Scientia Agricultura Sinica ›› 2016, Vol. 49 ›› Issue (23): 4480-4487.doi: 10.3864/j.issn.0578-1752.2016.23.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

QTL Analysis of Test Weight Dynamic Change in Maize

XU Meng-meng1, QIN Yong-tian2, CHEN Yong-qiang1, ZHANG Zhan-hui1, TANG Ji-hua1, FU Zhi-yuan1   

  1. 1College of Agronomy, Henan Agricultural University, Zhengzhou 450002
    2Hebi Academy of Agricultural Sciences, Hebi 456284, Henan
  • Received:2016-05-18 Online:2016-12-01 Published:2016-12-01

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Abstract: 【Objective】Identifying the QTLs controlling test weight dynamic change during maize grain filling will benefit for gene cloning of the related key QTLs. 【Method】In 2009 and 2010, a set of RIL population with 166 inbred lines derived from hybrid Nongda108 (Xu178 crossed with Huang C) was planted in Zhengzhou and Anyang with randomized block design. The ear samples of recombinant inbred lines with the same silking date were hand-harvested performed by time-course harvesting on 15, 22, 29, 36, 43, and 50 days after pollination for test weight evaluation. Phenotypic analysis was performed using SPSS18.0 software. The 822 pairs of SSR covering the whole maize genome were used to detect the polymorphisms between the two parents. And, 216 SSR markers were selected for linkage map construction. With the linkage map, QTLs responsible for test weight were evaluated by composite interval mapping program in the WinCartQTL 2.5 software. 【Result】Test weight of Nongda108 and its two parents tended to be a slow-fast-slow pattern along with the grain filling process. Genetic factor showed predominant factor on test weight, while environmental factors mainly did their effects during the early and the late stages rather than the peak stage. Between two years, the test weight of DAP22-DAP43 showed a significant difference, but no significant difference at the last stage. The test weight of hybrid Nongda108 was among the two parents exhibiting typical additive effects within different developmental periods. Via time-related QTL analysis, 31 QTLs were detected for test weight of six sampling stages under four environments. These QTLs were located on all the 10 chromosomes of maize genome with exception of chromosomes 4 and 10. And, the 2, 4, 5, 3, 4, 5, 3, and 3 QTLs were distributed on chromosomes 1, 2, 3, 5, 6, 7, 8 and 9, respectively. Among them, additive effect of 13 QTLs was contributed by Huang C (+), while additive effect of another 18 QTLs was contributed by Xu178 (-). The contribution of single QTL varied from 5.9% to 29.7%. qTW2c was detected at DAP22 and DAP36 with a contribution of 11.5% and 14.7% to test weight in Anyang in 2010. qTW3c was detected on DAP29 and DAP36 with a contribution of 22.2% and 14.7% to test weight in Zhengzhou in 2009. 【Conclusion】Two conserved QTLs, qTW2c and qTW3c, were repeatedly detected at two sampling stages with more than 10% contribution to test weight. These two major QTLs are very important for cloning gene controlling test weight dynamic change.

Key words: maize, grain filling, test weight, QTL

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