbial amplicon reads. Nature methods, 2013, 10(10): 996-998.

[26] WANG Q, GARRITY G M, TIEDJE J M, COLE J R. Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Applied and Environmental Microbiology, 2007, 73(16): 5261-5267.

[27] SCHLOSS P D, GEVERS D, WESTCOTT S L. Reducing the effects of PCR amplification and sequencing artifacts on 16S rRNA-based studies. PLoS ONE, 2011, 6(12): 1-14.

[28] SHEN J P, ZHANG L M, GUO J F, RAY J L, HE J Z. Impact of long-term fertilization practices on the abundance and composition of soil bacterial communities in northeast China. Applied Soil Ecology, 2010, 46(1): 119-124.

[29] CHEN X, ZHANG L M, SHEN J P, XU Z H, HE J Z. Soil type determines the abundance and community structure of ammonia- oxidizing bacteria and archaea in flooded paddy soils. Journal of Soils and Sediments, 2010, 10(8): 1510-1516.

[30] 刘方春, 邢尚军, 马海林, 陈波, 丁延芹, 杜秉海. 持续干旱对樱桃根际土壤细菌数量及结构多样性影响. 生态学报, 2014, 34(3): 642-649．
LIU F C, XING S J, MA H L, CHEN B, DING Y Q, DU B H. Effects of continuous drought on soil bacteria populations and community diversity in sweet cherry rhizosphere. Acta Ecologica Sinica, 2014, 34(3): 642-649. (in Chinese)

[31] 徐永刚, 宇万太, 马强, 周桦. 长期不同施肥制度对潮棕壤微生物生物量碳、氮及细菌群落结构的影响. 应用生态学报, 2010, 21(8): 2078-2085.

XU Y G, YU W T, MA Q, ZHOU H. Effects of long-term fertilizations on microbial biomass C and N and bacterial community structure in an aquic brown soil. Chinese Journal of Applied Ecology, 2010, 21(8): 2078-2085. (in Chinese)

[32] 王伏伟, 王晓波, 李金才, 叶爱华, 王妍, 车威, 朱林. 施肥及秸秆还田对砂姜黑土细菌群落的影响. 中国生态农业学报, 2015, 23(10): 1302-1311.
WANG F W, WANG X B, LI J C, YE A H, WANG Y, CHE W, ZHU L. Effects of fertilization and straw incorporation on bacterial communities in lime concretion black soil. Chinese Journal of Eco-Agriculture, 2015, 23(10): 1302-1311. (in Chinese)

[33] LIU J J, SUI Y Y, YU Z H, SHI Y, CHU H Y, JIN J, LIU X B, WANG G H. High throughput sequencing analysis of biogeographical distribution of bacterial communities in the black soils of northeast China. Soil Biology and Biochemistry, 2014, 70: 113-122.

[34] GIRVAN M S, CAMPBEL C D, KILLHAM K, PROSSER J I, GLOVER L A. Bacterial diversity promotes community stability and functional resilience after perturbation. Environmental Microbiology, 2005, 7(3): 301-313.

[35] CHU H Y, FIERER N, LAUBER, C L, CAPORASO J G, KNIGHT R, GROGAN P. Soil bacterial diversity in the Arctic is not fundamentally different from that found in other biomes. Environmental Microbiology, 2010, 12(11): 2998-3006.

[36] LAUBER C L, STRIKLAND M S, BRADFORD M A, FIERER N. The influence of soil properties on the structure of bacterial and fungal communities across land-use types. Soil Biology and Biochemistry, 2008, 40(9): 2407-2415.

[37] 侯海军, 张文钊, 沈建林, 王聪秦红灵. 水分管理对稻田细菌丰度与群落结构的影响. 生态环境学报 2016, 25(9): 1431-1438.
HOU H J, ZHANG W Z, SHEN J L, WANG C, QIN H L. Effect of water management on soil bacterial abundance and community in the rice paddy field. Ecology and Environmental Sciences, 2016, 25(9): 1431-1438. (in Chinese)

[38] ZHOU J, GUAN D, ZHOU B, ZHAO B, MA M, QIN J, JIANG X, CHEN S, CAO F, SHEN D, LI J. Influence of 34-years of fertilization on bacterial communities in an intensively cultivated black soil in northeast China. Soil Biology and Biochemistry, 2015, 90: 42-51.

Related Articles 15

[1]	ZHANG KeKun,CHEN KeQin,LI WanPing,QIAO HaoRong,ZHANG JunXia,LIU FengZhi,FANG YuLin,WANG HaiBo. Effects of Irrigation Amount on Berry Development and Aroma Components Accumulation of Shine Muscat Grape in Root-Restricted Cultivation [J]. Scientia Agricultura Sinica, 2023, 56(1): 129-143.
[2]	WANG YangYang,LIU WanDai,HE Li,REN DeChao,DUAN JianZhao,HU Xin,GUO TianCai,WANG YongHua,FENG Wei. Evaluation of Low Temperature Freezing Injury in Winter Wheat and Difference Analysis of Water Effect Based on Multivariate Statistical Analysis [J]. Scientia Agricultura Sinica, 2022, 55(7): 1301-1318.
[3]	GOU ZhiWen,YIN Wen,CHAI Qiang,FAN ZhiLong,HU FaLong,ZHAO Cai,YU AiZhong,FAN Hong. Analysis of Sustainability of Multiple Cropping Green Manure in Wheat-Maize Intercropping After Wheat Harvested in Arid Irrigation Areas [J]. Scientia Agricultura Sinica, 2022, 55(7): 1319-1331.
[4]	ZHANG JiaHua,YANG HengShan,ZHANG YuQin,LI CongFeng,ZHANG RuiFu,TAI JiCheng,ZHOU YangChen. Effects of Different Drip Irrigation Modes on Starch Accumulation and Activities of Starch Synthesis-Related Enzyme of Spring Maize Grain in Northeast China [J]. Scientia Agricultura Sinica, 2022, 55(7): 1332-1345.
[5]	ZHAO XiaoHui,ZHANG YanYan,RONG YaSi,DUAN JianZhao,HE Li,LIU WanDai,GUO TianCai,FENG Wei. Study on Critical Nitrogen Dilution Model of Winter Wheat Spike Organs Under Different Water and Nitrogen Conditions [J]. Scientia Agricultura Sinica, 2022, 55(17): 3321-3333.
[6]	WANG QiaoJuan,HE Hong,LI Liang,ZHANG Chao,CAI HuanJie. Research on Soybean Irrigation Schedule Based on AquaCrop Model [J]. Scientia Agricultura Sinica, 2022, 55(17): 3365-3379.
[7]	MA XueMeng,YU ChengMin,SAI XiaoLing,LIU Zhen,SANG HaiYang,CUI BaiMing. PSORA: A Strategy Based on High-Throughput Sequence for Analysis of T-DNA Insertion Sites [J]. Scientia Agricultura Sinica, 2022, 55(15): 2875-2882.
[8]	WEI Lei,MI XiaoTian,SUN LiQian,LI ZhaoMin,SHI Mei,HE Gang,WANG ZhaoHui. Current Status of Chemical Fertilizers, Pesticides, and Irrigation Water and Their Reducing Potentials in Wheat Production of Northern China [J]. Scientia Agricultura Sinica, 2022, 55(13): 2584-2597.
[9]	MENG Yi,WENG WenAn,CHEN Le,HU Qun,XING ZhiPeng,WEI HaiYan,GAO Hui,HUANG Shan,LIAO Ping,ZHANG HongCheng. Effects of Water-Saving Irrigation on Grain Yield and Quality: A Meta-Analysis [J]. Scientia Agricultura Sinica, 2022, 55(11): 2121-2134.
[10]	CAO XiaoChuang,WU LongLong,ZHU ChunQuan,ZHU LianFeng,KONG YaLi,LU RuoHui,KONG HaiMin,HU ZhaoPing,DAI Feng,ZHANG JunHua,JIN QianYu. Effects of Different Irrigation and Nitrogen Application Regimes on the Yield, Nitrogen Utilization of Rice and Nitrogen Transformation in Paddy Soil [J]. Scientia Agricultura Sinica, 2021, 54(7): 1482-1498.
[11]	XIONG RuoYu,XIE JiaXin,TAN XueMing,YANG TaoTao,PAN XiaoHua,ZENG YongJun,SHI QingHua,ZHANG Jun,CAI Shuo,ZENG YanHua. Effects of Irrigation Management on Grain Yield and Quality of High-Quality Eating Late-Season Indica Rice in South China [J]. Scientia Agricultura Sinica, 2021, 54(7): 1512-1524.
[12]	LiYuan ZHANG,JinDong LÜ,XinYue SHI,Na YU,HongTao ZOU,YuLing ZHANG,YuLong ZHANG. Effects of Irrigation Regimes on N₂O and NO Emissions from Greenhouse Soil [J]. Scientia Agricultura Sinica, 2021, 54(5): 992-1002.
[13]	LEI HaoJie,LI GuiChun,KE HuaDong,WEI Lai,DING WuHan,XU Chi,LI Hu. Analysis of Impacts and Regulation Differences on Soil N₂O Emissions from Two Typical Crop Systems Under Drip Irrigation and Fertilization [J]. Scientia Agricultura Sinica, 2021, 54(4): 768-779.
[14]	DU Yu,ZHU ZhiWei,WANG Jie,WANG XiuNa,JIANG HaiBin,FAN YuanChan,FAN XiaoXue,CHEN HuaZhi,LONG Qi,CAI ZongBing,XIONG CuiLing,ZHENG YanZhen,FU ZhongMin,CHEN DaFu,GUO Rui. Construction and Annotation of Ascosphaera apis Full-Length Transcriptome Utilizing Nanopore Third-Generation Long-Read Sequencing Technology [J]. Scientia Agricultura Sinica, 2021, 54(4): 864-876.
[15]	SHAO MeiQi,ZHAO WeiSong,SU ZhenHe,DONG LiHong,GUO QingGang,MA Ping. Effect of Bacillus subtilis NCD-2 on the Growth of Tomato and the Microbial Community Structure of Rhizosphere Soil Under Salt Stress [J]. Scientia Agricultura Sinica, 2021, 54(21): 4573-4584.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

Effects of Long-Term Different Fertilization and Irrigation Managements on Soil Bacterial Abundance, Diversity and Composition

RichHTML

PDF

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0