不同秸秆生物炭对红壤性水稻土养分及微生物群落结构的影响

doi:10.3864/j.issn.0578-1752.2015.07.11

Abstract

Abstract: 【Objective】The various effects of different straw biochar on nutrient content and microbial community structure were studied in order to provide information for soil amelioration and proper management of straw residue.【Method】Through a 135-day laboratory incubation experiment and used a red paddy soil that originated from the Quaternary, the influences of rice and corn straw biochar that pyrolyzed at 300℃, 400℃ and 500℃ on soil pH, organic carbon, nutrient content, microbial biomass carbon and profile of microbial PLFAs community structure were investigated. The experiment consisted of seven treatments: control soil (CK), soil amended with 300℃ (RB300), 400℃ (RB400) and 500℃ (RB500) rice straw biochar, soil incorporated with 300℃ (CB300), 400℃ (CB400) and 500℃ (CB500) corn straw biochar. 【Result】 Feedstock type and pyrolysis temperature had a significant influence on the nutrient contents and chemical properties of biochar products. Compared with control, the two straw biochar amendments increased pH value by 0.16 unit and enhanced the contents of soil organic carbon, available P and available K by 26.1%, 20.6% and 281.8%, respectively. Under the same pyrolysis temperature, the application of rice straw biochar mainly promoted the level of available K while corn straw biochar improved the content of available P. Application of 300℃ straw biochar had no significant effect on soil available and mineral N contents. Compared with the control, soils amended with RB500 and CB500 were, respectively, 10.4% and 8.1% less in available N, while significantly increased by 63.6% and 100.7% in NO₃^--N concentration. Although the concentrations of microbial biomass carbon and total phospholipid fatty acids for soils amended with straw biochar were 63.4% and 47.5% higher than control soil, there was no significant difference between the control soil and soils with 300℃ straw biochar. Both the two types of biochar enhanced the contents of G-, G+, fungi and actinobacteria and shown as 300℃＜400℃＜500℃. Results of PCA indicated that rice straw biochar amendment had more effect on the structure of soil microbial community than corn straw biochar. The microbial community compositions of three rice straw biochar were separated from each other while no distinctive recognized among the three corn biochar. Results of CCA suggested that straw biochars can affect the composition of microbial community through altering soil chemical and nutrient properties, as soil available P, soil organic carbon and available had significant correlation with the distribution of soil microbial community. 【Conclusion】 Both the two straw biochars could ameliorate the acidity and nutrient content of red paddy soil, and enhance the level of soil microbial biomass. Soil microbial community structure had been affected in the presence of straw biochars and rice straw biochar had more effective influence than corn straw biochars.

Key words: straw biochar, soil nutrient, microbial biomass carbon, phospholipid fatty acids, canonical correspondence analysis

LI Ming, LI Zhong-pei, LIU Ming, JIANG Chun-yu, WU Meng. Effects of Different Straw Biochar on Nutrient and Microbial Community Structure of a Red Paddy Soil[J].Scientia Agricultura Sinica, 2015, 48(7): 1361-1369.

References

[1] Wang Y J, Bi Y Y, Gao C Y. The assessment and utilization of straw resources in china. Agricultural Sciences in China, 2010, 9(12): 1807-1815.

[2] Yuan J H, Xu R K, Qian W, Wang R H. Comparison of the ameliorating effects on an acidic ultisol between four crop straws and their biochars. Journal of Soils and Sediments, 2011, 11(5): 741-750.

[3] Hua L, Lu Z Q, Ma H R, Jin S S. Effect of biochar on carbon dioxide release, organic carbon accumulation, and aggregation of soil. Environmental Progress & Sustainable Energy, 2014, 33(3): 941-946.

[4] 张斌, 刘晓雨, 潘根兴, 郑聚锋, 池忠志, 李恋卿, 张旭辉, 郑金伟. 施用生物质炭后稻田土壤性质、水稻产量和痕量温室气体排放的变化. 中国农业科学, 2012, 45(23): 4844-4853.

Zhang B, Liu X Y, Pan G X, Zheng J F, Chi Z Z, Li L Q, Zhang X H, Zheng J W. Changes in soil properties, yield and trace gas emission from a paddy after biochar amendment in two consecutive rice growing cycles. Scientia Agricultura Sinica, 2012, 45(23): 4844-4853. (in Chinese)

[5] Lehmann J, Gaunt J, Rondon M. Bio-char sequestration in terrestrial ecosystems-a review. Mitigation and Adaptation Strategies for Global Change, 2006, 11: 395-419.

[6] 陈温福, 张伟明, 孟军. 农用生物炭研究进展与前景. 中国农业科学, 2013, 46(16): 3324-3333.

Chen W F, Zhang W M, Meng J. Advances and prospects in research of biochar utilization in agriculture. Scientia Agricultura Sinica, 2013, 46(16): 3324-3333. (in Chinese)

[7] Woolf D, Amonette J E, Street-Perrott F A, Lehmann J, Joseph S. Sustainable biochar to mitigate global climate change. Nature communications, 2010, 1: 56.

[8] Xie Z, Xu Y, Liu G, Qi L, Zhu J G, Tu C, James E. Amonette J E, Cadisch G, Yong J W H, Hu S J. Impact of biochar application on nitrogen nutrition of rice, greenhouse-gas emissions and soil organic carbon dynamics in two paddy soils of China. Plant and soil, 2013, 370(1/2): 527-540.

[9] Zhao X, Wang S Q, Xing G X. Nitrification, acidification, and nitrogen leaching from subtropical cropland soils as affected by rice straw-based biochar: laboratory incubation and column leaching studies. Journal of Soils and Sediments. 2014, 14: 471-482.

[10] Liang B, Lehmann J, Solomon D, Kinyangi J, Grossman J, O'Neill B, Skjemstad J O, Thies J, Luizao F J, Petersen J, Neves E G. Black carbon increases cation exchange capacity in soils. Soil Science Society of America Journal, 2006, 70(5): 1719-1730.

[11] 周桂玉, 窦森, 刘世杰. 生物质炭结构性质及其对土壤有效养分和腐殖质组成的影响. 农业环境科学学报, 2011, 10: 2075-2080.

Zhou G Y, Dou S, Liu S J. The Structural characteristics of biochar and its effects on soil available nutrients and humus composition. Journal of Agro-Environment Science, 2011, 10: 2075-2080. (in Chinese)

[12] 张晗芝, 黄云, 刘钢, 许燕萍, 刘金山, 卑其诚, 蔺兴武, 朱建国, 谢祖彬. 生物炭对玉米苗期生长、养分吸收及土壤化学性状的影响. 生态环境学报, 2010, 11: 2713-2717.

Zhang H Z, Huang Y, Liu G, Xu Y P, Liu J S, Bei Q C, Lin X W, Zhu J G, Xie Z B. Effects of biochar on corn growth, nutrient uptake and soil chemical properties in seeding stage. Ecology and Environmental Sciences, 2010, 11: 2713-2717. (in Chinese)

[13] Yuan J H, Xu R K, Zhang H. The forms of alkalis in the biochar produced from crop residues at different temperatures. Bioresource Technology, 2011, 102(3): 3488-3497.

[14] Fu P, Hu S, Xiang J, Sun L S, Li P S, Zhang J Y, Zheng C G. Pyrolysis of maize stalk on the characterization of chars formed under different devolatilization conditions. Energy & Fuels, 2009, 23: 4605-4611.

[15] Goldberg. Black Carbon in the Environment: Properties and Distribution. New York: John Wiley, 1985.

[16] Lehmann J, Rillig M C, Thies J, Masiello C A, Hockaday W C, Crowley D. Biochar effects on soil biota-a review. Soil Biology & Biochemistry, 2011, 43 (9): 1812-1836.

[17] 陈心想, 耿增超, 王森, 赵宏飞. 施用生物炭后塿土土壤微生物及酶活性变化特征. 农业环境科学学报, 2014, 33(4): 751-758.

Chen X X, Geng Z C, Wang S, Zhao H F. Effects of biochar amendment on microbial biomass and enzyme activities in loess soil. Journal of Agro-Environment Science, 2014, 33(4): 751-758. (in Chinese)

[18] Kolb S E, Fermanich K J, Dornbush M E. Effect of charcoal quantity on microbial biomass and activity in temperate soils. Soil Science Society of America Journal, 2009, 73(4): 1173-1181.

[19] Dempster D N, Gleeson D B, Solaiman Z M, Jones D L, Murphy D V. Decreased soil microbial biomass and nitrogen mineralisation with eucalyptus biochar addition to a coarse textured soil. Plant and Soil, 2012, 354 (1/2): 311-324.

[20] Steinbeiss S, Gleixner G, Antonietti M. Effect of biochar amendment on soil carbon balance and soil microbial activity. Soil Biology and Biochemistry, 2009, 41(6): 1301-1310.

[21] Muhammad N, Dai Z M, Xiao K C, Meng J, Brookes P C, Liu X M, Wang H Z, Wu J J, Xu J M. Changes in microbial community structure due to biochars generated from different feedstocks and their relationships with soil chemical properties. Geoderma, 2014, 226: 270-278.

[22] Masiello C A, Chen Y, Gao X D, Liu S, Cheng H Y, Bennett M R, Rudgers J A, Wagner D S, Zygourakis K, Silberg J J. Biochar and microbial signaling: Production conditions determine effects on microbial communication. Environmental Science & Technology, 2013, 47(20): 11496-11503.

[23] Ameloot N, Sleutel S, Das K C, Kanagaratnam J, De Neve S. Biochar amendment to soils with contrasting organic matter level: effects on N mineralization and biological soil properties. Global Change Biology Bioenergy, 2013, doi: 10.1111/gcbb.12119.

[24] 鲁如坤. 土壤农业化学分析方法. 北京: 中国农业科技出版社, 1999.

Lu R K. Analytical Methods of Soil Agricultural Chemistry. Beijing: Chinese Agricultural Science and Technology Press, 1999. (in Chinese)

[25] Boehm H P. Surface oxides on carbon and their analysis: a critical assessment. Carbon, 2002, 40(2): 145-149.

[26] Bligh E G, Dyer W J. A Rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology, 1959, 37: 911-917.

[27] Van Zwieten L, Kimber S, Morris S, Chan KY, Downie A, Rust J, Joseph S，Cowie A. Effects of biochar from slow pyrolysis of papermill waste on agronomic performance and soil fertility. Plant and Soil, 2010, 327: 235-246.

[28] Novak J M, Busscher W J, Laird D L, Ahmedna M, Watts D W, Niandou M A. Impact of biochar amendment on fertility of a southeastern coastal plain soil. Soil Science, 2009, 174(2): 105-112.

[29] Van Zwieten L, Kimber S, Downie A, Morris S, Petty S, Rust J, Chan K Y. A glasshouse study on the interaction of low mineral ash biochar with nitrogen in a sandy soil. Soil Research, 2010, 48: 569-576.

[30] Gaskin J W, Speir R A, Harris K, Das K C, Lee R D, Morris L A, Fisher D S. Effect of peanut hull and pine chip biochar on soil nutrients, corn nutrient status, and yield. Agronomy Journal, 2010, 102: 623-633.

[31] 张祥, 王典, 姜存仓, 朱盼, 雷晶, 彭抒昂. 生物炭对我国南方红壤和黄棕壤理化性质的影响. 中国生态农业学报, 2013, 21(8): 979-984.

Zhang X, Wang D, Jiang C C, Zhu P, Lei J, Peng S A. Effect of biochar on physicochemical properties of red and yellow brown soils in the South China Region. Chinese Journal of Eco-Agriculture, 2013, 21(8): 979-984. (in Chinese)

[32] Schomberg H H, Gaskin J W, Harris K, Das K C, Novak J M, Busscher W J, Watts D W, Woodroof R H, Lima I M, Ahmedna M, Rehrah D, Xing B S. Influence of biochar on nitrogen fractions in a coastal plain soil. Journal of Environmental Quality, 2012, 41(4): 1087-1095.

[33] Maestrini B, Herrmann A M, Nannipieri P, Schmidt M W I, Abiven S. Ryegrass-derived pyrogenic organic matter changes organic carbon and nitrogen mineralization in a temperate forest soil. Soil Biology & Biochemistry, 2014, 69: 291-301.

[34] Luo Y, Durenkamp M, De Nobili M, Lin Q, Devonshire B J, Brookes P C. Microbial biomass growth, following incorporation of biochars produced at 350 ℃ or 700 ℃, in a silty-clay loam soil of high and low pH. Soil Biology & Biochemistry, 2013, 57: 513-523.

[35] Farrell M, Kuhn T K, Macdonald L M, Maddern T M, Murphy D V, Hall P A, Singh B P, Baumann K, Krull E S, Baldock J A. Microbial utilisation of biochar-derived carbon. Science of the Total Environment, 2013, 465: 288-297.

[36] 陈伟, 周波, 束怀瑞. 生物炭和有机肥处理对平邑甜茶根系和土壤微生物群落功能多样性的影响. 中国农业科学, 2013, 46(18): 3850-3856.

Chen W, Zhou B, Shu H R. Effects of organic fertilizer and biochar on root system and microbial functional diversity of Malus hupehensis Rehd. Scientia Agricultura Sinica. 2013, 46(18): 3850-3856. (in Chinese)

[37] Lü M R, Li Z P, Che Y P, Han F X, Liu M. Soil organic c, nutrients, microbial biomass, and grain yield of rice (oryza sativa L.) after 18 years of fertilizer application to an infertile paddy soil. Biology and Fertility of Soils, 2011, 47(7): 777-783.

[38] Kuzyakov Y, Subbotina I, Chen H Q, Bogomolova I, Xu X L. Black carbon decomposition and incorporation into soil microbial biomass estimated by C-14 labeling. Soil Biology & Biochemistry, 2009, 41(2): 210-219.

Related Articles 15

[1]	LI YongJuan, ZHANG YueTong, WANG YiBo, ZHAO ChangJiang, SONG Jie, CHEN XueLi, YAO Qin. Effects of Biochar Application on the Abundance and Community Composition of Nitrogen-Fixing Microbial nifH Gene in Soybean Rotation and Continuous Cropping Systems [J]. Scientia Agricultura Sinica, 2026, 59(6): 1272-1285.
[2]	LUO YiNuo, LI YanFei, LI WenHu, ZHANG SiQi, MU WenYan, HUANG Ning, SUN RuiQing, DING YuLan, SHE WenTing, SONG WenBin, LI XiaoHan, SHI Mei, WANG ZhaoHui. Iron Concentrations in Grain and Its Different Parts of Newly Developed Wheat Varieties (Lines) in China and Influencing Factors [J]. Scientia Agricultura Sinica, 2025, 58(3): 416-430.
[3]	ZHANG Feng, XU JunFeng, GAO ZhiYuan, DANG HaiYan, GUO RongBo, SHE WenTing, LI WenHu, LIU JinShan, WANG ZhaoHui. Optimizing Wheat Nitrogen and Phosphorus Fertilizer Rates Based on Apparent Nitrogen and Phosphorus Balance in a Long-Term Location Fixed Field Experiment [J]. Scientia Agricultura Sinica, 2025, 58(22): 4688-4702.
[4]	ZHANG SiJia, YANG Jie, ZHAO Shuai, LI LiWei, WANG GuiYan. The Impact of Diversified Crops and Wheat-Maize Rotations on Soil Quality in the North China Plain [J]. Scientia Agricultura Sinica, 2025, 58(2): 238-251.
[5]	WU Yong, WEN Xue, WANG TianShu, HUANG YanYan, MENG YiLi, JIANG HongYu, BI LiDong, WU HuiJun, YAO ShuiHong. The Influence of Topographic Factors and Ridge Tillage Methods on Soil Nutrients and Fertility Index of Sloping Arable Land in the Black Soil Region [J]. Scientia Agricultura Sinica, 2025, 58(18): 3676-3689.
[6]	GUO Lei, ZHANG BinBin, SHEN ZhiJun, YAN Juan, XU JianLan, CAI ZhiXiang, YU MingLiang, WANG FaLin, SONG HongFeng. The Release Characteristics of Medium and Trace Elements and Their Effects on Soil Available Nutrients after the Continuous Return of Green Manure in Peach Orchards [J]. Scientia Agricultura Sinica, 2025, 58(12): 2411-2426.
[7]	LUO YuHong, HUANG YuShu, ZHU Na, LI Le, CHENG YanBin, LIU JiaHui, ZHANG JingMin, BAO YuFan, XU Nuo, YAN YuChun. Effects of Cultivation and Cropland Afforestation on Soil Particle- Size Distribution and Soil Nutrients in the Typical Steppe of Xilingol League [J]. Scientia Agricultura Sinica, 2024, 57(24): 4919-4932.
[8]	LI HaiPeng, DU WuYan, WU HanQian, ZHANG Jie, MENG HuiSheng, HONG JianPing, XU MingGang, HAO XianJun, GAO WenJun. Different Manures Affect Soil Nutrients and Bacterial Community Structure in Mining Reclamation Area [J]. Scientia Agricultura Sinica, 2024, 57(16): 3207-3219.
[9]	SU HaiLan, ZHU YanMing, CHEN Hong, NIU YuQing, ZHENG MeiXia, ZHU YuJing. Effects of Different Intercropping Methods on Mesona Chinesis Quality and Its Rhizosphere Soil Characteristic [J]. Scientia Agricultura Sinica, 2024, 57(14): 2755-2770.
[10]	WANG QingYang, CAO DianYun, WANG Di, ZHAN ZengYi, HE WanYing, SUN Qiang, CHEN WenFu, LAN Yu. Effects of Long-Term Application of Biochar on Nutrients, Fractions of Humic in Brown Soil [J]. Scientia Agricultura Sinica, 2024, 57(13): 2612-2622.
[11]	ZHAO WeiSong, GUO QingGang, CUI NaQi, LU XiuYun, LI SheZeng, MA Ping. Effects of Exogenous Addition of L-Proline on the Occurrence of Cotton Verticillium Wilt and Its Soil Microbial Community in Rhizosphere [J]. Scientia Agricultura Sinica, 2024, 57(11): 2143-2160.
[12]	BU MingNa, YANG XiWen, TENG ZhengKai, HU NaiYue, ZHANG Shuo, WANG ChunYan, YANG Jian, LIANG WenXian, MA WenQi, HE DeXian, ZHOU SuMei. Effects of Layered Fertilization Under Different Irrigation Conditions on Vertical Distribution of Soil Nutrients and Root Growth and Function of Wheat [J]. Scientia Agricultura Sinica, 2024, 57(11): 2125-2142.
[13]	DONG Xiu, ZHANG Yan, MUNYAMPIRWA Tito, TAO HaiNing, SHEN YuYing. Effects of Long-Term Conservation Tillage on Soil Carbon Content and Invertase Activity in Dry Farmland on the Loess Plateau [J]. Scientia Agricultura Sinica, 2023, 56(5): 907-919.
[14]	ZHU ChangWei,MENG WeiWei,SHI Ke,NIU RunZhi,JIANG GuiYing,SHEN FengMin,LIU Fang,LIU ShiLiang. The Characteristics of Soil Nutrients and Soil Enzyme Activities During Wheat Growth Stage Under Different Tillage Patterns [J]. Scientia Agricultura Sinica, 2022, 55(21): 4237-4251.
[15]	GUO YingXin,CHEN YongLiang,MIAO Qi,FAN ZhiYong,SUN JunWei,CUI ZhenLing,LI JunYing. Spatial-Temporal Variability of Soil Nutrients and Assessment of Soil Fertility in Erhai Lake Basin [J]. Scientia Agricultura Sinica, 2022, 55(10): 1987-1999.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

Effects of Different Straw Biochar on Nutrient and Microbial Community Structure of a Red Paddy Soil

RichHTML

PDF

Cited