Scientia Agricultura Sinica ›› 2014, Vol. 47 ›› Issue (9): 1836-1846.doi: 10.3864/j.issn.0578-1752.2014.09.019

• RESEARCH NOTES • Previous Articles     Next Articles

Genetic Diversity of Helicoverpa assulta (Lepidoptera: Noctuidae) in Guizhou Province

 SHI  Pei-Qiong-1, YANG  Mao-Fa-1, 吕Zhao-Yun-1 , LI  Shang-Wei-1, LIAO  Qi-Rong-1, SHANG  Sheng-Hua-2, XU  Jin-1, WU  Yi-Bei-1   

  1. 1、Institute of Entomology, Guizhou University/Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Guiyang 550025;
    2、Guizhou Tobacco Science Research Institute, Guiyang 550081
  • Received:2013-10-15 Online:2014-05-01 Published:2013-12-02

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Abstract: 【Objective】 The objectives of the study are to explore genetic differentiation of oriental tobacco budworm (Helicoverpa assulta) among different geographic populations in Guizhou, uncover the differential law and mechanism, and to provide a scientific basis for the pest monitoring and integrated control. 【Method】 PCR amplifications were performed by 6 pairs of SSR primers that were screened out from 43 pairs of primers from closely-related species, using DNA from 30 H. assulta geographic populations in Guizhou as template. The PCR products were subjected to 8% non-denaturing polyacrylamide gelelectrophoresis and visualized by silver staining. Genetic diversity was analyzed by PopGene Version 1.32, and the UPGMA dendrogram was constructed by using MEGA 5.0 based on the Nei’s genetic distance. The correlation between genetic and geographic distances as well as between genetic identity and elevation were analyzed by using Mantel. 【Result】 The observed and effective number of alleles ranged from 3 to 8 with an average of 5 and from 1.4498 to 2.2219 with an average of 1.8594, respectively. Shannon’s information index ranged from 0.5310 to 1.0609 with an average of 0.8423. The observed (Ho) and expected heterozygosity (He) varied from 0.0260 to 0.2672 with a mean of 0.1239 and from 0.3123 to 0.5520 with a mean of 0.4539, respectively. The He was higher than the Ho, suggesting that all populations were mainly in homozygous condition. FIS changed from 0.0798 to 0.7906 with a mean of 0.2801; FIT changed from 0.4842 to 0.9731 with a mean of 0.7809. Both FIS and FIT were positive, indicating that there existed inbreeding in the all populations of H. assulta from tobacco-growing areas of Guizhou. FST ranged from 0.3897 to 0.9256 and gene flow (Nm) was less than 1, which reflected high genetic differentiation and a low level of Nm among all the populations. Genetic distance was in a range of 0.0068-2.5193 and genetic identity was in a range of 0.1051-0.9933. The minimum genetic distance (0.0068) and the maximum genetic identity (0.9933) were observed between Songtao and Yinjiang populations, whereas the maximum genetic distance (2.5193) and the minimum genetic identity (0.1051) were observed between Daozhen and Hezhang populations. The UPGMA phylogenetic analysis showed that the 30 geographic populations in Guizhou were clustered into three groups. The clustering had no significant correlation with the geographic distribution and only in a few populations the genetic differentiation had relationship with geographic distance. Mantel tests demonstrated that there was no significant correlation between genetic and geographic distances as well as between genetic identity and elevation. 【Conclusion】 There exists a high level of genetic diversity among different geographic populations of H. assulta in Guizhou. All the populations are highly differentiated and the genetic differentiation primarily occurred between populations. Geographic isolation has no significant effect on the genetic differentiation.

Key words: Guizhou , Helicoverpa assulta , microsatellite marker , genetic diversity , genetic differentiation

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