玉米大斑病菌分生孢子形成的影响因素及GATA 转录因子家族的表达分析

doi:10.3864/j.issn.0578-1752.2017.07.006

Abstract

Abstract: 【Objective】 Northern leaf blight of corn is an important leaf disease and its agent is Setosphaeria turcica. The objectives of this study are to determine the main influencing factors in conidium yield and the expression characteristics of GATA transcription factor gene family at conidium formation stage in S. turcica. The research will lay a foundation for further illuminating the molecular mechanism of regulating growth, development and pathogenicity in S. turcica. 【Method】S. turcica wild-type strain 01-23 was used as test material in the study. The main factors which affecting conidium yield was analyzed as follows. The media were lactose casein agar, potato dextrose agar, corn stalk dextrose agar, corn stalk agar, corn leaf dextrose agar, corn leaf agar, corn niblet dextrose agar, corn niblet agar, corn starch dextrose agar, corn starch agar, czapek, minimal medium, and water agar. The carbons were lactose, glucose, sucrose, fructose, and maltose. Nitrogen sources were beef jelly, peptone, NH₄Cl, KNO₃, (NH₄)₂SO₄, NH₄NO₃, which were added in lactose casein agar medium respectively and the content was 5 g in per liter medium. The pHs were 4, 5, 6, 7, 8, and 9. The temperatures were 15, 20, 25, 30, and 35℃. The light intensities were 1 500, 3 000, 6 000, and 9 000 lx, respectively. quantitative real-time PCR (qRT-PCR) technology was employed to analyze the expression characteristics of GATA family members during conidium formation period. 【Result】 The optimum conditions for conidia yield in S. turcica based on single factor method were as follows. The most appropriate medium was maize leaf dextrose agar medium, pH was 8, 25℃, 6 000 lx light intensity (12 hours light and 12 hours dark). In addition, it was found that the addition of KNO₃in lactose casein agar medium significantly increased conidial yield. GATA2 significantly enhanced its expression level while other GATA family members, such as GATA1, GATA3, GATA4, GATA5, cut down their expression level at conidium formation stage compared with mycelium development stage. 【Conclusion】 The optimum culture condition of S. turcica conidium yield was determined based on single factor method in the study. According to the relative expression analysis of 5 genes in GATA family, it was speculated that GATA2 may be involved in the formation of conidia in S. turcica.

Key words: Setosphaeria turcica, conidium, sporulation condition, GATA gene family, gene expression

FENG ShengZe, LIU XingChen, WANG HaiXiang, ZHAO Jie, ZHAO LiQing, ZHENG YaNan, GONG XiaoDong, HAN JianMin, GU ShouQin, DONG JinGao. Influencing Factors of Conidiospore and Expression Analysis of GATA Transcription Factor Gene Family in Setosphaeria turcica[J].Scientia Agricultura Sinica, 2017, 50(7): 1234-1241.

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