Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (13): 2709-2716.doi: 10.3864/j.issn.0578-1752.2013.13.009

• SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT • Previous Articles     Next Articles

Effect of Soil pH on Soil Microbial Carbon Phosphorus Ratio

 LI  Chun-Yue, WANG  Yi, PhilipBrookes , DANG  Ting-Hui, WANG  Wan-Zhong   

  1. 1.Northwest Land and Resources Research Center, Shaanxi Normal University, Xi’an 710062, China
    2.Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK
    3.Institute of Soil and Water Conservation, Northwest A&F University, Yangling 712100, Shaanxi,China
    4.Institute of Earth Environment, Chinese Academy of Sciences, Xi'an 710075, China
  • Received:2012-04-25 Online:2013-07-01 Published:2013-04-17

PDF

1208

Cited

1

Abstract: 【Objective】 Research on the effects of soil pH on soil microbial biomass is important for providing direct insight into phosphorus transformation mechanism and suggesting biological control to enhance the P utilization rate.【Method】This experiment researched the soil microbial carbon phosphorus ratio and phosphorus availability under different soil pH conditions by testing the soil chemical and biochemical indexes in Rothamsted Research Station, United Kingdom. Two different Bray methods and Olsen method were compared to test soil microbial phosphorus in acid soil. 【Result】The results showed that soil microbial carbon phosphorus ratio could be used as a useful indicator to soil phosphorus availability. In general, soil microbial carbon phosphorus ratio had a correlation with soil pH, total carbon, inorganic phosphorus and phosphorus recovery. Soil pH affected soil chemical and soil biochemical properties. Phosphorus recovery and soil microbial carbon phosphorus ratio increased with soil pH. However, soil total carbon, total phosphorus, soil carbon phosphorus ratio and soil microbial phosphorus had a reverse trend. Soil respiration linear increased with the incubation time. 【Conclusion】Soil microbial carbon has a significant positive correlation with ATP (r=0.912, n=16). Bray-1 method is more suitable under acid soil condition for it has a small stand error, despite the result of Olsen and Bray-1 method has a good correlation. Soil pH has some effects on the transformation process from soil microbial phosphorus to inorganic phosphorus.

Key words: soil microbial biomass , carbon phosphorus ratio , long-term experiment , phosphorus availability

[1]Jenkinson D S, Oades J M. A method for measuring adenosine triphosphate in soil. Soil Biology and Biochemistry, 1979, 11:193-199.

[2]Makarov M I, Haumaier L, Zech W, Marfeninaa O E, Lysak L V. Can P-31 NMR spectroscopy be used to indicate the origins of soil organic phosphates? Soil Biology and Biochemistry, 2005, 37(1): 15-25.

[3]Amador J A, Jones R D. Response of carbon mineralization to combined changes in soil moisture and carbon-phosphorus ratio in a low phosphorus disposal. Soil Science, 1997, 162(4):275-282.

[4]Chen G C, He Z L. Determination of soil microbial biomass phosphorus in acid red soils from southern China. Biology and Fertility of Soils, 2004(39): 446-451.

[5]Sharma P, Rai S C, Sharma R, Sharmac E. Effects of land-use change on soil microbial C, N and P in a Himalayan watershed. Pedobiologia, 2004, 48(1): 83-92.

[6]McDowell R W, Mahieu N, Brookes P C, Poulton P R. Mechanisms of phosphorus solubilisation in a limed soil as a function of pH. Chemosphere, 2003, 51: 685-692.

[7]Devau N, Hinsinger P. Fertilization and pH effects on processes and mechanisms controlling dissolved inorganic phosphorus in soils. Geochimica et Cosmochimica Acta, 2011, 75: 2980-2996.

[8]Cleveland C C, Liptzin D. C: N: P stoichiometry in soil: is there a “Redfield ratio” for the microbial biomass. Biogeochemistry, 2007, 85(3): 235-252.

[9]Vance E D, Brookes P C, Jenkinson D S. An extraction method for measuring soil microbial biomass C. Soil Biology and Biochemistry, 1987, 19: 703-707.

[10]林启美, 吴玉光, 刘焕龙. 熏蒸法测定土壤微生物量碳的改进. 生态学杂志, 1999, 18(2): 63-66.

Lin Q M, Wu Y G, Liu H L. Modification of fumigation extraction method for measuring soil microbial biomass carbon. Chinese Journal of Ecology, 1999, 18(2): 63-66. (in Chinese)

[11]Brookes P C, Powlson D S, Jenkinson D S. Measurement of microbial biomass phosphorus in soil. Soil Biology and Biochemistry, 1982, 14: 319-329.

[12]吴金水, 肖和艾, 陈桂秋, 黄敏. 旱地土壤微生物磷测定方法研究. 土壤学报, 2003, 40: 70-78.

Wu J S, Xiao H A, Chen G Q, Huang M. Measurement of microbial biomass P in upland soils in china. Acta Pedologica Sinica, 2003, 40: 70-78. (in Chinese)

[13]张宝贵, 李贵桐. 土壤生物在土壤磷有效化中的作用. 土壤学报, 1998, 35(1): 104-111.

Zhao B G, Li G T. Roles of soil organisms on the enhancement of plant availability of soil phosphorus. Acta Pedologica Sinica, 1998, 35(1): 104-111. (in Chinese)

[14]来璐, 郝明德, 王永功. 黄土高原旱地长期轮作与施肥土壤微生物量磷的变化. 植物营养与肥料学报, 2004, 10(5): 546-549.

Lai L, Hao M D, Wang Y G. Changes of long-term rotation and fertilization on soil microbial phosphorus under dryland in Loess Plateau. Plant Nutrition and Fertilizing Science, 2004, 10(5): 546-549. (in Chinese)

[15]Kouno K, Wu J, Brookes P C. Turnover of biomass C and P in soil following incorporation of glucose or ryegrass. Soil Biology and Biochemistry, 2002, 34: 617-622.

[16]林启美. 土壤可溶性无机磷对微生物量磷测定的干扰. 生态学报, 2001, 21(6): 993-996.

Lin Q M. Interference of soil soluble inorganic P in measurement of soil microbial biomass P. Acta Ecologica Sinica, 2001, 21(6): 993-996. (in Chinese)

[17]Jonasson S, Michelsen A, Schmidt I K, Nielsen E V, Callaghan T V. Microbial biomass C, N and P in two artic soils and response to addition of NPK fertilizer and sugar: implications for plant uptake. Oecologia, 1996, 106: 507-515.

[18]来璐, 赵小蓉, 李贵桐, 林启美. 土壤微生物量磷及碳磷比对加入无机磷的响应. 中国农业科学, 2006, 39(10): 2036-2041.

Lai L, Zhao X R, Li G T, Lin Q M. The changes of soil     microbial biomass P and C/P with adding different quantities of inorganic P. Scientia Agricultura Sinica, 2006, 39(10): 2036-2041. (in Chinese)

[19]来璐, 赵小蓉, 李贵桐, 林启美. 低碳条件下土壤微生物量磷对加入无机磷的响应. 生态环境, 2007, 16(3): 1014-1017.

Lai L, Zhao X R, Li G T, Lin Q M. Response of soil microbial biomass P to addition of different quantities of inorganic P with low soil organic C content. Ecology and Environment, 2007, 16(3): 1014-1017. (in Chinese)

[20]Joergensen R G, Scheu S. Response of soil microbial microorganisms to the addition of carbon, nitrogen and phosphorus in a forest rendzina. Soil Biology and Biochemistry, 1999, 31: 859-866.

[21]Cleveland C C, Townsend A R. Nutrient additions to a tropical rain forest drives substantial soil carbon dioxide losses to the atmosphere. PANS, 2006, 103: 10316-10321.

[22]Parfitt R L. Phosphate adsorption on an oxisol. Soil Science Society of America Journal, 1977, 41:1064-1067.

[23]王光火, 朱祖祥, 袁可能. 红壤对磷吸附机理的初步研究. 科技通报,1989, 5(4): 31-35.

Wang G H, Zhu Z X, Yuan K N. A preliminary study on phosphorus adsorption characteristics of red soil. Bulletin of Science and Technology, 1989, 5(4): 31-35. (in Chinese)

[24]Huang P M, Berthelin J, Bollag J M, Megill W B, Page A L. Adsorption of phosphate on variable charge minerals: competitive effect of organic ligands. Environmental Impact of Soil Component Interactions, 1995, 2: 29-38.

[25]陆文龙, 曹一平, 张福锁. 根分泌的有机酸对土壤磷和微量元素的活化作用. 应用生态学报, 1999, 10(3): 379-382.

Lu W L, Cao Y P, Zhang F S. Role of root exuded organic acids in mobilization of soil phosphorus and micronutrients. Chinese Journal of Applied Ecology, 1999, 10(3): 379-382. (in Chinese)

[26]Joergensen R G, Kubler H, Meyer B, Volkmar W. Microbial biomass phosphorus in soils of beech (Fagus sylvatica L.) forests. Biology and Fertility of Soils, 1995, 19: 215-219.

[27]Lukito H P, Kouno K, Ando T. Phosphorus requirement of microbial biomass in a regosol and an andsol. Soil Biology and Biochemistry, 1998, 30: 865-872.

[28]Brookes P C, Poelson D S, Jenkinson D S. Phosphorus in the soil microbial biomass. Soil Biology and Biochemistry, 1984, 16: 169-175.

[29]Chauhan B S, Stewart J W, Paul E. Effect of labile inorganic phosphate status and organic carbon additions on the microbial uptake of phosphorus in soils. Canadian Journal of Soil Science, 1981, 61: 373-385.

[30]Ghoshal N, Singh K P. Effect of farmyard manure and inorganic fertilizer on the dynamics of soil microbial biomass in a tropical dryland agro-ecosystem. Biology and Fertility of Soils, 1995, 19: 231-238.

[31]Pereira H, Lemos P C, Reis A M, Crespo J P, Carrondo M J, Santos H. Model for carbon metabolism in biological phosphorus removal processes based on in vivo 13C-NMR labeling experiments. Water Research, 1996, 30: 2128-2138.

[32]Cleveland C C, Townsend A R, Constance B C. Soil microbial dynamics in Costa Rica: seasonal and biogeochemical constraints. Biotropical, 2004, 36(2): 184-195.

[33]Llstedt U, Singh S. Nitrogen and phosphorus limitation of microbial respiration in a tropical phosphorus-fixing acrisol (ultisol) compared with organic compost. Soil Biology and Biochemistry, 2005, 37: 1407-1410.
[1] LU Peng,LI WenHai,NIU JinCan,BATBAYAR Javkhlan,ZHANG ShuLan,YANG XueYun. Phosphorus Availability and Transformation of Inorganic Phosphorus Forms Under Different Organic Carbon Levels in a Tier Soil [J]. Scientia Agricultura Sinica, 2022, 55(1): 111-122.
[2] LI ShuaiShuai, GUO JunJie, LIU WenBo, HAN ChunLong, JIA HaiFei, LING Ning, GUO ShiWei. Influence of Typical Rotation Systems on Soil Phosphorus Availability Under Different Fertilization Strategies [J]. Scientia Agricultura Sinica, 2022, 55(1): 96-110.
[3] YanLing LIU,Yu LI,Yan ZHANG,YaRong ZHANG,XingCheng HUANG,Meng ZHANG,WenAn ZHANG,TaiMing JIANG. Characteristics of Microbial Biomass Phosphorus in Yellow Soil Under Long-Term Application of Phosphorus and Organic Fertilizer [J]. Scientia Agricultura Sinica, 2021, 54(6): 1188-1198.
[4] ShiChao WANG,ZhiHao YAN,JinYu WANG,ShengChang HUAI,HongLiang WU,TingTing XING,HongLing YE,ChangAi LU. Nitrogen Fertilizer and Its Combination with Straw Affect Soil Labile Carbon and Nitrogen Fractions in Paddy Fields [J]. Scientia Agricultura Sinica, 2020, 53(4): 782-794.
[5] LI RuoNan,WANG ZhengPei,BATBAYAR Javkhlan,ZHANG DongJie,ZHANG ShuLan,YANG XueYun. Relationship Between Soil Available Phosphorus and Inorganic Phosphorus Forms Under Equivalent Organic Matter Condition in a Tier Soil [J]. Scientia Agricultura Sinica, 2019, 52(21): 3852-3865.
[6] SHI Ke, DONG ShiGang, SHEN FengMin, LONG Qian, JIANG GuiYing, LIU Fang, LIU ShiLiang. Effects of Wheat Seeding Rate with Nitrogen Fertilizer Application Reduction on Soil Microbial Biomass Carbon, Nitrogen and Enzyme Activities in Fluvo-Aquic Soil in Huang-Huai Plain [J]. Scientia Agricultura Sinica, 2019, 52(15): 2646-2663.
[7] REN FengLing, ZHANG XuBo, SUN Nan, XU MingGang, LIU KaiLou. A Meta-Analysis of Manure Application Impact on Soil Microbial Biomass Across China’s Croplands [J]. Scientia Agricultura Sinica, 2018, 51(1): 119-128.
[8] WANG ChuanJie, XIAO Jing, CAI AnDong, ZHANG WenJu, XU MingGang. Capacity and Characteristics of Soil Microbial Biomass Under Various Climate and Fertilization Conditions Across China Croplands [J]. Scientia Agricultura Sinica, 2017, 50(6): 1067-1075.
[9] YANG Xin-yi, LIU Xiao-hu, HAN Xiao-ri, DUAN Peng-peng, ZHU Yu-cui, QI Wen . Responses of Soil Microbial Biomass and Soluble Organic Matter to Different Application Rates of N：A Comparison Between Liaochun 10 and Liaochun 18 [J]. Scientia Agricultura Sinica, 2016, 49(7): 1315-1324.
[10] SHANG Jie, GENG Zeng-chao, WANG Yue-ling, CHEN Xin-xiang, ZHAO Jun. Effect of Biochar Amendment on Soil Microbial Biomass Carbon and Nitrogen and Enzyme Activity in Tier Soils [J]. Scientia Agricultura Sinica, 2016, 49(6): 1142-1151.
[11] YANG Lin-sheng, ZHANG Yu-ting, HUANG Xing-cheng, ZHANG Yue-qiang, ZHAO Ya-nan, SHI Xiao-jun. Effects of Long-Term Application of Chloride Containing Fertilizers on the Biological Fertility of Purple Soil Under a Rice-Wheat Rotation System [J]. Scientia Agricultura Sinica, 2016, 49(4): 686-694.
[12] YANG Xin-yi, LIU Xiao-hu, HAN Xiao-ri. Effect of Nitrogen Application Rates in Different Fertility Soils on Soil N Transformations and N Use Efficiency Under Different Fertilization Managements [J]. Scientia Agricultura Sinica, 2016, 49(13): 2561-2571.
[13] LIANG Bin, ZHAO Wei, YANG Xue-Yun, ZHOU Jian-Bin. Nitrogen Retention and Supply After Addition of N Fertilizer and Its Combination with Straw in the Soils with Different Fertilities [J]. Scientia Agricultura Sinica, 2012, 45(9): 1750-1757.
[14] LIANG Bin, ZHAO Wei, YANG Xue-Yun, ZHOU Jian-Bin. Effects of Long-Term Different Fertilization Managements on Changes of N in Soil and Its Uptake by Wheat on Dryland [J]. Scientia Agricultura Sinica, 2012, 45(5): 885-892.
[15] LU Mei-Rong , LI Zhong-Pei, LIU Ming, JIANG Chun-Yu, CHE Yu-Ping. Soil Microbial Biomass N Turnover After Long-Term Fertilization in Paddy Field [J]. Scientia Agricultura Sinica, 2012, 45(2): 275-282.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd