家蚕安全转基因技术研究现状与展望

doi:10.3864/j.issn.0578-1752.2018.02.015

Abstract

Abstract: Transgenic technology is an important tool for gene function analysis and genetic improvement of biological variety.At present, the safety problems of GMOs mainly focus on the safety of genetic manipulation techniques and genetically modified products. In recent years, the researches on the safety of GMOs werefocused on evaluating the security level of genetically modified crops, aquatic animals and poultry, livestock and other large animals in medicine, agriculture, and food industry, but the reports of the safety of genetic manipulation techniquesare still rare in the agricultural insects which have important scientific and economic value, and the Ministry of Agriculture has not yet set a precedent for the safety assessment of transgenic insects. The silkworm (Bombyx mori) is an important mode lepidopteran and economic insect for agriculture. In-depth research on the safety of transgenic technologies of B. mori, which has important value and significance for promoting the development of basic genetics and silk industry. Since the first report of the germline transformation of the B. mori using a piggyBac transposon-derived vector in 2000, transgenic technology has been widely used in the basic research of gene function identification and the applied research of creating new varieties with specific gene differences in B. mori. But the safety problems of transgenic B. mori have become the key problems that hindering the practical application of transgenic B. mori. Therefore, doing research on the safety of transgenic technologies of B. mori is of great significance to promote the safety assessment and industrialization of transgenic B. mori. This paper summarizes the establishment and research status of the conditional gene targeting-based safety of transgenic technologies of B. mori, while the development trends and application prospect of these technologies in B. mori are also discussed, as well as provide references for establishing and perfecting the safety of transgenic technologies in other transgenic animals, especially the agriculture insect species.

Key words: silkworm(Bombyx mori), biosafety, safe transgenic technology, site-specific recombination, marker gene deletion, target genome editing and integration

LONG DingPei, HAO ZhanZhang, XIANG ZhongHuai, ZHAO AiChun. Current Status of Transgenic Technologies for Safety Consideration in Silkworm (Bombyx mori) and Future Perspectives[J].Scientia Agricultura Sinica, 2018, 51(2): 363-373.

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