Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (22): 4791-4799.doi: 10.3864/j.issn.0578-1752.2013.22.017

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Polymorphism of the 60149273th Loci on X Chromosome Among Fat Tail and Thin Tail Breeds and Its Gene Mapping

 GAN  Shang-Quan, SHEN  Min, LI  Huan, LIANG  Yao-Wei, YANG  Jing-Quan, GAO  Lei, LIU  Shou-Ren, WANG  Xin-Hua   

  1. Institute of Animal Husbandry and Veterinary, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, Xinjiang
  • Received:2013-06-24 Online:2013-11-15 Published:2013-07-31

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Abstract: 【Objective】 The objective of this paper is to study the polymorphism of the SNP located at the position of 60149247 on X chromosome between fat tail (rump) and thin tail breeds, and to provide the basic data for analysis of the mechanism of tail fat deposition in sheep.【Method】 Firstly, PCR-SSCP genotyping method was used to analyze genotypes among different tail types of sheep breeds, and definite allelic genes were cleared by PCR-direct sequencing, and the chi-square test was employed to detect the balance of genotype distribution in different breeds. Secondly, the SNP was mapped on sheep androgen receptor (AR) and the whole CDS sequences of AR were obtained by comparative genome and silicon cloning methods. Taking cDNA of Altay tail fat as DNA template, RT-PCR method was used to clone sheep AR spinning six exons, and sequence homology and phylogenetic tree were analyzed by bioinformatics methods. 【Result】The results of PCR-SSCP showed that there is no polymorphism in fat-tail sheep breeds (Hu sheep and Altay sheep), and the genotypes of the two breeds are all GG genotype. And there are three genotypes (GG, GA and AA) in thin-tail sheep populations (Chinese Merino sheep and Suffolk sheep), and the frequency of GG genotype dropped to 10% and 21%, respectively. The whole CDS sequences of sheep AR were cloned by silicon cloning, and sheep AR containing the fragment from the 3rd exon to 8th exon of AR was successfully cloned in total RNA of Altay tail fat for the first time, and the sequences are completely the same to the sequences predicted by silicon cloning. The sequence alignment also showed that AR is highly conserved in mammalian animals, and the homology was up to 90% among different mammalian animals, and the phylogenetic tree analysis showed that sheep is relatively close to bovine, pigs and dolphin. 【Conclusion】This study was the first to identify a novel SNP in the 3rd intron of the sheep AR associated with the trait of fat tail, and it hinted that the SNP will be used as a practical application of molecular markers in low-fat sheep breeding. At the same time, AR will also serve as an important candidate gene in studying tail fat deposition in Sheep.

Key words: fat tail trait , Altay sheep , polymorphism , genotype , marker gene

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