Scientia Agricultura Sinica ›› 2015, Vol. 48 ›› Issue (7): 1370-1382.doi: 10.3864/j.issn.0578-1752.2015.07.12

• HORTICULTURE • Previous Articles     Next Articles

Analysis of Phenolic Compounds in Red Grape Varieties

ZHANG Juan1, WANG Xiao-yu1, TIAN Cheng-rui1, ZHAO Qi-feng2, MA Xiao-he2, TANG Xiao-ping2, MA Ting-ting1, MA Jin-jin1   

  1. 1College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710061
    2Pomology Institute, Shanxi Academy of Agricultural Sciences, Taigu 030801, Shanxi
  • Received:2014-09-09 Online:2015-04-01 Published:2015-04-01

Abstract: 【Objective】 Phenols are the most important substances of wine, which not only affect the sensory quality of wine, but also determine the function of the physiological activity of wine. The contents and types of phenolic compounds, as well as the differences of phenolic compounds in different parts of grape fruits in 20 red wine grape varieties, which were taken from areas of the Shanxi Loess Plateau, were studied in order to acquire more effective improvement in wine making process, and provide a theoretical basis for the breeding of wine grapes. 【Method】 Using 20 red grape varieties collected from Pomology Institute of Shanxi Academy of Agricultural Sciences, grape resources garden (Taigu) as materials, berry size and its shape index, mass and percentage of different parts, soluble solids content (TSS), titratable acid content, TPC, TFA, CGE and monomer phenol content were measured. 【Result】 The result indicated that the fruit shape index of 20 grape varieties were higher than 0.95, and the fruit shape was oval or conical. There was a significant difference between the proportion of grape peels, seeds and pulps. The ‘Cabernet Sauvignon’ with a higher quality was the first cultivar in China, its proportion of peels and seeds were the highest and reached 31.3%; whereas the proportion of peels and seeds of ‘Cabernet Gernischet’ was lower, only 10.62%, meanwhile the wine fermented by ‘Cabernet Gernischet’ had a lower content of polyphenols and a supple texture. Titratable acid content of 20 varieties of grapes was 3.97-9.00 g·L-1 and the content of soluble solid was 14.5-25.4 g·L-1. Different grape varieties and different parts of the grape fruit (skin, seed and pulp) were significantly different in the polyphenols content. TPC (total phenolic content) of grape peel was 12-125 mg·100g-1FW (fruit fresh weight), TFA (total flavanol content) was 1-27 mg·100g-1FW; TPC of grape seed was 17-456 mg·100g-1 FW, TFA was 22-505 mg·100g-1FW, TPC of grape pulps was 1.24-3.24 mg·100g-1FW, that TPC in grape seed was 3-4 times of grape peel, TFA in the seed was 10 times more than the peel. Anthocyanin mainly existed in grape peels and the content of anthocyanin of grape peels was 1-34 mg·100g-1FW. Among the 20 varieties of grape, ‘Cabernet sauvignon’ exhibited the highest anthocyanin content, which was followed by ‘Ruby cabernet’, ‘Marselan’ and ‘Cabernet gernischet’. The grape cultivars such as ‘Marselan’ and ‘Cabernet sauvignon’ had higher phenolic compounds, meanwhile the grape cultivars such as ‘Cabernet gernischet’, ‘Heihuxiang’ and ‘Cinsaut cehco’ had lower phenols content. The content of phenolic compounds in different parts of various grapes was significantly different. The order was that grape seeds was the highest, followed by the grape skins, and the pulp was the lowest. The phenolic compounds content in pulp was much lower than that in skins and seeds, and pulp mainly contained phenolic acids. A total of 12 kinds of monomeric phenols in grape pulps were identified by HPLC, including 4 kinds of flavonoids, 7 kinds of phenolic acids and resveratrol. 【Conclusion】 Characters of grape affected not only the quality of wine, but also the phenolic compounds in wine. The final content of phenolic compounds in wine was determined by the size of fruit, the proportion between peels and seeds, the phenolic content and variety of grape peels, seeds and pulps. By controlling the polyphenols content of the grape skin, seed and pulp through wine making technology, the quality of red wine can be improved. Based on the characteristics of grape polyphenols, a selective grape breeding work should be carried out.

Key words: grape, varietal characteristic, phenolic compounds, brewing

[1]    Bravo L. Polyphenols: chemistry, dietary sources, metabolism, and nutritional significance. Nutrition Research, 1998, 56: 317-333.
[2]    Li H, Wang X Y, Li Y, Li P H, Wang H. Polyphenolic compounds and antioxidant properties of selected China wines. Food Chemistry, 2009, 112: 454-460.
[3]    Ma T T, Sun X Y, Gao G T, Wang X Y, Liu X Y, Du G R, Zhan J C. Phenolic characterisation and antioxidant capacity of young wines made from different grape varieties grown in Helanshan Donglu Wine Zone(China). South African Journal for Enology and Viticulture, 2014, 35(2), 321-331.
[4]    Stojanovic J, Silva J L. Influence of osmotic concentration, continuous high frequency ultrasound and dehydration on antioxidants, colour and chemical properties of rabbit eye blueberries. Food Chemistry, 2007, 101, 898-906.
[5]    Li Z, Pan Q H, Jin Z M, Mu L, Duan C Q. Comparison on phenolic compounds in Vitis vinifera cv. Cabernet Sauvignon wines from five wine-growing regions in China. Food Chemistry, 2011, 125(1): 77-83.
[6]    Fang F, Li J M, Pan Q H, Huang W D. Determination of red wine flavonoids by HPLC and effect of aging. Food Chemistry, 2007, 101, 428-433.
[7]    孙翔宇. 单品种葡萄酒, 商业葡萄酒酚类物质分析及发酵时去籽时间对葡萄酒中酚类物质含量影响[D]. 陕西西安: 陕西师范大学. 2013.
Sun X Y. Single-varietal wines, wine polyphenols during business analysis and seeded time of fermentation in wine polyphenols affect the content of the material[D]. Shaanxi, Xi’an: Shaanxi Normal University. 2013. (in Chinese)
[8]    朱林.中国酿酒葡萄的现状及栽培技术进展. [2006-3-18]. http://ptjwinehouse.vip.myrice.com.
Zhu L. Status and Progress of Chinese wine grape cultivation. [2006-3-18]. http:// ptjwinehouse. vip. myrice. com. (in Chinese)
[9]    于贞, 赵光鳌, 李记明. 葡萄皮中的酚类物质对葡萄酒中酚含量的影响. 酿酒科技, 2010(4): 46-51.
Yu Z, Zhao G A, Li J M. Effects of phenolic compounds in grape skin on phenol content in grape wine. Liquer-Making Science &Technology, 2010(4): 46-51. (in Chinese)
[10]   Spagna G, Barbagallo R N, Todaro A. A method for anthocyanin extraction from fresh grape skin. Food Science, 2003, 15(3): 337- 346.
[11]   Negro C, Tommasi L, Miceli A. Phenolic compounds and antioxidant activity from red grape marc extracts. Bioresource Technology, 2003, 87: 41-44.
[12]   马海燕, 张振文, 汪成东. 葡萄皮花色素不同提取方法的比较研究. 酿酒科技, 2005(5): 81-84.
Ma H Y, Zhang Z W, Wang C D. Comparison of different extracting methods of anthocyanidins from grape skins. Liquer-Making Science &Technology, 2005(5): 81-84. (in Chinese)
[13]   Liang Z C, W u B H, Fan P G, Yang C X, Duan W, Zheng X B, Liu C Y, Li S H. Anthocyanin composition and content in grape berry skin in Vitis germplasm. Food Chemistry, 2008, 111(4): 837-844.
[14]   Guendez R, Kallithraka S, Makris D P, Kefalas P. Determination of low molecular weight polyphenolic constituents in grape (Vitis vinifera sp1) seed extracts: Correlation with antiradical activity. Food Chemistry, 2005, 89: 1-9.
[15]   李凤英. 葡萄皮、籽多酚提取技术研究[D]. 北京: 中国农业大学, 2004.
Li F Y. Grape skins, seeds polyphenol research extraction technology[D]. Beijing: China Agricultural University, 2004. (in Chinese)
[16]   田淑芬. 中国葡萄产业态势分析. 中外葡萄与葡萄酒. 2009(1): 64-66.
Tian S F. Chinese wine industry trend analysis. Foreign Grapes and Wine, 2009(1): 64-66. (in Chinese)
[17]   Rodriguez-Delgado M A, Gonzlea-Hemandez G, Conde-Gonzalez J E. Principal component analysis of the polyphenol content in young red wines. Food Chemistry, 2002, 78: 523-532.
[18]   Proestos C, Bakogiannis A, Psarianos C, Athanasios A, Kanellaki M, Komaitis M. High performance liquid chromatography analysis of phenolic substances in Greek wines. Food Control, 2005, 16: 319-323.
[19]   Boselli E, Minardi M, Giomo A, Frega N. Phenolic composition and quality of white D.O.C. wines from Marche (Italy). Analytica Chimica Acta, 2006, 563: 93-100.
[20]   樊玺, 李记明. 不同种酿酒葡萄酚类物质特性研究, 中外葡萄与葡萄酒, 2000(4): 13-15.
Fan X, Li J M. Study on the phenolic characteristics of different vitis. Sino-Overseas Grapevine & Wine, 2000(4), 13-15. (in Chinese)
[21]   Bentez P, Castro R, Sanchez P J A. Influence of metallic content of fino sherry wine on its susceptibility to browning. Food Research International, 2002(35): 785-791.
[22]   Bakker J, Preston N W, Timberlake C F. The determination of anthocyanins in aging red wines: Comparison of HPLC and spectral methods. American Journal of Enology and Viticulture, 1986, 37: 21-26.
[23]   梁冬梅, 李记明, 林玉华. 分光光度计测葡萄酒的色度. 中外葡萄与葡萄酒, 2002(3): 9-13.
Liang D M, Li J M, Lin Y H. Spectrophotometer wine chromaticity. Sino-Overseas Grapevine & Wine, 2002(3): 9-13. (in Chinese)
[24]   房玉林, 孟江飞, 张昂, 张振文. 罐储时间对赤霞珠葡萄酒中酚类化合物及抗氧化活性的影响. 食品科学, 2011(11): 14-20.
Fang Y L, Meng J F, Zhang A, Zhang Z W. Effect of storage time on phenolic components and antioxidant activity of red wine. Food Science, 2011(11): 14-20. (in Chinese)
[25]   Hufnagel J C, Hofmann T. Orosensory-directed identification of astringent mouthfeel and bitter-tasting compounds in red wine. Journal of Agricultural and Food Chemistry, 2008, 56: 1376-1386.
[26]   Elias O S, Alvaro P N, Remigio L S. Comparative study of the phenolic composition of seeds and skins from Carmé nè re and Cabernet Sauvignon grape varieties (Vitis vinifera L.) during ripening. Journal of Agricultural and Food Chemistry, 2010, 58: 3591-3599.
[27]   赵文杰, 薛冰, 胡明华. 葡萄皮渣中单宁的提取纯化及含量测定. 中国酿造, 2010, 221(8): 152-156.
Zhao W J. Xue B. Hu M H. Extracting and content grape tannin determination. Chinese Brewing, 2010, 221(8): 152-156. (in Chinese)
[28]   Anastasiadi M, Pratsinis H, Kletsas D. Bioactive non-coloured polyphenols content of grapes, wines and vinification by-products: Evaluation of the antioxidant activities of their extracts. Food Research International, 2010, 43(3): 805-813.
[29]   王晓宇. 葡萄酒抗氧化活性及其检测方法的研究[D]. 陕西西安: 西北农林科技大学, 2009.
Wang X Y. Oxidation activity and anti-detection methods wine[D]. Shaanxi, Xian: Northwest Agriculture and Forestry University, 2009. (in Chinese)
[30]   González-Neves G, Charamelo D, Balado J, Barreiro L, Bochicchio R, Gatto G, Gil G, Tessore A, Carbonneau A, Moutounet M. Phenolic potential of Tannat, Cabernet-Sauvignon and Merlot grapes and their correspondence with wine composition. Analytica Chimica Acta, 2004, 513: 191-196.
[31]   王秀芹, 张庆华, 战吉成, 燕国梁, 黄卫东. 产地与品种对葡萄酒中酚类物质含量的影响. 食品科学, 2009, 30(21): 113-118.  
Wang X Q, Zhang Q H, Zhan J C, Yan G L, Huang W D. Effects of grape varieties and geographical origins on contents of phenolic compounds in grape wine. Food Science, 2009, 30(21): 113-118. (in Chinese)
[32]   刘丽媛, 苑伟, 刘延琳. 红葡萄酒中花色苷辅助成色作用的研究进展. 中国农业科学, 2010, 43(12): 2518-2526.
Liu L Y, Yuan W, Liu Y L. Advances in research of red wine’s anthocyanin copigmentation. Scientia Agricultura Sinica, 2010, 43(12): 2518-2526. (in Chinese)
[33]   Sun B S, Pinto T, Leandro M C. Transfer of catechins and proanthoeyanidins from solid parts of the grape cluster into wine. American Journal of Enology and Viticulture, 1999, 50: 179-183.
[34]   Souquet J M, Cheynier V, Moutounet M. Les Proanthoeyanidines du raisin. Bulletin of International Organization of Vine and Wine, 2000, 73: 835-836.
[35]   Souquet J M, Labarbe B, Le Guerneve C. Phenolic composition of grape stems. Journal of Agricultural and Food Chemistry, 2000, 48: 1076-1080.
[36] Souquet J M, Cheynier V, Brossaud F. Polymeric proanthocyanidins from grape skins. Phytochemistry, 1996, 43: 509-512.
[1] ZHANG KeKun,CHEN KeQin,LI WanPing,QIAO HaoRong,ZHANG JunXia,LIU FengZhi,FANG YuLin,WANG HaiBo. Effects of Irrigation Amount on Berry Development and Aroma Components Accumulation of Shine Muscat Grape in Root-Restricted Cultivation [J]. Scientia Agricultura Sinica, 2023, 56(1): 129-143.
[2] LÜ XinNing,WANG Yue,JIA RunPu,WANG ShengNan,YAO YuXin. Effects of Melatonin Treatment on Quality of Stored Shine Muscat Grapes Under Different Storage Temperatures [J]. Scientia Agricultura Sinica, 2022, 55(7): 1411-1422.
[3] GUO ZeXi,SUN DaYun,QU JunJie,PAN FengYing,LIU LuLu,YIN Ling. The Role of Chalcone Synthase Gene in Grape Resistance to Gray Mold and Downy Mildew [J]. Scientia Agricultura Sinica, 2022, 55(6): 1139-1148.
[4] WANG HuiLing, YAN AiLing, SUN Lei, ZHANG GuoJun, WANG XiaoYue, REN JianCheng, XU HaiYing. eQTL Analysis of Key Monoterpene Biosynthesis Genes in Table Grape [J]. Scientia Agricultura Sinica, 2022, 55(5): 977-990.
[5] WANG Bo,QIN FuQiang,DENG FengYing,LUO HuiGe,CHEN XiangFei,CHENG Guo,BAI Yang,HUANG XiaoYun,HAN JiaYu,CAO XiongJun,BAI XianJin. Difference in Flavonoid Composition and Content Between Summer and Winter Grape Berries of Shine Muscat Under Two-Crop-a-Year Cultivation [J]. Scientia Agricultura Sinica, 2022, 55(22): 4473-4486.
[6] LIU Xin,ZHANG YaHong,YUAN Miao,DANG ShiZhuo,ZHOU Juan. Transcriptome Analysis During Flower Bud Differentiation of Red Globe Grape [J]. Scientia Agricultura Sinica, 2022, 55(20): 4020-4035.
[7] MA YuQuan,WANG XiaoLong,LI YuMei,WANG XiaoDi,LIU FengZhi,WANG HaiBo. Differences in Nutrient Absorption and Utilization of 87-1 Grape Variety Under Different Rootstock Facilities [J]. Scientia Agricultura Sinica, 2022, 55(19): 3822-3830.
[8] JI XiaoHao,LIU FengZhi,WANG BaoLiang,LIU PeiPei,WANG HaiBo. Genetic Variation of Alcohol Acyltransferase Encoding Gene in Grape [J]. Scientia Agricultura Sinica, 2022, 55(14): 2797-2811.
[9] YANG ShengDi,MENG XiangXuan,GUO DaLong,PEI MaoSong,LIU HaiNan,WEI TongLu,YU YiHe. Co-Expression Network and Transcriptional Regulation Analysis of Sulfur Dioxide-Induced Postharvest Abscission of Kyoho Grape [J]. Scientia Agricultura Sinica, 2022, 55(11): 2214-2226.
[10] HAN Xiao, YANG HangYu, CHEN WeiKai, WANG Jun, HE Fei. Effects of Different Rootstocks on Flavonoids of Vitis vinifera L. cv. Tannat Grape Fruits [J]. Scientia Agricultura Sinica, 2022, 55(10): 2013-2025.
[11] XU XianBin,GENG XiaoYue,LI Hui,SUN LiJuan,ZHENG Huan,TAO JianMin. Transcriptome Analysis of Genes Involved in ABA-Induced Anthocyanin Accumulation in Grape [J]. Scientia Agricultura Sinica, 2022, 55(1): 134-151.
[12] LIU Chuang,GAO Zhen,YAO YuXin,DU YuanPeng. Functional Identification of Grape Potassium Ion Transporter VviHKT1;7 Under Salt Stress [J]. Scientia Agricultura Sinica, 2021, 54(9): 1952-1963.
[13] XuXian XUAN,ZiLu SHENG,ZhenQiang XIE,YuQing HUANG,PeiJie GONG,Chuan ZHANG,Ting ZHENG,Chen WANG,JingGui FANG. Function Analysis of vvi-miR172s and Their Target Genes Response to Gibberellin Regulation of Grape Berry Development [J]. Scientia Agricultura Sinica, 2021, 54(6): 1199-1217.
[14] PeiPei ZHU,YiJia LUO,Wen XIANG,MingLei ZHANG,JianXia ZHANG. Rescue and Molecular Marker Assisted-Selection of the Cold-Resistant Seedless Grape Hybrid Embryo [J]. Scientia Agricultura Sinica, 2021, 54(6): 1218-1228.
[15] ZHANG Lu,ZONG YaQi,XU WeiHua,HAN Lei,SUN ZhenYu,CHEN ZhaoHui,CHEN SongLi,ZHANG Kai,CHENG JieShan,TANG MeiLing,ZHANG HongXia,SONG ZhiZhong. Identification, Cloning, and Expression Characteristics Analysis of Fe-S Cluster Assembly Genes in Grape [J]. Scientia Agricultura Sinica, 2021, 54(23): 5068-5082.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!