Scientia Agricultura Sinica ›› 2018, Vol. 51 ›› Issue (23): 4548-4555.doi: 10.3864/j.issn.0578-1752.2018.23.013

• ANIMAL SCIENCE·VETERINARY SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

The Effects of Artemisia annua Extracts on the Rumen Fermentation in Dairy Cows

WANG LiFang1(), 2,AO ChangJin2   

  1. 1Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031
    2Inner Mongolia Agricultural University, Hohhot 010018
  • Received:2018-02-12 Accepted:2018-06-12 Online:2018-12-01 Published:2018-12-12

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Abstract:

【Objective】The effects of daisy plant (Artemisia annua) extracts (AAE) on the fermentation indexes such as acetic acid, propionic acid and butyric acid in the rumen in dairy cows were studied in this paper, and furthermore the effects on B. fibrisolvens and C. proteoclasticum for regulating CLA synthesis in the rumen were researched. The purpose of this study was to explore the effects of AAE on rumen fermentation in dairy cows, and to investigate the part mechanism of effecting CLA synthesis from the rumen.【Method】Fifteen dairy cows (600±29) kg, parity 2-3, in lactating (158±3) d and milk yield (22.8±1.8)kg·d -1 were used in completely random design in 40 d experimental periods. The experimental dairy cows were integrated management, free ingestion, free drinking water, feeding 2 times per day, milking 2 times (4:30 a.m., 16:30 p.m.). The experiments groups comprised the control and two treatments (treatment one and treatment two, the doses of AAE for supplementation was 96 g·d -1 and 160 g·d -1 for every cow, respectively). The experimental period comprised a 9-d adaptation and a 31-d the formal period. 【Result】 The results showed as follows: ruminal pH values tended to increase in two treatments for AAE supplementation compared with the control, which increased by 2.63% and 8.61%, respectively (P>0.05). The concentrations for acetic acid, propionic acid and butyric acid decreased significantly in two treatments except for isovaleric acid and valeric acid in the low dose of AAE compared with the control (P<0.05). AAE supplementation increased the relative percentage of B. fibrisolvens in rumen mixture and rumen liquid (P<0.05), and decreased the relative percentage of B. fibrisolvens in rumen solid (P>0.05). AAE supplementation decreased the relative percentage of C. proteoclasticum in rumen liquid and rumen solid (P<0.05), and increased the relative percentage of C. proteoclasticum in rumen mixture (P>0.05). 【Conclusion】 It was concluded that AAE supplementation had adverse effect on rumen fermentation characteristics, and had different effects on B. fibrisolvens and C. proteoclasticum on different contents of the rumen, but the general trend was increasing the relative percentage of B. fibrisolvens, and decreased the relative percentage of C. proteoclasticum, which ws beneficial to CLA synthesis.

Key words: Artemisia annua extracts, dairy cows, rumen, B. fibrisolvens, C. proteoclasticum

Table 1

Composition and nutrient levels of the basal diet (DM basis)"

饲粮组成（DM） Diet composition 含量 Content (%) 营养水平 Nutrient levels
原料Ingredients 干物质DM(kg/（d·头）) 13.5
精饲料 Concentrated feed 53.2 产奶净能NEL(MJ·kg-1) 6.4
苜蓿 Alfalfa 10.9 粗蛋白 CP（%） 14.2
羊草 Leymus chinensis 3.6 粗脂肪 EE（%） 2.94
青贮 Silage 25.1 中性洗涤纤维NDF（%） 42.5
谷草 Millet straw 7.2 钙 Ca（%） 0.83
磷 P（%） 0.65

Table 2

Primer sequence of biohydrogenation associated bacteria used for real-time PCR"

细菌 Target bacterium 序列(5’ to 3’) Primer sequence 扩增片段Product size（bp） 退火温度Annealing temperature (℃)
瘤胃总菌 Total bacteria F:CGGCAACGAGCGCAACCC
R:CCATTGTAGCACGTGTGTAGCC		 147 55
溶纤维丁酸弧菌 B.fibrisolvens F:GCCTCAGCGTCAGTAATCG
R:GGAGCGTAGGCGGTTTTAC		 187 55
蛋白溶解梭菌 C.proteoclasticum F:TCCGGTGGTATGAGATGGGC
R:GTCGCTGCATCAGAGTTTCCT		 185 55

Table 3

PCR system"

试剂 Reagents 体积 Volume (μL)
SRBR Premix Ex TaqTMⅡ（2×） 12.5
正向引物Forward 1
反向引物Reverse 1
DNA 5
ddH2O to 25

Table 4

The effects of artemisia annua extracts on ruminal fluid pH value and VFA in vivo (mmol·L-1)"

项目
Items		 对照组
Control group		 试验1组
Experimental group 1		 试验2组
Experimental group 2		 SEM P 值
P value
pH 6.85 7.03 7.44 0.24 0.116
乙酸 Acetate acid 42.41a 27.03b 18.13c 1.75 <0.0001
丙酸 Propionic acid 12.42a 8.93b 6.73b 1.02 0.004
丁酸 Butyrate 7.00a 5.75b 4.00c 0.24 <0.0001
异戊酸 Isovaleric acid 1.67a 1.28ab 0.80c 0.21 0.0165
戊酸 Valerate 0.80a 0.76a 0.40b 0.05 0.0003

Table 5

The effects of Artemisia annua extracts on ruminal B.fibrisolvens in vivo"

对照组
Control group		 试验1组
Experimental group 1		 试验2组
Experimental group 2		 P 值
P value
瘤胃液相 Rumen liquid 0.27b 0.37a 0.38a 0.0325
瘤胃固相 Rumen solid 0.16 0.14 0.13 0.8451
瘤胃液全混合物 Rumen mixture 0.18b 0.33a 0.28a 0.0127

Table 6

The effects of Artemisia annua extracts on ruminal C.proteoclasticum in vivo"

对照组
Control group		 试验1组
Experimental group 1		 试验2组
Experimental group 2		 P 值
P value
瘤胃液相 Rumen liquid 1.79 1.70 1.77 0.9661
瘤胃固相 Rumen solid 2.38 1.84 2.28 0.4201
瘤胃液全混合物 Rumen mixture 1.56b 2.41a 1.82ab 0.0601
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