Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (17): 3721-3728.doi: 10.3864/j.issn.0578-1752.2013.17.022

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Effects of Mating Flight on sRNAs Expression in Sexual Matured Virgin Queens (Apis cerana cerana)

 WU  Xiao-Bo, WANG  Zi-Long, SHI  Yuan-Yuan, ZHANG  Fei, ZENG  Zhi-Jiang   

  1. Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045
  • Received:2013-02-06 Online:2013-09-01 Published:2013-04-11

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Abstract: 【Objective】The objective of this study is to analyze the influence of flight behaviour on sRNAs expression in matured virgin queens (Apis cerana cerana). 【Method】The sexual matured virgin queens of A. cerana cerana were used as flying group that allow to fly in a certain area and the other group was not. The sRNAs expression differences between the two groups were analyzed by using a high-throughput sequencing method. 【Result】Both of the flying matured virgin queens and non-flying counterparts had a complicated sRNAs population, and that the length of sRNAs varied, 22 nt and 27-29 nt being the predominant length, while the frequence percent of the same RNA was different in the two samples. Combining deep sequencing and bioinformatics analysis, the percent of the common sequences in total sRNAs was 92.79%, but the number of unique sRNAs in the flying queens was more than that in non-flying queens. Matching to known miRNA alignment, a total of 25 known miRNAs were significantly different between them, with 1 up-regulated and 24 down-regulated in the flying matured virgin queens while there were 11 target genes of 19 known miRNA which were expressed differentially between them.【Conclusion】There are a large number of sRNAs changes during the flight process of matured virgin queens which may play an important role in adjusting physiology for mating successfully of virgin queens.

Key words: Apis cerana cerana , queen bee , flight , miRNA

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