玉米秸秆低温高效降解复合菌系GF-20的菌种组成及降解稳定性

doi:10.3864/j.issn.0578-1752.2016.03.004

Abstract

Abstract: 【Objective】 In order to improve the culturing methodology of corn stalk decomposing microbes and promote its utilization, the present study evaluated the impact of different culturing conditions on the community composition of a composite microbial system GF-20 and on its decomposing activity of corn stalk.【Method】 Composite microbial system GF-20 was continuously sub-cultured to the 45th generation under 10℃ and to 15th generation under different temperatures and pH conditions to obtain different microbial communities (F, T, P). Then the dynamics of fermenting pH, corn stalk decomposing ratio and cellulose enzyme activities were determined, so as to estimate the corn stalk decomposing activity of the composite microbial system. Furthermore, the community composition stability was analyzed by PCR-DGGE technique combined with a principal component analysis. 【Result】 The results showed that the pH value tended to be neutral over the fermentation time, and the straw degradation rate ranged in 27.59%-32.53%, in which no difference was shown among the successive 40-generation sub-cultured communities except F40 and F5, neither among the sub-cultured communities under temperature of 4-30℃ and under pH of 6.0-9.0. Cellulose enzyme activity of high generation was higher than that of low generation, and the enzyme production of the microbial community could be promoted under temperature of 4-10℃ and pH of 6.0-9.0, cellulase activity ranged in 1.34-1.84 IU·mL^-1. Cellulase activity of composite microbes showed good stability under lower temperature and in a wide pH range, enzymatic reaction temperature within 15-30℃ and pH within 4.0-9.0 could keep more than 80% of enzyme activity. Furthermore, DGGE bands of F5-F45, T4-T30 and P6-P9 showed no significant differences indicated that the strains composition maintained good stability. However, under acidic (pH=4, 5) or alkaline (pH=10) subculture conditions, the corn stalk degradation rate and cellulase activity significantly decreased, and community composition also changed remarkably, thereby affected the property and functional stability. According to PCR-DGGE profiles, a total of 18 strains were detected, the key strains of which were Bacillus licheniformis, Azonexus hydrophilusd, Azospira oryzae, Arobacter cloacae, Cellvibrio mixtus subsp. Mixtus, Bacillus tequilensis, Clostridium populeti and Clostridium xylanolyticum, respectively. 【Conclusion】Composite microbial community GF-20 sub-cultured under different conditions (temperature 4-30℃, pH 6.0-9.0) kept efficient corn stalk decomposing activity and stable community composition structure, a good application prospect should be anticipated.

Key words: decomposition of corn stalk, composite microbial community GF-20, low temperature, cellulose enzyme activity, composition stability

Qingge-er, GAO Ju-lin, YU Xiao-fang, HU Shu-ping, WANG Zhi-gang, WANG Zhen, Naoganchaolu BORJIGIN . Function and Composition Stability of a Composite Microbial System GF-20 with Efficient Corn Stalk Decomposition Under Low Temperature[J].Scientia Agricultura Sinica, 2016, 49(3): 443-454.

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Function and Composition Stability of a Composite Microbial System GF-20 with Efficient Corn Stalk Decomposition Under Low Temperature

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