Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (11): 2370-2376.doi: 10.3864/j.issn.0578-1752.2013.11.022

• VETERINARY SCIENCE • Previous Articles     Next Articles

Inhibitory Activity of Chinese Herbal Aqueous Extracts to Tetracycline-Resistant E. coli of Swine

 HUANG  Ming-Qian, KONG  Xiang-Feng, XIAO  Li-Chun, GUO  Xiao-Quan   

  1. 1.College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045
    2.Institute of Subtropical Agriculture, Chinese Academy of Sciences/Hunan Engineering and Research Center of Animal and Poultry Science, Changsha 410125
  • Received:2012-12-11 Online:2013-06-01 Published:2013-03-21

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Abstract: 【Objective】 This study was conducted to determine the inhibitory activity of Chinese herbal aqueous extracts to tetracycline-resistant E. coli of swine in order to provide a basis for their application in the swine production. 【Method】 Paper disk method and PCR method were used to evaluate tetracycline sensitivity to 10 isolates of E. coli of swine and their tetracycline-resistance genes, respectively, as well as the agar diffusion and double dilution to test the inhibitory activity of Chinese herbal aqueous extracts and their mixtures with tetracycline to 10 isolates of resistant E. coli of swine. 【Result】 All of the 10 tested isolates were resistant to tetracycline, 10 of which carried the tet A, two of which carried tet B, and 9 of which carried tet C. The extracts of Fructus crataegi, Fructus forsythiae, Flos lonicerae and Fructus schisandrae had stronger antibacterial activity, while their mixtures with tetracycline presented the decreased inhibition zone diameter and increased minimal inhibitory concentration. 【Conclusion】 The tetracycline resistance of the tested strains was related to the existances of resistance genes, including tetA, tetB and tetC. The extracts of 4 Chinese herbs tested in this study had stronger inhibitory activity against the isolates of E. coli, and could not be used with the tetracycline togather.

Key words: E. coli , Chinese herbal aqueous extract , tetracycline , resistance

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