Scientia Agricultura Sinica ›› 2017, Vol. 50 ›› Issue (21): 4071-4086.doi: 10.3864/j.issn.0578-1752.2017.21.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Tapetal Programmed Cell Death, Antioxidant Response and Oxidative Stress in Wheat Anthers Associated with D2-type Cytoplasmic Male-sterility

LIU ZiHan, SHI XiaoYi, YAN PengJiao, DUAN Yang, GENG XingXia, YE JiaLi, LI Sha, YANG XueTong, ZHANG GaoMing, JIA YuLin, ZHANG LingLi, SONG XiYue   

  1. College of Agronomy, Northwest A&F University, Yangling 712100, Shaanxi
  • Received:2017-04-10 Online:2017-11-01 Published:2017-11-01

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Abstract: 【Objective】D2 type cytoplasm is the considerable source of cytoplasm of cytoplasmic male sterility (CMS). It is of great value to further study the cytological characteristics and mechanism of physiology and molecules in heterologous cytoplasmic male sterility (CMS) wheat for the heterosis utilization. 【Method】To elucidate the abortive cytological characteristics of D2-type cytoplasmic male-sterility, the phenotypes of anthers, microspores, tapetum were analyzed by scanning electron microscope, DAPI staining, and paraffin section, as well as detectingtapetal apoptotic DNA fragmentation by DNA ladder. To further clarify that the D2-type cytoplasmic male-sterility is associated with abnormal tapetal programmed cell death and reactive oxygen species metabolism, the physiological properties, including the contents of reactive oxygen species (ROS), activities of ROS-scavenging enzymes (superoxide dismutase (SOD), peroxidase, catalase (CAT), ascorbate peroxidase (APX), and glutathione peroxidase (GPX)) and nonenzymatic antioxidants (ascorbic acid (AsA) and glutathione (GSH)), and the expression levels of antioxidant-associated genes from three D2-types of cytoplasmic male sterile lines (Va87B1-706A, C687B1-706A, Ju87B1-706A) and the maintainer 706B of wheat were compared.【Result】Compared with the maintainer line, at the trinucleate stage, the inner epidermal cells of D2-type cytoplasmic male-sterility accumulated less and more unconsolidated Ubisch bodies and the epidermis of microspores were extremely scabrous. The abortive pivotal period of pollen was initiated at the later uninucleate stage, which the delayed degradation of tapetum and abnormal structure of the microspore were related to the serious oxidative stress, disorganized activities of antioxidant enzymes, down-regulated contents of non-enzymatic antioxidants and significant difference of the expression level of some related enzyme genes in the three D2-types of cytoplasmic male sterile lines. Additionally, the expression level of SOD and APX genes was approximated to the activities of enzymes. However, there existed a distinct difference between the expression level of CAT gene and the activities of enzymes resulting from multiple-gene encoding CAT gene.【Conclusion】It was deduced that delay of start-up of the tapetal PCD (Programmed Cell Death) results from excessive ROS accumulation, and then destroyed the balance of the antioxidant defense system, and eventually led to the abortion of D2-types of cytoplasmic male sterile lines. Therefore, in order to further explore the abortive mechanism of cytoplasmic male sterility (CMS) wheat, this study provided an important theoretical basis and application significance.

Key words: wheat (Triticum aestivum L.), D2-types cytoplasmic male sterility, tapetal programmed cell death, ROS metabolism, gene expression

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