Scientia Agricultura Sinica ›› 2022, Vol. 55 ›› Issue (8): 1503-1517.doi: 10.3864/j.issn.0578-1752.2022.08.003


Characteristics and Cold Tolerance of Upland Cotton Genetic Standard Line TM-1

WANG JunJuan(),LU XuKe,WANG YanQin,WANG Shuai,YIN ZuJun,FU XiaoQiong,WANG DeLong,CHEN XiuGui,GUO LiXue,CHEN Chao,ZHAO LanJie,HAN YingChun,SUN LiangQing,HAN MingGe,ZHANG YueXin,FAN YaPeng,YE WuWei()   

  1. Institute of Cotton, Chinese Academy of Agricultural Sciences/State Key Laboratory of Cotton Biology/Key Laboratory for Cotton Genetic Improvement, Ministry of Agriculture and Rural Affairs, Anyang 455000, Henan
  • Received:2021-12-16 Accepted:2022-02-11 Online:2022-04-16 Published:2022-05-11
  • Contact: WuWei YE;


【Objective】We systematically investigated the major agronomic traits and cold tolerance of accession TM-1 at the bud and seedling stages. The relative expressions of cold tolerance-related genes were analyzed by the qRT-PCR method. The cold tolerance mechanism of TM-1 was further discussed, which provides the theoretical basis for the breeding utilization of TM-1.【Method】The major agronomic traits of TM-1 were manually investigated in the field using variety CRI35 as the control. The fiber quality was assessed by an international calibrated cotton standard (HVICC), and the insect resistance (Bt) was detected by kanamycin screening and molecular detection technologies. For the cold tolerance testing, two contrasting accessions, cold-resistant accession Yu 2067 and cold-sensitive variety Hengmian 3 were set as controls, respectively. The cold resistance of TM-1 at bud stage and cotyledon stage was identified, treated at 4℃ and then recovered under normal conditions for 7 days, and the relative cotyledon spreading rate and the cold injury levels of plants were investigated, and cold injury indexes and cold resistance indexes were calculated. The portable chlorophyll meter was used for in vivo testing the leaf relative chlorophyll content (represented by SPAD value). The expressions of cold tolerance-related genes in leaves were measured by qRT-PCR method. 【Result】 The leaves of TM-1 were large and dark green. The pre-frost seed cotton yield was 2 791.50 kg·hm-2, and the plant height was 94.60 cm. The growth period was about 135 days, and the yield, plant height, fruit branch number per plant, boll number per plant were higher than CRI35, while other agronomic traits were similar to CRI35. TM-1 had medium fiber quality. The test results of kanamycin and test paper showed that TM-1 did not contain the Bt like CRI35. Identification results of cold tolerance at bud stage showed that compared with the control treatment, the relative chlorophyll content and plant height of TM-1 decreased significantly. Low-temperature stress significantly inhibited hypocotyl elongation and chlorophyll synthesis in cotton leaves. Under low-temperature treatment, the taproots of TM-1 were damaged, but the lateral roots were more developed than those of the control. The cold tolerance level of TM-1 reached high cold resistance at the bud stage. Identification of cold tolerance at the cotyledon stage showed that the relative chlorophyll content and plant height of TM-1 decreased significantly compared with the control. The cold tolerance index of TM-1 at the cotyledon stage was 85.32%, which was significantly higher than Yu 2067, and the tolerance level of TM-1 reached cold resistance at the cotyledon stage. After the treatment of low-temperature stress for 24 h at the trefoil stage, nine genes were up-regulated in the TM-1 leaves, and their up-regulated expression folds were significantly higher than those of cold-sensitive accession. Dehydrin gene was up-regulated in TM-1 leaves, and the expression fold was similar to that in the leaves of Yu 2067, which was 4.69 times that in the leaves of Hengmian 3. The expression fold of the LEA3 gene in TM-1 leaves was significantly higher than that of Yu 2067 and Hengmian 3. 【Conclusion】 Accession TM-1 has stable agronomic characters and the medium fiber quality. It can be used as an ideal receptor for transferring exotic genes because without Bt. TM-1 can also be used as an important parent for cotton breeding and a gene source for cloning genes because of its good cold tolerance.

Key words: cotton, low temperature stress, TM-1, cold tolerance

Table 1

The origins and pedigrees of germplasm materials"

Origin or the breeding group
Parental combinations
TM-1 国家棉花种质中期库(安阳)
National Medium-term Genebank of Cotton Germplasm Resource in
China (Anyang)
The line D&PL-14
Yu 2067
Institute of Economic Crops, Henan Academy of Agricultural Sciences
CRI12/Yuzhi 177
Hengmian 3
Dry Farming Agricultural Research Institute, Hebei Academy of Agricultural and Forestry Sciences
Heng 9273/GK12
Institute of Cotton Research of Chinese Academy of Agricultural Sciences
Introduction of Bt and CpTI genes into CRI23
Institute of Cotton Research of Chinese Academy of Agricultural Sciences
Zhong 23021/(CRI12×Chuang 1704)

Table 2

Expression multiples in transcriptome and the primers of fluorescence quantitative real-time PCR of 11 chilling tolerance- related genes"

Gene ID
Gene functional annotations
log2Ratio (Treatment/Control)
Forward primer sequence (5′-3′)
Reverse primer sequence (5′-3′)
CotAD_50094 ZAT11 锌指蛋白ZAT11
Zinc finger protein ZAT11
CotAD_58358 COR47 脱水素COR47
Dehydrin COR47
CotAD_22633 ARG2 吲哚-3-乙酸诱导蛋白ARG2
Indole-3-acetic acid-induced protein ARG2
CotAD_74365 PBP1 钙信号转导的钙结合蛋白PBP1
Calcium-binding protein PBP1
CotAD_45286 CIPK6 CBL相互作用蛋白激酶
CBL-interacting serine/threonine-protein kinase 6
CotAD_09571 MPK3 丝裂原活化蛋白激酶3
Mitogen-activated protein kinase 3
CotAD_12521 EXPA8 细胞壁伸展蛋白A8
CotAD_76943 TRG-31 可能的水通道蛋白PIP 7a型
Probable aquaporin PIP-type 7a
CotAD_17081 WRKY46 可能的WRKY转录因子46
Probable WRKY transcription factor 46
CotAD_55002 NAC100 含NAC结构域的蛋白100 NAC
domain-containing protein 100
CotAD_56948 poxN1 过氧化物酶N1
Peroxidase N1

Table 3

Agronomic characters of tested materials"

Plant height
Number of fruit branches per plant
Number of bolls per plant
Single boll weight
Lint percentage
Unginned cotton yield (kg·hm-2)
TM-1 94.60Aa 12.80A 10.40A 6.55Aa 39.18Aa 2791.50A
中棉所35 CRI35 84.40Ab 9.10B 7.80B 5.65Ab 38.66Aa 2347.20B

Fig. 1

Transgenic characteristics test of tested materials A: Kanamycin screening; B: Molecular detection"

Fig. 2

Identification of cold tolerance of TM-1 at bud stage A: Three d of bud (CK) and bud after 4℃ low temperature treatment for 5 d; B: Schematic diagram of sowing; C and D: The seedlings of normal and low temperature treatments growing under normal conditions for 7 d"

Table 4

Identification of cold tolerance of TM-1 at bud stage"

3 d子叶平展率
The cotyledon spreading rate of 3 d (%)
7 d子叶平展率
The cotyledon spreading rate of 7 d (%)
7 d苗高
Plant height of 7 d
7 d相对叶绿素含量
The relative chlorophyll content of 7 d (SPAD value)
CK 95.56A 100.00Aa 10.05A 59.72A
4℃ 68.12B 94.74Aa 5.62B 39.63B

Fig. 3

Comparison of cold tolerance of three varieties (lines) at bud stage A: Buds germinating for 3 d (CK); B and F: The seedlings of CK treatments growing under normal conditions for 7 d; C: Buds of 3 d treated at 4℃ low temperature for 5 d; D and G: After 5 d of low temperature treatment, the seedlings recovered to grow for 7 d under normal conditions. E: Cotyledons of control and low temperature treatment after 7 d of recovery under normal conditions. The upper part of the red line was the control treatment, and the lower part was the low temperature treatment. TM-1, Yu 2067 and Hengmian 3 were shown from left to right in each drawing"

Table 5

Identification of cold tolerance of TM-1 in cotyledon stage"

The relative chlorophyll content (SPAD value)
Index of cold tolerance (%)
Level of cold tolerance
TM-1 CK 53.22Ab
4℃ 4 d 52.33Ab
CK恢复7 d Recovery for 7 d of CK 60.2Aa
4℃4 d恢复7 d Recovery for 7 d after 4℃4 d 53.91Ab 85.32Aa 抗冷Chilling resistant
Yu 2067
CK 52.61Ab
4℃4 d 51.76Ab
CK恢复7 d Recovery for 7 d of CK 60.8Aa
4℃4 d恢复7 d Recovery for 7 d after 4℃4 d 54.16Ab 76.55Ab 抗冷Chilling resistant
Hengmian 3
CK 51.12Ab
4℃4 d 41.14B
CK 恢复7 d Recovery for 7 d of CK 60.6Aa
4℃4 d恢复7 d Recovery for 7 d after 4℃4 d 41.48B 21.62B 冷敏感Chilling sensitive

Fig. 4

Identification of cold tolerance of TM-1 in cotyledon stage A: Control: The left side was the seedling before treatment, and the right side was the seedling after 7 d recovery; B: 4℃ low temperature treatment: The left side was the seedling before low temperature treatment, and the right side was the seedling after 7 d recovery; C: After 7 d of recovery, the leaves of control and 4℃ low temperature treatment were compared; The upper part was the control treatment, and the lower part was the 4℃ low temperature treatment. TM-1, Yu 2067 and Hengmian 3 was shown from left to right respectively in all figures"

Fig. 5

Morphological changes of TM-1 after 4℃ 24 h low temperature treatment CK: TM-1 control treatment; 4℃ 24 h: TM-1 was treated at 4℃ for 24 h"

Fig. 6

Expression analysis of cold tolerance related genes of TM-1, Yu 2067 and Hengmian 3 after low temperature treatment * : Significant differences (P<0.05); ** : Extremely significant differences (P<0.01)"

[1] KOHEL R J, LEWIS C F, RICHMOND T R. Texas marker-1: Description of a genetic standard for Gossypium hirsutum L.. Crop Science, 1970, 10(6): 670-671.
doi: 10.2135/cropsci1970.0011183X001000060019x
[2] GUO W Z, MA G J, ZHU Y C, YI C X, ZHANG T Z. Molecular tagging and mapping of quantitative trait loci for lint percentage and morphological marker genes in upland cotton. Journal of Integrative Plant Biology, 2006, 48(3): 320-326.
doi: 10.1111/j.1744-7909.2006.00174.x
[3] 王鹏, 丁业掌, 陆琼娴, 郭旺珍, 张天真. 陆地棉遗传标准系TM-1背景的海岛棉染色体片段置换系的培育. 科学通报, 2008, 53(9): 1065-1069.
WANG P, DING Y Z, LU Q X, GUO W Z, ZHANG T Z. Cultivation of Gossypium barbadense chromosome fragment replacement line with the background of upland cotton genetic standard line TM-1. Chinese Science Bulletin, 2008, 53(9): 1065-1069. (in Chinese)
[4] 付央, 苑冬冬, 胡文静, 蔡彩平, 郭旺珍. 陆地棉背景下海岛棉第18染色体片段置换系的培育及相关农艺性状QTL定位. 作物学报, 2013, 399(1): 21-28.
FU Y, YUAN D D, HU W J, CAI C P, GUO W Z. Development of Gossypium barbadense chromosome 18 segment substitution lines in the genetic standard line TM -1 of Gossypium hirsutum and mapping of QTLs related to agronomic traits. Acta Agronomica Sinica, 2013, 399(1): 21-28. (in Chinese)
[5] 李青, 鱼海鹏, 张子豪, 孙正文, 张艳, 张冬梅, 王省芬, 马峙英, 阎媛媛. 棉花真叶原生质体分离及瞬时表达体系的优化. 中国农业科学, 2021, 54(21): 4514-4524.
LI Q, YU H P, ZHANG Z H, SUN Z W, ZHANG Y, ZHANG D M, WANG S F, MA Z Y, YAN Y Y. Optimization of cotton mesophyll protoplast transient expression system. Scientia Agricultura Sinica, 2021, 54(21): 4514-4524. (in Chinese)
[6] LI F G, FAN G Y, LU C R, XIAO G H, ZOU C S, KOHEL R I, MA Z Y, SHANG H H, MA X F, WU J Y, LIANG X M, HUANG G, PERCY R G, LIU K, YANG W H, CHEN W B, DU X M, SHI C C, YUAN Y L, YE W W, LIU X, ZHANG X Y, LIU W Q, WEI H L, WEI S J, HUANG G D, ZHANG X L, ZHU S J, ZHANG H, SUN F M, WANG X F, LIANG J, WANG J H, HE Q, HUANG L H, WANG J, CUI J J, SONG G L, WANG K B, XU X, YU J Z, ZHU Y X, YU S X. Genome sequence of cultivated upland cotton (Gossypium hirsutum TM-1) provides insights into genome evolution. Nature Biotechnology, 2015, 33(5): 524-530.
doi: 10.1038/nbt.3208
[7] 赵晶, 李旭彤, 梁学忠, 王志城, 崔静, 陈斌, 吴立强, 王省芬, 张桂寅, 马峙英, 张艳. 陆地棉漆酶基因家族鉴定及在黄萎病菌胁迫下的表达分析. 作物学报, 2019(12): 1784-1795.
doi: 10.3724/SP.J.1006.2019.94053
ZHAO J, LI X T, LIANG X Z, WANG Z C, CUI J, CHEN B, WU L Q, WANG X F, ZHANG G Y, MA Z Y, ZHANG Y. Genome-wide identification of laccase gene family in update G. hirsutum L. genome and expression analysis under V. dahliae stress. Acta Agronomica Sinica, 2019(12): 1784-1795. (in Chinese)
doi: 10.3724/SP.J.1006.2019.94053
[8] YANG X M, LU X K, CHEN X G, WAGN D L, WANG J J, WANG S, GUO L X, CHEN C, WANG X G, WANG X L, YE W W. Genome-wide identification and expression analysis of DNA demethylase family in cotton. Journal of Cotton Research, 2019, 2(2): 142-150.
[9] DOU L L, LV L M, KANG Y Y, TIAN R J, HUANG D Q, LI J Y, LI S Y, LIU F P, CAOL Y, JIN Y H, LIU Y, LI H Z, WANGW B, PANG C Y, SHANG H H, ZOU C S, SONG G L, XIAO G H. Genome-wide identification and expression analysis of the GhIQD gene family in upland cotton (Gossypium hirsutum L.). Journal of Cotton Research, 2021, 4(1): 1-14.
doi: 10.1186/s42397-020-00077-x
[10] 王俊娟, 王帅, 陆许可, 阴祖军, 王德龙, 樊伟莉, 穆敏, 郭丽雪, 叶武威, 喻树迅. 棉花幼苗对低温胁迫的响应及抗冷机制初步研究. 棉花学报, 2017, 29(2): 34-43.
WANG J J, WANG S, LU X K, YIN Z J, WANG D L, FAN W L, MU M, GUO L X, YE W W, YU S X. The effect of low temperature stress on the growth of upland cotton seedings and a preliminary study of cold-resistance of mechanisms. Cotton Science, 2017, 29(2): 34-43. (in Chinese)
[11] 李鹏程, 郑苍松, 孙淼, 冯卫娜, 邵晶晶, 朱海勇, 李亚兵, 董合林. 不同形态氮肥对棉花15N回收率和产量的影响. 中国土壤与肥料, 2021(5): 53-57.
LI P C, ZHENG C S, SUN M, FENG W N, SHAO J J, ZHU H Y, LI Y B, DONG H L. Effects of different nitrogen forms on 15N recovery and yield of cotton. Soil and Fertilizer Sciences in China, 2021(5): 53-57. (in Chinese)
[12] 刘正德, 李运海, 罗云佳, 王红梅, 谭联望. 棉花新品种中棉所35的选育与应用技术. 中国棉花, 1999(11): 28-29.
LIU Z D, LI Y H, LUO Y J, WANG H M, TAN L W. Breeding and application technology of a new cotton variety CRI35. China Cotton, 1999(11): 28-29. (in Chinese)
[13] 罗晓丽, 张安红, 肖娟丽, 王志安, 陈晓英, 吴家和. 棉蚜腺苷三磷酸酶E亚基基因RNAi载体的构建及其抗蚜性分析. 棉花学报, 2018, 30(5): 353-362.
LUO X L, ZHANG A H, XIAO J L, WANG Z A, CHEN X Y, WU J H. Transgenic cotton expressing double-stranded RNAs of agATPase subunit E gene increases resistance to aphids. Cotton Science, 2018, 30(5): 353-362. (in Chinese)
[14] 王俊娟, 王德龙, 阴祖军, 王帅, 樊伟丽, 陆许可, 穆敏, 郭丽雪, 叶武威, 喻树迅. 陆地棉萌发至幼苗期抗冷性的鉴定. 中国农业科学, 2016, 49(17): 3332-3346.
WANG J J, WANG D L, YIN Z J, WANG S, FAN W L, LU X K, MU M, GUO L X, YE W W, YU S X. Identification of the chilling resistance from germination stage to seedling stage in upland cotton. Scientia Agricultura Sinica, 2016, 49(17): 3332-3346. (in Chinese)
[15] 王俊娟. 棉花抗冷性鉴定及相关基因的表达研究[D]. 北京: 中国农业科学院, 2016.
WANG J J. Identification of the chilling resistance of cotton and the expression of cold resistance related genes[D]. Beijing: Chinese Academy of Agricultural Sciences, 2016. (in Chinese)
[16] 段志坤, 秦晓惠, 朱晓红, 宋纯鹏. 解析植物冷信号转导途径: 植物如何感知低温. 植物学报, 2018, 53(2): 149-153.
doi: 10.11983/CBB18039
DUAN Z K, QIN X H, ZHU X H, SONG C P. Making sense of cold signaling: ICE is cold or not cold. Bulletin of Botany, 2018, 53(2): 149-153. (in Chinese)
doi: 10.11983/CBB18039
[17] ZHANG D J, GUO X Y, XU Y Y, LI H, MA L, YAO X F, WENG Y X, GUO Y, LIU C M, CHONG K. OsCIPK7point-mutation leads to conformation and kinase-activity change for sensing cold response. Journal of Integrative Plant Biology, 2019, 61(12): 1194-1200.
doi: 10.1111/jipb.12800
[18] KOBAYASHI M, HORIUCHI H, FUJITA K, TAKUHARA Y, SUZUKI S. Characterization of grape C-repeat-binding factor 2 and B-box-type zinc finger protein in transgenic Arabidopsis plants under stress conditions. Molecular Biology Reports, 2012, 39(8): 7933-7939.
doi: 10.1007/s11033-012-1638-4
[19] 倪晓详, 王晓荣, 陆军, 从青, 程龙军. 巨桉锌指结构蛋白基因EgrZFP7在低温胁迫中的功能研究. 农业生物技术学报, 2018, 26(8): 1288-1295.
NI X X, WANG X R, LU J, CONG Q, CHENG L J. Study on the function of zinc finger protein gent EgrZFP7 in cold stress response of Eucalyptus Grandis. Chinese Journal of Agricultural Biotechnology, 2018, 26(8): 1288-1295. (in Chinese)
[20] XU Y, HU W, LIU J H, SONG S, HOU X W, JIA C H, LI J Y, MIAO H X, WANG Z, TIE W W, XU B Y, JIN Z Q.An aquaporin gene MaPIP2-7 is involved in tolerance to drought, cold and salt stresses in transgenic banana(Musa acuminata L.). Plant Physiology and Biochemistry, 2020, 147: 66-76.
doi: 10.1016/j.plaphy.2019.12.011
[21] ZHANG Y, YU H J, YANG X Y, LI Q, LING J, WANG H, GU X F, HUANG S W, JIANG W J. CsWRKY46, a WRKY transcription factor from cucumber, confers cold resistance in transgenic-plant by regulating a set of cold-stress responsive genes in an ABA-dependent manner. Plant Physiology and Biochemistry, 2016, 108: 478-487.
doi: 10.1016/j.plaphy.2016.08.013
[22] CHEN G Y, LI Y H, WEI Z Z, GAN L, LIU J S, WANG Z. Dynamic profiles of DNA methylation and the interaction with histone acetylation during fiber cell initiation of Gossypium hirsutum. Journal of Cotton Research, 2022, 5(8): 1-14.
doi: 10.1186/s42397-021-00108-1
[23] 李俊兰, 崔淑芳, 金卫平, 黎鸿慧, 王广恩. 转Bt基因抗虫棉株卡那霉素抗性与抗虫性的关系研究. 河北农业科学, 2004(3): 112-113.
LI J L, CUI S F, JIN W P, LI H H, WANG G E. Study on the relation between kanamycine resistance and boll worm resistance of transgenic cotton. Journal of Hebei Agricultural Sciences, 2004(3): 112-113. (in Chinese)
[24] 吴雪霞, 查丁石, 邰翔. 低温胁迫对茄子幼苗生长、抗氧化酶活性和渗透调节物质的影响. 江苏农业学报, 2008(4): 471-475.
WU X X, ZHA D S, TAI X. Effects of low temperature stress on growth, activities of antioxidant enzymes and osmotic substance of eggplant seedlings. Jiangsu Journal of Agricultural Sciences, 2008(4): 471-475. (in Chinese)
[25] 尹晓斐. 低温胁迫对棉花生理特性的影响及关键酶基因表达分析[D]. 太原: 山西农业大学, 2013.
YIN X F. Effect of low temperature stress on physiological characteristics in cotton (Gossypium spp.) and expression analysis of key enzymes genes[D]. Taiyuan: Shanxi Agricultural University, 2013. (in Chinese)
[26] IVANOV A G, ROSSO D, SAVITCH L V, STACHULA P, ROSEMBERT M, OQUIST G, HURRY V, HVNER N P A. Implications of alternative electron sinks in increased resistance of PSII and PSI photochemistry to high light stress in cold-acclimated Arabidopsis thaliana. Photosynthesis Research, 2012, 113(1/3): 191-206.
doi: 10.1007/s11120-012-9769-y
[27] MU Q, LI X Y, LUO J H, PAN Q W, LI Y, GU T T. Characterization of expansin genes and their transcriptional regulation by histone modifications in strawberry. Planta, 2021, 254(2): 1-14.
doi: 10.1007/s00425-021-03652-x
[28] LI X X, ZHAO J, WALK T C, LIAO H. Characterization of soybean β-expansin genes and their expression responses to symbiosis, nutrient deficiency, and hormone treatment. Applied Microbiology & Biotechnology, 2014, 98(6): 2805-2817.
[29] LIU W M, XU L A, LIN H, CAO J S. Two expansin genes, AtEXPA4 and AtEXPB5, are redundantly required for pollen tube growth and AtEXPA4is involved in primary root elongation in Arabidopsis thaliana. Genes, 2021, 12(2): 249.
doi: 10.3390/genes12020249
[30] 刘栩铭, 李敏, 张曼, 霍红雁, 何智彪, 张继星, 王晓宇. 蓖麻3个PIP基因的克隆与冷胁迫下的表达分析. 农业生物技术学报, 2020, 28(10): 1788-1797.
LIU X M, LI M, ZHANG M, HUO H Y, HE Z B, ZHANG J X, WANG X Y. Cloning of 3 PIPs from Ricinus communis and expression analysis under cold stresses. Chinese Journal of Agricultural Biotechnology, 2020, 28(10): 1788-1797. (in Chinese)
[31] 鲁雨晴, 崔亚宁, 张原, 姚小敏, 李晓娟. 植物水通道蛋白PIPs亚细胞定位转运的研究进展. 电子显微学报, 2020, 39(6): 779-786.
LU Y Q, CUI Y N, ZHANG Y, YAO X M, LI X J. Advances in research on subcellular redistribution of plant plasma membrane aquaporin. Journal of Chinese Electron Microscopy Society, 2020, 39(6): 779-786. (in Chinese)
[32] POU A, JEANGUENIN L, MILHIET T, BATOKO H, XHAUMONT F, HACHEZ C.Salinity-mediated transcriptional and post-translational regulation of the Arabidopsis aquaporin PIP2;7. Plant Molecular Biology, 2016, 92(6): 1-14.
doi: 10.1007/s11103-016-0492-5
[33] 王俊娟, 穆敏, 王帅, 陆许可, 陈修贵, 王德龙, 樊伟丽, 阴祖军, 郭丽雪, 叶武威, 喻树迅. 棉花脱水素GhDHN1的克隆及其表达. 中国农业科学, 2016, 49(15): 2867-2878.
WANG J J, MU M, WANG S, LU X K, CHEN X G, WANG D L, FAN W L, YIN Z J, GUO L X, YE W W, YU S X.Molecular clone and expression of GhDHN1 gene in cotton (Gossypium hirsutum L.). Scientia Agricultura Sinica, 2016, 49(15): 2867-2878. (in Chinese)
[34] 王俊娟, 陆许可, 阴祖军, 王德龙, 王帅, 穆敏, 陈修贵, 郭丽雪, 樊伟丽, 陈超, 叶武威. 陆地棉GhLEA3基因的克隆及其响应低温胁迫表达分析. 棉花学报, 2019, 31(2): 89-100.
WANG J J, LU X K, YIN Z J, WANG D L, WANG S, MU M, CHEN X G, GUO L X, FAN W L, CHEN C, YE W W. Isolation and characterization of GhLEA3 gene from upland cotton and its expression in response to low temperature stress. Cotton Science, 2019, 31(2): 89-100. (in Chinese)
[35] GEORGE S, USHA B, PARIDA A. Isolation and characterization of an atypical LEA protein coding cDNA and its promoter from drought-tolerant plant Prosopis juliflora. Applied Biochemistry and Biotechnology, 2009, 157(2): 244-253.
doi: 10.1007/s12010-008-8398-6
[36] ATREYEE K, SUBHARTHI D, SOURAV B, YASUFUMI K, YURIKO K, KOYAMA H, MARKKANDAN G. GhSTOP1, a C2H2 type zinc finger transcription factor is essential for aluminum and proton stress tolerance and lateral root initiation in cotton. Plant Biology, 2019, 21(1): 35-44.
doi: 10.1111/plb.12895
[1] KAN JiaQiang, LIU Yu, ZHOU ZhiGuo, CHEN BingLin, ZHAO WenQing, HU Wei, HU ShaoHong, CHEN Yang, WANG YouHua. Effects of Squares and Bolls Abscission on Photosynthate Accumulation and Its Strength as an Auxiliary Source of Cotton Sympodial Leaves [J]. Scientia Agricultura Sinica, 2023, 56(9): 1658-1669.
[2] XING YuTong, TENG YongKang, WU TianFan, LIU YuanYuan, CHEN Yuan, CHEN Yuan, CHEN DeHua, ZHANG Xiang. Mepiquat Chloride Increases the Cry1Ac Protein Content Through Regulating Carbon and Amino Acid Metabolism of Bt Cotton Under High Temperature and Drought Stress [J]. Scientia Agricultura Sinica, 2023, 56(8): 1471-1483.
[3] WANG Ning, FENG KeYun, NAN HongYu, CONG AnQi, ZHANG TongHui. Effects of Combined Application of Organic Manure and Chemical Fertilizer Ratio on Water and Nitrogen Use Efficiency of Cotton Under Water Deficit [J]. Scientia Agricultura Sinica, 2023, 56(8): 1531-1546.
[4] JIA XiaoYun, WANG ShiJie, ZHU JiJie, ZHAO HongXia, LI Miao, WANG GuoYin. Construction of A High-Density Genetic Map and QTL Mapping for Yield Related Traits in Upland Cotton [J]. Scientia Agricultura Sinica, 2023, 56(4): 587-598.
[5] LIANG ChengZhen, ZANG YouYi, MENG ZhiGang, WANG Yuan, MUBASHIR Abbas, HE HaiYan, ZHOU Qi, WEI YunXiao, ZHANG Rui, GUO SanDui. Identification of Target Traits and Genetic Stability of Transgenic Cotton GGK2 [J]. Scientia Agricultura Sinica, 2023, 56(17): 3251-3260.
[6] WANG WanRu, CAO YueFen, SHENG Kuang, CHEN JinHong, ZHAO TianLun, ZHU ShuiJin. The Creation and Characteristics of Cotton Germplasm Lines Transgenic 1174AALdico-2+CTP Gene with Excellent Glyphosate Tolerance [J]. Scientia Agricultura Sinica, 2023, 56(17): 3261-3276.
[7] MA YanBin, LI HuanLi, WEN Jin, ZHOU XianTing, QIN Xin, WANG Xia, WANG XinSheng, LI YanE. Identification of Molecular Characterizations for Transgenic Cotton R1-3 Line of Glyphosate Tolerance [J]. Scientia Agricultura Sinica, 2023, 56(17): 3277-3284.
[8] DANG WenWen, LIU Bing, CHU Dong, LU YanHui. Dominated Species and the Predation Assessment of Natural Enemies on Thrips in Cotton Fields in Xinjiang [J]. Scientia Agricultura Sinica, 2023, 56(17): 3347-3357.
[9] LIU ZiGang, WEI JiaPing, CUI JunMei, WU ZeFeng, FANG Yan, DONG XiaoYun, ZHENG GuoQiang. Status, Existing Problems and Strategy Discussion on Northward Expansion of Winter Rapeseed in China [J]. Scientia Agricultura Sinica, 2023, 56(15): 2854-2862.
[10] LOU ShanWei, TIAN LiWen, LUO HongHai, DU MingWei, LIN Tao, YANG Tao, ZHANG PengZhong. Analysis on Key Production Techniques of Cotton with Good Quality and High Yield in Xinjiang [J]. Scientia Agricultura Sinica, 2023, 56(14): 2673-2685.
[11] ZHAO WeiSong, GUO QingGang, LI SheZeng, LU XiuYun, GOU JianJun, MA Ping. Effect of Broccoli Residues on Enzyme Activity of Cotton Rhizosphere Soil and Relationships Between Enzyme Activity and Carbon Metabolism Characteristics [J]. Scientia Agricultura Sinica, 2023, 56(11): 2092-2105.
[12] SONG Ci, GU FengXu, XING ZhenZhen, ZHANG JunMing, HE WenXue, WANG TianBo, WANG YuLu, CHEN JunYing. Physiological Changes and Integrity of ATP Synthase Subunits mRNA in Naturally Aged Cotton Seeds [J]. Scientia Agricultura Sinica, 2023, 56(10): 1827-1837.
[13] WANG CaiXiang,YUAN WenMin,LIU JuanJuan,XIE XiaoYu,MA Qi,JU JiSheng,CHEN Da,WANG Ning,FENG KeYun,SU JunJi. Comprehensive Evaluation and Breeding Evolution of Early Maturing Upland Cotton Varieties in the Northwest Inland of China [J]. Scientia Agricultura Sinica, 2023, 56(1): 1-16.
[14] DONG SangJie,JIANG XiaoChun,WANG LingYu,LIN Rui,QI ZhenYu,YU JingQuan,ZHOU YanHong. Effects of Supplemental Far-Red Light on Growth and Abiotic Stress Tolerance of Pepper Seedlings [J]. Scientia Agricultura Sinica, 2022, 55(6): 1189-1198.
[15] YIN YanYu, XING YuTong, WU TianFan, WANG LiYan, ZHAO ZiXu, HU TianRan, CHEN Yuan, CHEN Yuan, CHEN DeHua, ZHANG Xiang. Cry1Ac Protein Content Responses to Alternating High Temperature Regime and Drought and Its Physiological Mechanism in Bt Cotton [J]. Scientia Agricultura Sinica, 2022, 55(23): 4614-4625.
Full text



No Suggested Reading articles found!