Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (7): 1516-1522.doi: 10.3864/j.issn.0578-1752.2013.07.023

• RESEARCH NOTES • Previous Articles    

The Extraction and Isolation Method of Nitrosyl Hemochromogen

 SUN  Wei-Qing, ZHOU  Guang-Hong, XU  Xing-Lian   

  1. 1.College of Life Science, Yangtze University, Jingzhou 434025, Hubei
    2. Key Laboratory of Meat Processing and Quality Control, Ministry of Education, Nanjing Agricultural University, Nanjing 210095
  • Received:2012-10-31 Online:2013-04-01 Published:2013-01-30

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Abstract: 【Objective】The purpose of the experiment is to obtain high purity nitrosyl hemochromogen (NH). 【Method】Seven kinds of different polarity organic solvents were used to extract and isolate the NH. The UV and EPR spectral characteristics of extracted solution and the hue of meat residue after being extracted NH were analyzed. 【Result】 NH is insoluble in petroleum ether completely. Acetone can dissolve NH efficiently and the extracted solution is relatively stable. Extraction rate of ethyl acetate is not high, but which can keep the pink of NH. The EPR spectrum of NH extracted by acetone was showed the signal information of free radical not of the NH because of the presence of the lipid. For these results, a three-step gradational extraction and isolation procedure with petroleum ether/acetone/ethyl acetate step by step was established based on the characteristics of UV and EPR spectroscopy of NH solution. The diced sample was extracted with 3 times the petroleum ether ( boiling range is 30-60℃) in the dark, anaerobic conditions for 36 h, discard the petroleum ether, and then add 1.5 times the acetone extraction for 10 min, filter to obtain bright red solution. The acetone was removed and the NH was isolated with ethyl acetate again.【Conclusion】It indicated that the three-step gradational extraction procedure with petroleum ether/acetone/ethyl acetate step by step is an effective method for isolation of NH.

Key words: nitrosyl hemochromogen , extraction , isolation , UV spectrum , EPR spectrum

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