Scientia Agricultura Sinica ›› 2021, Vol. 54 ›› Issue (3): 547-555.doi: 10.3864/j.issn.0578-1752.2021.03.009

• SPECIAL FOCUS: GREEN CONTROL FOR POTATO TUBERWORM (PHTHORIMAEA OPERCULELLA) • Previous Articles     Next Articles

Electroantennogram Responses of Phthorimaea operculella of Different Sexes and Mating States to Potato Volatiles

LI Xiang(),ZHANG XiaoJiao,XIAO Chun,DONG WenXia()   

  1. College of Plant Protection/State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201
  • Received:2020-06-30 Accepted:2020-07-28 Online:2021-02-01 Published:2021-02-16
  • Contact: WenXia DONG E-mail:lixiang0217@126.com;dongwenxia@163.com

Abstract:

【Objective】The objective of this study is find out the difference of electrophysiological responses of potato tuberworm (Phthorimaea operculella) with different sexes and mating states to potato volatiles, and to provide a reference for the chemical ecological control of P. operculella.【Method】Electroantennogram (EAG) responses of virgin female, mated female and unmated male P. operculella to 16 volatile compounds (including 6 aliphatic compounds viz. 1-octen-3-ol,1-butanol, heptanal, octanal, nonanal and decanal, 4 aromatic compounds viz. phenethyl alcohol, phenylacetaldehyde, acetophenone and methyl salicylate, and 6 terpenoids viz. α-pinene, p-cymene, (E)-β-ocimene, 1,8-cineole, (E)-β-farnesene and methyl jasmonate) released from potato tubers and plants were recorded, and the dose-responses to 6 compounds (including 1-octen-3-ol, heptanal, nonanal, acetophenone, methyl salicylate and (E)-β-ocimene) were also conducted.【Result】All the tested compounds could elicit EAG responses of P. operculella. Among them, the aliphatic alcohols elicited significantly greater EAG values in the virgin females and the unmated males, and aliphatic aldehydes elicited significantly greater EAG values in the mated females. However, the terpenoids elicited lower EAG values in all the tested P. operculella. At the dose of 1 000 μg, the same compound elicited varied antennal responses from the P. operculella with different sexes and mating states. Octanal, methyl salicylate and (E)-β-ocimene elicited stronger responses in virgin females than those in the mated females and the unmated males, while heptanal, nonanal and α-pinene elicited stronger responses in the mated females than those in the others. The compounds 1-octene-3-ol, phenethyl alcohol and phenylacetaldehyde elicited stronger responses in the males than those in the females. At the tested dose series (1, 10, 100 and 1 000 μg), EAG responses of P. operculella at 3 physiological states to 6 individual compounds increased with the increase of stimulation dose, and were far from saturation thresholds. For the same compound at different doses, EAG responses of the mated females varied significantly.【Conclusion】The virgin females and the unmated males of P. operculella are more sensitive to aliphatic alcohols, and the mated females are more sensitive to aliphatic aldehydes. However, both the females and males are less sensitive to terpenoid compounds. Among the responses to the individual compound, the mated females are more sensitive to heptanal, nonanal and α-pinene than the virgin females and the unmated males, so are their responses to the same compound at different doses.

Key words: potato tuberworm (Phthorimaea operculella), potato volatile, electroantennogram response

Fig. 1

The EAG responses of virgin female, mated female and unmated male P. operculella to 16 volatile compounds The different lowercases showed that there existed significant differences among the relative EAG values of P. operculella at the same physiological state to different compounds by Tukey’s test (P<0.05)"

Fig. 2

The comparison of EAG responses of virgin female, mated female and unmated male P. operculella to the same volatile compound The different lowercases showed that there existed significant differences among the relative EAG values of virgin females, mated females and unmated males to the same compound by Tukey’s test (P<0.05)"

Fig. 3

EAG responses of virgin female, mated female and unmated male P. operculella to 6 volatile compounds at different doses The different lowercases showed that there existed significant differences among the relative EAG values of P. operculella at the same physiological state to the same compound at different doses by Tukey’s test (P<0.05)"

[1] RONDON S I. The potato tuber worm: A literature review of its biology, ecology and control. American Journal of Potato Research, 2010,87(2):149-166.
[2] RONDON S I. Decoding Phthorimaea operculella (Lepidoptera: Gelechiidae) in the new age of change. Journal of Integrative Agriculture, 2020,19(2):316-324.
[3] GAO Y. Potato tuberworm: A threat for China potatoes. Entomology, Ornithology and Herpetology: Current Research, 2018,7(2):1000e132.
[4] 高玉林, 徐进, 刘宁, 周倩, 丁新华, 詹家绥, 成新跃, 黄剑, 鲁宇文, 杨宇红. 我国马铃薯病虫害发生现状与防控策略. 植物保护, 2019,45(5):106-111.
GAO Y L, XU J, LIU N, ZHOU Q, DING X H, ZHAN J S, CHENG X Y, HUANG J, LU Y W, YANG Y H. Current status and management strategies for potato insect pests and diseases in China. Plant Protection, 2019,45(5):106-111. (in Chinese)
[5] ARAB A, TRIGO J R, LOURENCÃO A L, PEIXOTO A M, RAMOS F, BENTO J M S . Differential attractiveness of potato tuber volatiles to Phthorimaea operculella (Gelechiidae) and the predator Orius insidiosus (Anthocoridae). Journal of Chemical Ecology, 2007,33(10):1845-1855.
pmid: 17885793
[6] SHARABY A, ABDEL-RAHMAN H, MOAWAD S. Biological effects of some natural and chemical compounds on the potato tuber moth, Phthorimaea operculella Zeller (Lepidoptera: Gelechiidae). Saudi Journal of Biological Sciences, 2009,16(1):1-9.
doi: 10.1016/j.sjbs.2009.07.001 pmid: 23961036
[7] 马艳粉, 胥勇, 肖春. 10种寄主植物挥发物对马铃薯块茎蛾产卵的引诱作用. 中国生物防治学报, 2012,28(3):448-452.
MA Y F, XU Y, XIAO C. Oviposition attraction effect of ten host-plant volatiles on potato tuber moth, Phthorimaea operculella. Chinese Journal of Biological Control, 2012,28(3):448-452. (in Chinese)
[8] 马艳粉, 张晓梅, 胥勇, 肖春. 滇杨挥发物成分对马铃薯块茎蛾产卵选择的影响. 植物保护, 2016,42(2):99-103.
MA Y F, ZHANG X M, XU Y, XIAO C. Effects of volatiles from Populus yunanensis on oviposition preferece of potato tuber moth, Phthorimaea operculella. Plant Protection, 2016,42(2):99-103. (in Chinese)
[9] 刘燕, 谢冬生, 熊焰, 王春娅, 任智强, 肖春. 庚醛与桉叶油醇组合对马铃薯块茎蛾产卵选择的影响. 植物保护, 2016,42(3):99-103.
LIU Y, XIE D S, XIONG Y, WANG C Y, REN Z Q, XIAO C. Effects of combination of cineole with heptanal on oviposition choices of the potato tuber moth, Phthorimaea operculella. Plant Protection, 2016,42(3):99-103. (in Chinese)
[10] DEKEBO A, ARYAL S, JUNG C. Olfactory responses of adult potato tuber moth, Phthorimaea operculella (Zeller) measured by attraction relative to the tomato leaf volatiles. Journal of Asia-Pacific Entomology, 2019,22(2):611-618.
[11] LI X, ZHANG X G, XIAO C, GAO Y L, DONG W X. Behavioral responses of potato tuber moth (Phthorimaea operculella) to tobacco plant volatiles. Journal of Integrative Agriculture, 2020,19(2):325-332.
[12] 张小娇, 钱诚, 杨六三, 刘洪翠, 李成云, 董文霞. 不同生理状态的番石榴实蝇对寄主气味的行为反应. 应用昆虫学报, 2020,57(1):166-172.
ZHANG X J, QIAN C, YANG L S, LIU H C, LI C Y, DONG W X. Behavioral responses of sexually immature, mature virgin, and mated, guava fruit flies to host plant odor. Chinese Journal of Applied Entomology, 2020,57(1):166-172. (in Chinese)
[13] MECHABER W L, CAPALDO C T, HILDEBRAND J G. Behavioral responses of adult tobacco hornworms, Manduca sexta, to host plant volatiles change with age and mating status. Journal of Insect Science, 2002,2(1):5.
[14] YAN F, BENGTSSON M, WITZGALL P. Behavioral response of female codling moths, Cydia pomonella, to apple volatiles. Journal of Chemical Ecology, 1999,25(6):1343-1351.
[15] LEMMEN-LECHELT J K, WIST T J, EVENDEN M L. State-dependent plasticity in response to host-plant volatiles in a long-lived moth, Caloptilia fraxinella (Lepidoptera: Gracillariidae). Journal of Chemical Ecology, 2018,44(3):276-287.
doi: 10.1007/s10886-018-0927-3 pmid: 29396790
[16] TANG R, SU M W, ZHANG Z N. Electroantennogram responses of an invasive species fall webworm (Hyphantria cunea) to host volatile compounds. Chinese Science Bulletin, 2012,57(35):4560-4568.
[17] ZHANG Z, BIAN L, SUN X, LUO Z, XIN Z, LUO F, CHEN Z. Electrophysiological and behavioural responses of the tea geometrid Ectropis obliqua (Lepidoptera: Geometridae) to volatiles from a non-host plant, rosemary, Rosmarinus officinalis (Lamiaceae). Pest Management Science, 2015,71(1):96-104.
pmid: 24616107
[18] SHU S, GRANT G G, LANGEVIN D, LOMBARDO D A, MACDONALD L. Oviposition and electroantennogram responses of Dioryctria abietivorella (Lepidoptera: Pyralidae) elicited by monoterpenes and enantiomers from eastern with pine. Journal of Chemical Ecology, 1997,23(1):35-50.
[19] MARTEL V, ANDERSON P, HANSSON B S, SCHLYTER F. Peripheral modulation of olfaction by physiological state in the Egyptian leaf worm Spodoptera littoralis (Lepidoptera: Noctuidae). Journal of Insect Physiology, 2009,55(9):793-797.
pmid: 19414013
[20] SAVEER A M, KROMANN S H, BIRGERSSON G, BENGTSSON M, LINDBLOM T, BALKENIUS A, HANSSON B S, WITZGALL P, BECHER P G, IGNELL R. Floral to green: Mating switches moth olfactory coding and preference. Proceedings of Royal Society B: Biological Sciences, 2012,279(1737):2314-2322.
[21] DAS P D, RAINA R, PRASAD A R, SEN A. Electroantennogram responses of the potato tuber moth, Phthorimaea operculella (Lepidoptera; Gelichiidae) to plant volatiles. Journal of Biosciences, 2007,32(2):339-349.
pmid: 17435325
[22] 王国红, 刘勇, 戈峰, 吴开拓. 粉蝶盘绒茧蜂中国和荷兰种群学习行为及EAG反应的比较. 生态学报, 2012,32(2):351-360.
WANG G H, LIU Y, GE F, WU K T. Comparative study on learning behavior and electroantennogram responses in two geographic races of Cotesia glomerata. Acta Ecologica Sinica, 2012,32(2):351-360. (in Chinese)
[23] BOLTER C J, DICKE M, VAN LOON J J A, VISSER J H, POSTHUMUS M A . Attraction of Colorado potato beetle to herbivore-damaged plants during herbivory and after its termination. Journal of Chemical Ecology, 1997,23(4):1003-1023.
[24] AGELOPOULOS N G, CHAMBERLAIN K, PICKETT J A. Factors affecting volatile emissions of intact potato plants, Solanum tuberosum: Variability of quantities and stability of ratios. Journal of Chemical Ecology, 2000,26(2):497-511.
[25] KARLSSON M F, BIRGERSSON G, PRADO A M C, BOSA F, BENGTSSON M, WITZGALL P. Plant odor analysis of potato: Response of Guatemalan moth to above- and background potato volatiles. Journal of Agricultural and Food Chemistry, 2009,57(13):5903-5909.
doi: 10.1021/jf803730h pmid: 19496533
[26] BRUCE T J A, PICKETT J A. Perception of plant volatile blends by herbivorous insects—Finding the right mix. Phytochemistry, 2011,72(13):1605-1611.
pmid: 21596403
[27] SOLE J, SANS A, RIBA M, GUERRERO A. Behavioural and electrophysiological responses of the European corn borer Ostrinia nubilalis to host-plant volatiles and related chemicals. Physiological Entomology, 2010,35:354-363.
[28] ANESBO L, CORACINI M D A, BENGTSSON M, LIBLIKAS I, RAMIREZ M, BORG-KARLSON A K, TASIN M, WITZGALL P . Antennal and behavioural response of codling moth Cydia pomonella to plant volatiles. Journal of Applied Entomology, 2004,128(7):488-493.
[29] SCHMIDT-BUSSER D, VON ARX M, CONNETABLE S, GUERIN P M. Identification of host-plant chemical stimuli for the European grape berry moth Eupoecilia ambiguella. Physiological Entomology, 2011,36:101-110.
[30] MA Y F, XIAO C. Push-pull effects of three plant secondary metabolites on oviposition of the potato tuber moth, Phthorimaea operculella. Journal of Insect Science, 2013,13(1):128.
[31] ANFORA G, VITAGLIANO S, LARSSON M C, WITZGALL P, TASIN M, GERMINARA G S, DE CRISTOFARO A. Disruption of Phthorimaea operculella (Lepidoptera: Gelechiidae) oviposition by the application of host plant volatiles. Pest Management Science, 2014,70:628-635.
pmid: 23794160
[32] 张治科, 吴圣勇, 雷仲仁. 西花蓟马气味结合蛋白的cDNA克隆、序列分析及时空表达. 中国农业科学, 2016,49(6):1106-1116.
ZHANG Z K, WU S Y, LEI Z R. Cloning, sequence analysis and expression profile of an odorant binding protein gene in western flower thrips (Frankliniella occidentalis). Scientia Agricultura Sinica, 2016,49(6):1106-1116. (in Chinese)
[33] 陈丽慧, 李梅梅, 陈秀琳, 仵均祥, 许向利. 梨小食心虫普通气味受体基因GmolOR20的克隆及表达分析. 昆虫学报, 2019,62(4):418-427.
CHEN L H, LI M M, CHEN X L, WU J X, XU X L. Cloning and expression profiling of general odorant receptor gene GmolOR20 in the oriental fruit moth, Grapholita molesta (Lepidoptera: Tortricidae). Acta Entomologica Sinica, 2019,62(4):418-427. (in Chinese)
[1] ZHANG MengDi,YAN JunJie,GAO YuLin. The Adaptive Analysis of Phthorimaea operculella to Different Potato Tuber Varieties [J]. Scientia Agricultura Sinica, 2021, 54(3): 536-546.
[2] CHEN Yang,ZHAO HongYi,YAN JunJie,HUANG Jian,GAO YuLin. Chemical Synthesis View on Sex Pheromones of Potato Tuberworm (Phthorimaea operculella) [J]. Scientia Agricultura Sinica, 2021, 54(3): 556-572.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!