水稻矮化并花发育异常突变体dwarf and deformed flower 2 (ddf2)的基因定位与候选基因分析

doi:10.3864/j.issn.0578-1752.2015.10.001

Abstract

Abstract: 【Objective】A rice mutant, dwarf and deformed flower 2 (ddf2) , was characterized in the present paper. These results will provide a foundation for map-based cloning and functional analysis of the DDF2 gene. 【Method】 The ddf2 mutant was derived from tissue culture mutation generations of indica restorer 602. At the heading stage, the plant height, panicle length, width and length of leaves and internodes of the ddf2 mutant and wild type were measured. Moreover, the phenotype and histology analyses of stem, leaf and spikelet were conducted. A population containing 1 024 F₂ mutants from the zhonghua11/ddf2 cross was used to map the DDF2 gene. The expression of candidate genes were detected by real time PCR. 【Result】 The leaves and internodes of ddf2 mutant were all much shorter and thinner than those of the wild type, and the panicleof ddf2 mutant was shorter than that of the wild type. According to the histology analysis, the width between two large veins of leaf was decreased dramatically in ddf2 mutant, while no obvious difference in the numbers of large and small veins was observed between the ddf2 mutant and the wild type. Further analysis showed that the size and number of mesophyll cells in ddf2 leaves were all reduced significantly. Furthermore, parenchyma cells were significantly reduced in size and number in ddf2 stems, while there was no difference in the number of vascular bundles in stem between the ddf2 mutant and the wild type. These results indicated both the cell division and cell expansion were inhibited in ddf2 leaves and stems. Additionally, the spikelet and floral organs in ddf2 mutant display serious defects. In ddf2 florets, the lemmas were distorted while the paleas were degraded in whorl 1. The stamens were degenerated seriously or transformed into pistil-like organs in whorl 3. Elongated sterile lemma and extra florets were often observed in some ddf2 spikelets. Genetic analysis indicated that ddf2 was a nuclear recessive gene. By using 1 024 F₂ mutants from the zhonghua11/ddf2 cross, the DDF2 gene was mapped between In/Del markers S-11 and S-14 on the short arm of chromosome 11 near the centromere, with genetic distance of 0.049 cM and 0.098 cM, respectively, and an approximate physical distance of 90.295 kb. The gene was co-segregating with another In/Del marker S-24. There were 12 MSU annotation genes within the mapping region. Based on the qRT-RCR, it was found that the expression of LOC_Os11g17600, encoding an exocyst complex component Sec3_C protein, was down regulated in the ddf2 mutant. Then, the LOC_Os11g17600 was considered as candidate gene of DDF2.【Conclusion】DDF2 is considered to be a novel gene controlling both stem, leaf and floral organ development.

Key words: rice (Oryza Sativa), dwarf, floral organ, dwarf and deformed flower 2 (ddf2), gene mapping

ZHANG Ling, GUO Shuang, WANG Ling, ZHANG Tian-quan, ZHUANG Hui, LONG Yu-chen, HE Guang-hua, LI Yun-feng. Gene Mapping and Candidate Gene Analysis of a dwarf and deformed flower 2 (ddf2) Mutant in Rice (Oryza sativa)[J].Scientia Agricultura Sinica, 2015, 48(10): 1873-1881.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2015.10.001

https://www.chinaagrisci.com/EN/Y2015/V48/I10/1873

References

[1] Coen E S, Meyerowitz E M. The war of the whorls: Genetic interactions controlling flower development. Nature, 1991, 353(6339): 31-37.

[2] Weigel D, Meyerowitz E M. The ABCs of floral homeotic genes. Cell, 1994, 78(2): 203-209.

[3] Ditta G, Pinyopich A, Robles P, Pelaz S, Yanofsky M F. The SEP4 Gene of Arabidopsis thaliana functions in floral organ and meristem identity. Current Biology, 2004, 14(21): 1935-1940.

[4] Nagasawa N, Miyoshi M, Sano Y, Satoh H, Sakai H, Nagato Y. SUPERWOMAN1 and DROOPING LEAF genes control floral organ identity in rice. Development, 2003, 130(4): 705-718.

[5] Yao S G, Ohmori S, Kimizu M, Yoshida H. Unequal genetic redundancy of rice PISTILLATA orthologs, OsMADS2 and OsMADS4, in lodicule and stamen development. Plant and Cell Physiology, 2008, 49(5): 853-857.

[6] Yamaguchi T, Lee D Y, Miyao A, Hirochika H, An G H, Hirano H Y. Functional diversification of the two C-class MADS box genes OsMADS3 and OsMADS58 in Oryza sativa18(1): 15-28.. The Plant Cell, 2006,

[7] Yamaguchi T, Nagasawa N, Kawasaki S, Matsuoka M, Nagato Y, Hirano H. The YABBY gene DROOPING LEAF regulates carpel specification and midrib development in Oryza sativa. The Plant Cell, 2004, 16(2): 500-509.

[8] Sanchez-Corrales Y E, Alvarez-Buylla E R, Mendoza L. The Arabidopsis thaliana flower organ specification gene regulatory network determines a robust differentiation process. Journal of Theoretical Biology, 2010, 264(1): 971-983.

[9] Ikeda K, Ito M, Nagasawa N, Kyozuka J, Nagato Y. Rice ABERRANT PANICLE ORGANIZATION 1, encoding an F-box protein, regulates meristem fate. The Plant Journal, 2007, 51(6): 1030-1040.

[10] Ikeda-Kawakatsu K, Yasuno N, Oikawa T, Iida S, Nagato Y, Maekawa M, Kyozuka J. Expression level of ABERRANT PANICLE ORGANIZATION1 determines rice inflorescence form through control of cell proliferation in the meristem. Plant Physiology, 2009, 150(2): 736-747.

[11] Xiao H, Tang J F, Li Y F, Wang W M, Li X B, Jin L, Xie R, Luo H F, Zhao X F, Meng Z, He G H, Zhu L H. STAMENLESS 1, encoding a single C2H2 zinc finger protein, regulates floral organ identity in rice. The Plant Journal, 2009, 59(5): 789-801.

[12] Sang X C, Li Y F, Luo Z K, Ren D Y, Fang L K, Wang N, Zhao F M, Ling Y H, Yang Z L, Liu Y S, He G H. CHIMERIC FLORAL ORGANS1, encoding a monocot-specific MADS box protein, regulates floral organ identity in rice. Plant Physiology, 2012, 160(2): 788-807.

[13] Grennan A K. Gibberellin metabolism enzymes in rice. Plant Physiology, 2006, 141(2): 524-526.

[14] Fan L M, Feng X Y, Wang Y, Deng X W. Gibberellin signal transduction in rice. Journal of Integrative Plant Biology, 2007, 49(6): 731-741.

[15] Kim T W, Wang Z Y. Brassinosteroid signal transduction from receptor kinases to transcription factors. Annual Reviews Compilations: Plant Hormones, 2010, 61: 681-704.

[16] Tong H N, Chu C C. Brassinosteroid signaling and application in rice. Journal of Genetics and Genomics, 2012, 39(1): 3-9.

[17] Sato Y, Miura Y, Hirochika H, Kitano H, Matsuoka M, Sentoku N. Loss-of-function mutations in the rice homeobox gene OSH15 affect the architecture of internodes resulting in dwarf plants. The EMBO Journal, 1999, 18(4): 992-1002.

[18] Wei X J, Xu J F, Guo H N, Jiang L, Chen S H, Yu C Y, Zhou Z L, Hu P S, Zhai H Q, Wan J M. DTH8 suppresses flowering in rice, influencing plant height and yield potential simultaneously. Plant Physiology, 2010, 153(4): 1747-1758.

[19] Yang W B, Ren S L, Zhang X M, Gao M J, Qi Y B, Wang J, Li Q, He Z H. BENT UPPERMOST INTERNODE1 encodes the class II formin FH5 crucial for actin organization and rice development. The Plant Cell, 2011, 23(2): 661-680.

[20] Zhang Z, Zhang Y, Tan H X, Li G, Yuan Z, Zhang D B. RICE MORPHOLOGY DETERMINANT encodes the type II formin FH5 and regulates rice morphogenesis. The Plant Cell, 2011, 23(2): 681-700.

[21] Duan Y L, Li S P, Chen Z W, Zheng L L, Diao Z J, Zhou Y C, Lan T, Guan H Z, Pan R S, Xue Y B, Wu W R. Dwarf and deformed flower 1, encoding an F-box protein, is critical for vegetative and floral development in rice (Oryza sativa L.). The Plant Journal, 2012, 72(5): 829-842.

[22] Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregation analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proceedings of the National Academy of Sciences of the United States of America, 1991, 88(21): 9828-9832.

[23] Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research, 1980, 8(19): 4321-4325.

[24] Munson M, Novick P. The exocyst defrocked, a framework of rods revealed. Nature Structural & Molecular Biology, 2006, 13: 577-581.

[25] Wen T J, Schnable P S. Analyses of mutants of three genes that influence root hair development in Zea mays (Gramineae) suggest that root hairs are dispensable. American Journal of Botany, 1994, 81(7): 833-842.

[26] Wen T J, Hochholdinger F, Sauer M, Bruce W, Schnable P. The roothairless1 gene of maize encodes a homolog of sec3, which is involved in polar exocytosis. Plant Physiology, 2005, 138(3): 1637-1643.

[27] Brown P T H, Kyozuka J, Sukekiyo Y, Kimura Y, Shimamoto K, Lorz H. Molecular changes in protoplast-derived rice plants. Molecular and General Genetics, 1990, 223(2): 324-328.

[28] Kaeppler S M, Phillips R L. Tissue culture-induced DNA methylation variation in maize. Proceedings of the National Academy of Sciences of the United States of America, 1993, 90(19): 8773-8776.

[29] Krizova K, Fojtova M, Depicker A, Kovarik A. Cell culture-induced gradual and frequent epigenetic reprogramming of invertedly repeated tobacco transgene epialleles. Plant Physiology, 2009, 149(3): 1493-1504.

Related Articles 15

[1]	LI XiaoYing, WU JunKai, WANG HaiJing, LI MengYuan, SHEN YanHong, LIU JianZhen, ZHANG LiBin. Characterization of Volatiles Changes in Chinese Dwarf Cherry Fruit During Its Development [J]. Scientia Agricultura Sinica, 2021, 54(9): 1964-1980.
[2]	DIAO WeiNan,YUAN PingLi,GONG ChengSheng,ZHAO ShengJie,ZHU HongJu,LU XuQiang,HE Nan,YANG DongDong,LIU WenGe. Genetic Analysis and Gene Mapping of Canary Yellow in Watermelon Flesh [J]. Scientia Agricultura Sinica, 2021, 54(18): 3945-3958.
[3]	XU XinYang,SHEN Jia,ZHANG YueJian,LI GuoJing,NIU XiaoWei,SHOU WeiSong. Fine Mapping of an Immature Rind Color Gene GR in Melon [J]. Scientia Agricultura Sinica, 2021, 54(15): 3308-3319.
[4]	ZHANG Shuo,ZHI Hui,TANG ChanJuan,LUO MingZhao,TANG Sha,JIA GuanQing,JIA YanChao,DIAO XianMin. Cytological Characters Analysis and Low-Resolution Mapping of Stripe-Leaf MutantA36-S in Foxtail Millet [J]. Scientia Agricultura Sinica, 2021, 54(14): 2952-2964.
[5]	MA Jian, LI CongCong, HUANG YaTing, XIE YuLi, CHENG LingLing, WANG JianShe. Fine Mapping and Candidate Gene Analysis of Seed Coat Color Gene CmSC1 in Melon [J]. Scientia Agricultura Sinica, 2021, 54(10): 2167-2178.
[6]	ZHU XingHao,CHEN Qing,SHAO BingHao,GUO YuJun,ZHANG XiangLi,DU PengFei,ZHU Yao,HUANG YanQun,CHEN Wen. Effect of the Heterozygous Sex-Linked Dwarf Gene on Fat Deposition in Normal Type Chickens [J]. Scientia Agricultura Sinica, 2021, 54(1): 213-223.
[7]	Jian MA,CongCong LI,JianShe WANG. Fine Mapping and Candidate Gene Analysis of a Short Internodes Gene Cmdm1 in Melon (Cucumis melo L.) [J]. Scientia Agricultura Sinica, 2020, 53(4): 802-810.
[8]	DUAN YouHou,LU Feng. Genetic Analysis on Growth Period and Plant Height Traits of Early-maturing Dwarf Sorghum Male-Sterile Line P03A [J]. Scientia Agricultura Sinica, 2020, 53(14): 2828-2839.
[9]	LIANG HuiZhen,XU LanJie,DONG Wei,YU YongLiang,YANG HongQi,TAN ZhengWei,LI Lei,LIU XinMei. Mixed Inheritance Analysis and QTL Mapping for γ-Tocopherol Content in Soybean [J]. Scientia Agricultura Sinica, 2020, 53(11): 2149-2160.
[10]	LI MinJi,ZHANG Qiang,LI XingLiang,ZHOU BeiBei,YANG YuZhang,ZHANG JunKe,ZHOU Jia,WEI QinPing. Effects of 4 Dwarfing Rootstocks on Growth, Yield and Fruit Quality of ‘Fuji’ Sapling in Apple Replant Orchard [J]. Scientia Agricultura Sinica, 2020, 53(11): 2264-2271.
[11]	GONG ChengSheng, ZHAO ShengJie, LU XuQiang, HE Nan, ZHU HongJu, DOU JunLing, YUAN PingLi, LI BingBing, LIU WenGe. Chemical Compositions and Gene Mapping of Wax Powder on Watermelon Fruit Epidermis [J]. Scientia Agricultura Sinica, 2019, 52(9): 1587-1600.
[12]	HUANG ShengCai,WANG Bing,XIE GuoQiang,LIU ZhongLai,ZHANG MeiJuan,ZHANG ShuQing,CHENG XianGuo. Enrichment Profile of GA4 is an Important Regulatory Factor Triggering Rice Dwarf [J]. Scientia Agricultura Sinica, 2019, 52(5): 786-800.
[13]	BAI TuanHui,LI Li,ZHENG XianBo,WANG MiaoMiao,SONG ShangWei,JIAO Jian,SONG ChunHui. Screening and Expression Analysis of Co Candidate Genes in Columnar Apple [J]. Scientia Agricultura Sinica, 2019, 52(23): 4350-4363.
[14]	SONG Xi, PU DingFu, TIAN LuShen, YU QingQing, YANG YuHeng, Dai BingBing, ZHAO ChangBin, HUANG ChengYun, DENG WuMing. Genetic Analysis and Characterization of Hormone Response of Semi-Dwarf Mutant dw-1 in Brasscia napus L. [J]. Scientia Agricultura Sinica, 2019, 52(10): 1667-1677.
[15]	XIE Jia, ZHANG XiaoBo, TAO YiRan, XIONG YuZhen, ZHOU Qian, SUN Ying, YANG ZhengLin, ZHONG BingQiang, SANG XianChun. Identification and Gene Mapping of a Shorten Panicle and Seed Mutant sps1 in Rice (Oryza sativa L.) [J]. Scientia Agricultura Sinica, 2018, 51(9): 1617-1626.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

Gene Mapping and Candidate Gene Analysis of a dwarf and deformed flower 2 (ddf2) Mutant in Rice (Oryza sativa)

RichHTML

PDF