Scientia Agricultura Sinica ›› 2017, Vol. 50 ›› Issue (19): 3665-3677.doi: 10.3864/j.issn.0578-1752.2017.19.004

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Differential Proteomics Analysis of Cotton Leaf Response to Chemical Topping Based on iTRAQ Technique

HOU XiaoMeng1,2, LIU LianTao1,2, LI Meng4, SUN HongChun1,2, ZHANG YongJiang1,2, DU Huan2,3, LI CunDong2   

  1. 1College of Agronomy, Hebei Agricultural University, Baoding 071000, Hebei; 2Key Laboratory of Hebei Crop Growth Regulation, Baoding 071000, Hebei; 3College of Life Sciences, Hebei Agricultural University, Baoding 071000, Hebei; 4Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051
  • Received:2017-03-20 Online:2017-10-01 Published:2017-10-01

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Abstract: 【Objective】The objective of this study is to compare the effects of manual topping and chemical topping on physiological changes and proteins differentially expression of cotton. The results of this study would provide a theoretical basis for the mechanism of chemical topping. 【Method】Manual topping, chemical topping and no top pruning were practiced on cultivar GM863 which was planted in large area of the Yellow River basin in 2015-2016. Cotton was treated on July 20th simultaneously. Artificial spraying chemical topping agent and the dosage was 1.125 L·hm-2. The plant height and hormone contents of functional leaves were determined regularly after topping. The plant height was measured with the height from the cotyledon node to the growth point of the main stem by using ruler. The hormone contents of functional leaves, including IAA, GA3, ABA and ZR were measured by enzyme-linked immunosorbent assay. The differential proteomics analysis was carried out on the functional leaves of manual topping and chemical topping by using iTRAQ technique. 【Result】 Compared with the cotton of manual topping treatment, chemical topping treatment was significantly higher than manual topping treatment, which was 11.8% and 14.5% higher than others in the two-year trial, but was significantly lower than no top pruning, which was 6.0% and 6.5% lower than others, vertical growth was significantly inhibited after spraying. Different topping treatments greatly affected the GA3 content of cotton functional leaves, the change of GA3 content was a single peak curve, the contents of GA3 were no top pruning＞chemical topping＞manual topping, and reached significant difference. The GA3 contents of chemical topping and no top pruning were decreased at 30 d after topping and manual topping was decreased at 20 d. There was no significant difference among three treatments at 50 d after topping. The maximum IAA content in chemical topping treatment was significantly lower than that in the other two treatments at 40 d after topping in 2016, and there was no significant difference among the three treatments in 2015. The maximum ABA content in no top pruning treatment was significantly lower than that in the other two treatments at 40 d after topping. There was no significant difference among the three treatments of the contents of ZR. Compared with manual topping treatment, 69 differentially expressed proteins were detected by using iTRAQ in leaves of chemical topping treatment, 29 up-regulated and 40 down-regulated expressions, in which carbohydrate and energy metabolism related proteins were most down-regulated, and it was indicated that the growth of plants was decreased. The up-regulation of most GA regulated positive correlation proteins enhanced the GA effect. 【Conclusion】Chemical topping can effectively control the plant height of cotton, and which has a great effect on the GA content of functional leaves. The contents of GA in chemical topping treated leaves were significantly higher than that of manual topping treatment. Compared with manual topping treatment, the proteins related to plant growth and development in chemical topping treatment were most down-regulated expression, may be the plants reduced carbohydrate synthesis and energy metabolism, and activated GA effect by increasing GA content to control plant height.

Key words: cotton, chemical topping, hormone, iTRAQ technique, differential proteins

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