Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (19): 3955-3964.doi: 10.3864/j.issn.0578-1752.2013.19.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Characterization of the Regulatory Gene hrd1(t) Involved in Anthocyanin Biosynthesis

 LIU  Yong-Zhu, LUO  Wen-Long, HUANG  Cui-Hong, CHEN  Li-Kai, WANG  Hui, CHEN  Zhi-Qiang, GUO  Tao   

  1. South China Agricultural University/National Engineering Research Center of Plant Space Breeding, Guangzhou 510642
  • Received:2013-03-25 Online:2013-10-01 Published:2013-06-17

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Abstract: 【Objective】 The objective of this study is to conduct genetic analysis and gene mapping for the purple leaf character of rice germplasm hrd1 (Oryza sativa L. ssp. indica). 【Method】 The regulatory gene hrd1, is a rice germplasm with purple leaf which originated from the hybrids of two green leaf accessions Texianzhan 13 and 02428. Major agronomic traits of hrd1 was firstly investigated, and the segregation ratio of purple and green leaf characters was assayed by using the F1, F2 and BC1F1 populations derived from the crosses of hrd1 and 02428. Fine mapping for the gene contributed to purple leaf character was further conducted by using F2 population, and the candidate gene was identified. 【Result】 The purple character initiates on leaf apex of hrd1 at the 2nd leaf stage and gradually spreads to most parts of the whole plant. At the ripening stage, the agronomic trait of hrd1 was significantly different from the control cultivar (Texianzhan 13). Chlorophyll content was equal between hrd1 and the control, while the anthocyanin content in hrd1 was much higher than that in control. A recessive gene named hrd1(t) controls the purple leaf character in hrd1 according to genetic analysis, and it was mapped to a 32.5 kb region on chromosome 4 between InDel markers HRD10 and HRD21. By sequencing for genes within the mapped region, a single base mutation (A to C) was found in the 3rd exon of LOC_Os04g50660 (encoding a WD and G-beta repeats containing protein), which leads to the predicted amino acid at position 196 converts from lysine in control to threonine in hrd1. 【Conclusion】hrd1(t) encodes a WD40-class transcription factor and may be involved in a regulatory mechanism of pigments.

Key words: rice , purple leaf trait , anthocyanin , WD40 regulatory gene

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