Scientia Agricultura Sinica ›› 2015, Vol. 48 ›› Issue (10): 2007-2016.doi: 10.3864/j.issn.0578-1752.2015.10.013

• HORTICULTURE • Previous Articles     Next Articles

Analysis of Expression Levels of Floral Genes in the Buds on Different Branch Nodes of Grapevine

LIU Dan1, SUN Xin1, MU Qian1, WU Wei-min2, ZHANG Zhen1, FANG Jing-gui1   

  1. 1College of Horticulture, Nanjing Agricultural University, Nanjing 210095
    2Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014
  • Received:2014-12-04 Online:2015-05-16 Published:2015-05-16

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Abstract: 【Objective】 The objective of the paper is to study the expression pattern of eight genes in different nodes and the phenological period of buds development, and explore the mechanism of the time and speed of buds development on different nodes of grape branches.【Method】 In this study, the expression of eight floral genes (VvFT, VvSOC1, VvAP1, VvAP2, VvAP3, VvFUL, VvAG and VvFLC) in the buds at different branch nodes of 8-year-old ‘Fujiminori’ grapevine was examined by real-time quantitative PCR, and the phenological period of flower bud differentiation was observed. 【Result】The floral differentiation of ‘Fujiminori’ grapevine showed a remarkable characteristics of ‘beyond section’. The first bud began to form and develop from late April or early May, and the formation time of the last bud at the top was in late September. The development time of the lower nodes was five months longer than the upper nodes, but the flowering time of them was almost at the same in the second season. The buds in the upper position differentiated from the bottom upwards to the top gradually, and the process of bud differentiation at the top was shorter than that at the bottom. The expression of floral genes in buds at different branch nodes was different. The expression level of VvFT was low and showed no significant difference during the growing season. VvSOC1 , VvAP2, VvAP3 and VvFUL. The highest expression level appeared in the early period of bud differentiation, and then decreased over time. Buds on middle nodes undergo a low speed differentiation process for a long time, and the expression of floral genes kept a high level during the differentiation period. By contrast, the buds on upper and lower nodes differentiated at a higher speed, and the expression of floral genes was lower. In the end, the development of the buds on different nodes reached the similar level. 【Conclusion】 The expression levels are different on different nodes, and high expression periods were also different on different nodes. Although the peak period of floral development of lower and upper buds were relatively late, continuous development was observed in these nodes, which led to the floral differentiation simultaneously on the lower, middle and upper nodes. This may be one of the factors that lead to difference of differentiation quality between buds on different nodes.gene in the buds at different branch nodes expressed highly during all the growing season, and showed a similar expression trend. The expression of VvAP1, VvAP2, VvAP3, and VvFUL genes in the buds at lower and upper nodes showed no remarkable change, however on the middle nodes, the expression of these genes changed greatly, and appeared an obvious upgrade firstly then descending latter tendency, what’s more, in the buds on 8, 11 and 15th nodes, the expression of these genes was higher than that at other nodes. VvAG showed different expression patterns with VvAP1

Key words: grape, bud, different nodes, gene expression

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