Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (5): 1025-1035.doi: 10.3864/j.issn.0578-1752.2013.05.018

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Effect of Corn Straw Diet on Diversity of the Bacterial Community in the Rumen of Dairy Cows Analyzed by DGGE

 JIN  Di, WANG  Jia-Qi, BU  Deng-Pan, ZHAO  Sheng-Guo, HU  Xiao-Li, ZHAO  Jing-Wen, LU  Yu-Fei, WANG  Dan-Dan, SUN  Peng   

  1. Institute of Animal Science, Chinese Academy of Agricultural Sciences/State Key Laboratory of Animal Nutrition, Beijing 100193
  • Received:2012-09-28 Online:2013-03-01 Published:2012-12-20

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Abstract: 【Objective】The purpose of this study is to reveal the unique rumen bacterial community under the diet of the corn straw compared to the diet of alfalfa fed to dairy cows. 【Method】Twelve healthy Chinese Holstein dairy cows with permanent rumen cannula and similar live weight were assigned to two treatments, corn straw diet (CS) and mixed forage (alfalfa, Chinese wildrye hay and silage maize) diet (MF). The solid and liquid fractions of rumen digesta were collected for the successive three days on the 13th week of the experiment. Total DNA was extracted and analyzed by denaturing gradient gel electrophoresis (DGGE) with subsequent cluster and sequence analysis. 【Result】 DGGE profiles showed the quantity and optimal density of the DGGE bands in CS group were different from MF group. Some bands from CS group were less compared to MF group. Shannon-Weiner index showed no difference (P＞0.05) between the two groups for liquid-associated bacteria, but it was significantly lower (P＜0.01) in CS group than that in MF group for solid-associated bacteria. Analysis from sequences showed that the diversity of Prevotella sp., Acetivibrio ethanolgignens and Clostridium sp. decreased in CS group. The other uncultured bacteria belonging to the phylum Bacteroidetes, Firmicutes, Tenericutes and Proteobacteria had some changes between the two groups.【Conclusion】 There are unique bacterial community and low diversity in the rumen of dairy cows fed with diet of corn straw.

Key words: corn straw , type of forage , rumen , bacteria community , DGGE

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