IAA对水稻根毛形成与水通道蛋白基因表达关系的研究

doi:10.3864/j.issn.0578-1752.2015.21.004

Abstract

Abstract: 【Objective】To explore the effect of auxin (IAA) and its polar auxin transport carriers on the formation of rice root hair and genetic expression of aquaporins.【Method】The varieties of wild-type Japonica rice Zhonghua11 and its over-expressed OsPIN1a transgenic rice primary root were used in this paper. A small agar block which contains different concentrations of IAA and combination of IAA and inhibitors of polar auxin transport efflux carrier TIBA (2, 3, 5-Triiodobenzoic acid), NPA (N-1-naphthylphthalamic acid) or polar auxin transport influx carrier CHPAA (3-Chloro-4-hydroxyphenylacetic acid) was affixed to the lateral side of rice root tip when the primary root reached 0.5—1.0 cm in length in a aeroponic and dark culture. After a 12h treatment, the root hair length, root growth rate, root hair density and relative water content in rice roots were detected. The photos were taken by confocal laser scanning microscope (Zeiss Axiophot microscope with a Qimaging Retiga 1300 12-bit monochrome CCD camera with Open Lab v3.0.9) to observe subcellular localization of OsPIN1a-GFP in the tip and hair of the rice root. In addition, semi-quantitative RT-PCR was applied to detecting the OsPIN1a and aquaporins genes expression before and after the formation of the rice root hair. 【Result】The results showed that the length and density of the root hair enhanced correspondingly with an increase in IAA concentration range from 0 to 5.0 mg·L^-1, and 2.5 mg·L^-1 IAA had an optimal effect on inducing the formation of root hair. However, as IAA exceeds 5.0 mg·L^-1, the increase in root hair was insignificant and thus the primary root growth was severely inhibited. The new root hair only appeared when agar was attached to the meristematic zone of root tip but not formed in the ripe areas of the primary root. Combined treatment with IAA and lower concentration of TIBA or NPA did not affect formation of root hairs. In contrast, higher concentration of TIBA or NPA significantly inhibited its formation and elongation. However, much lower concentrations of CHPAA can significantly inhibit the formation and elongation of the root hair. In addition, IAA treatment increased genes expression of aquaporins as well as the formation of the root hair, thus improving the relative content of water in root tips. IAA treatment markedly improved the expression of OsPIN1a and the content of OsPIN1a-GFP in root tips and root hair.【Conclusion】It was suggested that the formation of rice root hair was induced by IAA and associated with the involvement of IAA efflux and influx transporters and influx process had the most significant effect. OsPIN1a played a key role in the formation and elongation of the root hair. IAA can promote genes expression of aquaporins and increase relative water content, thus alleviating water stress in rice roots in the aeroponic culture.

Key words: rice, root hair, auxin, polar auxin transportation, aquaporins

MO Yi-wei, LI Xia-jie, WANG Hai, CHEN Ze-kai, YANG Guo, WANG Wei. Effect of Auxin Treatment on Root Hair Formation and Aquaporins Genes Expression in Root Hair of Rice[J].Scientia Agricultura Sinica, 2015, 48(21): 4227-4239.

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