IAA对水稻根毛形成与水通道蛋白基因表达关系的研究

doi:10.3864/j.issn.0578-1752.2015.21.004

Abstract

Abstract: 【Objective】To explore the effect of auxin (IAA) and its polar auxin transport carriers on the formation of rice root hair and genetic expression of aquaporins.【Method】The varieties of wild-type Japonica rice Zhonghua11 and its over-expressed OsPIN1a transgenic rice primary root were used in this paper. A small agar block which contains different concentrations of IAA and combination of IAA and inhibitors of polar auxin transport efflux carrier TIBA (2, 3, 5-Triiodobenzoic acid), NPA (N-1-naphthylphthalamic acid) or polar auxin transport influx carrier CHPAA (3-Chloro-4-hydroxyphenylacetic acid) was affixed to the lateral side of rice root tip when the primary root reached 0.5—1.0 cm in length in a aeroponic and dark culture. After a 12h treatment, the root hair length, root growth rate, root hair density and relative water content in rice roots were detected. The photos were taken by confocal laser scanning microscope (Zeiss Axiophot microscope with a Qimaging Retiga 1300 12-bit monochrome CCD camera with Open Lab v3.0.9) to observe subcellular localization of OsPIN1a-GFP in the tip and hair of the rice root. In addition, semi-quantitative RT-PCR was applied to detecting the OsPIN1a and aquaporins genes expression before and after the formation of the rice root hair. 【Result】The results showed that the length and density of the root hair enhanced correspondingly with an increase in IAA concentration range from 0 to 5.0 mg·L^-1, and 2.5 mg·L^-1 IAA had an optimal effect on inducing the formation of root hair. However, as IAA exceeds 5.0 mg·L^-1, the increase in root hair was insignificant and thus the primary root growth was severely inhibited. The new root hair only appeared when agar was attached to the meristematic zone of root tip but not formed in the ripe areas of the primary root. Combined treatment with IAA and lower concentration of TIBA or NPA did not affect formation of root hairs. In contrast, higher concentration of TIBA or NPA significantly inhibited its formation and elongation. However, much lower concentrations of CHPAA can significantly inhibit the formation and elongation of the root hair. In addition, IAA treatment increased genes expression of aquaporins as well as the formation of the root hair, thus improving the relative content of water in root tips. IAA treatment markedly improved the expression of OsPIN1a and the content of OsPIN1a-GFP in root tips and root hair.【Conclusion】It was suggested that the formation of rice root hair was induced by IAA and associated with the involvement of IAA efflux and influx transporters and influx process had the most significant effect. OsPIN1a played a key role in the formation and elongation of the root hair. IAA can promote genes expression of aquaporins and increase relative water content, thus alleviating water stress in rice roots in the aeroponic culture.

Key words: rice, root hair, auxin, polar auxin transportation, aquaporins

MO Yi-wei, LI Xia-jie, WANG Hai, CHEN Ze-kai, YANG Guo, WANG Wei. Effect of Auxin Treatment on Root Hair Formation and Aquaporins Genes Expression in Root Hair of Rice[J].Scientia Agricultura Sinica, 2015, 48(21): 4227-4239.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2015.21.004

https://www.chinaagrisci.com/EN/Y2015/V48/I21/4227

References

[1] Gilroy, Jones D L. Through form to function: Root hair development and nutrient uptake. Trends in Plant Science, 2000, 5(2): 56- 60.

[2] Grebe M. The patterning of epidermal hairs in Arabidopsis updated. Current Opinion in Plant Biology, 2012, 15(1): 31-37.

[3] Miller D D, de Ruijter N C A, Emons A M C. From signal to form: Aspects of the cytoskeleton-plasma membrane-cell wall continuum in root hair tips. Journal of Experimental Botany, 1997, 48(316): 1881-1896.

[4] 张德健, 夏仁学, 曹秀. 根毛的生长发育及其遗传基础. 植物生理学报, 2015, 51(1): 9-20.

Zhang D J, Xia R X, Cao X. Root hair development and its genetic basis. Plant Physiology Journal, 2015, 51(1): 9-20. (in Chinese)

[5] Kim C M, Dolan L. Root hair development involves asymmetric cell division in Brachypodium distachyon and symmetric division in Oryza sativa. New Phytologist, 2011, 192(3): 601-610.

[6] Huang J, Kim C M, Xuan Y, Liu J, Kim T H, Kim B K, Han C. Formin homology 1 (OsFH1) regulates root-hair elongation in rice (Oryza sativa). Planta, 2013, 237(5): 1227-1239.

[7] Rahman A, Hosokawa S, Oono Y, Amakawa T, Goto N, Tsurumi S. Auxin and ethylene response interactions during Arabidopsis root hair development dissected by auxin influx modulators. Plant Physiology, 2002, 130(4): 1908-1917.

[8] 王立德, 廖红, 王秀荣, 严小龙. 植物根毛的发生、发育及养分吸收. 植物学通报, 2004, 21(6): 649- 659.

Wang L D, Liao H, Wang X R, Yan X L. Root hair initiation and development and nutrient uptake in plants. Chinese Bulletin of Botany, 2004, 21(6): 649- 659. (in Chinese)

[9] Zhu C H, Gan L, Shen Z G, Xia K. Interactions between jasmonates and ethylene in the regulation of root hair development in Arabidopsis. Journal of Experimental Botany, 2006, 57(6): 1299-1308.

[10] 丁沃娜, 童艳丽, 吴晶, 朱世华. 一个水稻短根毛突变体的鉴定和基因定位. 中国农业科学, 2011, 44(21): 4333-4339.

Ding W N, Tong Y L, Wu J, Zhu S H. Identification and gene mapping of a novel short root hair mutant in rice. Scientia Agricultura Sinica, 2011, 44(21): 4333-4339. (in Chinese)

[11] Gu F, Nielsen E. Targeting and regulation of cell wall synthesis during tip growth in plants. Journal of Integrative Plant Biology, 2013, 55(9): 835-846.

[12] Won S K, Choi S B, Kumari S, Cho M, Lee S H, Cho H T. Root hair-specific EXPANSIN B genes have been selected for graminaceae root hairs. Molecules and Cells, 2010, 30(4): 369-376.

[13] Yu Z, Kang B, He X, Lü S, Bai Y, Ding W, Chen M, Cho H, Wu P. Root hair specific expansins modulate root hair elongation in rice. The Plant Journal, 2011, 66(5): 725-734.

[14] 马旭凤, 于涛, 汪李宏, 石喜, 郑灵祥, 王密侠, 姚雅琴, 蔡焕杰. 苗期水分亏缺对玉米根系发育及解剖结构的影响. 应用生态学报, 2010, 21(7): 1731-1736.

Ma X F, Yu T, Wang L H, Shi X, Zheng L X, Wang M X, Yao Y Q, Cai H J. Effects of water deficit at seedling stage on maize root development and anatomical structure. Chinese Journal of Applied Ecology, 2010, 21(7): 1731- 1736. (in Chinese)

[15] Niu Y, Jin C, Jin G, Zhou Q, Lin X, Tang C, Zhang Y. Auxin modulates the enhanced development of root hairs in Arabidopsis thaliana (L.) Heynh under elevated CO₂. Plant, Cell and Environment, 2011, 34(8): 1304-1317.

[16] Wang Y, Du S T, Li L L, Huang L D, Fang P, Lin X Y, Zhang Y S, Wang H L. Effect of CO₂elevation on root growth and its relationship with indole acetic acid and ethylene in tomato seedlings. Pedosphere, 2009, 19(5): 570-576.

[17] Zhu Z X, Liu Y, Liu S J, Mao C Z, Wu Y R, Wu Ping. A gain-of-function mutation in OsIAA11 affects lateral root development in rice. Molecular Plant, 2012, 5(1): 154-161.

[18] 李运合, 孙光明, 吴蓓. 植物生长素的极性运输载体研究进展. 西北植物学报, 2009, 29(8): 1714-1722.

Li Y H, Sun G M, Wu B. Advances on carriers of plant polar auxin transport. Acta Botanica Breali-Occidentalia Sinica, 2009, 29(8): 1714-1722. (in Chinese)

[19] Friml J, Vieten A, Sauer M, Weijers D, Schwarz H, Hamann T, Offringa R, Jürgens G. Efflux-dependent auxin gradients establish the apical-basal axis of Arabidopsis. Nature, 2003, 426 (6963): 147-153.

[20] Xu M, Zhu L, Shou H, Wu P. A PIN1 family gene, OsPIN1, involved in auxin-dependent adventitious root emergence and tillering in rice. Plant and Cell Physiology, 2005, 46: 1674-1681.

[21] Wang J R, Hu H, Wang G H, Li J, Chen J Y, Wu P. Expression of PIN genes in rice (Oryza sativa L.): Tissue specificity and regulation by hormones. Molecular Plant, 2009, 2(4): 823-831.

[22] Sakurai J, Ishikawa F, Yamaguchi T, Uemura M, Maeshima M. Identification of 33 rice aquaporin genes and analysis of their expression and function. Plant Cell Physiology, 2005, 46(9): 1568-1577.

[23] 郑小亚, 张正斌, 徐萍, 杨引福. 逆境条件下糯玉米水分利用效率和水通道蛋白表达差异研究. 中国生态农业学报, 2011,19(3): 607-613.

Zheng X Y, Zhang Z B, Xu Ping, Yang Y F. Differences in water use efficiency and aquaporin gene expression in waxy maize varieties under different stress conditions. Chinese Journal of Eco-Agriculture, 2011, 19(3): 607-613. (in Chinese)

[24] 李嵘, 牛向丽, 苗雁文, 熊方杰, 刘永胜. 水通道蛋白基因OsPIP2;6的功能分析. 中国农业科学. 2013, 46(15): 3079-3086.

Li R, Niu X L, Miao Y W, Xiong F J, Liu Y S. Functional characterization of the plasma intrinsic protein gene OsPIP2; 6 in rice. Scientia Agricultura Sinica, 2013, 46(15): 3079-3086. (in Chinese)

[25] Tyerman S D, Niemietz C M, Bramley H. Plant aquaporins: Multifunctional water and solute channels with expanding roles. Plant Cell and Environment, 2002, 25: 173-194.

[26] Xu H W, Mo Y W, Wang W, Wang H, Wang Z. OsPIN1a gene participates in regulating negative phototropism of rice roots. Rice Science, 2014, 21(2): 83-89.

[27] Mo Y, Liang G, Shi W, Xie J. Metabolic responses of alfalfa (Medicago Sativa L.) leaves to low and high temperature-induced stresses. African Journal of Biotechnology, 2011, 10(7): 1117-1124.

[28] 潘建伟, 叶晓帆, 王超, 涂世伟. 拟南芥PIN2介导的生长素极性运输调控植物根向地性. 浙江师范大学学报: 自然科学版, 2010, 33(1): 1- 6

Pan J W, Ye X F, Wang C, Tu S W. PIN2-mediated polar auxin transport regulation of root gravitropism in Arabidopsis thaliana. Journal of Zhejiang Normal University: Nature Sciences, 2010, 33(1): 1- 6. (in Chinese)

[29] Cao X, Chen C, Zhang D, Shu B, Xiao J, Xia R. Influence of nutrient deficiency on root architecture and root hair morphology of trifoliate orange (Poncirus trifoliata L. Raf.) seedlings under sand culture. Scientia Horticulturae, 2013, 162(10): 100-105.

[30] Jones A R, Kramer E M, Knox K, Swarup R, Bennett M J, Lazarus C M, Leyser H M, Grierson C S. Auxin transport through non-hair cells sustains root- hair development. Nature Cell Biology, 2008, 11(1): 78- 84.

[31] Guo K, Kong W W, Yang Z M. Carbon monoxide promotes root hair development in tomato. Plant and Cell Environment, 2009, 32(8): 1033-1045.

[32] Chen Y, Fan X, Song W, Zhang Y, Xu G. Over-expression of OsPIN2 leads to increased tiller numbers, angle and shorter plant height through suppression of OsLAZYl. Plant Biotechemistry Journal, 2012, 10(2): 139-149.

[33] Tamaki V, Mecier H. Cytokinin and auxin communicate nitrogen availability as long-distance signal molecules in pineapple (Ananas comosus). Journal of Plant Physiology, 2007, 164(11): 1543-1547.

[34] Forde B G. Local and long-range signaling pathways regulating plant responses to nitrate. Annual Review of Plant Biology, 2002, 53(2): 203- 224.

[35] Park H C, Cha J Y, Yun D J. Roles of YUCCAs in auxin biosynthesis and drought stress responses in plants. Plant Signaling and Behavior, 2013, 8(6): e24495.

[36] Kim J I , Baek D, Park H C, Chun H J, Oh D H, Lee M K, Cha J Y, Kim W Y, Kim M C, Chung W S, Bohnert H J, Lee S Y, Bressan R A, Lee S W, Yun D J. Overexpression of Arabidopsis YUCCA6 in potato results in high-auxin developmental phenotypes and enhanced resistance to water deficit. Molecular Plant, 2013, 6(2): 337-349.

[37] Zhang Y M, Yan Y S, Wang L N, Yang K, Xiao N, Liu Y F, Fu Y P, Sun Z X, Fang R X, Chen X Y. A novel rice gene, NRR responds to macronutrient deficiency and regulates root growth. Molecular Plant, 2012, 5(1): 63-72.

[38] Xu W, Jia L, Shi W, Liang J, Zhou F, Li Q, Zhang J. Abscisic acid accumulation modulates auxin transport in the root tip to enhance proton secretion for maintaining root growth under moderate water stress. New Phytologist, 2013, 197(1): 139-150.

[39] Sakurai-Ishikawa J, Murai-Hatano M, Hayashi H, Ahamed A, Fukushi K, Matsumoto, Kitagawa Y. Transpiration from shoots triggers diurnal changes in root aquaporin expression. Plant, Cell and Environment , 2011, 34(7): 1150-1163.

[40] Guo L, Wang Z Y, Lin H, Cui W E, Chen J, Liu M, Chen Z L, Qu L J, Gu H. Expression and function alanalysis of the rice plasma membrane intrinsic protein gene family. Cell Research, 2006, 16(3): 277-286.

[41] Yin Y X, Wang S B, Xiao H J, Zhang H X, Zhang Z, Jing H, Zhang Y L, Chen R G, Gong Z H. Overexpression of the CaTIP1-1 pepper gene in tobacco enhances resistance to osmotic stresses. International Journal of Molecular Sciences, 2014, 15(11): 20101-20116.

[42] Liu C, Fukumoto T, Matsumoto T, Gena P, Frascaria D, Kaneko T, Katsuhara M, Zhong S, Sun X, Zhu Y, Iwasaki I, Ding X, Calamita G, Kitagawa Y. Aquaporin OsPIP1:1 promotes rice salt resistance and seed germination. Plant Physiology and Biochemistry, 2013, 63(2): 151-158.

[43] Péret B, Li G, Zhao J, Band L R, Voß U, Postaire O, Luu D T, Da Ines O, Casimiro I, Lucas M, Wells D M, Lazzerini L, Nacry P, King J R, Jensen O E, Schäffner A R, Maurel C, Bennett M J. Auxin regulates aquaporin function to facilitate lateral root emergence. Nature Cell Biology, 2012, 14(10): 991-999.

Related Articles 15

[1]	XIAO DeShun, XU ChunMei, WANG DanYing, ZHANG XiuFu, CHEN Song, CHU Guang, LIU YuanHui. Effects of Rhizosphere Oxygen Environment on Phosphorus Uptake of Rice Seedlings and Its Physiological Mechanisms in Hydroponic Condition [J]. Scientia Agricultura Sinica, 2023, 56(2): 236-248.
[2]	ZHANG XiaoLi, TAO Wei, GAO GuoQing, CHEN Lei, GUO Hui, ZHANG Hua, TANG MaoYan, LIANG TianFeng. Effects of Direct Seeding Cultivation Method on Growth Stage, Lodging Resistance and Yield Benefit of Double-Cropping Early Rice [J]. Scientia Agricultura Sinica, 2023, 56(2): 249-263.
[3]	ZHANG Wei,YAN LingLing,FU ZhiQiang,XU Ying,GUO HuiJuan,ZHOU MengYao,LONG Pan. Effects of Sowing Date on Yield of Double Cropping Rice and Utilization Efficiency of Light and Heat Energy in Hunan Province [J]. Scientia Agricultura Sinica, 2023, 56(1): 31-45.
[4]	FENG XiangQian,YIN Min,WANG MengJia,MA HengYu,CHU Guang,LIU YuanHui,XU ChunMei,ZHANG XiuFu,ZHANG YunBo,WANG DanYing,CHEN Song. Effects of Meteorological Factors on Quality of Late Japonica Rice During Late Season Grain Filling Stage Under ‘Early Indica and Late Japonica’ Cultivation Pattern in Southern China [J]. Scientia Agricultura Sinica, 2023, 56(1): 46-63.
[5]	SANG ShiFei,CAO MengYu,WANG YaNan,WANG JunYi,SUN XiaoHan,ZHANG WenLing,JI ShengDong. Research Progress of Nitrogen Efficiency Related Genes in Rice [J]. Scientia Agricultura Sinica, 2022, 55(8): 1479-1491.
[6]	GUI RunFei,WANG ZaiMan,PAN ShengGang,ZHANG MingHua,TANG XiangRu,MO ZhaoWen. Effects of Nitrogen-Reducing Side Deep Application of Liquid Fertilizer at Tillering Stage on Yield and Nitrogen Utilization of Fragrant Rice [J]. Scientia Agricultura Sinica, 2022, 55(8): 1529-1545.
[7]	LIAO Ping,MENG Yi,WENG WenAn,HUANG Shan,ZENG YongJun,ZHANG HongCheng. Effects of Hybrid Rice on Grain Yield and Nitrogen Use Efficiency: A Meta-Analysis [J]. Scientia Agricultura Sinica, 2022, 55(8): 1546-1556.
[8]	HAN XiaoTong,YANG BaoJun,LI SuXuan,LIAO FuBing,LIU ShuHua,TANG Jian,YAO Qing. Intelligent Forecasting Method of Rice Sheath Blight Based on Images [J]. Scientia Agricultura Sinica, 2022, 55(8): 1557-1567.
[9]	GAO JiaRui,FANG ShengZhi,ZHANG YuLing,AN Jing,YU Na,ZOU HongTao. Characteristics of Organic Nitrogen Mineralization in Paddy Soil with Different Reclamation Years in Black Soil of Northeast China [J]. Scientia Agricultura Sinica, 2022, 55(8): 1579-1588.
[10]	ZHU DaWei,ZHANG LinPing,CHEN MingXue,FANG ChangYun,YU YongHong,ZHENG XiaoLong,SHAO YaFang. Characteristics of High-Quality Rice Varieties and Taste Sensory Evaluation Values in China [J]. Scientia Agricultura Sinica, 2022, 55(7): 1271-1283.
[11]	ZHAO Ling, ZHANG Yong, WEI XiaoDong, LIANG WenHua, ZHAO ChunFang, ZHOU LiHui, YAO Shu, WANG CaiLin, ZHANG YaDong. Mapping of QTLs for Chlorophyll Content in Flag Leaves of Rice on High-Density Bin Map [J]. Scientia Agricultura Sinica, 2022, 55(5): 825-836.
[12]	JIANG JingJing,ZHOU TianYang,WEI ChenHua,WU JiaNing,ZHANG Hao,LIU LiJun,WANG ZhiQin,GU JunFei,YANG JianChang. Effects of Crop Management Practices on Grain Quality of Superior and Inferior Spikelets of Super Rice [J]. Scientia Agricultura Sinica, 2022, 55(5): 874-889.
[13]	ZHANG YaLing, GAO Qing, ZHAO Yuhan, LIU Rui, FU Zhongju, LI Xue, SUN Yujia, JIN XueHui. Evaluation of Rice Blast Resistance and Genetic Structure Analysis of Rice Germplasm in Heilongjiang Province [J]. Scientia Agricultura Sinica, 2022, 55(4): 625-640.
[14]	WANG YaLiang,ZHU DeFeng,CHEN RuoXia,FANG WenYing,WANG JingQing,XIANG Jing,CHEN HuiZhe,ZHANG YuPing,CHEN JiangHua. Beneficial Effects of Precision Drill Sowing with Low Seeding Rates in Machine Transplanting for Hybrid Rice to Improve Population Uniformity and Yield [J]. Scientia Agricultura Sinica, 2022, 55(4): 666-679.
[15]	CHEN TingTing, FU WeiMeng, YU Jing, FENG BaoHua, LI GuangYan, FU GuanFu, TAO LongXing. The Photosynthesis Characteristics of Colored Rice Leaves and Its Relation with Antioxidant Capacity and Anthocyanin Content [J]. Scientia Agricultura Sinica, 2022, 55(3): 467-478.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

Effect of Auxin Treatment on Root Hair Formation and Aquaporins Genes Expression in Root Hair of Rice

RichHTML

PDF

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0