小麦粒重基因TaCwi-A1功能标记CWI22、CWI21的验证及应用

doi:10.3864/j.issn.0578-1752.2014.13.019

Abstract

Abstract: 【Objective】In order to provide some information for molecular marker assisted selection, the function markers CWI22 and CWI21 of TaCwi-A1 gene related to wheat kernel weight were validated. The allelic frequencies at TaCwi-A1 locus were detected by those markers in Xinjiang wheat germplasm. 【Method】The 110 Xinjiang winter wheat varieties were genotyped with CWI22 and CWI21, and the difference in thousand kernel weight (TKW) among varieties with TaCwi-A1a and TaCwi-A1b were analyzed. The allelic variations at TaCwi-A1 locus were detected by CWI22 and CWI21 in a total of 1 241 Xinjiang wheat germplasms. 【Result】In the 110 Xinjiang winter wheat varieties, a 402-bp fragment could be amplified by CWI22 in 46 varieties, which means they had genotype of TaCwi-A1a, a 404-bp fragment could be amplified by CWI21 in 64 varieties, which means they had genotype of TaCwi-A1b. And the TKW of varieties with TaCwi-A1a (43.5 g) was significantly higher than that with TaCwi-A1b (40.9 g) (P＜0.05). In the 1 241 Xinjiang wheat germplasms, the frequencies of TaCwi-A1a and TaCwi-A1b were 62.6% and 37.4% in all germplasms, 63.0% and 37.0% in winter wheats varieties, 61.7% and 38.3% in spring wheat varieties, respectively. In different types of varieties of both winter and spring wheat germplasm, the frequency of TaCwi-A1a was in order of exotic varieties (lines)＞domestic varieties (lines)＞breeding varieties (lines)＞released varieties＞landraces. In Xinjiang released varieties, the frequencies of TaCwi-A1a and TaCwi-A1b were 40.0% and 60.0% in winter wheats, 68.6% and 31.4% in spring wheats, respectively. In three stages of before 1990, 1991 to 2000, and after 2001, the frequencies of TaCwi-A1a and TaCwi-A1b were 11.1% and 88.9%, 50.0% and 50.0%, 69.2% and 30.8%, respectively. 【Conclusion】The markers CWI22 and CWI21 of TaCwi-A1 gene can efficiently distinguish the differences of wheat TKW, which can be used for marker assisted selection of kernel weight (KW). In Xinjiang wheat germplasm, TaCwi-A1a gene has a high frequency. And the frequency of TaCwi-A1a gene in winter wheat germplasm is a little higher than spring wheat germplasm, introduced varieties (lines) is higher than breeding varieties (lines), breeding varieties (lines) is higher than landraces. In both landraces and breeding varieties (lines), the frequencies of TaCwi-A1a gene in spring wheat varieties are higher than winter wheat varieties, which means that the breeding selection of KW in winter and spring wheat are different in Xinjiang, but there had high selection pressure in all, which makes the frequency of TaCwi-A1a gene in released varieties increase.

Key words: Xinjiang wheat , germplasm , kernel weight , CWI , function marker

XIANG Ji-Shan-1, 2 , MU Pei-Yuan-1, 2 , SANG Wei-1, 2 , NIE Ying-Bin-1, XU Hong-Jun-1, ZHUANG Li-2, 3 , CUI Feng-Juan-1, 2 , HAN Xin-Nian-1, ZOU Bo-1. Validation and Application of Function Markers CWI22 and CWI21 of TaCwi-A1 Gene Related to Wheat Kernel Weight[J].Scientia Agricultura Sinica, 2014, 47(13): 2671-2679.

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Validation and Application of Function Markers CWI22 and CWI21 of TaCwi-A1 Gene Related to Wheat Kernel Weight

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