Scientia Agricultura Sinica ›› 2017, Vol. 50 ›› Issue (4): 774-782.doi: 10.3864/j.issn.0578-1752.2017.04.017

• RESEARCH NOTES • Previous Articles    

Comparison of Healing Ability on Potato Tuber Cultivars ‘Qingshu No. 168’ and ‘Longshu No. 3’

JIANG Hong, BI Yang, LI ChangJian, WANG Yi, LI ShengE, LIU YaoNa, WANG Bin   

  1. College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070
  • Received:2016-08-01 Online:2017-02-16 Published:2017-02-16

Abstract: 【Objective】The objective of this study is to find out the differences of tuber healing ability of resistant and susceptible potato cultivars against dry rot (‘Qingshu No. 168’ and ‘Longshu No. 3’), and explore the reasons causing differences in the levels of lignin and suberin accumulation and phenylpropanoid metabolism.【Method】The resistant cultivar ‘Qingshu No. 168’ and susceptible cultivar ‘Longshu No. 3’ were used as materials. The damage of tubers was artificially simulated and heal was conducted at ambient temperature. The healing effect was assessed by determining the weight loss and disease index of wounded tubers that were inoculated with Fusarium sulphureum at every healing time point. Phloroglucinol-HCl staining and toluidin blue O-neutral red staining were used to observe lignin and suberin deposition, and the lignified cell layers and suberin fluorescent intensity were quantified. Moreover, the key enzyme and metabolites of phenylpropanoid metabolism pathway such as phenylalanine ammonia lyase activity, and the contents of lignin, total phenol and flavonoid were determined to compare the differences of healing ability between the two cultivars.【Result】The weight loss of ‘Qingshu No. 168’ was significantly lower than that of ‘Longshu No. 3’ during healing. The weight loss of ‘Qingshu No. 168’ and ‘Longshu No. 3’ was 0.93% and 1.96% after 14 days of healing, and the weight loss of ‘Qingshu No. 168’ was 52.3% lower than that of ‘Longshu No. 3’. The disease index of ‘Qingshu No. 168’ was also significantly lower than ‘Longshu No. 3’ after inoculated with F. sulphureum during healing. After 3 days of healing for inoculated tubers, the disease index of ‘Qingshu No. 168’ and ‘Longshu No. 3’ was 20.36 and 71.59, respectively, the former was 71.5% lower than the later. During healing, ‘Qingshu No. 168’ had higher accumulation rate of lignin and suberin than ‘Longshu No. 3’. After healing for 14 days, the thickness of lignified cell layers and total fluorescent intensity of suberin of ‘Qingshu No. 168’ were 47.4% and 60.6% higher than ‘Longshu No. 3’. In addition, ‘Qingshu No. 168’ showed more phenylpropanoid metabolism activity than ‘Longshu No. 3’. The activity of phenylalanine ammonialyase and the lignin content of ‘Qingshu No. 168’ were 77% and 65% higher than ‘Longshu No. 3’after healing for 14 days, respectively. The total phenolics contents of the two cultivars showed a significant difference at late stage of healing, ‘Qingshu No. 168’ was 76% higher than ‘Longshu No. 3’ after healing for 14 days. However, no significant difference was found in the flavonoids content between the two cultivars during healing.【Conclusion】The resistant cultivar ‘Qingshu No. 168’ showed stronger healing ability than susceptible cultivar ‘Longshu No. 3’. A stronger healing ability of resistant cultivar is related with its higher phenylpropanoid metabolism activity.

Key words: potato, cultivars, tuber, wound healing, phenylpropanoid metabolism

[1]    Dastmalchi K, Wang I, Stark R E. Potato wound-healing tissues: A rich source of natural antioxidant molecules with potential for food preservation. Food Chemistry, 2016, 210: 473-480.
[2]    Xue H L, Bi Y, Wei J M, Tang Y M, Zhao Y, Wang Y. Effect of cultivars, Fusarium strains and storage temperature on trichothecenes production in inoculated potato tubers. Food Chemistry, 2014, 151: 236-242.
[3]    Lulai E C. Skin-set, wound healing and related defects//Potato Biology and Biotechnology: Advances and Perspectives, 2007: 471-500.
[4]    Lulai E C, Suttle J C, Pederson S M. Regulatory involvement of abscisic acid in potato tuber wound-healing. Journal of Experimental Botany, 2008, 59(6): 1175-1186.
[5]    Lulai E C, Neubauer J D. Wound-induced suberization genes are differentially expressed, spatially and temporally, during closing layer and wound periderm formation. Postharvest Biology and Technology, 2014, 90: 24-33.
[6]    孙小娟, 李永才, 毕阳, 刘瑾, 尹艳. 西北地区马铃薯贮藏期病害调查分析. 中国马铃薯, 2009, 23(6): 364-365.
SUN X J, LI Y C, BI Y, LIU J, YIN Y. Investigation and analysis of disease on potato tubers during storage in Northwest China. Chinese Potato Journal, 2009, 23(6): 364-365. (in Chinese)
[7]    van Oirschot Q E A, Rees D, Aked J, Kihurani A. Sweetpotato cultivars differ in efficiency of wound healing. Postharvest Biology and Technology, 2006, 42(1): 65-74.
[8]    St AMAND P C, RANDLE W M. Ethylene production as a possible indicator of wound healing in roots of several sweet potato cultivars. Euphytica, 1991, 53(2): 97-102.
[9]    Dastmalchi K, Kallash L, Wang I, Phan V C, Huang W, Serra O, Stark R E. Defensive armor of potato tubers: nonpolar metabolite profiling, antioxidant assessment, and solid-state NMR compositional analysis of suberin-enriched wound-healing tissues. Journal of Agricultural and Food Chemistry, 2015, 63(30): 6810-6822.
[10]   Dastmalchi K, Cai Q, Zhou K, Huang W L, Serra O, Stark R E. Solving the jigsaw puzzle of wound-healing potato cultivars: metabolite profiling and antioxidant activity of polar extracts. Journal of Agricultural and Food Chemistry, 2014, 62(31): 7963-7975.
[11]   包改红, 毕阳, 李永才, 吴觉天, 寇宗红, 葛永红, 王毅, 王蒂. 不同愈伤时间对低温贮藏期间马铃薯块茎采后病害及品质的影响. 食品工业科技, 2013, 34(11): 330-334.
BAO G H, BI Y, LI Y C, WU J T, KOU Z H, GE Y H, WANG Y, WANG D. Effect of curing duration on incidence and quality of potato tuber during storage at low temperature. Science and Technology of Food Industry, 2013, 34(11): 330-334. (in Chinese)
[12]   Alba C M, De Forchetti S M, Tigier H A. Phenoloxidase of peach (Prunus persica) endocarp: Its relationship with peroxidases and lignification. Physiologia Plantarum, 2000, 109(4): 382-387.
[13]   Lulai E C, Morgan W C. Histochemical probing of potato periderm with neutral red: a sensitive cytofluorochrome for the hydrophobic domain of suberin. Biotechnic and Histochemistry, 1992, 67(4): 185-195.
[14]   Lulai E C, Corsini D L. Differential deposition of suberin phenolic and aliphatic domains and their roles in resistance to phenolic and aliphatic domains and their roles in resistance to infection during potato tuber (Solanum tuberosum L.) wound-healing. Physiological and Molecular Plant Pathology, 1998, 53(4): 209-222.
[15]   韩晶, 骞爱荣, 胡丽芳, 王哲, 于丹, 张蓉, 商澎. 基于免疫荧光图像的微管蛋白半定量分析. 第四军医大学学报, 2009, 30(24): 2901-2904.
HAN J, QIAN A R, HU L F, WANG Z, YU D, ZHANG R, SHANG P. Semi-quantitative analysis methods of tubulin based on the fluorescent images. Journal of the Fourth Military Medical University, 2009, 30(24): 2901-2904. (in Chinese)
[16]   吴觉天, 毕阳, 李永才, 包改红, 葛永红, 王蒂. 不同愈伤时间对常温贮藏期间马铃薯干腐病和品质的影响. 食品工业科技, 2013, 34(14): 332-334, 357.
WU J T, BI Y, LI Y C, BAO G H, GE Y H, WANG D. Wound curing in different time on dry rot and quality of potatoes during storage at room temperature. Science and Technology of Food Industry, 2013, 34(14): 332-334, 357. (in Chinese)
[17]   Yin Y, Li Y C, Bi Y, Cheng S J, Li Y, C, Yuan L, Wang Y, Wang D. Postharvest treatment with β-aminobutyric acid induces resistance against dry rot caused by Fusarium sulphureum in potato tuber. Agricultural Sciences in China, 2010, 9(9): 1372-1380.
[18]   Bernards M A. Demystifying suberin. Canadian Journal of Botany, 2002, 80(3): 227-240.
[19] Vishwanath S J, Delude C, Domergue F, Rowland    O. Suberin: biosynthesis, regulation, and polymer assembly of a protective extracellular barrier. Plant Cell Reports, 2015, 34(4): 573-586.
[20]   Soliday C L, Kolattukudy P E, Davis R W. Chemical and ultrastructural evidence that waxes associated with the suberin polymer constitute the major diffusion barrier to water vapor in potato tuber (Solanum tuberosum L.). Planta, 1979, 146(5): 607-614.
[21]   Bernards M A, Lewis N G. The macromolecular aromatic domain in suberized tissue: a changing paradigm. Phytochemistry, 1998, 47(6): 915-933.
[22]   Schreiber L, Franke R, Hartmann K. Wax and suberin development of native and wound periderm of potato (Solanum tuberosum L.) and its relation to peridermal transpiration. Planta, 2005, 220(4): 520-530.
[23]   Jarvinen R, Rauhala H, Holopainen U, Kallio H. Differences in suberin content and composition between two varieties of potatoes (Solanum tuberosum L.) and effect of post-harvest storage to the composition. LWT-Food Science and Technology,2011, 44(6): 1355-1361.
[24]   Morris S C, Forbes-Smith M R, Scrivenb F M. Determination of optimum conditions for suberization, wound periderm formation, cellular desiccation and pathogen resistance in wounded Solanum tuberosum tubers. Physiological and Molecular Plant Pathology, 1989, 35(2): 177-190.
[25]   Chaves I, Pinheiro C, Paiva1 J A, Planchon S, Sergeant K, Renaut J, Graça J A, Costa G, Coelho A V, Ricardo C P. Proteomic evaluation of wound-healing processes in potato (Solanum tuberosum L.) tuber tissue. Proteomics, 2009, 17(9): 4154-4175.
[26]   Franke1 R, Höfer R, Briesen I, Emsermann M, Efremova N, Yephremov A, Schreiber L. The DAISY gene from Arabidopsis encodes a fatty acid elongase condensing enzyme involved in the biosynthesis of aliphatic suberin in roots and the chalaza-micropyle region of seeds. The Plant Journal, 2009, 57(1): 80-95.
[27]   Yang W L, Bernards M A. Metabolite profiling of potato (Solanum tuberosum L.) tubers during wound-induced suberization. Metabolomics, 2007, 3(2): 147-159.
[28]   Yang W L, Bernards M A. Wound-induced metabolism in potato (Solanum tuberosum) tubers. Plant Signaling and Behavior, 2006, 1(2): 59-66.
[29]   Stadnik M J, Buchenauer H. Inhibition of phenylalanine ammonia-lyase suppresses the resistance induced by benzothiadiazole in wheat to Blumeria graminis f. sp. tritici. Physiological and Molecular Plant Pathology, 2000, 57(1): 25-34.
[30]   Borg-Olivier O, Monties B. Lignin, suberin, phenolic acids and tyramine in the suberized, wound-induced potato periderm. Phytochemistry, 1993, 32(3): 601-606.
[31]   Kolattukudy P E. Biopolyester membranes of plants: cutin and suberin. Science, 1980, 208(4447): 990-1000.
[32]   Hammerschmidt R. Rapid deposition of lignin in potato tuber tissue as a response to fungi non-pathogenic on potato. Physiological Plant Pathology, 1984, 24(1): 33-42.
[33]   Bernards M A, Susag L M, Bedgar D L, Anterola A M, Lewis N G. Induced phenylpropanoid metabolism during suberization and lignification: a comparative analysis. Journal of Plant Physiology, 2000, 157(6): 601-607.
[34]   Bernards M A, Lopez M L, Zajicek J, Lewis N G. Hydroxycinnamic acid-derived polymers constitute the polyaromatic domain of suberin. The Journal of Biological Chemistry, 1995, 270(13): 7382-7386.
[35]   Valentines M C, Vilaplana R, Torres R, Usall J, Larrigaudiere C. Specific roles of enzymatic browning and lignification in apple disease resistance. Postharvest Biology and Technology, 2005, 36(3): 227-234.
[36]   Thomas R, Fang X, Ranathunge K, Anderson T R, Peterson C A, Bernards M A. Soybean root suberin: anatomical distribution, chemical composition, and relationship to partial resistance to Phytophthora sojae. Plant Physiology, 2007, 144(1): 299-311.
[37]   Shao X F, Kang T, Tu S C, Su J, Zhao Y. Effects of heat treatment on wound healing in Gala and Red Fuji apple fruits. Journal of Agricultural and Food Chemistry, 2010, 58(7): 4303-4309.
[38]   Ghanekar A S, Padwal-Desai S R, Nadkarni G B. The involvement of phenolics and phytoalexins in resistance of potato to soft rot. Potato Research, 1984, 27(2): 189-199.
[39]   Back K. Hydroxycinnamic acid amides and their possible utilization for enhancing agronomic traits. The Plant Pathology Journal, 2001, 17(3): 123-127.
[40]   Arrieta-Baez D, Stark R E. Modeling suberization with peroxidase-catalyzed polymerization of hydroxycinnamic acids: cross-coupling and dimerization reactions. Phytochemistry, 2006, 67(7): 743-753.
[41] Razem F A, Bernards M A. Hydrogen peroxide is required for poly (phenolic) domain formation during wound-induced suberization. Journal of Agricultural and Food Chemistry, 2002, 50(5): 1009-1015.
[42]   Mahgouba H A M, Eisab G S A, Youssefc M A H. Molecular, biochemical and anatomical analysis of some potato (Solanum tuberosum L.) cultivars growing in Egypt. Journal of Genetic Engineering and Biotechnology, 2015, 13(1): 39-49.
[1] PENG Xue,GAO YueXia,ZHANG LinXuan,GAO ZhiQiang,REN YaMei. Effects of High-Energy Electron Beam Irradiation on Potato Storage Quality and Bud Eye Cell Ultrastructure [J]. Scientia Agricultura Sinica, 2022, 55(7): 1423-1432.
[2] CUI Peng,ZHAO YiRen,YAO ZhiPeng,PANG LinJiang,LU GuoQuan. Starch Physicochemical Properties and Expression Levels of Anabolism Key Genes in Sweetpotato Under Low Temperature [J]. Scientia Agricultura Sinica, 2022, 55(19): 3831-3840.
[3] XiaoChuan LI,ChaoHai WANG,Ping ZHOU,Wei MA,Rui WU,ZhiHao SONG,Yan MEI. Deciphering of the Genetic Diversity After Field Late Blight Resistance Evaluation of Potato Breeds [J]. Scientia Agricultura Sinica, 2022, 55(18): 3484-3500.
[4] BaoHua CHU,FuGuo CAO,NingNing BIAN,Qian QIAN,ZhongXing LI,XueWei LI,ZeYuan LIU,FengWang MA,QingMei GUAN. Resistant Evaluation of 84 Apple Cultivars to Alternaria alternata f. sp. mali and Genome-Wide Association Analysis [J]. Scientia Agricultura Sinica, 2022, 55(18): 3613-3628.
[5] ZHANG XiaoPing,SA ShiJuan,WU HanYu,QIAO LiYuan,ZHENG Rui,YAO XinLing. Leaf Stomatal Close and Opening Orchestrate Rhythmically with Cell Wall Pectin Biosynthesis and Degradation [J]. Scientia Agricultura Sinica, 2022, 55(17): 3278-3288.
[6] LI WenLi, YUAN JianLong, DUAN HuiMin, JIANG TongHui, LIU LingLing, ZHANG Feng. Comprehensive Evaluation of Potato Tuber Texture [J]. Scientia Agricultura Sinica, 2022, 55(12): 2278-2293.
[7] FAN WenJing,LIU Ming,ZHAO Peng,ZHANG QiangQiang,WU DeXiang,GUO PengYu,ZHU XiaoYa,JIN Rong,ZHANG AiJun,TANG ZhongHou. Screening of Sweetpotato Varieties Tolerant to Low Nitrogen at Seedling Stage and Evaluation of Different Nitrogen Efficiencies [J]. Scientia Agricultura Sinica, 2022, 55(10): 1891-1902.
[8] YuXin LIANG,JianXiang WU,XiaoYu LI,ChunYu ZHANG,JiChao HOU,XuePing ZHOU,YongZhi WANG. Mapping of Epitopes and Establishment of Rapid DAS-ELISA for Potato Virus Y Coat Protein [J]. Scientia Agricultura Sinica, 2021, 54(6): 1154-1162.
[9] JianZhao TANG,Jing WANG,DengPan XIAO,XueBiao PAN. Research Progress and Development Prospect of Potato Growth Model [J]. Scientia Agricultura Sinica, 2021, 54(5): 921-932.
[10] LI KaiFeng,YIN YuHe,WANG Qiong,LIN TuanRong,GUO HuaChun. Correlation Analysis of Volatile Flavor Components and Metabolites Among Potato Varieties [J]. Scientia Agricultura Sinica, 2021, 54(4): 792-803.
[11] WANG Xin,LI Qiang,CAO QingHe,MA DaiFu. Current Status and Future Prospective of Sweetpotato Production and Seed Industry in China [J]. Scientia Agricultura Sinica, 2021, 54(3): 483-492.
[12] ZHANG MengDi,YAN JunJie,GAO YuLin. The Adaptive Analysis of Phthorimaea operculella to Different Potato Tuber Varieties [J]. Scientia Agricultura Sinica, 2021, 54(3): 536-546.
[13] LI Xiang,ZHANG XiaoJiao,XIAO Chun,DONG WenXia. Electroantennogram Responses of Phthorimaea operculella of Different Sexes and Mating States to Potato Volatiles [J]. Scientia Agricultura Sinica, 2021, 54(3): 547-555.
[14] CHEN Yang,ZHAO HongYi,YAN JunJie,HUANG Jian,GAO YuLin. Chemical Synthesis View on Sex Pheromones of Potato Tuberworm (Phthorimaea operculella) [J]. Scientia Agricultura Sinica, 2021, 54(3): 556-572.
[15] XIONG Yan,HAN Rui,HU ChunHua,WANG Jing,XIAO Chun. Influences of Chemical and Physical Stimuli on Oviposition Behavior of Phthorimaea operculella [J]. Scientia Agricultura Sinica, 2021, 54(3): 573-582.
Full text



No Suggested Reading articles found!